DK2736351T3 - Isolering af protein fra oliefrø - Google Patents
Isolering af protein fra oliefrø Download PDFInfo
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- DK2736351T3 DK2736351T3 DK12733111.4T DK12733111T DK2736351T3 DK 2736351 T3 DK2736351 T3 DK 2736351T3 DK 12733111 T DK12733111 T DK 12733111T DK 2736351 T3 DK2736351 T3 DK 2736351T3
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- DK
- Denmark
- Prior art keywords
- protein
- weight
- content
- dry matter
- ethanol
- Prior art date
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- 102000004169 proteins and genes Human genes 0.000 title claims description 172
- 108090000623 proteins and genes Proteins 0.000 title claims description 172
- 238000002955 isolation Methods 0.000 title description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 150
- 240000002791 Brassica napus Species 0.000 claims description 67
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 63
- 238000000034 method Methods 0.000 claims description 55
- 239000000203 mixture Substances 0.000 claims description 47
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 45
- 238000000605 extraction Methods 0.000 claims description 39
- 239000007864 aqueous solution Substances 0.000 claims description 30
- 239000003021 water soluble solvent Substances 0.000 claims description 30
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- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical class OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 claims description 20
- 235000002949 phytic acid Nutrition 0.000 claims description 20
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- 238000000108 ultra-filtration Methods 0.000 claims description 19
- PCMORTLOPMLEFB-ONEGZZNKSA-N sinapic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC(OC)=C1O PCMORTLOPMLEFB-ONEGZZNKSA-N 0.000 claims description 18
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- QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 description 1
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- HUJXHFRXWWGYQH-UHFFFAOYSA-O sinapine Chemical compound COC1=CC(\C=C\C(=O)OCC[N+](C)(C)C)=CC(OC)=C1O HUJXHFRXWWGYQH-UHFFFAOYSA-O 0.000 description 1
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- GCLGEJMYGQKIIW-UHFFFAOYSA-H sodium hexametaphosphate Chemical compound [Na]OP1(=O)OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])O1 GCLGEJMYGQKIIW-UHFFFAOYSA-H 0.000 description 1
- 235000019982 sodium hexametaphosphate Nutrition 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
- A23J1/142—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds by extracting with organic solvents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Peptides Or Proteins (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Fats And Perfumes (AREA)
Claims (15)
1. Fremgangsmåde til isolering af nativt protein fra oliefrømel eller oliefrø-oliekage, hvilken fremgangsmåde omfatter følgende trin: • ekstraktion af melet med vand for at opnå en vandig opløsning; • koncentrering af den vandige ekstrakt til en vandig opløsning, som omfatter 5 til 30 vægtprocent protein, fortrinsvis 10 til 30 vægtprocent protein; • tilsætning af et vandopløseligt opløsningsmiddel til den koncentrerede vandige opløsning for at opnå et proteinbundfald, hvorved det vandopløselige opløsningsmiddel er ethanol, og hvilket ethanol tilsættes til et endeligt indhold på mellem 60 og 80 volumenprocent; og • separation af proteinbundfaldet fra væskefraktionen.
2. Fremgangsmåde ifølge krav 1, som omfatter det yderligere trin til vask af proteinbundfaldet.
3. Fremgangsmåde ifølge krav 1 eller 2, som omfatter det yderligere trin til tørring af proteinbundfaldet.
4. Fremgangsmåde ifølge et hvilket som helst af kravene, hvorved den vandige opløsning eller koncentrerede vandige opløsning diafiltreres efter ekstraktionstrinet og før tilsætningen af det vandopløselige opløsningsmiddel, fortrinsvis ved anvendelse af UF (ultrafiltrering).
5. Fremgangsmåde ifølge krav 4, hvorved opløselige carbohydrater, glucosinolater eller derivater deraf, phytater eller polyphenolforbindelser eller en kombination af en eller flere af disse forbindelser fjernes fra den vandige opløsning eller koncentrerede vandige opløsning.
6. Fremgangsmåde ifølge krav 4 eller 5, hvorved diafiltreringen finder sted før, under eller efter koncentreringen af den vandige ekstrakt.
7. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor melet er raps-, soja- eller solsikkemel.
8. Isoleret nativt oliefrøprotein eller nativ oliefrøprotein-sammensætning, som omfatter et proteinindhold på mindst 80 vægtprocent, fortrinsvis mindst 85 vægtprocent, mere fortrinsvis mindst 90 vægtprocent og mest fortrinsvis mellem 92 og 99 vægtprocent (på tørstofbasis); - et ethanolindhold på mindre end 0,2 vægtprocent, fortrinsvis mindre end 0,1 vægtprocent (på tørstofbasis); - et ethanolindhold på mere end 0,001 vægtprocent, fortrinsvis mere end 0,01 vægtprocent (på tørstofbasis) og - et phenolindhold på mindre end 0,1 vægtprocent, fortrinsvis mindre end 0,05 vægtprocent, mere fortrinsvis mindre end 0,02 vægtprocent (på tørstofbasis), udtrykt som sinapinsyreækvivalenter.
