DK2861741T3 - Cho-ekspressionssystem - Google Patents
Cho-ekspressionssystem Download PDFInfo
- Publication number
- DK2861741T3 DK2861741T3 DK13732112.1T DK13732112T DK2861741T3 DK 2861741 T3 DK2861741 T3 DK 2861741T3 DK 13732112 T DK13732112 T DK 13732112T DK 2861741 T3 DK2861741 T3 DK 2861741T3
- Authority
- DK
- Denmark
- Prior art keywords
- gly
- cell line
- ala
- glu
- sequence
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
- C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
- C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2820/00—Vectors comprising a special origin of replication system
- C12N2820/55—Vectors comprising a special origin of replication system from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/60—Vector systems having a special element relevant for transcription from viruses
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Claims (14)
1. Kinesisk hamster-ovarie (CHO)-cellelinje, der omfatter en deoxyribonukleinsyre (DNA)-ekspressionsvektor, og hvor vektoren omfatter en nukleotidsekvens, der koder for en heterolog mammalia-glutaminsyntetase (GS) under kontrol af en promotor fra vakuolerende simianvirus 40 (SV40), herunder SV40-enhanceren, og mindst én ekspressionskassette til ekspression af et rekombinant protein, hvor GS omfatter en sekvens, der er mindst 94,5 % identisk med sekvensen ifølge SEQ ID NO: 1 eller med sekvensen ifølge SEQ ID NO: 2.
2. CHO-cellelinje ifølge krav 1, hvor GS er en human GS.
3. CHO-cellelinje ifølge krav 2, hvor GS omfatter en sekvens ifølge SEQ ID NO: 1.
4. CHO-cellelinje ifølge krav 1, hvor GS er en hunde-GS.
5. CHO-cellelinje ifølge krav 4, hvor hunde-GS omfatter en sekvens ifølge SEQ ID NO: 2.
6. CHO-cellelinje ifølge et hvilket som helst af kravene 1 til 5, hvor det rekombinante protein er et monoklonalt antistof.
7. CHO-cellelinje ifølge krav 6, hvor vektoren omfatter en første ekspressionskassette, der er egnet til kloning af en let kæde af et antistof, og en anden ekspressionskassette, der er egnet til kloning af en tung kæde af et antistof.
8. CHO-cellelinje ifølge et hvilket som helst af kravene 1 til 7, hvor CHO-cellelinjen er cellelinjen, der er deponeret under nr. CCL-61 ved ATCC.
9. CHO-cellelinje ifølge et hvilket som helst af kravene 1 til 8, der er kendetegnet ved, at den åbner mulighed for opnåelse af kloner, der danner mindst 1 mg/1 rekombinant protein ved transfektion af vektoren ind i CHO-cellelinjen, der er deponeret under nr. CCL-61 ved ATCC.
10. Kombination af: i) en kinesisk hamster-ovarie (CHO)-cellelinje; og ii) en DNA (deoxyribonukleinsyre)-vektor, der er egnet til produktion af et rekombinant protein, hvor vektoren omfatter en sekvens, der koder for en hererolog mammalia-glutaminsyntetase (GS) under kontrol af fra en promotor fra vakuolerende simianvirus 40 (SV40), herunder SV40-enhanceren, hvor GS omfatter en sekvens, der er mindst 94,5 % identisk med sekvensen ifølge SEQ ID NO: 1 eller med sekvensen ifølge SEQ ID NO: 2.
11. Kit, der omfatter kombinationen ifølge krav 10.
12. In vitro-fremgangsmåde til produktion af et rekombinant protein, der omfatter trinene: a) tilvejebringelse af en CHO-cellelinje ifølge et hvilket som helst af kravene 1 til 9; b) dyrkning af den opnåede CHO-cellelinje under betingelser, der er egnet til produktion af det rekombinante protein; og c) isolering og/eller oprensning af det rekombinante protein.
13. Fremgangsmåde ifølge krav 12, der yderligere omfatter trinet formulering af det rekombinante protein i en farmaceutisk sammensætning.
