DK3529611T3 - Fremgangsmåder til screening af b-cellelymfocytter - Google Patents

Fremgangsmåder til screening af b-cellelymfocytter Download PDF

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Publication number
DK3529611T3
DK3529611T3 DK17862641.2T DK17862641T DK3529611T3 DK 3529611 T3 DK3529611 T3 DK 3529611T3 DK 17862641 T DK17862641 T DK 17862641T DK 3529611 T3 DK3529611 T3 DK 3529611T3
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Denmark
Prior art keywords
screening
methods
cell lymphocytes
lymphocytes
cell
Prior art date
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DK17862641.2T
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English (en)
Inventor
Minha Park
Jason C Briggs
Jason M Mcewen
Ravi K Ramenani
Dinakar Hariharasudhan Chirra
Kai W Szeto
Adrienne T Higa
Mark P White
Randall D Lowe Jr
Xiaohua Wang
Kevin T Chapman
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Berkeley Lights Inc
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Publication of DK3529611T3 publication Critical patent/DK3529611T3/da

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
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DK17862641.2T 2016-10-23 2017-10-23 Fremgangsmåder til screening af b-cellelymfocytter DK3529611T3 (da)

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US201662411690P 2016-10-23 2016-10-23
US201662412092P 2016-10-24 2016-10-24
PCT/US2017/057926 WO2018076024A2 (en) 2016-10-23 2017-10-23 Methods for screening b cell lymphocytes

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DK3529611T3 true DK3529611T3 (da) 2023-02-06

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US (1) US20200064337A1 (da)
EP (2) EP3529611B1 (da)
JP (2) JP7053601B2 (da)
KR (2) KR102560201B1 (da)
CN (2) CN114755412A (da)
AU (1) AU2017345813A1 (da)
CA (1) CA3041527C (da)
DK (1) DK3529611T3 (da)
IL (1) IL266208B1 (da)
SG (1) SG11201903557SA (da)
WO (1) WO2018076024A2 (da)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018111765A1 (en) 2016-12-12 2018-06-21 xCella Biosciences, Inc. Methods and systems for screening using microcapillary arrays
KR20200026878A (ko) 2017-06-06 2020-03-11 지머젠 인코포레이티드 균류 균주를 개량하기 위한 htp 게놈 공학 플랫폼
KR20210018219A (ko) 2018-06-06 2021-02-17 지머젠 인코포레이티드 발효 및 생산 중 곰팡이 형태를 제어하기 위한 신호 전달에 포함된 유전자의 조작
SG11202103348TA (en) * 2018-10-11 2021-04-29 Berkeley Lights Inc Systems and methods for identification of optimized protein production and kits therefor
JPWO2021065766A1 (da) * 2019-09-30 2021-04-08
TW202142856A (zh) 2019-11-17 2021-11-16 美商伯克利之光生命科技公司 用於生物樣本之分析的系統及方法
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
AU2021409785A1 (en) * 2020-12-22 2023-07-06 Amgen Inc. Diffusion gradient assay for anti-cd3-containing molecules
EP4239333A1 (en) * 2022-03-03 2023-09-06 Lonza Biologics plc Method for selecting clonal cells expressing correctly assembled multi-specific binding molecules

