DK3013379T3 - Lipofyldning med ex-vivo-ekspanderede, fra fedtvæv stammende stamceller, til kosmetisk brystfyldning eller til ansigtsfyldning og/eller -foryngelse - Google Patents
Lipofyldning med ex-vivo-ekspanderede, fra fedtvæv stammende stamceller, til kosmetisk brystfyldning eller til ansigtsfyldning og/eller -foryngelse Download PDFInfo
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- DK3013379T3 DK3013379T3 DK14735518.4T DK14735518T DK3013379T3 DK 3013379 T3 DK3013379 T3 DK 3013379T3 DK 14735518 T DK14735518 T DK 14735518T DK 3013379 T3 DK3013379 T3 DK 3013379T3
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- Prior art keywords
- ascs
- adipose tissue
- filling
- stem cells
- fat
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- 238000003780 insertion Methods 0.000 claims 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/91—Injection
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/06—Flowable or injectable implant compositions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/04—Materials or treatment for tissue regeneration for mammary reconstruction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/34—Materials or treatment for tissue regeneration for soft tissue reconstruction
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Dermatology (AREA)
- Epidemiology (AREA)
- Botany (AREA)
- Transplantation (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Birds (AREA)
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- Organic Chemistry (AREA)
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- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Rheumatology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
Claims (7)
1. Fremgangsmåde til fremstilling af en sammensætning omfattende ex-vivo ekspanderede primærkultur (PO) stamceller stammende fra fedtvæv (ASC'er) blandet med høstet fedtvæv; hvilken fremgangsmåde omfatter: - ex-vivo ekspandering af ASC'er fra en isoleret stromal vaskulær fraktion (SVF) i et vækstmedie bestående af Dulbecco's modificeret Eagle's medie (DMEM) eller alpha minimal essentiel medie (a-MEM),1-5% penicillin-streptomycin, 1-5 lU/ml konserveringsmiddelfri heparin og 2-20% pooled humant thrombocytlysat (pHPL), - høstning af de nævnte ASC'er i en primær passage (PO), og - blanding af de nævnte ASC'er med høstet fedtvæv i et blandingsforhold på 20 x 106 - 20 x 107 ASC'er/ml fedt.
2. Sammensætning omfattende ex-vivo ekspanderet primær kultur (PO) fra fedtvæv stammende stamceller (ASC'er) blandet med høstet fedtvæv i et blandingsforhold på i det mindste 20 x 106 - 20 x 107 ASC'er/ml fedt, hvor de nævnte ex-vivo ekspanderede primær kultur (P0) fra fedtvæv stammende stamceller (ASC'er) er dyrket i et vækstmedie bestående af Dulbecco's modificeret Eagle's medie (DMEM) eller alpha minimal essentiel medie (a-MEM),1-5% penicillin-streptomycin, 1-5 lU/ml konserveringsmiddelfri heparin og 2-20% pooled humant thrombocytlysat (pHPL) og som er høstet i den primære passage (P0).
3. Sammensætning ifølge krav 2 til anvendelse som et brystfyldningsmiddel, et ansigtsfyldningsmiddel, til kosmetisk brystfyldning, eller til kosmetisk ansigtsfyldning.
4. Kosmetisk fremgangsmåde til brystfyldning, hvor ex-vivo ekspanderede primær kultur (P0) fra fedtvæv stammende stamceller (ASC'er) blandes med høstet fedtvæv i et blandingsforhold på i det mindste 20 x 106 - 20 x 107 ASC'er/ml fedt, hvor de primære kultur (P0) fra fedtvæv stammende stamceller (ASC'er) er dyrket i et vækstmedie bestående af Dulbecco's modificeret Eagle's medie (DMEM) eller alpha minimal essentiel medie (a-MEM),1-5% penicillin-streptomycin, 1-5 lU/ml konserveringsmiddelfri heparin og 2-20% pooled humant thrombocytlysat (pFIPL) og er høstet i den primære passage (P0), og hvor fedtet injiceres som alikvoter eller som strenge med en lang nål, horisontalt (parallelt med kroppen) ved indsætning af nålen fra adskillige punkter omkring brystvortekanten og ved adskillige punkter ved den under brystet liggende fold, i forskellige retninger og planer for opnåelse af en jævn og naturligt udseende fordeling af transplantatet.
5. Kosmetisk fremgangsmåde til ansigtsfyldning, hvor ex-vivo ekspanderede primær kultur (PO) fra fedtvæv stammende stamceller (ASC'er) blandes med høstet fedtvæv i et blandingsforhold på i det mindste 20 x 106 - 20 x 107 ASC'er/ml fedt, hvor de primære kultur (PO) fra fedtvæv stammende stamceller (ASC'er) er dyrket i et vækstmedie bestående af Dulbecco's modificeret Eagle's medie (DMEM) eller alpha minimal essentiel medie (a-MEM),1-5% penicillin-streptomycin, 1-5 lU/ml konserveringsmiddelfri heparin og 2-20% pooled humant thrombocytlysat (pHPL) og er høstet i den primære passage (PO), og hvor fedttransplantat injiceres som alikvoter eller som strenge med en lang nål, horisontalt (parallelt med overfladen) for at undgå beskadigelse af strukturer udenfor målområdet, og nålen indsættes fra adskillige punkter og i forskellige retninger og planer for opnåelse af en jævn og naturligt udseende fordeling af transplantatet.
