DK3374525T3 - Højeffektiv konstruktion af dna-biblioteker - Google Patents

Højeffektiv konstruktion af dna-biblioteker Download PDF

Info

Publication number
DK3374525T3
DK3374525T3 DK16865029.9T DK16865029T DK3374525T3 DK 3374525 T3 DK3374525 T3 DK 3374525T3 DK 16865029 T DK16865029 T DK 16865029T DK 3374525 T3 DK3374525 T3 DK 3374525T3
Authority
DK
Denmark
Prior art keywords
dna libraries
efficient construction
efficient
construction
libraries
Prior art date
Application number
DK16865029.9T
Other languages
Danish (da)
English (en)
Inventor
Christopher K Raymond
Lee P Lim
Original Assignee
Resolution Bioscience Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Resolution Bioscience Inc filed Critical Resolution Bioscience Inc
Application granted granted Critical
Publication of DK3374525T3 publication Critical patent/DK3374525T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK16865029.9T 2015-11-11 2016-11-10 Højeffektiv konstruktion af dna-biblioteker DK3374525T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562254110P 2015-11-11 2015-11-11
PCT/US2016/061395 WO2017083562A1 (en) 2015-11-11 2016-11-10 High efficiency construction of dna libraries

Publications (1)

Publication Number Publication Date
DK3374525T3 true DK3374525T3 (da) 2021-04-06

Family

ID=58695623

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16865029.9T DK3374525T3 (da) 2015-11-11 2016-11-10 Højeffektiv konstruktion af dna-biblioteker

Country Status (13)

Country Link
US (2) US11339391B2 (enExample)
EP (2) EP3889257A1 (enExample)
JP (4) JP6913089B2 (enExample)
KR (1) KR102788327B1 (enExample)
CN (2) CN115044645A (enExample)
AU (1) AU2016353133B2 (enExample)
CA (1) CA3004435A1 (enExample)
DK (1) DK3374525T3 (enExample)
ES (1) ES2856598T3 (enExample)
IL (2) IL285202B2 (enExample)
MX (2) MX2018005858A (enExample)
RU (1) RU2018121254A (enExample)
WO (1) WO2017083562A1 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2929056A4 (en) 2012-12-10 2016-11-09 Resolution Bioscience Inc METHODS FOR TARGETED GENOMIC ANALYSIS
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
RU2018121254A (ru) 2015-11-11 2019-12-16 Резолюшн Байосайенс, Инк. Высокоэффективное построение библиотек днк
WO2018039463A1 (en) * 2016-08-25 2018-03-01 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in dna samples
JP2020527340A (ja) * 2017-06-30 2020-09-10 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア セルフリーｄｎａ中のｄｎａメチル化を評価するための方法およびシステム
AU2018328324B2 (en) 2017-09-11 2024-12-12 Ludwig Institute For Cancer Research Ltd Selective labeling of 5-methylcytosine in circulating cell-free DNA
AR113802A1 (es) 2017-10-27 2020-06-10 Juno Diagnostics Inc Dispositivos, sistemas y métodos para la biopsia líquida de un volumen ultra bajo
EP3762513A1 (en) * 2018-03-08 2021-01-13 St. Johns University Circulating serum cell-free dna biomarkers and methods
AU2019244115A1 (en) 2018-03-30 2020-11-19 Juno Diagnostics, Inc. Deep learning-based methods, devices, and systems for prenatal testing
CN119753091A (zh) 2018-04-02 2025-04-04 格里尔公司 用于扩增与癌症相关联的cfDNA分子的化验板
DK3827011T3 (da) 2018-07-24 2024-07-08 Salish Bioscience Inc Fremgangsmåder og sammensætning til målrettet genomisk analyse
WO2020069350A1 (en) 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel
US11781169B2 (en) * 2018-10-05 2023-10-10 Microsoft Technology Licensing, Llc Enzymatic DNA repair
CN111074353B (zh) * 2018-10-18 2023-10-13 深圳华大智造科技股份有限公司 全基因组甲基化文库单链建库方法和得到的全基因组甲基化文库
US11926821B2 (en) * 2018-10-22 2024-03-12 The Chinese University Of Hong Kong Cell-free DNA quality
CN109576799B (zh) * 2018-11-30 2022-04-26 深圳安吉康尔医学检验实验室 Fh测序文库的构建方法和引物组及试剂盒
US20220162591A1 (en) * 2019-03-27 2022-05-26 Juno Diagnostics, Inc. Optimized ultra-low volume liquid biopsy methods, systems, and devices
GB202013141D0 (en) 2020-08-21 2020-10-07 Univ College Cardiff Consultants Ltd A screening method for the detection of DNA damage
PT4211246T (pt) * 2020-09-08 2025-07-30 Resolution Bioscience Inc Adaptadores e métodos para construção de alta eficiência de bibliotecas genéticas e análise genética
CN114317528A (zh) * 2020-09-30 2022-04-12 北京吉因加科技有限公司 特异分子标签umi组、混合特异分子标签接头及应用

