EA200600102A1 - Очистка вариантов her-2 - Google Patents

Очистка вариантов her-2

Info

Publication number
EA200600102A1
EA200600102A1 EA200600102A EA200600102A EA200600102A1 EA 200600102 A1 EA200600102 A1 EA 200600102A1 EA 200600102 A EA200600102 A EA 200600102A EA 200600102 A EA200600102 A EA 200600102A EA 200600102 A1 EA200600102 A1 EA 200600102A1
Authority
EA
Eurasian Patent Office
Prior art keywords
protein
provides
variant
chromatography
cleaning options
Prior art date
Application number
EA200600102A
Other languages
English (en)
Other versions
EA008827B1 (ru
Inventor
Марие Эсклинг
Клаус Грегориус Нильсен
Original Assignee
Фармекса А/С
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Фармекса А/С filed Critical Фармекса А/С
Priority claimed from PCT/DK2004/000451 external-priority patent/WO2004113377A1/en
Publication of EA200600102A1 publication Critical patent/EA200600102A1/ru
Publication of EA008827B1 publication Critical patent/EA008827B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Настоящее изобретение предоставляет новый способ очистки белка семейства EGFR, полученного из культур клеток насекомых. Процесс содержит последовательные этапы а) диафильтрации и замены культуральной среды буфером; b) аффинной хроматографии с иммобилизованным металлом (IMAC); с) эксклюзионной хроматографии (SEC), е) анионообменной хроматографии (AIE). Способ также предоставляет иммуногенный вариант белка HER-2, для которого главным образом адаптирован процесс очистки, а также средства для получения указанного варианта.
EA200600102A 2003-06-25 2004-06-24 Очистка вариантов her-2 EA008827B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200300954 2003-06-25
PCT/DK2004/000451 WO2004113377A1 (en) 2003-06-25 2004-06-24 Purification of her-2 variants

Publications (2)

Publication Number Publication Date
EA200600102A1 true EA200600102A1 (ru) 2006-06-30
EA008827B1 EA008827B1 (ru) 2007-08-31

Family

ID=37142866

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200600102A EA008827B1 (ru) 2003-06-25 2004-06-24 Очистка вариантов her-2

Country Status (6)

Country Link
US (2) US7763452B2 (ru)
KR (1) KR20060033870A (ru)
CN (1) CN101094864A (ru)
EA (1) EA008827B1 (ru)
IL (1) IL171903A (ru)
ZA (1) ZA200509951B (ru)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA008827B1 (ru) * 2003-06-25 2007-08-31 Фармекса А/С Очистка вариантов her-2
EP2596801B1 (en) * 2006-10-06 2018-05-02 Bavarian Nordic A/S Recombinant modified vaccinia ankara virus encoding a her-2 antigen for use in treating cancer
WO2009149094A2 (en) * 2008-06-02 2009-12-10 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Cytotoxic t cell defined egfr peptide and an optimized derivative peptide
CN102234332B (zh) * 2010-04-26 2014-12-17 浙江海正药业股份有限公司 一种重组人血白蛋白及其融合蛋白的分离纯化工艺
CA2798837A1 (en) * 2010-06-07 2011-12-15 Pfizer Inc. Her-2 peptides and vaccines
WO2012051147A1 (en) * 2010-10-11 2012-04-19 Abbott Laboratories Processes for purification of proteins
JP6824555B2 (ja) * 2015-03-31 2021-02-03 サイティバ・スウェーデン・アクチボラグ 液体クロマトグラフィーシステム、装置、および方法
CN109734799A (zh) * 2018-07-23 2019-05-10 深圳市伯劳特生物制品有限公司 一种表达和纯化Her2全长蛋白的方法以及Her2全长蛋白的应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK96493D0 (da) 1993-08-26 1993-08-26 Mouritsen Og Elsner Aps Fremgangsmaade til at inducere antistofresponser mod selvproteiner og autovaccine fremstillet ved fremgangsmaaden
US6080409A (en) 1995-12-28 2000-06-27 Dendreon Corporation Immunostimulatory method
EA003634B1 (ru) * 1998-10-05 2003-08-28 Фармэкса А/С Новые способы терапевтической вакцинации
WO2000044899A1 (en) 1999-01-29 2000-08-03 Corixa Corporation Her-2/neu fusion proteins
US20030228606A1 (en) * 2002-04-11 2003-12-11 Amgen Inc., A Corporation Of The State Of Delaware Her-2 receptor tyrosine kinase molecules and uses thereof
EA008827B1 (ru) 2003-06-25 2007-08-31 Фармекса А/С Очистка вариантов her-2
MXPA05013389A (es) 2003-06-25 2006-03-17 Pharmexa As Purificacion de variantes her-2.

Also Published As

Publication number Publication date
IL171903A0 (en) 2006-04-10
US20100285071A1 (en) 2010-11-11
US20060240511A1 (en) 2006-10-26
CN101094864A (zh) 2007-12-26
US8022039B2 (en) 2011-09-20
KR20060033870A (ko) 2006-04-20
US7763452B2 (en) 2010-07-27
EA008827B1 (ru) 2007-08-31
ZA200509951B (en) 2006-11-29
IL171903A (en) 2013-12-31

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ KZ KG MD TJ TM

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): BY RU