EA200500395A1 - Способы и композиции обнаружения и анализа полинуклеотидов с помощью светособирающих полихромофоров - Google Patents

Способы и композиции обнаружения и анализа полинуклеотидов с помощью светособирающих полихромофоров

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Publication number
EA200500395A1
EA200500395A1 EA200500395A EA200500395A EA200500395A1 EA 200500395 A1 EA200500395 A1 EA 200500395A1 EA 200500395 A EA200500395 A EA 200500395A EA 200500395 A EA200500395 A EA 200500395A EA 200500395 A1 EA200500395 A1 EA 200500395A1
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methods
compositions
light
target polynucleotide
polychromophors
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EA200500395A
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English (en)
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EA007653B1 (ru
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Гильермо С. Базан
Брент С. Гейлорд
Шу Ванг
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Дзе Риджентс Оф Дзе Юниверсити Оф Калифорния
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
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  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)

Abstract

Изобретение включает способы, композиции и промышленные изделия для анализа образца на целевой полинуклеотид. Образец, предположительно содержащий целевой полинуклеотид, контактирует с поликатионным полихромофором и сенсорным полинуклеотидом, комплементарньм целевому полинуклеотиду. Сенсорный полинуклеотид содержит сигнальный хромофор, поглощающий энергию возбуждённого полихромофора и повышающий эмиссию (излучение) света в присутствии целевого полинуклеотида. Способы могут применяться в сложной форме. Также включаются наборы, содержащие реагенты для осуществления таких способов.Отчет о международном поиске был опубликован 2005.05.12.
EA200500395A 2002-08-26 2003-08-26 Способы и композиции обнаружения и анализа полинуклеотидов с помощью светособирающих полихромофоров EA007653B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US40626602P 2002-08-26 2002-08-26
PCT/US2003/026989 WO2004092324A2 (en) 2002-08-26 2003-08-26 Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
US10/648,945 US7270956B2 (en) 2002-08-26 2003-08-26 Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores

Publications (2)

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EA200500395A1 true EA200500395A1 (ru) 2005-10-27
EA007653B1 EA007653B1 (ru) 2006-12-29

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EA200500395A EA007653B1 (ru) 2002-08-26 2003-08-26 Способы и композиции обнаружения и анализа полинуклеотидов с помощью светособирающих полихромофоров

Country Status (9)

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US (9) US7270956B2 (ru)
EP (1) EP2316971A1 (ru)
JP (2) JP4504821B2 (ru)
KR (1) KR20050055717A (ru)
CN (2) CN102021243A (ru)
AU (1) AU2003304043A1 (ru)
CA (1) CA2497152C (ru)
EA (1) EA007653B1 (ru)
WO (1) WO2004092324A2 (ru)

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US8617814B2 (en) 2013-12-31
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CN1694967B (zh) 2010-12-15
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CA2497152A1 (en) 2004-10-28
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US8227187B2 (en) 2012-07-24
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US20080064042A1 (en) 2008-03-13
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US9085799B2 (en) 2015-07-21

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