6. Kosmetisk fremgangsmåde til ansigtsfyldning, hvor ex-vivo ekspanderede primær kultur (PO) fra fedtvæv stammende stamceller (ASC'er) blandes med høstet fedtvæv i et blandingsforhold på i det mindste 20 x 106 - 20 x 107 ASC'er/ml fedt, hvor de primære kultur (P0) fra fedtvæv stammende stamceller (ASC'er) er dyrket i et vækstmedie bestående af Dulbecco's modificeret Eagle's medie (DMEM) eller alpha minimal essentiel medie (a-MEM),1-5% penicillin-streptomycin, 1-5 lU/ml konserveringsmiddelfri heparin og 2-20% pooled humant thrombocytlysat (pHPL) og er høstet i den primære passage (P0), og hvor ASC'er injiceres i dermis med en tynd skarp nål og jævnt fordelt i målområdet og indstiks- og injektionsstederne syes og post-operativ kompressionsbeklædning påføres donorpositionerne og i visse tilfælde også modtage positionerne.
7. Kosmetisk fremgangsmåde til indføring af et middel i huden, hvor midlet omfatter ex-vivo ekspanderede primær kultur (P0) fra fedtvæv stammende stamceller (ASC'er) blandet med høstet fedtvæv i et blandingsforhold på i det mindste 20 x 106 - 20 x 107 ASC'er/ml fedt, hvor de primære kultur (P0) fra fedtvæv stammende stamceller (ASC'er) er dyrket i et vækstmedie bestående af Dulbecco's modificeret Eagle's medie (DMEM) eller alpha minimal essentiel medie (a-MEM),1-5% penicillin-streptomycin, 1-5 lU/ml konserveringsmiddelfri heparin og 2-20% pooled humant thrombocytlysat (pHPL) og er høstet i den primære passage (P0).
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361839578P | 2013-06-26 | 2013-06-26 | |
| PCT/EP2014/063581 WO2014207135A1 (en) | 2013-06-26 | 2014-06-26 | Lipofilling with ex-vivo expanded adipose tissue-derived stem cells for cosmetic breast filling or for facial filling and/or rejuvenation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3013379T3 true DK3013379T3 (da) | 2017-05-22 |
Family
ID=51063418
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK14735518.4T DK3013379T3 (da) | 2013-06-26 | 2014-06-26 | Lipofyldning med ex-vivo-ekspanderede, fra fedtvæv stammende stamceller, til kosmetisk brystfyldning eller til ansigtsfyldning og/eller -foryngelse |
Country Status (16)
| Country | Link |
|---|---|
| US (2) | US10751276B2 (da) |
| EP (1) | EP3013379B1 (da) |
| JP (1) | JP6440702B2 (da) |
| KR (2) | KR102379417B1 (da) |
| CN (1) | CN105358188B (da) |
| BR (1) | BR112015032570A2 (da) |
| CA (1) | CA2916389C (da) |
| DK (1) | DK3013379T3 (da) |
| ES (1) | ES2620957T3 (da) |
| LT (1) | LT3013379T (da) |
| MX (1) | MX362178B (da) |
| PL (1) | PL3013379T3 (da) |
| PT (1) | PT3013379T (da) |
| RU (1) | RU2660561C2 (da) |
| SI (1) | SI3013379T1 (da) |
| WO (1) | WO2014207135A1 (da) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2016001247A (es) | 2013-07-30 | 2016-08-17 | Musculoskeletal Transplant Foundation | Matrices derivadas de tejido suave acelular y metodos para preparar las mismas. |
| US9931436B2 (en) | 2015-02-02 | 2018-04-03 | Kerastem Technologies LLC | Methods and devices to stimulate the follicular niche using adipose derived regenerative cells and adipose tissue |
| US10912864B2 (en) | 2015-07-24 | 2021-02-09 | Musculoskeletal Transplant Foundation | Acellular soft tissue-derived matrices and methods for preparing same |
| US11052175B2 (en) | 2015-08-19 | 2021-07-06 | Musculoskeletal Transplant Foundation | Cartilage-derived implants and methods of making and using same |
| WO2017195225A1 (en) * | 2016-05-10 | 2017-11-16 | Promoitalia Group Spa | Method for extracting and separating stem cells derived from adipose tissue for aesthetic treatments |
| EP3787702B1 (en) | 2018-05-03 | 2024-08-07 | CollPlant Ltd. | Dermal fillers and applications thereof |