9. Isoleret protein eller proteinsammensætning ifølge krav 8, som har et glucosinolatindhold på mindre end 10 pmol/g, fortrinsvis mindre end 1 ymol/g (på tørstofbasis).
10. Isoleret protein eller proteinsammensætning ifølge krav 8 eller 9, som har et lipidindhold på 2 til 15 vægtprocent, fortrinsvis 2 til 10 vægtprocent og mere fortrinsvis 2 til 8 vægtprocent (på tørstofbasis) eller 2 til 20 vægtprocent, fortrinsvis 2 til 15 vægtprocent (på tørstofbasis) i tilfælde af sojaprotein.
11. Isoleret protein eller proteinsammensætning ifølge et hvilket som helst af kravene 8-10, som har et phytatindhold (P x 3,5) på mindre end 0,5 vægtprocent, fortrinsvis mindre end 0,2 vægtprocent (på tørstofbasis).
12. Isoleret protein eller proteinsammensætning ifølge et hvilket som helst af kravene 8-11, som har en opløselighed på mindst 30 NS%, fortrinsvis mindst 50 NS%, mere fortrinsvis mindst 60 NS%, endnu mere fortrinsvis mindst 70 NS% og mest fortrinsvis mindst 75 NS%.
13. Isoleret protein eller proteinsammensætning ifølge et hvilket som helst af kravene 8-10, som har et tørstofindhold på mindst 70 vægtprocent, fortrinsvis mindst 80 vægtprocent, mere fortrinsvis mindst 85 vægtprocent, endnu mere fortrinsvis mindst 90 vægtprocent, meget mere fortrinsvis mindst 91 vægtprocent, endnu meget mere fortrinsvis har et tørstofindhold på 92 til 99 vægtprocent og mest fortrinsvis på 93 til 98 vægtprocent.
14. Isoleret protein eller proteinsammensætning ifølge et hvilket som helst af kravene 8-13, som omfatter raps-, solsikke- eller sojaprotein.
15. Isoleret protein eller proteinsammensætning ifølge et hvilket som helst af kravene 8-13, som omfatter raps, og hvor 2S-proteinet og 12S-proteinet vil være til stede i et forhold på 1:6 til 6:1, fortrinsvis i et forhold på 1:2 til 2:1 (vægt/vægt på tørstofbasis).
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11175743 | 2011-07-28 | ||
| PCT/EP2012/063134 WO2013013949A1 (en) | 2011-07-28 | 2012-07-05 | Protein isolation from oil seeds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2736351T3 true DK2736351T3 (da) | 2016-04-11 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12733111.4T DK2736351T3 (da) | 2011-07-28 | 2012-07-05 | Isolering af protein fra oliefrø |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20150073127A1 (da) |
| EP (1) | EP2736351B1 (da) |
| AU (1) | AU2012289115B2 (da) |
| CA (1) | CA2843177C (da) |
| DK (1) | DK2736351T3 (da) |
| EA (1) | EA026579B1 (da) |
| ES (1) | ES2565655T3 (da) |
| HU (1) | HUE026992T2 (da) |
| PL (1) | PL2736351T3 (da) |
| UA (1) | UA113181C2 (da) |
| WO (1) | WO2013013949A1 (da) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2014234432A1 (en) * | 2013-03-18 | 2015-09-17 | Dsm Ip Assets B.V. | Method for protein extraction from oil seed |
| WO2016042001A1 (en) | 2014-09-18 | 2016-03-24 | Dsm Ip Assets B.V. | Method for producing an oil seed protein mix |
| US10602756B2 (en) | 2014-12-11 | 2020-03-31 | NapiFeryn Bio Tech sp. z o.o | Mild fractionation of functional isolates derived from grains and oilseeds |
| CN108366579A (zh) | 2015-12-17 | 2018-08-03 | 帝斯曼知识产权资产管理有限公司 | 油菜籽蛋白质分离物、包含所述分离物的食物以及作为发泡剂或乳化剂的用途 |
| CA3026604A1 (en) * | 2016-07-07 | 2018-01-11 | Dsm Ip Assets B.V. | Food grade native rapeseed protein isolate and process for obtaining it |
| WO2018007493A1 (en) | 2016-07-07 | 2018-01-11 | Dsm Ip Assets B.V. | Rapeseed protein isolate, food comprising the isolate and use as foaming or emulsifying agent |
| PL3481220T3 (pl) * | 2016-07-07 | 2023-09-18 | Dsm Ip Assets B.V. | Słodki izolat protein rzepakowych i sposób jego otrzymywania |
| CA3026631C (en) | 2016-07-07 | 2024-03-26 | Dsm Ip Assets B.V. | Process for obtaining a rapeseed protein isolate and protein isolate thereby obtained |
| CN109788777B (zh) | 2016-07-07 | 2023-04-04 | 帝斯曼知识产权资产管理有限公司 | 包含油菜籽蛋白质分离物的乳剂 |