14. Anvendelse af CHO-cellelinjen ifølge et hvilket som helst af kravene 1 til 9, eller af kombinationen ifølge krav 10 til produktion af et rekombinant protein in vitro.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12305677.2A EP2674495A1 (en) | 2012-06-14 | 2012-06-14 | CHO expression system |
| PCT/EP2013/062400 WO2013186371A1 (en) | 2012-06-14 | 2013-06-14 | Cho expression system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2861741T3 true DK2861741T3 (da) | 2017-07-17 |
Family
ID=48700539
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK13732112.1T DK2861741T3 (da) | 2012-06-14 | 2013-06-14 | Cho-ekspressionssystem |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US9334489B2 (da) |
| EP (2) | EP2674495A1 (da) |
| CN (2) | CN107881151A (da) |
| AR (1) | AR091418A1 (da) |
| AU (1) | AU2013276467B2 (da) |
| CA (1) | CA2876550C (da) |
| DK (1) | DK2861741T3 (da) |
| ES (1) | ES2627954T3 (da) |
| HU (1) | HUE032968T2 (da) |
| IN (1) | IN2014KN02823A (da) |
| MX (1) | MX353499B (da) |
| SG (1) | SG11201408051VA (da) |
| TW (1) | TWI605123B (da) |
| UY (1) | UY34860A (da) |
| WO (1) | WO2013186371A1 (da) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AR102333A1 (es) | 2014-08-19 | 2017-02-22 | Regeneron Pharma | Selectividad eficiente de proteínas recombinadas |
| EP3209770B1 (en) * | 2014-10-23 | 2019-11-27 | Sanofi | Novel selection marker for cell transfection and protein production |
| US11098310B2 (en) | 2016-01-27 | 2021-08-24 | Just-Evotec Biologics, Inc. | Expression from transposon-based vectors and uses |
| WO2017132376A1 (en) | 2016-01-27 | 2017-08-03 | Just Biotherapeutics, Inc. | Hybrid promoter and uses thereof |
| US11261462B2 (en) | 2016-01-27 | 2022-03-01 | Just-Evotec Biologics, Inc. | Inducible expression from transposon-based vectors and uses |
| JP2020501506A (ja) | 2016-09-07 | 2020-01-23 | イッサム リサーチ デベロップメント カンパニー オブ ザ ヘブリュー ユニバーシティー オブ エルサレム エルティーディー． | 抗ＮＫｐ４６抗体およびその治療的使用 |
| KR20200018488A (ko) | 2017-05-24 | 2020-02-19 | 토리스 게엠베하 | 고암모니아혈증을 치료하기 위한 글루타민 합성효소의 용도 |
| IL253984A0 (en) | 2017-08-14 | 2017-09-28 | Yeda Res & Dev | Immunotherapy against aranviruses that bind through transferrin receptor 1 (tfr1 |
| CN108085337B (zh) * | 2017-12-28 | 2020-10-16 | 未名生物医药有限公司 | 一种gs表达系统细胞株的筛选方法 |
| CN109988777A (zh) * | 2017-12-29 | 2019-07-09 | 南京金斯瑞生物科技有限公司 | 谷氨酰胺合成酶基因以及应用 |
| US20220356487A1 (en) * | 2019-06-07 | 2022-11-10 | Biocon Biologics India Limited | Mammalian expression vectors |
| IL276627A (en) | 2020-08-10 | 2022-03-01 | Yeda Res & Dev | Compositions for diagnosis and treatment of coronavirus infections |
| TW202227622A (zh) | 2020-09-21 | 2022-07-16 | 法商賽諾菲公司 | 用於生產治療蛋白之高生產重組中國倉鼠卵巢細胞株之世代 |
| EP4384623A1 (en) * | 2021-08-03 | 2024-06-19 | Shanghai Zhenge Biotechnology Co., Ltd. | Selectable markers for eukaryotic expression system |
| WO2023125389A1 (en) * | 2021-12-27 | 2023-07-06 | Shanghai Zhenge Biotechnology Co., Ltd. | Novel markers for recombinant production system |
| CN115287300A (zh) * | 2022-08-11 | 2022-11-04 | 无锡多宁生物科技有限公司 | 一种增强ExpiCHO细胞瞬时转染抗体表达的方法 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8601597D0 (en) | 1986-01-23 | 1986-02-26 | Wilson R H | Nucleotide sequences |
| GB8717430D0 (en) | 1987-07-23 | 1987-08-26 | Celltech Ltd | Recombinant dna product |
