DK3452602T3 - Fremgangsmåde til fremstillingen af en kartoffel (solanum tubersum) hvor nævnte fremgangsmåde involverer homolog-rettet mutagenese ved anvendelse af crispr/cas9-teknik - Google Patents

Fremgangsmåde til fremstillingen af en kartoffel (solanum tubersum) hvor nævnte fremgangsmåde involverer homolog-rettet mutagenese ved anvendelse af crispr/cas9-teknik

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Publication number
DK3452602T3
DK3452602T3 DK17792956.9T DK17792956T DK3452602T3 DK 3452602 T3 DK3452602 T3 DK 3452602T3 DK 17792956 T DK17792956 T DK 17792956T DK 3452602 T3 DK3452602 T3 DK 3452602T3
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Denmark
Prior art keywords
crispr
homologue
potato
production
directed mutagenesis
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DK17792956.9T
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English (en)
Inventor
Mariette Andersson
Mathias Samuelsson
Per Hofvander
Åke Ståhl
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Sveriges Staerkelseproducenter Foerening U P A
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Publication of DK3452602T3 publication Critical patent/DK3452602T3/da

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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
    • C08B30/12—Degraded, destructured or non-chemically modified starch, e.g. mechanically, enzymatically or by irradiation; Bleaching of starch
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
    • C08B30/20—Amylose or amylopectin
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L3/00—Compositions of starch, amylose or amylopectin or of their derivatives or degradation products
    • C08L3/02—Starch; Degradation products thereof, e.g. dextrin
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L3/00—Compositions of starch, amylose or amylopectin or of their derivatives or degradation products
    • C08L3/12—Amylose; Amylopectin; Degradation products thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/80—Saxifragaceae, e.g. Heuchera

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Materials Engineering (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
DK17792956.9T 2016-05-03 2017-05-03 Fremgangsmåde til fremstillingen af en kartoffel (solanum tubersum) hvor nævnte fremgangsmåde involverer homolog-rettet mutagenese ved anvendelse af crispr/cas9-teknik DK3452602T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE1650598A SE1650598A1 (en) 2016-05-03 2016-05-03 Amylopectin potato starch with improved stability against retrogradation and improved freeze and thaw stability
PCT/SE2017/050429 WO2017192095A1 (en) 2016-05-03 2017-05-03 Amylopectin potato starch with improved stability against retrogradation and improved freeze and thaw stability

Publications (1)

Publication Number Publication Date
DK3452602T3 true DK3452602T3 (da) 2025-07-07

Family

ID=60203128

Family Applications (1)

Application Number Title Priority Date Filing Date
DK17792956.9T DK3452602T3 (da) 2016-05-03 2017-05-03 Fremgangsmåde til fremstillingen af en kartoffel (solanum tubersum) hvor nævnte fremgangsmåde involverer homolog-rettet mutagenese ved anvendelse af crispr/cas9-teknik

Country Status (8)

Country Link
US (1) US11905343B2 (da)
EP (1) EP3452602B1 (da)
CN (1) CN109312358A (da)
DK (1) DK3452602T3 (da)
FI (1) FI3452602T3 (da)
PL (1) PL3452602T3 (da)
SE (1) SE1650598A1 (da)
WO (1) WO2017192095A1 (da)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE1650598A1 (en) 2016-05-03 2017-11-04 Lyckeby Starch Ab Amylopectin potato starch with improved stability against retrogradation and improved freeze and thaw stability
WO2019145544A1 (en) 2018-01-26 2019-08-01 Böhm-Nordkartoffel Agrarproduktion Gmbh & Co. Ohg Altered starch producing plants
CN109852635A (zh) * 2019-03-01 2019-06-07 安徽华明太合生物工程有限公司 一种快速检测基因编辑有效性的方法
BR112023024759A2 (pt) * 2021-05-28 2024-02-15 Cooeperatie Koninklijke Avebe U A Amido de amilopectina para ligação de produtos de carne
CN113447626A (zh) * 2021-06-10 2021-09-28 浙江大学 一种评价山药糯性的方法
CN113999850B (zh) * 2021-11-23 2023-06-23 云南农业大学 马铃薯u6 rna聚合酶iii型启动子及其克隆与应用
CN114836564A (zh) * 2022-05-09 2022-08-02 山西农业大学 一种针对糜子gbssi基因开发的功能标记
CN116200518B (zh) * 2022-09-20 2023-10-27 宁夏农林科学院农业生物技术研究中心（宁夏农业生物技术重点实验室） 一种与马铃薯淀粉含量相关的kasp标记的开发及应用
CN118109448B (zh) * 2024-03-18 2024-12-06 武汉新华扬生物股份有限公司 一种提高核酸酶稳定性的助剂及其制备方法和应用
CN118325860B (zh) * 2024-05-21 2024-11-01 内蒙古工业大学 一种马铃薯淀粉合成酶