| EP3498103A1 (de) * | 2017-12-13 | 2019-06-19 | Drei Lilien Pvg GmbH&Co. KG | Verfahren zur prozessökonomischen ab-/auftrennung von konstituenten pflanzlicher ausgangsmaterialien sowie deren gewinnung und verwendung |
| MX2019011609A (es) | 2017-03-28 | 2020-01-30 | Dietz Max | Metodo de procesamiento economico de desconexion/desprendimiento de componentes de materias primas de origen vegetal, su obtencion y uso. |
| EP3651586B1 (en) * | 2017-07-10 | 2021-03-17 | Napiferyn Biotech Sp. Z O.o | Method for isolation of protein from plant material |
| PL422158A1 (pl) * | 2017-07-10 | 2019-01-14 | Napiferyn Biotech Spolka Z Ograniczona Odpowiedzialnoscia | Sposób izolacji białka z nasion roślin, izolat białkowy i zastosowanie izolatu białkowego |
| NL2019207B1 (en) * | 2017-07-10 | 2019-01-16 | Napiferyn Biotech Sp Z O O | Method for isolation of protein from plant material |
| US20220071230A1 (en) * | 2018-12-21 | 2022-03-10 | Botaneco Inc. | Plant protein concentrates |
| EP3735835A1 (en) * | 2019-05-10 | 2020-11-11 | Avril | Sunflower albumin isolate and process for the production thereof |
| EP3841886A1 (en) | 2019-12-23 | 2021-06-30 | Avril | Sunflower seed protein concentrate for food applications and method of manufacturing the same |
| EP3970505A1 (en) | 2020-09-17 | 2022-03-23 | Avril | A sunflower seed protein concentrate and process for the production thereof |
| NL2028223B1 (en) | 2021-05-17 | 2022-12-02 | Napiferyn Biotech Sp Z O O | Improved method for preparation of protein-enriched products from plant material |
| WO2023052590A1 (en) * | 2021-09-30 | 2023-04-06 | Dsm Ip Assets B.V. | Rapeseed protein isolate |
| WO2023147534A1 (en) * | 2022-01-31 | 2023-08-03 | Bunge Sa | Process for production of sunflower protein concentrate and sunflower protein flour |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58224645A (ja) * | 1982-06-23 | 1983-12-27 | Ichimaru Fuarukosu Kk | 納豆抽出物の製造法 |
| US6610831B1 (en) * | 1999-12-21 | 2003-08-26 | Lurgi Psi, Ltd. | Methods and apparatus for recovering zein from corn |
| NL1017241C2 (nl) * | 2001-01-30 | 2002-07-31 | Tno | Werkwijze voor het bereiden van een eiwitpreparaat met verlaagd gehalte aan fenolische verbindingen. |
| US8048463B2 (en) * | 2001-05-29 | 2011-11-01 | Levente Laszlo Diosady | Production of high-quality protein isolated from oil seeds |
| US6905713B2 (en) * | 2001-05-29 | 2005-06-14 | Levente Laszlo Diosady | Production of high-quality protein isolates from defatted meals of Brassica seeds |
| US20030109679A1 (en) * | 2001-10-10 | 2003-06-12 | Green Brent Everett | Flax protein isolate and production |
-
2012
- 2012-05-07 UA UAA201401675A patent/UA113181C2/uk unknown
- 2012-07-05 EP EP12733111.4A patent/EP2736351B1/en active Active
- 2012-07-05 WO PCT/EP2012/063134 patent/WO2013013949A1/en active Application Filing
- 2012-07-05 HU HUE12733111A patent/HUE026992T2/en unknown
- 2012-07-05 PL PL12733111T patent/PL2736351T3/pl unknown
- 2012-07-05 DK DK12733111.4T patent/DK2736351T3/da active
- 2012-07-05 AU AU2012289115A patent/AU2012289115B2/en active Active
- 2012-07-05 ES ES12733111.4T patent/ES2565655T3/es active Active
- 2012-07-05 EA EA201400177A patent/EA026579B1/ru not_active IP Right Cessation
- 2012-07-05 US US14/234,741 patent/US20150073127A1/en not_active Abandoned
- 2012-07-05 CA CA2843177A patent/CA2843177C/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CA2843177C (en) | 2019-04-09 |
| ES2565655T3 (es) | 2016-04-06 |
| CA2843177A1 (en) | 2013-01-31 |
| US20150073127A1 (en) | 2015-03-12 |
| EA201400177A1 (ru) | 2014-05-30 |
| PL2736351T3 (pl) | 2016-06-30 |
| CN103732076A (zh) | 2014-04-16 |
| AU2012289115A1 (en) | 2014-02-06 |
| EA026579B1 (ru) | 2017-04-28 |
| NZ620272A (en) | 2016-02-26 |
| WO2013013949A1 (en) | 2013-01-31 |
| HUE026992T2 (en) | 2016-08-29 |
| EP2736351A1 (en) | 2014-06-04 |
| EP2736351B1 (en) | 2016-01-06 |
| UA113181C2 (xx) | 2016-12-26 |
| AU2012289115B2 (en) | 2016-02-25 |
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