| GB8924021D0 (en) * | 1989-10-25 | 1989-12-13 | Celltech Ltd | Recombinant dna method and vectors for the use therein |
| KR100267720B1 (ko) * | 1997-12-29 | 2001-03-02 | 박호군 | 사람 글루타민 합성효소 유전자를 포함하는 발현벡터 및 이를 이용하여 동물세포에서 에리트로포이에틴을 고수율로 생산하는 방법 |
| GB0216648D0 (en) * | 2002-07-18 | 2002-08-28 | Lonza Biologics Plc | Method of expressing recombinant protein in CHO cells |
| EP2316487B1 (en) * | 2003-04-11 | 2014-06-11 | MedImmune, LLC | Recombinant IL-9 antibodies & uses thereof |
| US7244616B2 (en) * | 2003-06-27 | 2007-07-17 | Bayer Pharmaceuticals Corporation | Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells |
| CN101875692A (zh) * | 2004-12-30 | 2010-11-03 | 新加坡科技研究局 | 中国仓鼠凋亡相关基因 |
| GB0508154D0 (en) * | 2005-04-22 | 2005-06-01 | Lonza Biologics Plc | Mammalian expression vector comprising the mCMV promoter and first intron of hCMV major immediate early gene |
| GB0510277D0 (en) * | 2005-05-20 | 2005-06-29 | Lonza Biologics Plc | High-level expression of recombinant antibody in a mammalian host cell |
| CN101627123A (zh) * | 2007-01-08 | 2010-01-13 | 米利波尔公司 | 消除基因扩增的高表达细胞系 |
| MX2010003622A (es) * | 2007-10-12 | 2010-04-14 | Hoffmann La Roche | Expresion proteinica a partir de acidos nucleicos multiples. |
| HUE033825T2 (en) | 2007-11-16 | 2018-01-29 | Univ Rockefeller | Beta-amyloid protein protofibrillum-specific antibodies |
-
2012
- 2012-06-14 EP EP12305677.2A patent/EP2674495A1/en not_active Withdrawn
-
2013
- 2013-06-12 AR ARP130102062 patent/AR091418A1/es unknown
- 2013-06-14 WO PCT/EP2013/062400 patent/WO2013186371A1/en active Application Filing
- 2013-06-14 MX MX2014015280A patent/MX353499B/es active IP Right Grant
- 2013-06-14 AU AU2013276467A patent/AU2013276467B2/en active Active
- 2013-06-14 IN IN2823KON2014 patent/IN2014KN02823A/en unknown
- 2013-06-14 CN CN201711180397.7A patent/CN107881151A/zh active Pending
- 2013-06-14 SG SG11201408051VA patent/SG11201408051VA/en unknown
- 2013-06-14 TW TW102121230A patent/TWI605123B/zh active
- 2013-06-14 UY UY0001034860A patent/UY34860A/es not_active Application Discontinuation
- 2013-06-14 ES ES13732112.1T patent/ES2627954T3/es active Active
- 2013-06-14 EP EP13732112.1A patent/EP2861741B1/en active Active
- 2013-06-14 HU HUE13732112A patent/HUE032968T2/en unknown
- 2013-06-14 DK DK13732112.1T patent/DK2861741T3/da active
- 2013-06-14 US US14/407,836 patent/US9334489B2/en active Active
- 2013-06-14 CN CN201380042643.6A patent/CN104540950A/zh active Pending
- 2013-06-14 CA CA2876550A patent/CA2876550C/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2013186371A1 (en) | 2013-12-19 |
| CA2876550C (en) | 2021-07-13 |
| CN107881151A (zh) | 2018-04-06 |
| US9334489B2 (en) | 2016-05-10 |
| MX2014015280A (es) | 2015-06-23 |
| EP2674495A1 (en) | 2013-12-18 |
| TW201402817A (zh) | 2014-01-16 |
| EP2861741B1 (en) | 2017-04-12 |
| UY34860A (es) | 2014-01-31 |
| TWI605123B (zh) | 2017-11-11 |
| IN2014KN02823A (da) | 2015-05-08 |
| US20150140607A1 (en) | 2015-05-21 |
| EP2861741A1 (en) | 2015-04-22 |
| AU2013276467B2 (en) | 2018-08-30 |
| CN104540950A (zh) | 2015-04-22 |
| AR091418A1 (es) | 2015-02-04 |
| CA2876550A1 (en) | 2013-12-19 |
| SG11201408051VA (en) | 2015-01-29 |
| ES2627954T3 (es) | 2017-08-01 |
| AU2013276467A1 (en) | 2015-01-15 |
| MX353499B (es) | 2018-01-16 |
| HUE032968T2 (en) | 2017-11-28 |
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