EA200300426A1 - Способы получения парагидроксибензоата в бактериях - Google Patents

Способы получения парагидроксибензоата в бактериях

Info

Publication number
EA200300426A1
EA200300426A1 EA200300426A EA200300426A EA200300426A1 EA 200300426 A1 EA200300426 A1 EA 200300426A1 EA 200300426 A EA200300426 A EA 200300426A EA 200300426 A EA200300426 A EA 200300426A EA 200300426 A1 EA200300426 A1 EA 200300426A1
Authority
EA
Eurasian Patent Office
Prior art keywords
phba
obtaining
important
tolerant
strains
Prior art date
Application number
EA200300426A
Other languages
English (en)
Inventor
Хуан Л. Рамос
Ари Бен-Бассат
Патрисия Годой
М. Изабель Рамос-Гонсалес
Эстрелла Дюк
Original Assignee
Норт Каролина Стейт Юниверсити
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Норт Каролина Стейт Юниверсити filed Critical Норт Каролина Стейт Юниверсити
Publication of EA200300426A1 publication Critical patent/EA200300426A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Данное изобретение относится к выделению нового оперона tonB Pseudomonas putida. Указанные гены применимы для того, чтобы сделать клетки более чувствительными к антибиотикам, толуолу, pHBA, ароматическим соединениям, парабенам и ароматическим аминокислотам после инактивации с помощью специфичных мутантных аллелей или более толерантными к указанным соединениям после сверхэкспрессии с помощью соответствующего экспрессирующего вектора. Полученные данные имеют важное значение в области медицины и биотехнологии и биокатализа. Кроме того, представлен способ скрининга для идентификации генов толерантности к pHBA и идентифицированы особенно толерантные к pHBA штаммы. Указанные штаммы имеют важное значение для получения pHBA.Отчет о международном поиске был опубликован 2003.04.10.
EA200300426A 2000-09-30 2001-09-28 Способы получения парагидроксибензоата в бактериях EA200300426A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23687900P 2000-09-30 2000-09-30
PCT/US2001/031180 WO2002029034A2 (en) 2000-09-30 2001-09-28 Exbb-exbd-tonb gene cluster of pseudomonas putida dot-t1e conferring tolerance to aromatic compounds and resistance to antibiotics

Publications (1)

Publication Number Publication Date
EA200300426A1 true EA200300426A1 (ru) 2004-04-29

Family

ID=22891371

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200300426A EA200300426A1 (ru) 2000-09-30 2001-09-28 Способы получения парагидроксибензоата в бактериях

Country Status (6)

Country Link
US (1) US7348421B1 (ru)
EP (1) EP1320594A2 (ru)
AP (1) AP2003002768A0 (ru)
AU (1) AU2002213028A1 (ru)
EA (1) EA200300426A1 (ru)
WO (1) WO2002029034A2 (ru)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1589112A1 (en) * 2004-04-21 2005-10-26 Nederlandse Organisatie voor toegepast-natuurwetenschappelijk onderzoek TNO Microbial production of aromatic acids
BRPI0609093B1 (pt) 2005-05-11 2018-12-18 Chr Hansen As processo para preparar uma cepa de bifidobacterium sp. que contém um gene tetw mutante em seu cromossomo, cepa de bifidobacterium e uso da mesma
WO2007086618A1 (en) 2006-01-30 2007-08-02 Ajinomoto Co., Inc. L-amino acid producing bacterium and method of producing l-amino acid
KR20140022694A (ko) 2012-08-14 2014-02-25 삼성전자주식회사 생물학적 방법으로 파라-히드록시벤조산을 제조하는 방법
CN107118973B (zh) * 2017-01-20 2019-10-22 厦门欧米克生物科技有限公司 一种利用微生物生产对羟基苯甲醛的方法
WO2020257524A1 (en) * 2019-06-21 2020-12-24 Bio Capital Holdings, LLC Uv-resistant biological devices and extracts and methods for producing and using the same