| CN109106983B (zh) * | 2018-10-31 | 2021-04-16 | 郑岩 | 一种脂肪移植材料及其制备方法和应用 |
| RU2716451C1 (ru) * | 2019-03-11 | 2020-03-11 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Волгоградский государственный медицинский университет" Министерства здравоохранения Российской Федерации ФГБОУ ВО ВолгГМУ МЗ РФ | Способ биологической стимуляции адгезиогенеза в плевральной полости при остаточных полостях после перенесённых нагноительных заболеваний лёгких и плевры |
| RU2755347C1 (ru) * | 2020-12-11 | 2021-09-15 | Денис Григорьевич Кайгородов | Композиция для коррекции возрастных изменений кожи и способ ее получения |
| CN112646776A (zh) * | 2021-01-06 | 2021-04-13 | 上海南滨江细胞生物科技有限公司 | 一种用于疤痕修复的自体脂肪源干细胞的提取方法 |
| CN112773937A (zh) * | 2021-01-26 | 2021-05-11 | 北京臻悦医疗美容诊所有限公司 | 一种提高注射脂肪的成活量的方法 |
| CN115040691A (zh) * | 2022-05-26 | 2022-09-13 | 格莱康美生物医学技术(北京)有限公司 | 一种用于丰胸的脂肪填充剂及其制备方法 |
| EP4704933A1 (en) | 2023-05-03 | 2026-03-11 | Volumina Medical SA | Devices and methods for preparing and conditioning biological assemblies for minimally invasive delivery for tissue engineering, cell therapies and other therapeutic applications |
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| US7651684B2 (en) * | 2001-12-07 | 2010-01-26 | Cytori Therapeutics, Inc. | Methods of using adipose tissue-derived cells in augmenting autologous fat transfer |
| RU2275898C1 (ru) * | 2004-10-29 | 2006-05-10 | Закрытое акционерное общество "МИРРА-М" | Способ косметической реабилитации тканей кожи в послеоперационном периоде и других посттравматических состояниях (варианты) |
| EP2617428A1 (en) * | 2006-08-15 | 2013-07-24 | Agency for Science, Technology and Research | Mesenchymal stem cell conditioned medium |
| KR100827660B1 (ko) * | 2006-10-25 | 2008-05-07 | 대한민국 (식품의약품안전청장) | Ｃｄ9를 발현하는 인간 중간엽 줄기세포의 선별 및배양방법 |
| US20080159974A1 (en) * | 2006-11-10 | 2008-07-03 | Steve Harripersad | Lip plumping cosmetic composition |
| KR101738324B1 (ko) | 2009-05-01 | 2017-05-19 | 비미니 테크놀로지스 엘엘씨 | 조직 및 세포 부유화 이식편의 최적화 시스템, 방법 및 조성물 |
| CN102284084A (zh) * | 2011-05-30 | 2011-12-21 | 王影 | 可注射的乳房修复材料、其制备方法及其应用 |
| CN102258810A (zh) * | 2011-07-20 | 2011-11-30 | 王泰华 | 一种富集自体干细胞的脂肪组织隆胸材料的制备方法 |
| DE102011121982B3 (de) * | 2011-12-22 | 2013-04-18 | Dietmar Hopp Stiftung GmbH | Mesenchymale Stammzellen aus Fettgewebe (ADSC) zur Rekonstruktion von Haut und Weichteilgewebe |
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- 2014-06-26 KR KR1020217019144A patent/KR102379417B1/ko not_active Expired - Fee Related
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- 2014-06-26 PT PT147355184T patent/PT3013379T/pt unknown
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| Publication number | Publication date |
|---|---|
| JP6440702B2 (ja) | 2018-12-19 |
| EP3013379B1 (en) | 2017-02-22 |
| CN105358188A (zh) | 2016-02-24 |
| US20160113863A1 (en) | 2016-04-28 |
| EP3013379A1 (en) | 2016-05-04 |
| PT3013379T (pt) | 2017-04-24 |
| RU2015156903A3 (da) | 2018-03-06 |
| CA2916389C (en) | 2019-09-10 |
| KR20210079414A (ko) | 2021-06-29 |
| US20210000736A1 (en) | 2021-01-07 |
| SI3013379T1 (sl) | 2017-06-30 |
| RU2660561C2 (ru) | 2018-07-06 |
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| US10751276B2 (en) | 2020-08-25 |
| RU2015156903A (ru) | 2017-07-27 |
| WO2014207135A1 (en) | 2014-12-31 |
| CN105358188B (zh) | 2019-06-28 |
| MX2015017298A (es) | 2016-07-14 |
| LT3013379T (lt) | 2017-06-12 |
| JP2016523645A (ja) | 2016-08-12 |
| PL3013379T3 (pl) | 2017-08-31 |
| BR112015032570A2 (pt) | 2017-07-25 |
| KR102379417B1 (ko) | 2022-03-25 |
| ES2620957T3 (es) | 2017-06-30 |
| KR102269612B1 (ko) | 2021-06-28 |
| CA2916389A1 (en) | 2014-12-31 |
| KR20160024881A (ko) | 2016-03-07 |
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