Family Cites Families (106)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5591582A (en) 1985-07-23 1997-01-07 The Board Of Rijks Universiteit Leiden Methods for detecting activated RAS oncogenes
US5888731A (en) 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
JP2002511767A (ja) 1997-08-28 2002-04-16 ピーイー コーポレイション（エヌワイ） 化学切断による、核酸における変異の改良された検出
US6480791B1 (en) 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
US6812341B1 (en) 2001-05-11 2004-11-02 Ambion, Inc. High efficiency mRNA isolation methods and compositions
US7183052B2 (en) 2001-07-17 2007-02-27 Stratagene California Methods for detection of a target nucleic acid using multi-subunit probes
WO2003020979A1 (en) 2001-08-31 2003-03-13 Rosetta Inpharmactis Llc. Methods for preparing nucleic acid samples
US7361821B2 (en) 2002-09-20 2008-04-22 Intel Corporation Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading
JP4441623B2 (ja) * 2002-12-11 2010-03-31 学校法人慶應義塾 対応付け分子およびその構成要素のライブラリーの製造方法および利用方法
TW200506052A (en) 2003-05-07 2005-02-16 Takara Bio Inc Method of analyzing DNA region
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
WO2007006091A1 (en) 2005-07-07 2007-01-18 Athlomics Pty Ltd Polynucleotide marker genes and their expression, for diagnosis of endotoxemia
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US7939645B2 (en) 2006-01-06 2011-05-10 Agilent Technologies, Inc Reaction buffer composition for nucleic acid replication with packed DNA polymerases
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US8383338B2 (en) 2006-04-24 2013-02-26 Roche Nimblegen, Inc. Methods and systems for uniform enrichment of genomic regions
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008045158A1 (en) 2006-10-10 2008-04-17 Illumina, Inc. Compositions and methods for representational selection of nucleic acids fro complex mixtures using hybridization
US12180549B2 (en) 2007-07-23 2024-12-31 The Chinese University Of Hong Kong Diagnosing fetal chromosomal aneuploidy using genomic sequencing
WO2009023676A1 (en) 2007-08-12 2009-02-19 Integrated Dna Technologies, Inc. Microarray system with improved sequence specificity
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
JP5362738B2 (ja) * 2007-12-05 2013-12-11 コンプリート・ジェノミックス・インコーポレイテッド シーケンシング反応における効率のよい塩基決定
WO2009091798A1 (en) 2008-01-16 2009-07-23 Helicos Biosciences Corporation Quantitative genetic analysis
US8202691B2 (en) 2008-01-25 2012-06-19 Illumina, Inc. Uniform fragmentation of DNA using binding proteins
CN102203273A (zh) * 2008-09-09 2011-09-28 生命技术公司 生成基因特异性的文库的方法
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US8728764B2 (en) 2008-10-02 2014-05-20 Illumina Cambridge Limited Nucleic acid sample enrichment for sequencing applications
WO2010068856A1 (en) 2008-12-11 2010-06-17 Trustees Of Boston University Isolation of rna
DK2414547T3 (da) 2009-04-02 2014-06-16 Fluidigm Corp Multiprimer-amplifikationsmetode til stregkodning af målnukleinsyrer
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
US20110003301A1 (en) 2009-05-08 2011-01-06 Life Technologies Corporation Methods for detecting genetic variations in dna samples
HUE030765T2 (en) 2009-08-25 2017-05-29 Illumina Inc A method for selecting and amplifying polynucleotides
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US10388403B2 (en) 2010-01-19 2019-08-20 Verinata Health, Inc. Analyzing copy number variation in the detection of cancer
US10378064B1 (en) 2010-04-16 2019-08-13 Chronix Biomedical Analyzing circulating nucleic acids to identify a biomarker representative of cancer presented by a patient population