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SE467358B (sv) * 1990-12-21 1992-07-06 Amylogene Hb Genteknisk foeraendring av potatis foer bildning av staerkelse av amylopektintyp
US6600093B1 (en) * 1992-02-14 2003-07-29 Cooperatieve Verkoop-En Productievereniging Van Aardappelmeel En Derivaten Avebe B.A. Potato plant producing essentially amylose-free starch
DE4441408A1 (de) 1994-11-10 1996-05-15 Inst Genbiologische Forschung DNA-Sequenzen aus Solanum tuberosum kodierend Enzyme, die an der Stärkesynthese beteiligt sind, Plasmide, Bakterien, Pflanzenzellen und transgene Pflanzen enhaltend diese Sequenzen
GB9525353D0 (en) 1995-12-12 1996-02-14 Nat Starch Chem Invest Potato soluble starch synthase
JP2002518015A (ja) 1998-06-15 2002-06-25 ナショナル スターチ アンド ケミカル インベストメント ホールディング コーポレイション 植物および植物製品の改良またはそれらに関する改良
ATE255169T1 (de) * 1998-07-22 2003-12-15 Avebe Coop Verkoop Prod Enthaarungsmittel enthaltend verdicker
DE19836098A1 (de) 1998-07-31 2000-02-03 Hoechst Schering Agrevo Gmbh Pflanzen, die eine modifizierte Stärke synthetisieren, Verfahren zur Herstellung der Pflanzen, ihre Verwendung sowie die modifizierte Stärke
AUPQ005299A0 (en) 1999-04-29 1999-05-27 Commonwealth Scientific And Industrial Research Organisation Novel genes encoding wheat starch synthases and uses therefor
DE19937643A1 (de) 1999-08-12 2001-02-22 Aventis Cropscience Gmbh Transgene Zellen und Pflanzen mit veränderter Aktivität des GBSSI- und des BE-Proteins
GB9921830D0 (en) 1999-09-15 1999-11-17 Nat Starch Chem Invest Plants having reduced activity in two or more starch-modifying enzymes
CN100509853C (zh) * 2001-10-17 2009-07-08 巴斯福种植科学有限公司 淀粉
DE102004029763A1 (de) * 2004-06-21 2006-01-05 Bayer Cropscience Gmbh Pflanzen, die Amylopektin-Stärke mit neuen Eigenschaften herstellen
EP1707632A1 (de) * 2005-04-01 2006-10-04 Bayer CropScience GmbH Phosphorylierte waxy-Kartoffelstärke
EP1950303A1 (de) 2007-01-26 2008-07-30 Bayer CropScience AG Genetisch modifizierte Pflanzen, die eine Stärke mit geringem Amylosegehalt und erhöhtem Quellvermögen synthetisieren
EP2319872A1 (en) * 2009-11-04 2011-05-11 BASF Plant Science GmbH Amylopectin type starch with enhanced retrogradation stability
US20150067922A1 (en) 2013-05-30 2015-03-05 The Penn State Research Foundation Gene targeting and genetic modification of plants via rna-guided genome editing
CA2952906A1 (en) 2014-06-20 2015-12-23 Cellectis Potatoes with reduced granule-bound starch synthase
SE1650598A1 (en) 2016-05-03 2017-11-04 Lyckeby Starch Ab Amylopectin potato starch with improved stability against retrogradation and improved freeze and thaw stability

Also Published As

Publication number Publication date
PL3452602T3 (pl) 2025-09-22
FI3452602T3 (fi) 2025-06-25
US20190135946A1 (en) 2019-05-09
WO2017192095A1 (en) 2017-11-09
SE1650598A1 (en) 2017-11-04
EP3452602A4 (en) 2020-03-25
US11905343B2 (en) 2024-02-20
EP3452602B1 (en) 2025-04-02
EP3452602A1 (en) 2019-03-13
CN109312358A (zh) 2019-02-05

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