DK3377632T3 - Epitop-tag og fremgangsmåde til påvisning, indfangning og/eller oprensning af taggede polypeptider - Google Patents

Epitop-tag og fremgangsmåde til påvisning, indfangning og/eller oprensning af taggede polypeptider Download PDF

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Publication number
DK3377632T3
DK3377632T3 DK16797856.8T DK16797856T DK3377632T3 DK 3377632 T3 DK3377632 T3 DK 3377632T3 DK 16797856 T DK16797856 T DK 16797856T DK 3377632 T3 DK3377632 T3 DK 3377632T3
Authority
DK
Denmark
Prior art keywords
epitop
catching
tags
cleaning
procedure
Prior art date
Application number
DK16797856.8T
Other languages
English (en)
Inventor
Ulrich Rothbauer
Oliver Poetz
Tina Romer
Andrea Buchfellner
Larisa Yurlova
Kourosh Zolghadr
Jaqueline Bogner
Benjamin Ruf
Christian Linke-Winnebeck
Michael Metterlein
Original Assignee
ChromoTek GmbH
Nmi Naturwissenschaftliches Und Medizinisches Institut An Der Univ Tuebingen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ChromoTek GmbH, Nmi Naturwissenschaftliches Und Medizinisches Institut An Der Univ Tuebingen filed Critical ChromoTek GmbH
Application granted granted Critical
Publication of DK3377632T3 publication Critical patent/DK3377632T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/567—Framework region [FR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
DK16797856.8T 2015-11-16 2016-11-15 Epitop-tag og fremgangsmåde til påvisning, indfangning og/eller oprensning af taggede polypeptider DK3377632T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15194838.7A EP3168306A1 (en) 2015-11-16 2015-11-16 Epitope tag and method for detection and/or purification of tagged polypeptides
PCT/EP2016/077785 WO2017085086A1 (en) 2015-11-16 2016-11-15 Epitope tag and method for detection, capture and/or purification of tagged polypeptides

Publications (1)

Publication Number Publication Date
DK3377632T3 true DK3377632T3 (da) 2020-05-11

Family

ID=54608331

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16797856.8T DK3377632T3 (da) 2015-11-16 2016-11-15 Epitop-tag og fremgangsmåde til påvisning, indfangning og/eller oprensning af taggede polypeptider

Country Status (9)

Country Link
US (3) US10125166B2 (da)
EP (2) EP3168306A1 (da)
JP (1) JP6967246B2 (da)
CN (1) CN108291229A (da)
CA (1) CA3004671C (da)
DK (1) DK3377632T3 (da)
ES (1) ES2800069T3 (da)
PL (1) PL3377632T3 (da)
WO (1) WO2017085086A1 (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3168306A1 (en) 2015-11-16 2017-05-17 ChromoTek GmbH Epitope tag and method for detection and/or purification of tagged polypeptides
JP2022500076A (ja) 2018-09-11 2022-01-04 ナノタグ バイオテクノロジーズ ゲーエムベーハー 特異的結合剤により認識されるエピトープタグ
JP2023506961A (ja) 2019-12-20 2023-02-20 フエー・イー・ベー・フエー・ゼツト・ウエー ナノボディー交換クロマトグラフィー
EP4141447A4 (en) * 2020-04-24 2023-10-25 Panasonic Intellectual Property Management Co., Ltd. DETECTION METHOD, DETECTION DEVICE AND DIELECTRIC PARTICLES
WO2023222580A1 (en) * 2022-05-16 2023-11-23 Byondis B.V. Novel masked antibodies

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2427257C (en) 2000-11-01 2014-01-21 The Government Of The United States Of America Expression vectors able to elicit improved immune response and methods of using same
WO2006010106A2 (en) 2004-07-09 2006-01-26 The Government Of The United States, As Represented By The Secretary Of The Department Of Health And Human Services Dna-based vaccination of retroviral-infected individuals undergoing treatment
GB201008682D0 (en) * 2010-05-25 2010-07-07 Vib Vzw Epitope tag for affinity based applications
WO2014014207A1 (en) * 2012-07-20 2014-01-23 University-Industry Cooperation Group Of Kyung Hee University Antibody for epitope tagging, hybridoma cell line and uses thereof
EP3168306A1 (en) 2015-11-16 2017-05-17 ChromoTek GmbH Epitope tag and method for detection and/or purification of tagged polypeptides

Also Published As

Publication number Publication date
CA3004671A1 (en) 2017-05-26
US10273265B2 (en) 2019-04-30
JP6967246B2 (ja) 2021-11-17
EP3377632A1 (en) 2018-09-26
WO2017085086A1 (en) 2017-05-26
US20170137467A1 (en) 2017-05-18
ES2800069T3 (es) 2020-12-23
CA3004671C (en) 2022-10-18
US11098081B2 (en) 2021-08-24
US20200040033A1 (en) 2020-02-06
US10125166B2 (en) 2018-11-13
EP3168306A1 (en) 2017-05-17
CN108291229A (zh) 2018-07-17
US20170137464A1 (en) 2017-05-18
PL3377632T3 (pl) 2020-08-24
EP3377632B1 (en) 2020-04-15
JP2019504637A (ja) 2019-02-21

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