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4657863A (en) 1982-07-02 1987-04-14 Celanese Corporation Stabilization of a mutant microorganism population
US4968612A (en) 1984-07-27 1990-11-06 Celgene Corporation Continuous fermentation process for aromatic hydrocarbon bioconversion
US4810648A (en) 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
JPH05328981A (ja) 1992-06-04 1993-12-14 Kawasaki Steel Corp パラヒドロキシ安息香酸の製造方法
JPH05336979A (ja) 1992-06-05 1993-12-21 Kawasaki Steel Corp パラヒドロキシ安息香酸の製造方法
JPH05336980A (ja) 1992-06-05 1993-12-21 Kawasaki Steel Corp パラヒドロキシ安息香酸の製造方法
FR2694571B1 (fr) 1992-08-10 1994-11-04 Rhone Poulenc Chimie Polypeptides possédant une activité nitrilase, séquence d'ADN codant pour lesdits polypeptides, cassettes d'expression et micro-organismes hôtes permettant leur obtention.
US5629190A (en) 1992-08-10 1997-05-13 Rhone-Poulenc Chimie Polypeptides possessing a nitrilase activity and method of converting nitriles to carboxylates by means of said polypeptides
JP3154633B2 (ja) 1994-12-28 2001-04-09 三菱レイヨン株式会社 ニトリラーゼ遺伝子発現のための調節因子およびその遺伝子
US5858736A (en) 1996-05-17 1999-01-12 E. I. Du Pont De Nemours And Company Preparation of lactams from aliphatic α,ω-dinitriles
US6830899B1 (en) 1997-06-13 2004-12-14 E. I. Du Pont De Nemours And Company Method for the production of para-hydroxybenzoate in Pseudomonas mendocina
US6410265B1 (en) * 1997-10-31 2002-06-25 E. I. Du Pont De Nemours And Company Gene encoding a putive efflux protein for solvents/antibiotics in Pseudomonas mendocina
US6030819A (en) 1998-09-28 2000-02-29 General Electric Company Genetically engineered microorganisms and method for producing 4-hydroxybenzoic acid

Also Published As

Publication number Publication date
AP2003002768A0 (en) 2003-03-31
WO2002029034A3 (en) 2003-04-10
US7348421B1 (en) 2008-03-25
WO2002029034A2 (en) 2002-04-11
EP1320594A2 (en) 2003-06-25
AU2002213028A1 (en) 2002-04-15

Similar Documents

Publication Publication Date Title
Selmer et al. p‐Hydroxyphenylacetate decarboxylase from Clostridium difficile: a novel glycyl radical enzyme catalysing the formation of p‐cresol
Boncompagni et al. Occurrence of choline and glycine betaine uptake and metabolism in the family Rhizobiaceae and their roles in osmoprotection
Walker et al. The many roles of glutamate in metabolism
De Vos et al. Comamonas Davis and Park 1962 gen. nov., nom. rev. emend., and Comamonas terrigena Hugh 1962 sp. nov., nom. rev.
Velayudhan et al. L-serine catabolism via an oxygen-labile L-serine dehydratase is essential for colonization of the avian gut by Campylobacter jejuni
Leuthner et al. A two-component system involved in regulation of anaerobic toluene metabolism in Thauera aromatica
Yirui et al. Isolation of marine benzo [a] pyrene-degrading Ochrobactrum sp. BAP5 and proteins characterization
BR9803346B1 (pt) Cepa de microorganismos transformados, bem como processo para a preparação fermentativa de l-cisteína, l-cistina, n-acetil-serina ou derivados de tiazolidina
Heim et al. Proteome reference map of Pseudomonas putida strain KT2440 for genome expression profiling: distinct responses of KT2440 and Pseudomonas aeruginosa strain PAO1 to iron deprivation and a new form of superoxide dismutase
DE60330841D1 (de) Überexpression von extremozymgenen in pseudomonas und nahe verwandte bakterien
Valle et al. The amino acid valine is secreted in continuous-flow bacterial biofilms
Nakahigashi et al. Isolation and characterization of a light-sensitive mutant of Escherichia coli K-12 with a mutation in a gene that is required for the biosynthesis of ubiquinone
Wang et al. Roseovarius pacificus sp. nov., isolated from deep-sea sediment
Mouslim et al. Conflicting needs for a Salmonella hypervirulence gene in host and non‐host environments
Vaz-Moreira et al. Bacillus purgationiresistans sp. nov., isolated from a drinking-water treatment plant
EA200300426A1 (ru) Способы получения парагидроксибензоата в бактериях
Zhang et al. Sphingobium jiangsuense sp. nov., a 3-phenoxybenzoic acid-degrading bacterium isolated from a wastewater treatment system
Fink et al. Chromosomal expression of the Haemophilus influenzae Hap autotransporter allows fine-tuned regulation of adhesive potential via inhibition of intermolecular autoproteolysis
Hornstein et al. Oxacillin-hydrolyzing β-lactamase involved in resistance to imipenem in Acinetobacter baumannii
CN105829522B (zh) 微生物
Konan et al. Role of ribosome release in regulation of tna operon expression in Escherichia coli
Krueger et al. Unravelling post‐transcriptional PrmC‐dependent regulatory mechanisms in Pseudomonas aeruginosa
JPWO2002097086A1 (ja) 工業的生産に有用な微生物
Negus et al. A poly‐γ‐d‐glutamic acid depolymerase that degrades the protective capsule of B acillus anthracis
Arima et al. Recombinant production and characterization of an N-acyl-D-amino acid amidohydrolase from Streptomyces sp. 64E6