DK3207373T3 - Fremgangsmåde til analyse af indholdet af dråber og tilhørende indretning - Google Patents

Fremgangsmåde til analyse af indholdet af dråber og tilhørende indretning Download PDF

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Publication number
DK3207373T3
DK3207373T3 DK15785075.1T DK15785075T DK3207373T3 DK 3207373 T3 DK3207373 T3 DK 3207373T3 DK 15785075 T DK15785075 T DK 15785075T DK 3207373 T3 DK3207373 T3 DK 3207373T3
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DK
Denmark
Prior art keywords
droplets
analyzing
contents
associated device
Prior art date
Application number
DK15785075.1T
Other languages
English (en)
Inventor
Clément Nizak
Philippe Chi-Thanh Nghe
Jean Marie Pierre Baudry
Elodie Michèle Christine Brient-Litzler
Andrew David Griffiths
Alexei Godina
Raphael Clément Li-Ming Doineau
Original Assignee
Ecole Superieure Physique & Chimie Ind Ville De Paris
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Publication of DK3207373T3 publication Critical patent/DK3207373T3/da

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6846Common amplification features
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668Trapping microscopic beads
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0673Handling of plugs of fluid surrounded by immiscible fluid
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0816Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0023Investigating dispersion of liquids
    • G01N2015/003Investigating dispersion of liquids in liquids, e.g. emulsion
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0092Monitoring flocculation or agglomeration

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Dispersion Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Fluid Mechanics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DK15785075.1T 2014-10-15 2015-10-15 Fremgangsmåde til analyse af indholdet af dråber og tilhørende indretning DK3207373T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1459903A FR3027396B1 (fr) 2014-10-15 2014-10-15 Procede d'analyse du contenu de gouttes et appareil associe
PCT/EP2015/073942 WO2016059182A1 (fr) 2014-10-15 2015-10-15 Procédé d'analyse du contenu de gouttes et appareil associé

Publications (1)

Publication Number Publication Date
DK3207373T3 true DK3207373T3 (da) 2019-06-03

Family

ID=52684324

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15785075.1T DK3207373T3 (da) 2014-10-15 2015-10-15 Fremgangsmåde til analyse af indholdet af dråber og tilhørende indretning

Country Status (7)

Country Link
US (1) US10416168B2 (da)
EP (1) EP3207373B1 (da)
JP (2) JP6775498B2 (da)
CN (1) CN107110854B (da)
DK (1) DK3207373T3 (da)
FR (1) FR3027396B1 (da)
WO (1) WO2016059182A1 (da)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2984045C (en) 2015-04-30 2024-01-09 European Molecular Biology Laboratory Microfluidic droplet detection and sorting
FR3050212B1 (fr) * 2016-04-15 2020-09-25 Chu Montpellier Procede de detection et/ou de caracterisation de cellules tumorales et appareil associe
KR102009557B1 (ko) * 2016-09-21 2019-08-09 고려대학교 산학협력단 미세조류 균주 선별용 마이크로 장치
EP3351926A1 (en) * 2017-01-18 2018-07-25 Hifibio Method for analyzing and selecting a specific droplet among a plurality of droplets and associated apparatus
EP3375889B1 (en) 2017-03-17 2019-12-11 HiFiBiO SAS Single cell analysis
DE102018200520A1 (de) 2018-01-15 2019-07-18 Robert Bosch Gmbh Verfahren zum Bereitstellen einer Lösung der Substanz in einer mikrofluidischen Vorrichtung
US11686664B2 (en) * 2018-01-29 2023-06-27 Hewlett-Packard Development Company, L.P. Particle categorization
EP3543351B1 (en) 2018-03-19 2022-08-10 Ricoh Company, Ltd. Nucleic acid sample-contained container, method for producing nucleic acid sample-contained container, and nucleic acid sample
JP7326778B2 (ja) * 2018-03-19 2023-08-16 株式会社リコー 核酸試料含有容器、核酸試料含有容器の製造方法、核酸試料含有容器の製造装置及び核酸試料含有容器の製造プログラム、並びに、核酸試料
EP3556466A1 (en) * 2018-04-18 2019-10-23 HiFiBiO SAS Microfluidic method for single cell analysis
CA3061157C (en) * 2018-09-06 2023-11-21 Nicoya Lifesciences, Inc. Plasmon resonance (pr) system and instrument, digital microfluidic (dmf) cartridge, and methods of using localized surface plasmon resonance (lspr) for analysis of analytes
EP3629020B1 (en) 2018-09-25 2021-10-27 bioMérieux Microfluidic droplet-based assay process and apparatus
CN109647550B (zh) * 2018-12-20 2020-04-17 北京交通大学 一种用于精准控制液滴融合的磁性液体液滴实验芯片
CN109865542B (zh) 2019-03-25 2020-05-22 浙江大学 基于弧形斜指换能器的微颗粒多通道分时分离装置及方法
CN111733138B (zh) * 2020-07-30 2021-03-30 首都医科大学附属北京友谊医院 一种循环肿瘤细胞高通量磁力分选方法
JP7322857B2 (ja) * 2020-11-02 2023-08-08 株式会社三洋物産 遊技機
JP7322858B2 (ja) * 2020-11-02 2023-08-08 株式会社三洋物産 遊技機
KR102394807B1 (ko) * 2021-10-20 2022-05-06 비테스코 테크놀로지스 게엠베하 하이사이드 스위치의 고장 진단 장치 및 방법
CN117953476A (zh) * 2024-02-02 2024-04-30 深圳大学 一种液滴数字酶联免疫吸附测定方法、装置及相关介质

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US7214346B2 (en) 2001-02-07 2007-05-08 Massachusetts Institute Of Technology Optoelectronic detection system
US7629124B2 (en) * 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
EP3536396B1 (en) 2006-08-07 2022-03-30 The President and Fellows of Harvard College Fluorocarbon emulsion stabilizing surfactants
ES2611996T3 (es) * 2006-11-20 2017-05-11 Nanotemper Technologies Gmbh Caracterización termo-óptica rápida de partículas
WO2009011808A1 (en) 2007-07-13 2009-01-22 President And Fellows Of Harvard College Droplet-based selection
CN101939649B (zh) * 2008-01-07 2013-10-23 卢米耐克斯公司 从复杂样品基质中对细胞进行分离和计数
WO2010005593A1 (en) * 2008-07-11 2010-01-14 President And Fellows Of Harvard College Systems and methods of droplet-based selection
FR2934050B1 (fr) 2008-07-15 2016-01-29 Univ Paris Curie Procede et dispositif de lecture d'une emulsion
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CN103930210B (zh) * 2011-09-19 2018-06-12 国家科学研究中心 微流体系统
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Also Published As

Publication number Publication date
EP3207373A1 (fr) 2017-08-23
EP3207373B1 (fr) 2019-02-27
FR3027396B1 (fr) 2016-11-25
JP2020183966A (ja) 2020-11-12
US20170307626A1 (en) 2017-10-26
CN107110854A (zh) 2017-08-29
JP6775498B2 (ja) 2020-10-28
WO2016059182A1 (fr) 2016-04-21
CN107110854B (zh) 2019-07-26
FR3027396A1 (fr) 2016-04-22
US10416168B2 (en) 2019-09-17
JP2017532560A (ja) 2017-11-02

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