DK3169832T3 - Method for identifying high-affinity complexes of two ligands and a receptor - Google Patents
Method for identifying high-affinity complexes of two ligands and a receptor Download PDFInfo
- Publication number
- DK3169832T3 DK3169832T3 DK15744501.6T DK15744501T DK3169832T3 DK 3169832 T3 DK3169832 T3 DK 3169832T3 DK 15744501 T DK15744501 T DK 15744501T DK 3169832 T3 DK3169832 T3 DK 3169832T3
- Authority
- DK
- Denmark
- Prior art keywords
- ligands
- rna
- bases
- stranded dna
- receptor
- Prior art date
Links
- 239000003446 ligand Substances 0.000 title claims description 237
- 238000000034 method Methods 0.000 title claims description 64
- 108020004414 DNA Proteins 0.000 claims description 147
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 102
- 230000000295 complement effect Effects 0.000 claims description 50
- 102000053602 DNA Human genes 0.000 claims description 44
- 108020004682 Single-Stranded DNA Proteins 0.000 claims description 25
- 238000009396 hybridization Methods 0.000 claims description 20
- 238000010494 dissociation reaction Methods 0.000 claims description 13
- 230000005593 dissociations Effects 0.000 claims description 13
- 108090000623 proteins and genes Proteins 0.000 claims description 12
- 102000004169 proteins and genes Human genes 0.000 claims description 12
- 239000000126 substance Substances 0.000 claims description 12
- 102000040650 (ribonucleotides)n+m Human genes 0.000 claims description 11
- 102000003960 Ligases Human genes 0.000 claims description 7
- 108090000364 Ligases Proteins 0.000 claims description 7
- 238000001712 DNA sequencing Methods 0.000 claims description 6
- 108091023037 Aptamer Proteins 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 claims description 3
- 150000001720 carbohydrates Chemical class 0.000 claims description 3
- 230000003993 interaction Effects 0.000 claims description 3
- 150000002632 lipids Chemical class 0.000 claims description 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 3
- 238000004566 IR spectroscopy Methods 0.000 claims description 2
- 229920002684 Sepharose Polymers 0.000 claims description 2
- 229920001222 biopolymer Polymers 0.000 claims description 2
- 239000000919 ceramic Substances 0.000 claims description 2
- 238000004817 gas chromatography Methods 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 2
- 239000000017 hydrogel Substances 0.000 claims description 2
- 230000001771 impaired effect Effects 0.000 claims description 2
- 238000004949 mass spectrometry Methods 0.000 claims description 2
- 239000000758 substrate Substances 0.000 claims description 2
- 229920001059 synthetic polymer Polymers 0.000 claims description 2
- 238000002474 experimental method Methods 0.000 description 19
- 239000012530 fluid Substances 0.000 description 11
- 238000001816 cooling Methods 0.000 description 10
- 230000035945 sensitivity Effects 0.000 description 9
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 8
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 8
- 238000010438 heat treatment Methods 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 6
- 238000011534 incubation Methods 0.000 description 6
- DEQPBRIACBATHE-FXQIFTODSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-2-iminopentanoic acid Chemical compound N1C(=O)N[C@@H]2[C@H](CCCC(=N)C(=O)O)SC[C@@H]21 DEQPBRIACBATHE-FXQIFTODSA-N 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 229930024421 Adenine Natural products 0.000 description 4
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 4
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 4
- 229960000643 adenine Drugs 0.000 description 4
- 229940104302 cytosine Drugs 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 4
- 108010090804 Streptavidin Proteins 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000002860 competitive effect Effects 0.000 description 3
- 229930010555 Inosine Natural products 0.000 description 2
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 239000011230 binding agent Substances 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 229960003786 inosine Drugs 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 229940113082 thymine Drugs 0.000 description 2
- 229940035893 uracil Drugs 0.000 description 2
- 108091008102 DNA aptamers Proteins 0.000 description 1
- 108091008103 RNA aptamers Proteins 0.000 description 1
- 238000003559 RNA-seq method Methods 0.000 description 1
- 239000012062 aqueous buffer Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 230000009918 complex formation Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000036963 noncompetitive effect Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000036967 uncompetitive effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/088—Channel loops
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1861—Means for temperature control using radiation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1894—Cooling means; Cryo cooling
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Dispersion Chemistry (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102014213783.7A DE102014213783B3 (de) | 2014-07-16 | 2014-07-16 | Verfahren zur Identifizierung hochaffiner Komplexe aus zwei Liganden und einem Rezeptor, Vorrichtung zur Durchführung des Verfahrens und selbst-assemblierende chemische Bibliothek zur Verwendung in dem Verfahren |
