PL3377632T3 - Znacznik epitopowy i sposób wykrywania, wychwytywania i/lub oczyszczania znakowanych polipeptydów - Google Patents

Znacznik epitopowy i sposób wykrywania, wychwytywania i/lub oczyszczania znakowanych polipeptydów

Info

Publication number
PL3377632T3
PL3377632T3 PL16797856T PL16797856T PL3377632T3 PL 3377632 T3 PL3377632 T3 PL 3377632T3 PL 16797856 T PL16797856 T PL 16797856T PL 16797856 T PL16797856 T PL 16797856T PL 3377632 T3 PL3377632 T3 PL 3377632T3
Authority
PL
Poland
Prior art keywords
capture
purification
detection
epitope tag
tagged polypeptides
Prior art date
Application number
PL16797856T
Other languages
English (en)
Inventor
Ulrich Rothbauer
Oliver Poetz
Tina Romer
Andrea BUCHFELLNER
Larisa YURLOVA
Kourosh Zolghadr
Jaqueline BOGNER
Benjamin RUF
Christian Linke-Winnebeck
Michael Metterlein
Original Assignee
ChromoTek GmbH
NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ChromoTek GmbH, NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen filed Critical ChromoTek GmbH
Publication of PL3377632T3 publication Critical patent/PL3377632T3/pl

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K7/00Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04Linear peptides containing only normal peptide links
    • C07K7/06Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/22Affinity chromatography or related techniques based upon selective absorption processes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K7/00Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04Linear peptides containing only normal peptide links
    • C07K7/08Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/30Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/34Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565Complementarity determining region [CDR]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/567Framework region [FR]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/90Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/40Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
PL16797856T 2015-11-16 2016-11-15 Znacznik epitopowy i sposób wykrywania, wychwytywania i/lub oczyszczania znakowanych polipeptydów PL3377632T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP15194838.7A EP3168306A1 (en) 2015-11-16 2015-11-16 Epitope tag and method for detection and/or purification of tagged polypeptides
EP16797856.8A EP3377632B1 (en) 2015-11-16 2016-11-15 Epitope tag and method for detection, capture and/or purification of tagged polypeptides
PCT/EP2016/077785 WO2017085086A1 (en) 2015-11-16 2016-11-15 Epitope tag and method for detection, capture and/or purification of tagged polypeptides

Publications (1)

Publication Number Publication Date
PL3377632T3 true PL3377632T3 (pl) 2020-08-24

Family

ID=54608331

Family Applications (1)

Application Number Title Priority Date Filing Date
PL16797856T PL3377632T3 (pl) 2015-11-16 2016-11-15 Znacznik epitopowy i sposób wykrywania, wychwytywania i/lub oczyszczania znakowanych polipeptydów

Country Status (9)

Country Link
US (3) US10125166B2 (pl)
EP (2) EP3168306A1 (pl)
JP (1) JP6967246B2 (pl)
CN (1) CN108291229A (pl)
CA (1) CA3004671C (pl)
DK (1) DK3377632T3 (pl)
ES (1) ES2800069T3 (pl)
PL (1) PL3377632T3 (pl)
WO (1) WO2017085086A1 (pl)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3168306A1 (en) * 2015-11-16 2017-05-17 ChromoTek GmbH Epitope tag and method for detection and/or purification of tagged polypeptides
US20220048947A1 (en) 2018-09-11 2022-02-17 Nanotag Biotechnologies Gmbh Epitope tags recognized by specific binders
WO2021123360A1 (en) 2019-12-20 2021-06-24 Vib Vzw Nanobody exchange chromatography
JPWO2021215194A1 (pl) * 2020-04-24 2021-10-28
WO2023222580A1 (en) * 2022-05-16 2023-11-23 Byondis B.V. Novel masked antibodies

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2427257C (en) 2000-11-01 2014-01-21 The Government Of The United States Of America Expression vectors able to elicit improved immune response and methods of using same
WO2006010106A2 (en) 2004-07-09 2006-01-26 The Government Of The United States, As Represented By The Secretary Of The Department Of Health And Human Services Dna-based vaccination of retroviral-infected individuals undergoing treatment
GB201008682D0 (en) * 2010-05-25 2010-07-07 Vib Vzw Epitope tag for affinity based applications
WO2014014207A1 (en) * 2012-07-20 2014-01-23 University-Industry Cooperation Group Of Kyung Hee University Antibody for epitope tagging, hybridoma cell line and uses thereof
EP3168306A1 (en) 2015-11-16 2017-05-17 ChromoTek GmbH Epitope tag and method for detection and/or purification of tagged polypeptides

Also Published As

Publication number Publication date
WO2017085086A1 (en) 2017-05-26
JP2019504637A (ja) 2019-02-21
US11098081B2 (en) 2021-08-24
EP3377632B1 (en) 2020-04-15
ES2800069T3 (es) 2020-12-23
CA3004671C (en) 2022-10-18
CN108291229A (zh) 2018-07-17
US10125166B2 (en) 2018-11-13
EP3168306A1 (en) 2017-05-17
US20200040033A1 (en) 2020-02-06
EP3377632A1 (en) 2018-09-26
US20170137467A1 (en) 2017-05-18
JP6967246B2 (ja) 2021-11-17
CA3004671A1 (en) 2017-05-26
US10273265B2 (en) 2019-04-30
DK3377632T3 (da) 2020-05-11
US20170137464A1 (en) 2017-05-18

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