EA007652B1 - Способ обнаружения и анализа полинуклеотидов с помощью светособирающих полихромофоров - Google Patents

Способ обнаружения и анализа полинуклеотидов с помощью светособирающих полихромофоров Download PDF

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Publication number
EA007652B1
EA007652B1 EA200500041A EA200500041A EA007652B1 EA 007652 B1 EA007652 B1 EA 007652B1 EA 200500041 A EA200500041 A EA 200500041A EA 200500041 A EA200500041 A EA 200500041A EA 007652 B1 EA007652 B1 EA 007652B1
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EA
Eurasian Patent Office
Prior art keywords
sensory
target polynucleotide
pna
polychromophore
sample
Prior art date
Application number
EA200500041A
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English (en)
Russian (ru)
Other versions
EA200500041A1 (ru
Inventor
Гильермо К. Базан
Брент С. Гейлорд
Original Assignee
Дзе Риджентс Оф Дзе Юниверсити Оф Калифорния
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Publication of EA200500041A1 publication Critical patent/EA200500041A1/ru
Publication of EA007652B1 publication Critical patent/EA007652B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82B—NANOSTRUCTURES FORMED BY MANIPULATION OF INDIVIDUAL ATOMS, MOLECULES, OR LIMITED COLLECTIONS OF ATOMS OR MOLECULES AS DISCRETE UNITS; MANUFACTURE OR TREATMENT THEREOF
    • B82B3/00—Manufacture or treatment of nanostructures by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/107—Modifications characterised by incorporating a peptide nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/107—Alteration in the property of hybridised versus free label oligonucleotides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
EA200500041A 2002-06-20 2003-06-20 Способ обнаружения и анализа полинуклеотидов с помощью светособирающих полихромофоров EA007652B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US39052402P 2002-06-20 2002-06-20
US40626602P 2002-08-26 2002-08-26
PCT/US2003/019678 WO2004001379A2 (en) 2002-06-20 2003-06-20 Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores

Publications (2)

Publication Number Publication Date
EA200500041A1 EA200500041A1 (ru) 2005-08-25
EA007652B1 true EA007652B1 (ru) 2006-12-29

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Family Applications (1)

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EA200500041A EA007652B1 (ru) 2002-06-20 2003-06-20 Способ обнаружения и анализа полинуклеотидов с помощью светособирающих полихромофоров

Country Status (12)

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US (2) US7214489B2 (US07214489-20070508-C00002.png)
EP (2) EP1534857B1 (US07214489-20070508-C00002.png)
JP (1) JP4528115B2 (US07214489-20070508-C00002.png)
KR (1) KR20050010956A (US07214489-20070508-C00002.png)
CN (2) CN102766695A (US07214489-20070508-C00002.png)
AT (1) ATE476521T1 (US07214489-20070508-C00002.png)
AU (1) AU2003243722A1 (US07214489-20070508-C00002.png)
CA (1) CA2489922C (US07214489-20070508-C00002.png)
DE (1) DE60333646D1 (US07214489-20070508-C00002.png)
EA (1) EA007652B1 (US07214489-20070508-C00002.png)
IL (1) IL165877A0 (US07214489-20070508-C00002.png)
WO (1) WO2004001379A2 (US07214489-20070508-C00002.png)

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Publication number Publication date
DE60333646D1 (de) 2010-09-16
WO2004001379A3 (en) 2004-06-03
ATE476521T1 (de) 2010-08-15
AU2003243722A1 (en) 2004-01-06
KR20050010956A (ko) 2005-01-28
US20080280369A1 (en) 2008-11-13
CA2489922A1 (en) 2003-12-31
EP1534857B1 (en) 2010-08-04
EA200500041A1 (ru) 2005-08-25
US8101416B2 (en) 2012-01-24
CN102766695A (zh) 2012-11-07
EP1534857A2 (en) 2005-06-01
IL165877A0 (en) 2006-01-15
EP2278027A3 (en) 2011-02-16
JP4528115B2 (ja) 2010-08-18
CN1675377B (zh) 2012-10-03
EP2278027A2 (en) 2011-01-26
US7214489B2 (en) 2007-05-08
US20040219556A1 (en) 2004-11-04
CN1675377A (zh) 2005-09-28
WO2004001379A2 (en) 2003-12-31
JP2005530182A (ja) 2005-10-06
EP1534857A4 (en) 2007-10-31
CA2489922C (en) 2016-08-16

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