EA200901123A1 - Флуоресцентные индоцианиновые красители и их производные для анализа биологических макромолекул - Google Patents

Флуоресцентные индоцианиновые красители и их производные для анализа биологических макромолекул

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Publication number
EA200901123A1
EA200901123A1 EA200901123A EA200901123A EA200901123A1 EA 200901123 A1 EA200901123 A1 EA 200901123A1 EA 200901123 A EA200901123 A EA 200901123A EA 200901123 A EA200901123 A EA 200901123A EA 200901123 A1 EA200901123 A1 EA 200901123A1
Authority
EA
Eurasian Patent Office
Prior art keywords
dyes
derivatives
analysis
relative
fluorescence
Prior art date
Application number
EA200901123A
Other languages
English (en)
Inventor
Александр Васильевич Чудинов
Виктория Евгеньевна Кузнецова
Александр Сергеевич ЗАСЕДАТЕЛЕВ
Виктор Евгеньевич БАРСКИЙ
Original Assignee
Учреждение Российской Академии Наук Институт Молекулярной Биологии Им. В.А. Энгельгардта Ран (Имб Ран)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Учреждение Российской Академии Наук Институт Молекулярной Биологии Им. В.А. Энгельгардта Ран (Имб Ран) filed Critical Учреждение Российской Академии Наук Институт Молекулярной Биологии Им. В.А. Энгельгардта Ран (Имб Ран)
Publication of EA200901123A1 publication Critical patent/EA200901123A1/ru

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
    • C07D209/18—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
    • C07D209/18—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C07D209/24—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals with an alkyl or cycloalkyl radical attached to the ring nitrogen atom
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
    • C09B23/083—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines five >CH- groups
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Indole Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Синтезировано два ряда индолениновых пентаметиновых красителей (37 соединений), содержащих реактивную группу в третьем и пятом положениях индоленинового цикла соответственно. Разработан способ получения, выделения и очистки красителей и их предшественников. Для заявляемых красителей определены максимумы поглощения и флуоресценции, коэффициенты молекулярной экстинкции, квантовые выходы флуоресценции, относительная эффективность флуоресценции при возбуждении на длине волны 635 нм (регистрация 670 нм) и 655 нм (регистрация 690 нм), относительная светостойкость, сравнительная чувствительность обнаружения маркированных олигонуклеотидов на биочипах. Показано применение заявляемых красителей для использования в качестве флуоресцентных меток для олигонуклеотидных и белковых микрочипов.
EA200901123A 2007-04-11 2007-04-11 Флуоресцентные индоцианиновые красители и их производные для анализа биологических макромолекул EA200901123A1 (ru)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/RU2007/000172 WO2008127139A1 (fr) 2007-04-11 2007-04-11 Colorants d'indocyanine fluorescents et leurs dérivés destinés à l'analyse de macromolécules biologiques

Publications (1)

Publication Number Publication Date
EA200901123A1 true EA200901123A1 (ru) 2010-02-26

Family

ID=39864142

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200901123A EA200901123A1 (ru) 2007-04-11 2007-04-11 Флуоресцентные индоцианиновые красители и их производные для анализа биологических макромолекул

Country Status (3)

Country Link
EP (1) EP2157088A4 (ru)
EA (1) EA200901123A1 (ru)
WO (1) WO2008127139A1 (ru)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2815018C2 (ru) * 2018-12-20 2024-03-11 Такеда Фармасьютикал Компани Лимитед Производные илида сульфоксония в качестве зондов для цистеиновой протеазы

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2204194B1 (en) * 2008-12-25 2017-08-02 Canon Kabushiki Kaisha Probe for a hair cell, and labelling method for a hair cell using the probe for a hair cell
GB201010878D0 (en) * 2010-06-29 2010-08-11 Ge Healthcare As Dye compositiion and dye syntheses
CN103052690A (zh) * 2010-06-29 2013-04-17 通用电气医疗集团股份有限公司 染料组合物和染料合成
KR101309789B1 (ko) * 2011-01-26 2013-09-23 (주)프로테옴텍 티로신 산화물이 결합된 바이오 물질을 포함하는 단백질 검출용 조성물
RU2667070C1 (ru) * 2017-09-22 2018-09-14 Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран) Дезоксиуридинтрифосфаты, связанные с цианиновыми красителями сульфамидоалкильными линкерами, для использования в ПЦР

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2649692B2 (ja) * 1988-05-18 1997-09-03 コニカ株式会社 ハロゲン化銀写真感光材料
DE19921234B4 (de) * 1999-05-07 2005-07-28 Few Chemicals Gmbh Cyanin-Farbstoffe
WO2002026891A1 (en) 2000-09-29 2002-04-04 Molecular Probes, Inc. Modified carbocyanine dyes and their conjugates
EP1211294B1 (en) 2000-11-28 2008-08-13 Visen Medical, Inc. Improved process and method for the preparation of asymmetric monofunctionalised indocyanine labelling reagents and obtained compounds
DE10108808A1 (de) * 2001-02-16 2002-09-05 Joerg Enderlein Fluoreszierende Mikroteilchen
CN100473653C (zh) * 2005-06-06 2009-04-01 大连理工大学 生物分析用近红外中位氮、硫七甲川菁类荧光染料

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2815018C2 (ru) * 2018-12-20 2024-03-11 Такеда Фармасьютикал Компани Лимитед Производные илида сульфоксония в качестве зондов для цистеиновой протеазы

Also Published As

Publication number Publication date
EP2157088A4 (en) 2010-04-14
EP2157088A1 (en) 2010-02-24
WO2008127139A1 (fr) 2008-10-23

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