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
EP2580378A4 (en) 2010-06-08 2014-01-01 Nugen Technologies Inc MULTIPLEX SEQUENCING METHODS AND COMPOSITION
EP2426217A1 (en) 2010-09-03 2012-03-07 Centre National de la Recherche Scientifique (CNRS) Analytical methods for cell free nucleic acids and applications
US9309556B2 (en) 2010-09-24 2016-04-12 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target DNA using immobilized primers
EA038374B1 (ru) 2010-11-30 2021-08-17 Те Чайниз Юниверсити Ов Гонконг Способ анализа биологического образца организма для выявления хромосомных делеций или амплификаций, ассоциированных с раком
JP6054303B2 (ja) 2010-12-30 2016-12-27 ファウンデーション メディシン インコーポレイテッドＦｏｕｎｄａｔｉｏｎ Ｍｅｄｉｃｉｎｅ， Ｉｎｃ． 腫瘍試料の多重遺伝子分析の最適化
CA2824387C (en) 2011-02-09 2019-09-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
EP2697392B1 (en) 2011-04-12 2016-03-02 Verinata Health, Inc Resolving genome fractions using polymorphism counts
EP3907297A1 (en) 2011-04-15 2021-11-10 The Johns Hopkins University Safe sequencing system
WO2013056178A2 (en) 2011-10-14 2013-04-18 Foundation Medicine, Inc. Novel estrogen receptor mutations and uses thereof
CN103103624B (zh) * 2011-11-15 2014-12-31 深圳华大基因科技服务有限公司 高通量测序文库的构建方法及其应用
US10227587B2 (en) 2012-01-10 2019-03-12 Berry Genomics Co., Ltd. Method for constructing a plasma DNA sequencing library
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
HUE051845T2 (hu) 2012-03-20 2021-03-29 Univ Washington Through Its Center For Commercialization Módszerek a tömegesen párhuzamos DNS-szekvenálás hibaarányának csökkentésére duplex konszenzus szekvenálással
US20130288915A1 (en) 2012-04-27 2013-10-31 Htg Molecular Diagnostics, Inc. Compositions and methods for alk molecular testing
AU2013267609C1 (en) 2012-05-31 2019-01-03 Board Of Regents, The University Of Texas System Method for accurate sequencing of DNA
EP2872629B1 (en) 2012-07-03 2019-09-04 Integrated DNA Technologies Inc. Tm-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection
HUE045880T2 (hu) 2012-07-31 2020-01-28 Novartis Ag A humán double minute 2 (MDM2) inhibitorra való érzékenységgel összefüggõ markerek
AU2013296237B2 (en) 2012-08-03 2019-05-16 Foundation Medicine, Inc. Human papilloma virus as predictor of cancer prognosis
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
CN110872617A (zh) 2012-09-04 2020-03-10 夸登特健康公司 检测稀有突变和拷贝数变异的系统和方法
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
CA2886605A1 (en) 2012-09-28 2014-04-03 Cepheid Two-primer pcr for microrna multiplex assay
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA2887094C (en) 2012-10-04 2021-09-07 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20150291953A1 (en) 2012-11-02 2015-10-15 Enzymatics Inc. Methods and kits for nucleic acid sample preparation for sequencing
HK1214830A1 (zh) 2012-11-05 2016-08-05 Foundation Medicine, Inc. 新型ntrk1融合分子及其应用
EP2929056A4 (en) 2012-12-10 2016-11-09 Resolution Bioscience Inc METHODS FOR TARGETED GENOMIC ANALYSIS
EP2931922B1 (en) 2012-12-14 2018-10-17 Chronix Biomedical Personalized biomarkers for cancer
US11158425B2 (en) 2013-01-05 2021-10-26 Foundation Medicine, Inc. System and method for managing genomic information
EP2958574A4 (en) 2013-01-23 2016-11-02 Reproductive Genetics And Technology Solutions Llc COMPOSITIONS AND METHODS FOR GENETIC ANALYSIS OF EMBRYOS
US10745686B2 (en) 2013-02-08 2020-08-18 Qiagen Gmbh Method for separating DNA by size
CA3156663A1 (en) 2013-03-15 2014-09-18 Verinata Health, Inc. Generating cell-free dna libraries directly from blood
ES2831148T3 (es) 2013-03-15 2021-06-07 Univ Leland Stanford Junior Identificación y uso de marcadores tumorales de ácido nucleico circulante