| PCT/EP2015/065874 WO2016008822A2 (de) | 2014-07-16 | 2015-07-10 | Verfahren zur identifizierung hochaffiner komplexe aus zwei liganden und einem rezeptor, vorrichtung zur durchführung des verfahrens und selbst-assemblierende chemische bibliothek zur verwendung in dem verfahren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3169832T3 true DK3169832T3 (en) | 2019-03-25 |
Family
ID=53192981
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15744501.6T DK3169832T3 (en) | 2014-07-16 | 2015-07-10 | Method for identifying high-affinity complexes of two ligands and a receptor |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US11365439B2 (https=) |
| EP (2) | EP3169832B1 (https=) |
| JP (2) | JP6831776B2 (https=) |
| CA (1) | CA2955087C (https=) |
| DE (1) | DE102014213783B3 (https=) |
| DK (1) | DK3169832T3 (https=) |
| ES (2) | ES2712899T3 (https=) |
| WO (1) | WO2016008822A2 (https=) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017218293A1 (en) | 2016-06-16 | 2017-12-21 | Richard Edward Watts | Oligonucleotide directed and recorded combinatorial synthesis of encoded probe molecules |
| US11795580B2 (en) | 2017-05-02 | 2023-10-24 | Haystack Sciences Corporation | Molecules for verifying oligonucleotide directed combinatorial synthesis and methods of making and using the same |
| CN107652453B (zh) * | 2017-09-26 | 2020-05-05 | 天津工业大学 | 接枝rgd短肽温敏可注射水凝胶及其制备方法和应用 |
| EP3604525B1 (en) * | 2018-08-02 | 2021-03-10 | TU Dresden | Method for providing a dna-encoded library, dna-encoded library and method of decoding a dna-encoded library |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5512439A (en) * | 1988-11-21 | 1996-04-30 | Dynal As | Oligonucleotide-linked magnetic particles and uses thereof |
| US7018793B1 (en) * | 1995-12-07 | 2006-03-28 | Diversa Corporation | Combinatorial screening of mixed populations of organisms |
| DE19619373A1 (de) * | 1996-05-14 | 1997-11-20 | Hoechst Ag | Neue Substanzbibliothek und damit hergestellte supramolekulare Komplexe |
| EP1384022A4 (en) * | 2001-04-06 | 2004-08-04 | California Inst Of Techn | NUCLEIC ACID AMPLIFICATION USING MICROFLUID DEVICES |
| AU2002310846B2 (en) * | 2002-03-08 | 2008-03-20 | Eidgenossische Technische Hochschule Zurich | Encoded self-assembling chemical libraries (ESACHEL) |
| CN101410530B (zh) * | 2003-04-18 | 2013-03-27 | 贝克顿·迪金森公司 | 免疫-扩增 |
| US7229769B2 (en) * | 2005-03-25 | 2007-06-12 | Illumina, Inc. | Compositions and methods for detecting protease activity |
| EP2064348B1 (en) * | 2006-09-18 | 2012-02-08 | Ensemble Therapeutics Corporation | Receptor family profiling |
| JP2009034052A (ja) * | 2007-08-02 | 2009-02-19 | Canon Inc | ハイブリダイゼーション方法および装置 |
| EP2138587A1 (en) * | 2008-06-23 | 2009-12-30 | Koninklijke Philips Electronics N.V. | Amplification of nucleic acids using temperature zones |
| EP2291539B1 (en) * | 2008-06-25 | 2018-03-14 | Okura, Michael | Apparatus for melting curve analysis of nucleic acids in microarray format |
| EP2446033B1 (en) * | 2009-06-22 | 2013-10-23 | Université de Strasbourg | Method of preparing an adduct |
| US10190986B2 (en) * | 2011-06-06 | 2019-01-29 | Abbott Laboratories | Spatially resolved ligand-receptor binding assays |
| BE1020816A5 (fr) * | 2012-07-04 | 2014-05-06 | Coris Bioconcept Sprl | Procede et dispositif pour la detection rapide de sequences nucleotidiques amplifiees. |
| US9708658B2 (en) * | 2013-03-19 | 2017-07-18 | New England Biolabs, Inc. | Enrichment of target sequences |
-
2014
- 2014-07-16 DE DE102014213783.7A patent/DE102014213783B3/de active Active
-
2015
- 2015-07-10 US US15/326,172 patent/US11365439B2/en active Active
- 2015-07-10 EP EP15744501.6A patent/EP3169832B1/de active Active
- 2015-07-10 CA CA2955087A patent/CA2955087C/en active Active
- 2015-07-10 EP EP18207435.1A patent/EP3477299B1/de active Active
- 2015-07-10 ES ES15744501T patent/ES2712899T3/es active Active
- 2015-07-10 JP JP2017502621A patent/JP6831776B2/ja active Active
- 2015-07-10 DK DK15744501.6T patent/DK3169832T3/en active
- 2015-07-10 WO PCT/EP2015/065874 patent/WO2016008822A2/de not_active Ceased
- 2015-07-10 ES ES18207435T patent/ES3002709T3/es active Active
-
2021
- 2021-01-29 JP JP2021013303A patent/JP7112540B2/ja active Active
-
2022
- 2022-06-07 US US17/834,197 patent/US20220298552A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20170198336A1 (en) | 2017-07-13 |
| JP6831776B2 (ja) | 2021-02-17 |
| EP3169832B1 (de) | 2018-12-05 |
| WO2016008822A2 (de) | 2016-01-21 |
| EP3477299C0 (de) | 2024-12-11 |
| EP3477299B1 (de) | 2024-12-11 |
| EP3477299A1 (de) | 2019-05-01 |
| JP2021092576A (ja) | 2021-06-17 |
| WO2016008822A3 (de) | 2016-05-19 |
| EP3169832A2 (de) | 2017-05-24 |
| JP2017529062A (ja) | 2017-10-05 |
| CA2955087A1 (en) | 2016-01-21 |
| CA2955087C (en) | 2023-03-07 |
| ES2712899T3 (es) | 2019-05-16 |
| ES3002709T3 (en) | 2025-03-07 |
| US20220298552A1 (en) | 2022-09-22 |
| JP7112540B2 (ja) | 2022-08-03 |
| DE102014213783B3 (de) | 2015-06-18 |
| US11365439B2 (en) | 2022-06-21 |
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