DK2744904T3 - Rekombinante mikroorganismer til fremstilling af C4-dicarboxylsyrer - Google Patents

Rekombinante mikroorganismer til fremstilling af C4-dicarboxylsyrer Download PDF

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DK2744904T3
DK2744904T3 DK12753881.7T DK12753881T DK2744904T3 DK 2744904 T3 DK2744904 T3 DK 2744904T3 DK 12753881 T DK12753881 T DK 12753881T DK 2744904 T3 DK2744904 T3 DK 2744904T3
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DK12753881.7T
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Sarah Mcfarland
Stephen Brown
Sheryl Luttringer
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Novozymes Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
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  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (15)

1. Rekombinant værtscelle omfattende et heterologt polynukleotid, som koder for et polypeptid med carboanhydraseaktivitet, hvor værtscellen er i stand til at producere en større mængde C4-dicarboxylsyre sammenlignet med værtscellen uden det heterologe polynukleotid, når dyrkning sker under de samme betingelser; hvor den rekombinante værtscelle er en svampeværtscelle; og hvor det heterologe polynukleotid: (a) koder for en carboanhydrase med i det mindste 80% sekvensidentitet med SEQ ID NR: 55; (b) under middel-stringensbetingelser hybridiserer med (i) den fulde længe af den komplementære streng i SEQ ID NR: 54, eller (ii) den fulde længe af den komplementære streng af cDNA sekvensen i SEQ ID NR: 54; or (c) har i det mindste 80% sekvensidentitet med SEQ ID NR: 54 eller cDNA sekvensen i SEQ ID NR: 54.
2. Rekombinant værtscelle ifølge krav 1, hvor det heterologe polynukleotid koder for en carboanhydrase med i det mindste 90% sekvensidentitet med SEQ ID NR: 55.
3. Rekombinant værtscelle ifølge krav 1 eller 2, hvor det heterologe polynukleotid koder for en carboanhydrasevariant af SEQ ID NR: 55, hvor varianten omfatter en substitution, deletion, og/eller insertion af ikke flere end 10 positioner.
4. Rekombinant værtscelle ifølge ethvert af kravene 1-3, hvor det heterologe polynukleotid koder for en carboanhydrase, som omfatter eller består af SEQ ID NR: 55.
5. Rekombinant værtscelle ifølge ethvert af kravene 1-4, som endvidere omfatter et heterologt polynukleotid, der koder for en bicarbonattransporter.
6. Rekombinant værtscelle ifølge ethvert af kravene 1-5, som endvidere omfatter et heterologt polynukleotid, der koder for en C4-dicarboxylsyretransporter.
7. Rekombinant værtscelle ifølge ethvert af kravene 1-6, som endvidere omfatter et heterologt polynukleotid, der koder for en malatdehydrogenase.
8. Rekombinant værtscelle ifølge ethvert af kravene 1-7, som endvidere omfatter et heterologt polynukleotid, der koder for en pyruvatcarboxylase.
9. Rekombinant værtscelle ifølge ethvert af kravene 1-8, hvor værtscellen er en filamentøs svampeværtscelle.
10. Rekombinant værtscelle ifølge krav 9, hvor værtscellen er valgt fra gruppen bestående af en Acremonium, Aspergillus, Aureobasidium, Bjerkandera, Ceriporiopsis, Chrysosporium, Coprinus, Coriolus, Cryptococcus, Filibasidium, Fusarium, Humicola, Magnaporthe, Mucor, Myceliophthora, Neocallimastix, Neurospora, Paecilomyces, Penicillium, Phanerochaete, Phlebia, Piromyces, Pleurotus, Rhizopus, Schizophyllum, Talaromyces, Thermoascus, Thielavia, Tolypocladium, Trametes og Trichoderma.
11. Rekombinant værtscelle ifølge krav 10, hvor værtscellen er en Aspergillus værtscelle, som for eksempel en Aspergillus oryzae-værtscelle.
12. Rekombinant værtscelle ifølge ethvert af kravene 1-11, hvor C4-dicarboxylsyren er en æblesyre.
13. Rekombinant værtscelle ifølge ethvert af kravene 1-12, hvor værtscellen er i stand til at producere en større mængde C4-dicarboxylsyre med i det mindste 10%, fortrinsvis med i det mindste 50%, sammenlignet med værtscellen uden det heterologe polynukleotid, der koder for carboanhydrasen, når dyrkning sker under de samme betingelser.
14. Fremgangsmåde til fremstilling af en C4-dicarboxylsyre, omfattende: (a) dyrkning af den rekombinante værtscelle ifølge ethvert af kravene 1-13 i et medie under passende betingelser til at producere C4-dicarboxylsyren; og (b) indvinding af C4-dicarboxylsyren.
15. Fremgangsmåde ifølge krav 14, hvor C4-dicarboxylsyren er en æblesyre.
DK12753881.7T 2011-08-19 2012-08-17 Rekombinante mikroorganismer til fremstilling af C4-dicarboxylsyrer DK2744904T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161525345P 2011-08-19 2011-08-19
PCT/US2012/051331 WO2013028512A1 (en) 2011-08-19 2012-08-17 Recombinant microorganisms for production c4-dicarboxylic acids

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DK2744904T3 true DK2744904T3 (da) 2016-07-25

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US (1) US8663956B2 (da)
EP (1) EP2744904B1 (da)
CN (1) CN103917649A (da)
BR (1) BR112014003444A2 (da)
DK (1) DK2744904T3 (da)
ES (1) ES2579706T3 (da)
WO (1) WO2013028512A1 (da)

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US10385368B2 (en) * 2014-06-26 2019-08-20 Lygos, Inc. Recombinant host cells for the production of malonate
CN106148208B (zh) * 2015-03-23 2020-12-18 中国科学院天津工业生物技术研究所 新的二元有机酸生产菌株及其制备和应用
CN106148209B (zh) * 2015-03-23 2020-11-20 中国科学院天津工业生物技术研究所 新的二元有机酸生产菌株及其制备和应用
CN109415720B (zh) 2016-06-29 2021-11-26 花王株式会社 C4二羧酸的制造方法
CN107574190B (zh) * 2017-07-24 2020-08-07 山东理工大学 一种提高卷枝毛霉产油量的制备方法
CN107988088B (zh) * 2017-12-15 2021-06-25 北京工商大学 一株沙漠毕赤酵母在水果采后病害防治中的应用
CN107937289B (zh) * 2017-12-15 2021-06-25 北京工商大学 一株盔状毕赤酵母by27菌株在水果采后病害防治中的应用
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CN114807193B (zh) * 2022-05-13 2023-11-21 厦门大学 一种碳酸酐酶基因及其用途
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US8663956B2 (en) 2014-03-04
CN103917649A (zh) 2014-07-09
EP2744904A1 (en) 2014-06-25
WO2013028512A1 (en) 2013-02-28
EP2744904B1 (en) 2016-04-13
ES2579706T3 (es) 2016-08-16
BR112014003444A2 (pt) 2017-03-14
US20130059353A1 (en) 2013-03-07

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