AU2014262481A1 (en) 2013-05-10 2015-11-26 Foundation Medicine, Inc. Analysis of genetic variants
US20150072344A1 (en) 2013-09-10 2015-03-12 Imdaptive Incorporated Barcoded Universal Marker Indicator (BUMI) Tags
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
US9546399B2 (en) 2013-11-13 2017-01-17 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
CN103668471B (zh) * 2013-12-19 2015-09-30 上海交通大学 一种构建dna高通量测序文库的方法及其配套试剂盒
ES2784450T3 (es) 2013-12-28 2020-09-25 Guardant Health Inc Métodos y sistemas para detectar variantes genéticas
HUE058149T2 (hu) * 2014-01-31 2022-07-28 Swift Biosciences Inc Javított eljárások DNS-szubsztrátok feldolgozására
WO2015134552A1 (en) * 2014-03-03 2015-09-11 Swift Biosciences, Inc. Enhanced adaptor ligation
US10975371B2 (en) 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
CA2953469A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
CA2957633A1 (en) 2014-08-06 2016-02-11 Nugen Technologies, Inc. Digital measurements from targeted sequencing
DK3194612T3 (da) * 2014-08-22 2024-10-14 Resolution Bioscience Inc Fremgangsmåder til kvantitativ genetisk analyse af cellefrit dna
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
CN106661636A (zh) * 2014-09-05 2017-05-10 凯杰有限公司 衔接子连接的扩增子的制备
EP3192869B1 (en) * 2014-09-12 2019-03-27 MGI Tech Co., Ltd. Isolated oligonucleotide and use thereof in nucleic acid sequencing
WO2016040901A1 (en) * 2014-09-12 2016-03-17 The Board Of Trustees Of The Leland Stanford Junior University Identification and use of circulating nucleic acids
EP3227464B1 (en) 2014-12-05 2022-04-20 Foundation Medicine, Inc. Multigene analysis of tumor samples
CN107750277B (zh) 2014-12-12 2021-11-09 维里纳塔健康股份有限公司 使用无细胞dna片段大小来确定拷贝数变化
EP4574996B1 (en) 2014-12-31 2025-11-05 Guardant Health, Inc. Detection and treatment of disease exhibiting disease cell heterogeneity and systems and methods for communicating test results
ES2908347T3 (es) 2015-02-10 2022-04-28 Univ Hong Kong Chinese Detección de mutaciones para cribado de cáncer y análisis fetal
EP4279612B1 (en) 2015-07-23 2025-07-02 The Chinese University of Hong Kong Analysis of fragmentation patterns of cell-free dna
US11756655B2 (en) 2015-10-09 2023-09-12 Guardant Health, Inc. Population based treatment recommender using cell free DNA
JP6824973B2 (ja) 2015-10-10 2021-02-03 ガーダント ヘルス， インコーポレイテッド 無細胞ｄｎａ分析における遺伝子融合検出の方法および応用
RU2018121254A (ru) 2015-11-11 2019-12-16 Резолюшн Байосайенс, Инк. Высокоэффективное построение библиотек днк
US10095831B2 (en) 2016-02-03 2018-10-09 Verinata Health, Inc. Using cell-free DNA fragment size to determine copy number variations
EP3423828B1 (en) 2016-02-29 2025-08-27 Foundation Medicine, Inc. Methods and systems for evaluating tumor mutational burden
WO2017214557A1 (en) 2016-06-10 2017-12-14 Counsyl, Inc. Nucleic acid sequencing adapters and uses thereof
WO2018039463A1 (en) 2016-08-25 2018-03-01 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in dna samples
ES2840003T3 (es) 2016-09-30 2021-07-06 Guardant Health Inc Métodos para análisis multi-resolución de ácidos nucleicos libres de células
US9850523B1 (en) 2016-09-30 2017-12-26 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
CA3043476A1 (en) * 2016-11-17 2018-05-24 Seracare Life Sciences, Inc. Methods for preparing dna reference materials and controls
JP7048609B2 (ja) 2016-12-09 2022-04-05 ボリアル ジェノミクス， インコーポレイテッド 連結型ライゲーション
CA3057163A1 (en) 2017-04-14 2018-10-18 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
CN114901818B (zh) 2018-11-21 2025-01-10 安捷伦科技有限公司 靶向核酸文库形成的方法

