DK2970878T3 - PROCEDURES AND REAGENTS FOR MAINTAINING THE LIVELY OF CANCER CELLS IN OPERATIVE REMOVED Tissue - Google Patents
PROCEDURES AND REAGENTS FOR MAINTAINING THE LIVELY OF CANCER CELLS IN OPERATIVE REMOVED Tissue Download PDFInfo
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- DK2970878T3 DK2970878T3 DK14763797.9T DK14763797T DK2970878T3 DK 2970878 T3 DK2970878 T3 DK 2970878T3 DK 14763797 T DK14763797 T DK 14763797T DK 2970878 T3 DK2970878 T3 DK 2970878T3
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- reagent
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- chelator
- chaotrope
- cells
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- A—HUMAN NECESSITIES
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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| US201361798627P | 2013-03-15 | 2013-03-15 | |
| PCT/US2014/029198 WO2014144682A1 (en) | 2013-03-15 | 2014-03-14 | Methods and reagents for maintaining the viability of cancer cells in surgically removed tissue |
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| DK2970878T3 true DK2970878T3 (en) | 2018-09-03 |
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| EP (2) | EP3581643A1 (https=) |
| JP (1) | JP6465857B2 (https=) |
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| SI (1) | SI2970878T1 (https=) |
| SM (1) | SMT201800457T1 (https=) |
| WO (1) | WO2014144682A1 (https=) |
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| ES2684582T3 (es) | 2013-03-15 | 2018-10-03 | Truckee Applied Genomics, Llc | Métodos y reactivos para mantener la viabilidad de células cancerosas en tejido eliminado quirúrgicamente |
| CN107002063A (zh) | 2014-11-25 | 2017-08-01 | 生物辐射实验室股份有限公司 | 精氨酸提高聚合酶储存稳定性 |
| CN107333750A (zh) * | 2017-06-11 | 2017-11-10 | 成都吱吖科技有限公司 | 一种长时血液细胞稳定剂 |
| EP4089180B1 (en) | 2019-09-17 | 2025-06-25 | Longhorn Vaccines and Diagnostics, LLC | Multipurpose compositions for collecting and transporting biological material |
| WO2022010916A1 (en) * | 2020-07-07 | 2022-01-13 | Truckee Applied Genomics, Llc | Cell preservation reagents and methods of use |
| CN117063915B (zh) * | 2023-08-17 | 2026-02-24 | 镜像绮点(上海)细胞技术有限公司 | 原代肿瘤细胞冻存液 |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5409826A (en) * | 1993-06-08 | 1995-04-25 | Coulter Corporation | Preserved, non-infectious control cells prepared by the modulation or modification of normal cells |
| JP2000509018A (ja) * | 1996-03-26 | 2000-07-18 | イーライ・リリー・アンド・カンパニー | 肥満タンパク質製剤 |
| US20010007673A1 (en) | 1999-11-12 | 2001-07-12 | Merrill Seymour Goldenberg | Sustained-release delayed gels |
| US7569342B2 (en) | 1997-12-10 | 2009-08-04 | Sierra Molecular Corp. | Removal of molecular assay interferences |
| US20080064108A1 (en) | 1997-12-10 | 2008-03-13 | Tony Baker | Urine Preservation System |
| WO1999029904A2 (en) | 1997-12-10 | 1999-06-17 | Sierra Diagnostics, Inc. | Methods and reagents for preservation of dna in bodily fluids |
| WO2002046368A1 (fr) * | 2000-12-04 | 2002-06-13 | Humantec Ltd. | Liquide de conservation de cellules et procede de conservation de cellules dans lequel ledit liquide est utilise |
| KR20020066778A (ko) | 2001-02-13 | 2002-08-21 | 한국과학기술연구원 | 체내 난흡수 물질의 흡수촉진용 조성물과 제형 및 그의제조방법 |
| WO2008111981A1 (en) | 2007-03-14 | 2008-09-18 | Sierra Molecular Corporation | Compositions, systems, and methods for preservation of macromolecules |
| AU2003300899A1 (en) * | 2002-12-12 | 2004-06-30 | Oncotech, Inc. | Genes related to sensitivity and resistance to chemotherapeutic drug treatment |
| US8793895B2 (en) * | 2006-02-10 | 2014-08-05 | Praxair Technology, Inc. | Lyophilization system and method |
| CN101617045A (zh) | 2006-09-12 | 2009-12-30 | 谢拉分子公司 | 使用离液剂的缓冲溶液去除核酸的分子分析干扰物 |
| US8080645B2 (en) * | 2007-10-01 | 2011-12-20 | Longhorn Vaccines & Diagnostics Llc | Biological specimen collection/transport compositions and methods |
| US8652782B2 (en) * | 2006-09-12 | 2014-02-18 | Longhorn Vaccines & Diagnostics, Llc | Compositions and methods for detecting, identifying and quantitating mycobacterial-specific nucleic acids |
| JP2010508090A (ja) | 2006-10-27 | 2010-03-18 | シエラ モレキュラー コーポレイション | 貫通可能な隔壁キャップ |
| ES2394815T3 (es) | 2006-12-13 | 2013-02-05 | F. Hoffmann-La Roche Ag | Utilización de TDE para el aislamiento de ácidos nucleicos |
| AU2008224883A1 (en) | 2007-03-14 | 2008-09-18 | Sierra Molecular Corporation | Compositions, systems, and methods for preservation and/or stabilization of a cell and/or macromolecule |
| KR20100102607A (ko) * | 2007-11-12 | 2010-09-24 | 바이파 사이언스 인코포레이티드 | Ｐａｒｐ 억제제를 단독으로 사용하거나 항종양제와 병용하여 자궁암 및 난소암을 치료하는 방법 |
| US20100003748A1 (en) * | 2008-07-03 | 2010-01-07 | Tony Baker | Compositions, systems, and methods for stabilization of a cell and/or macromolecule |
| WO2010028156A2 (en) | 2008-09-04 | 2010-03-11 | Board Of Regents, The University Of Texas System | Dual modality detection of apoptosis |
| US9133343B2 (en) | 2009-11-30 | 2015-09-15 | Enzo Biochem, Inc. | Dyes and compositions, and processes for using same in analysis of protein aggregation and other applications |
| CN102318597B (zh) * | 2011-08-19 | 2013-04-03 | 张雪云 | 一种液基细胞保存液组合物及其制备方法 |
| ES2684582T3 (es) | 2013-03-15 | 2018-10-03 | Truckee Applied Genomics, Llc | Métodos y reactivos para mantener la viabilidad de células cancerosas en tejido eliminado quirúrgicamente |
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