EA199800342A1 - Ювенильный гормон и один из его агонистов в качестве химического лиганда для контроля экспрессии гена посредством трансактивации, опосредованной рецептором - Google Patents

Ювенильный гормон и один из его агонистов в качестве химического лиганда для контроля экспрессии гена посредством трансактивации, опосредованной рецептором

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Publication number
EA199800342A1
EA199800342A1 EA199800342A EA199800342A EA199800342A1 EA 199800342 A1 EA199800342 A1 EA 199800342A1 EA 199800342 A EA199800342 A EA 199800342A EA 199800342 A EA199800342 A EA 199800342A EA 199800342 A1 EA199800342 A1 EA 199800342A1
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EA
Eurasian Patent Office
Prior art keywords
receptor
usp
expression cassette
polypeptide
target
Prior art date
Application number
EA199800342A
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English (en)
Other versions
EA003423B1 (ru
Inventor
Лайл Дин Кросслэнд
Артур Стефен Гофф
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Новартис Аг
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Application filed by Новартис Аг filed Critical Новартис Аг
Publication of EA199800342A1 publication Critical patent/EA199800342A1/ru
Publication of EA003423B1 publication Critical patent/EA003423B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70567—Nuclear receptors, e.g. retinoic acid receptor [RAR], RXR, nuclear orphan receptors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/721—Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Endocrinology (AREA)
  • High Energy & Nuclear Physics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

В заявке описан способ регулирования экспрессии гена в растениях. В частности, способ включает трансформацию растения с помощью кассеты экспрессии рецептора USP, кодирующей полипептид рецептора USP, и по крайней мере одной кассеты экспрессии мишени, кодирующей полипептид-мишень. Контактирование трансформированного растения с ювенильным гормоном или одним из его агонистов активирует экспрессию полипептида-мишени в присутствии полипептида рецептора USP. Необязательно могут применяться дополнительные "вспомогательные" кассеты экспрессии рецептора, причем такая вспомогательная кассета экспрессии рецептора кодирует полипептид рецептора, отличный от USP. Способ пригоден для регулирования различных агрономически важных признаков таких как фертильность растений. Также описан способ выявления ранее неизвестных лигандов для USP. Подлежащие тестированию соединения выявляют путем контактирования с растительными клетками, трансформированными с помощью кассеты экспрессии рецептора USP и кассеты экспрессии мишени. Кассета экспрессии мишени кодирует репортерный полипептид, экспрессия которого может быть определена качественно или количественно, посредством чего выявляют, является ли тестируемое соединение лигандом для USP.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA199800342A 1995-10-10 1996-09-27 Штамм трансгенных растительных клеток и трансгенное растение, содержащие кассету экспрессии полипептида-мишени usp рецептора, способы получения их потомства, способы регулирования экспрессии полипептида-мишени в указанном растении с помощью ювенильного гормона или его агонистов, способы выявления и получения лиганда для полипептида usp рецептора и соответствующий лиганд EA003423B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US610895P 1995-10-10 1995-10-10
PCT/EP1996/004224 WO1997013864A1 (en) 1995-10-10 1996-09-27 Juvenile hormone or one of its agonists as a chemical ligand to control gene expression in plants by receptor mediated transactivation

Publications (2)

Publication Number Publication Date
EA199800342A1 true EA199800342A1 (ru) 1998-12-24
EA003423B1 EA003423B1 (ru) 2003-04-24

Family

ID=21719352

Family Applications (1)

Application Number Title Priority Date Filing Date
EA199800342A EA003423B1 (ru) 1995-10-10 1996-09-27 Штамм трансгенных растительных клеток и трансгенное растение, содержащие кассету экспрессии полипептида-мишени usp рецептора, способы получения их потомства, способы регулирования экспрессии полипептида-мишени в указанном растении с помощью ювенильного гормона или его агонистов, способы выявления и получения лиганда для полипептида usp рецептора и соответствующий лиганд

Country Status (14)

Country Link
US (1) US6362394B1 (ru)
EP (1) EP0859851A1 (ru)
JP (1) JP2000500323A (ru)
KR (1) KR19990064155A (ru)
CN (1) CN1202202A (ru)
AU (1) AU710122B2 (ru)
BR (1) BR9611032A (ru)
CA (1) CA2232712A1 (ru)
EA (1) EA003423B1 (ru)
HU (1) HUP9900029A3 (ru)
IL (1) IL123796A0 (ru)
PL (1) PL326223A1 (ru)
TR (1) TR199800657T1 (ru)
WO (1) WO1997013864A1 (ru)

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DE10254166A1 (de) 2002-11-20 2004-06-03 Icon Genetics Ag Verfahren zur Kontrolle eines zellulären Prozesses in Pflanzen
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WO2005022167A2 (en) * 2003-08-29 2005-03-10 The University Of North Carolina At Greensboro Compounds that act to modulate insect growth and methods and systems for identifying such compounds
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US8637237B2 (en) * 2003-08-29 2014-01-28 The University Of North Carolina At Greensboro Methods, compositions, and systems for the identification of species-specific or developmental stage-specific insecticides
JP4814686B2 (ja) * 2006-04-24 2011-11-16 岩手県 雌雄配偶子形成不全植物の作成方法
US7745698B2 (en) 2006-11-16 2010-06-29 Wisconsin Alumni Research Foundation Peptide extension for enhancement of transgene expression in chloroplasts
BRPI1008606A2 (pt) * 2009-02-13 2017-05-30 Univ California planta, célula de planta, semente, flor, folha ou fruta, método para aumentar a tolerância aos estresse em uma planta, cassete e vetor de expressão, método para preparar uma planta, formulação química agrícola, e, métodos para aumentar a tolerância ao estresse em uma planta e para identificar um agente que agoniza um polipeptídeo pyr/pyl.
CN104109673B (zh) * 2014-07-10 2017-01-11 中国农业科学院作物科学研究所 一种蜕皮激素受体基因USP dsRNA及其在控制蚜虫危害中的应用

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Also Published As

Publication number Publication date
IL123796A0 (en) 1998-10-30
KR19990064155A (ko) 1999-07-26
CA2232712A1 (en) 1997-04-17
TR199800657T1 (xx) 1998-06-22
JP2000500323A (ja) 2000-01-18
AU7215796A (en) 1997-04-30
MX9802807A (es) 1998-09-30
HUP9900029A3 (en) 2001-02-28
BR9611032A (pt) 1999-07-06
PL326223A1 (en) 1998-08-31
WO1997013864A1 (en) 1997-04-17
HUP9900029A2 (hu) 1999-04-28
US6362394B1 (en) 2002-03-26
EP0859851A1 (en) 1998-08-26
EA003423B1 (ru) 2003-04-24
CN1202202A (zh) 1998-12-16
AU710122B2 (en) 1999-09-16

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM RU