Also Published As

Publication number Publication date
JP7318054B2 (ja) 2023-07-31
KR102788327B1 (ko) 2025-03-27
KR20180113973A (ko) 2018-10-17
BR112018009606A2 (pt) 2018-11-06
CN108431233B (zh) 2022-04-01
MX2018005858A (es) 2019-02-20
US20180245072A1 (en) 2018-08-30
CA3004435A1 (en) 2017-05-18
JP2023113960A (ja) 2023-08-16
CN115044645A (zh) 2022-09-13
JP2021073252A (ja) 2021-05-13
EP3374525B1 (en) 2021-01-20
BR112018009606A8 (pt) 2019-02-26
RU2018121254A (ru) 2019-12-16
EP3374525A4 (en) 2019-05-29
EP3374525A1 (en) 2018-09-19
CN108431233A (zh) 2018-08-21
JP2022105062A (ja) 2022-07-12
JP2019504618A (ja) 2019-02-21
AU2016353133B2 (en) 2022-12-08
ES2856598T3 (es) 2021-09-27
US20220267763A1 (en) 2022-08-25
EP3889257A1 (en) 2021-10-06
MX2023008338A (es) 2023-07-25
IL285202B2 (en) 2025-03-01
JP6913089B2 (ja) 2021-08-04
IL259237B (en) 2021-08-31
IL285202A (en) 2021-08-31
IL259237A (en) 2018-07-31
AU2016353133A1 (en) 2018-05-24
JP7223788B2 (ja) 2023-02-16
US11339391B2 (en) 2022-05-24
WO2017083562A1 (en) 2017-05-18
IL285202B1 (en) 2024-11-01
NZ742379A (en) 2024-12-20

Similar Documents

Publication Publication Date Title
DK3374525T3 (da) Højeffektiv konstruktion af dna-biblioteker
IL264966B (en) De novo synthesized nucleic acid libraries
EP3359661A4 (en) MULTIPLEXED GEMONEDITATION
DK3377059T3 (da) Hæmmere af cxcr2
HUE056911T2 (hu) Genetikai konstrukció
DK3137169T3 (da) Hæmmere af lysin-specifik demethylase-1
DK3180331T3 (da) Polymorfer af selinexor
MA42692A (fr) Compositions biopharmaceutiques
EP3406336A4 (en) NANO FOIL OF CORE CASE STRUCTURE TYPE
DK3685847T3 (da) Modulatorer af komplementaktivitet
EP3619321A4 (en) DP04 TYPE POLYMERASE VARIANTS
DK3307436T3 (da) Mikrofluidanordning
DK3544416T3 (da) Applikation af væsker
PL4030014T3 (pl) Wkładka dystansowa ścienna
EP3342870A4 (en) CORYNEBACTERIUM
DK3368044T3 (da) Behandling af hepatisk steatose-relateret oligo-ovulation
IL254502B (en) Solid forms of menaquinols
EP3338088A4 (en) BIOMARKER FOR CORONARY DISEASE
DE112016005541A5 (de) Arylierungsverfahren
EP3378566A4 (en) CENTRIFUGE
DK3310331T3 (da) Injicerbare farmaceutiske formuleringer af lefamulin
DK3430004T3 (da) Faststofformer af nilotinibsalte
DK3173071T3 (da) Formularing af maropitant
DK3289363T3 (da) Specifik påvisning af clusterin isoformer
EP3342869A4 (en) CORYNEBACTERIUM