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3696289A (en) * 1988-06-08 1990-01-05 Cambridge Bioscience Corporation Anti-fc assay for detection of antibodies
JP2618497B2 (ja) * 1989-10-03 1997-06-11 鐘淵化学工業株式会社 腫瘍障害細胞誘導剤および腫瘍障害細胞誘導デバイス
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US6942776B2 (en) 1999-05-18 2005-09-13 Silicon Biosystems S.R.L. Method and apparatus for the manipulation of particles by means of dielectrophoresis
ATE500051T1 (de) * 2001-04-06 2011-03-15 Fluidigm Corp Polymeroberflächenmodifikation
US20060073095A1 (en) * 2001-08-22 2006-04-06 Steven Kessler Methods for screening antibody-producing cells on heterogeneous antigen substrates
US6958132B2 (en) 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
CA2496741A1 (en) * 2002-08-28 2004-04-29 Bionexus Ventures L.L.C. Screening compound libraries using an optical fiber array device capable of simultaneously performing multiple functional assays
CA2505924A1 (en) * 2002-11-13 2004-05-27 Micromet Ag Method for identifying antigen specific b cells
US20050014230A1 (en) * 2003-07-16 2005-01-20 Ccl Holding Co., Ltd. Preparation of fully human antibodies
WO2005100541A2 (en) 2004-04-12 2005-10-27 The Regents Of The University Of California Optoelectronic tweezers for microparticle and cell manipulation
CN100378122C (zh) * 2004-12-03 2008-04-02 中国人民解放军第三军医大学 B淋巴细胞刺激因子抑制肽及其制备方法
US7488596B2 (en) * 2004-12-17 2009-02-10 Samsung Electronics Co., Ltd. Microfluidic device comprising electrolysis device for cell lysis and method for electrochemically lysing cells using the same
US20070196820A1 (en) * 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
WO2007016366A2 (en) * 2005-07-29 2007-02-08 Yale University Defined culture conditions of human embryonic stem cells
US8080517B2 (en) * 2005-09-12 2011-12-20 Xigen Sa Cell-permeable peptide inhibitors of the JNK signal transduction pathway
WO2007092713A2 (en) * 2006-02-02 2007-08-16 Trustees Of The University Of Pennsylvania Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease
US8685344B2 (en) 2007-01-22 2014-04-01 Advanced Liquid Logic, Inc. Surface assisted fluid loading and droplet dispensing
WO2008119066A1 (en) 2007-03-28 2008-10-02 The Regents Of The University Of California Single-sided lateral-field and phototransistor-based optoelectronic tweezers
EP2185718B1 (en) * 2007-08-03 2017-10-04 MUSC Foundation For Research Development Human monoclonal antibodies and methods for producing the same
US20090098594A1 (en) * 2007-08-21 2009-04-16 Nodality, Inc. Methods for diagnosis prognosis and methods of treatment
WO2009151505A1 (en) * 2008-04-11 2009-12-17 China Molecular, Ltd. Devices and methods for immunoglobulin production
JP2010035472A (ja) * 2008-08-04 2010-02-18 Shizuoka Prefecture Ｓｓｔ−ｒｅｘ法を用いた新規のがん抗原特異的抗体遺伝子スクリーニング方法
GB0818609D0 (en) * 2008-10-10 2008-11-19 Univ Hull apparatus and method
JP2010263825A (ja) * 2009-05-14 2010-11-25 Kyoto Univ 記憶ｂ細胞の培養方法
US20100317539A1 (en) * 2009-06-12 2010-12-16 Guo-Liang Yu Library of Engineered-Antibody Producing Cells
US9533306B2 (en) 2010-08-02 2017-01-03 The Regents Of The University Of California Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns
US9227200B2 (en) 2011-06-03 2016-01-05 The Regents Of The University Of California Microfluidic devices with flexible optically transparent electrodes
EP2780711B1 (en) * 2011-11-15 2016-01-06 Erasmus University Medical Center Rotterdam Methods and kits for detecting ige-expressing b cells
US9403172B2 (en) 2012-11-08 2016-08-02 Berkeley Lights, Inc. Circuit based optoelectronic tweezers
EP2971039B1 (en) * 2013-03-14 2020-01-01 Immusoft Corporation Methods for in vitro memory b cell differentiation and transduction with vsv-g pseudotyped viral vectors
US9816088B2 (en) * 2013-03-15 2017-11-14 Abvitro Llc Single cell bar-coding for antibody discovery
US9889445B2 (en) * 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
CN105849561B (zh) * 2013-10-22 2018-09-14 伯克利之光生命科技公司 用于测定生物活性的微流体装置
US10010882B2 (en) 2013-10-22 2018-07-03 Berkeley Lights, Inc. Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US20150306598A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. DEP Force Control And Electrowetting Control In Different Sections Of The Same Microfluidic Apparatus
SG11201608499XA (en) 2014-04-25 2016-11-29 Berkeley Lights Inc Providing dep manipulation devices and controllable electrowetting devices in the same microfluidic apparatus
US20150306599A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. Providing DEP Manipulation Devices And Controllable Electrowetting Devices In The Same Microfluidic Apparatus
CN107206377B (zh) * 2014-12-09 2020-02-11 伯克利之光生命科技公司 微流体装置中测定阳性的区域的自动检测
WO2016094715A2 (en) * 2014-12-10 2016-06-16 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
GB201501907D0 (en) * 2015-02-05 2015-03-25 Technion Res & Dev Foundation System and method for single cell genetic analysis
JP7051206B2 (ja) * 2015-04-22 2022-04-11 バークレー ライツ，インコーポレイテッド マイクロ流体細胞培養
CN105214742B (zh) * 2015-10-10 2017-10-31 中国科学院深圳先进技术研究院 基于人工结构声场的微流体系统及操控微粒的方法

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US20200064337A1 (en) 2020-02-27
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