DK3660146T3 - Ydelsesforbedrede og opbevaringsstabile proteasevarianter - Google Patents

Ydelsesforbedrede og opbevaringsstabile proteasevarianter Download PDF

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Publication number
DK3660146T3
DK3660146T3 DK18209177.7T DK18209177T DK3660146T3 DK 3660146 T3 DK3660146 T3 DK 3660146T3 DK 18209177 T DK18209177 T DK 18209177T DK 3660146 T3 DK3660146 T3 DK 3660146T3
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DK
Denmark
Prior art keywords
enhanced
performance
storage
protease variants
stable protease
Prior art date
Application number
DK18209177.7T
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English (en)
Other versions
DK3660146T5 (da
Inventor
Layla Fernandez
Sabine Griemert
Nina Mussmann
Inken Prueser
Daria Strauss
Christian Degering
Susanne Wieland
Original Assignee
Henkel Ag & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=64559554&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK3660146(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Henkel Ag & Co Kgaa filed Critical Henkel Ag & Co Kgaa
Application granted granted Critical
Publication of DK3660146T3 publication Critical patent/DK3660146T3/da
Publication of DK3660146T5 publication Critical patent/DK3660146T5/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1024In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21062Subtilisin (3.4.21.62)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DK18209177.7T 2018-11-29 2018-11-29 Ydelsesforbedrede og opbevaringsstabile proteasevarianter DK3660146T5 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP18209177.7A EP3660146B1 (de) 2018-11-29 2018-11-29 Leistungsverbesserte und lagerstabile protease-varianten

Publications (2)

Publication Number Publication Date
DK3660146T3 true DK3660146T3 (da) 2024-02-19
DK3660146T5 DK3660146T5 (da) 2024-08-26

Family

ID=64559554

Family Applications (1)

Application Number Title Priority Date Filing Date
DK18209177.7T DK3660146T5 (da) 2018-11-29 2018-11-29 Ydelsesforbedrede og opbevaringsstabile proteasevarianter

Country Status (5)

Country Link
US (1) US11746341B2 (da)
EP (1) EP3660146B1 (da)
DK (1) DK3660146T5 (da)
ES (1) ES2967424T3 (da)
PL (1) PL3660146T3 (da)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102020205381A1 (de) * 2020-04-29 2021-11-04 Henkel Ag & Co. Kgaa Hochalkalisches Textilwaschmittel mit Protease
EP4204547A1 (en) * 2020-08-28 2023-07-05 Novozymes A/S Protease variants with improved solubility
CA3257053A1 (en) 2022-05-14 2023-11-23 Novozymes A/S COMPOSITIONS AND METHODS FOR THE PREVENTION, TREATMENT, SUPPRESSION AND/OR ELIMINATION OF PHYTOPATHOGENIC INFESTATIONS AND INFECTIONS
DE102022205588A1 (de) * 2022-06-01 2023-12-07 Henkel Ag & Co. Kgaa Wasch- und reinigungsmittel mit verbesserter enzymstabilität
WO2024094735A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions
CN120693402A (zh) 2022-11-04 2025-09-23 巴斯夫欧洲公司 用于在洗涤剂组合物中使用的具有蛋白酶活性的多肽
WO2024094733A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202369A1 (en) * 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
EP4624572A1 (en) 2024-03-27 2025-10-01 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202382A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in cleaning compositions
WO2025202379A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202370A1 (en) * 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202372A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202374A1 (en) * 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
EP1921147B1 (en) 1994-02-24 2011-06-08 Henkel AG & Co. KGaA Improved enzymes and detergents containing them
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DE102004027091A1 (de) * 2004-06-02 2005-12-29 Henkel Kgaa Leistungsverbesserte Alkalische Protease-Varianten und Wasch- und Reinigungsmittel, enthaltend diese leistungsverbesserten Alkalische Protease-Varianten
DE102008017103A1 (de) 2008-04-02 2009-10-08 Henkel Ag & Co. Kgaa Wasch- und Reinigungsmittel enthaltend Proteasen aus Xanthomonas
DE102009029513A1 (de) 2009-09-16 2011-03-24 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Proteasen
DE102010028951A1 (de) 2010-05-12 2011-11-17 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Lipase
DE102010063458A1 (de) 2010-12-17 2012-06-21 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Amylase
DE102011118021A1 (de) * 2011-10-28 2013-05-02 Henkel Ag & Co. Kgaa Leistungsverbesserte und temperaturstabile Proteasevarianten
DE102012201522A1 (de) * 2012-02-02 2013-08-08 Basf Se Lagerstabiles flüssiges Geschirrspülmittel enthaltend Protease und Amylase
DE102013218253A1 (de) 2013-09-12 2015-03-12 Henkel Ag & Co. Kgaa Festes Textilwaschmittel mit verbesserter Proteaseleistung
DE102013219467A1 (de) 2013-09-26 2015-03-26 Henkel Ag & Co. Kgaa Stabilisierte Inhibitor-Proteasevarianten
DE102013221206A1 (de) 2013-10-18 2015-04-23 Henkel Ag & Co. Kgaa Proteasevarianten mit erhöhter Stabilität
EP3122762A1 (en) * 2014-03-28 2017-02-01 Novozymes A/S Enzyme variants and polynucleotides encoding same
EP3152290A1 (en) * 2014-06-04 2017-04-12 Novozymes A/S Detergent composition
EP3878960A1 (en) 2014-07-04 2021-09-15 Novozymes A/S Subtilase variants and polynucleotides encoding same
DK3212778T3 (da) * 2014-10-28 2019-11-04 Agrivida Inc Fremgangsmåder og sammensætninger til stabilisering af trans-splejsning af intein-modificerede proteaser
DK3399031T3 (da) 2014-12-15 2020-01-20 Henkel Ag & Co Kgaa Detergentsammensætning med subtilasevarianter
DK3234093T3 (da) 2014-12-19 2020-08-17 Novozymes As Proteasevarianter og polynukleotider, som koder for dem
DE102015208655A1 (de) 2015-05-11 2016-11-17 Henkel Ag & Co. Kgaa Enzymstabilisatoren
CA3027272C (en) * 2016-07-13 2022-06-21 The Procter & Gamble Company Bacillus cibi dnase variants and uses thereof
WO2018069158A1 (en) * 2016-10-11 2018-04-19 Basf Se Protease resistant to natural inhibitors
EP3309244A1 (en) 2016-10-11 2018-04-18 Basf Se Low temperature protease
US20190292494A1 (en) 2016-12-01 2019-09-26 Basf Se Stabilization of enzymes in compositions
WO2018161899A1 (en) 2017-03-06 2018-09-13 Novozymes A/S Use of one or more enzymes in preventing, inhibiting or reducing microbe growth on a surface

Also Published As

Publication number Publication date
PL3660146T3 (pl) 2024-03-18
ES2967424T3 (es) 2024-04-30
US11746341B2 (en) 2023-09-05
EP3660146A1 (de) 2020-06-03
EP3660146B1 (de) 2023-11-15
DK3660146T5 (da) 2024-08-26
US20200172890A1 (en) 2020-06-04

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