DK2661492T3 - Fremgangsmåde til fremstilling af rekombinante glykoproteiner med forhøjet kredsløbshalveringstid i pattedyrceller - Google Patents

Fremgangsmåde til fremstilling af rekombinante glykoproteiner med forhøjet kredsløbshalveringstid i pattedyrceller Download PDF

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Publication number
DK2661492T3
DK2661492T3 DK12731900.2T DK12731900T DK2661492T3 DK 2661492 T3 DK2661492 T3 DK 2661492T3 DK 12731900 T DK12731900 T DK 12731900T DK 2661492 T3 DK2661492 T3 DK 2661492T3
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Denmark
Prior art keywords
alpha2
rhubche
cho
sialic acid
bche
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DK12731900.2T
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English (en)
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Michael J Betenbaugh
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Univ Johns Hopkins
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01214—Glycoprotein 3-alpha-L-fucosyltransferase (2.4.1.214)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99001—Beta-galactoside alpha-2,6-sialyltransferase (2.4.99.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01008—Cholinesterase (3.1.1.8), i.e. butyrylcholine-esterase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (4)

1. En isoleret pattedyrcelle omfattende en heterolog alpha2-6-sialy!transferase nukie-insyresekvens, eventuelt hvor cellen er en CHO-celle; yderligere omfattende en nukle-insyresekvens som reducerer ekspression af eller inaktivering af et alpha2-Ssialyltransferasegen; eller yderligere omfattende midlerne til knock-out af alpha2-3 sialyltransferasegenet,
2. Celle ifølge krav 1, hvor nukleinsyresekvensen som reducerer ekspression af eller inaktivering af alpha2-3sialyltransferasegenet eller midlerne til knock-out af alpha2-3 sialyltransferasegenet er udvalgt blandt gruppen bestående af antisense, siRNA, miRNA og en zinkfinger nuklease.
3. Celle ifølge krav 1 eller 2, hvor alpha2-3sialytransferasegenet er udvalgt blandt St3gall St3gal2, St3gal3, St3gal4, St3gal5, St3gal6-genet og en kombination deraf.
4. Fremgangsmåde til biosyntese af et alfa2-6-rigt glykoprotein omfattende dyrkning af en celle ifølge krav 1 under betingelser for at co-ekspiimere en nukleinsyresekvens som koder for et peptid eller protein, yderligere omfatter inhibering af ekspression af alpha2-3-sialyltransferase.
DK12731900.2T 2011-01-06 2012-01-06 Fremgangsmåde til fremstilling af rekombinante glykoproteiner med forhøjet kredsløbshalveringstid i pattedyrceller DK2661492T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161430256P 2011-01-06 2011-01-06
PCT/US2012/020535 WO2012094627A2 (en) 2011-01-06 2012-01-06 Method of production of recombinant glycoproteins with increased circulatory half-life in mammalian cells

Publications (1)

Publication Number Publication Date
DK2661492T3 true DK2661492T3 (da) 2018-01-15

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ID=46457991

Family Applications (1)

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DK12731900.2T DK2661492T3 (da) 2011-01-06 2012-01-06 Fremgangsmåde til fremstilling af rekombinante glykoproteiner med forhøjet kredsløbshalveringstid i pattedyrceller

Country Status (10)

Country Link
US (2) US20130243744A1 (da)
EP (1) EP2661492B1 (da)
CN (2) CN107267459A (da)
AU (1) AU2012204241A1 (da)
BR (1) BR112013016447A2 (da)
CA (1) CA2817765A1 (da)
DK (1) DK2661492T3 (da)
MX (1) MX2013006234A (da)
SG (1) SG190260A1 (da)
WO (1) WO2012094627A2 (da)

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KR102212865B1 (ko) 2012-01-11 2021-02-05 시그마-알드리치 컴퍼니., 엘엘씨 단순한 글리코형을 갖는 재조합 단백질의 생산
CN106661569B (zh) * 2014-03-04 2020-09-11 西格马—奥尔德里奇有限责任公司 抗病毒细胞及其用途
US10221402B2 (en) 2014-03-16 2019-03-05 Plantvax, Inc. Production of highly thermally stable recombinant cholinesterases for the detection, detoxification and decontamination of organophosphorus compounds
WO2015196150A2 (en) 2014-06-20 2015-12-23 Wisconsin Alumni Research Foundation (Warf) Mutations that confer genetic stability to additional genes in influenza viruses
DK3218005T3 (da) 2014-11-12 2023-03-27 Seagen Inc Glycan-interagerende forbindelser og anvendelsesfremgangsmåder
MA41116A (fr) * 2014-12-01 2017-10-10 Ferring Bv Compositions d'inhibiteur de trans-signalisation par l'il-6 sélectif
EP3912636A1 (en) 2014-12-01 2021-11-24 Ferring B.V. Administration of a selective il-6-trans-signalling inhibitor
WO2016091268A2 (en) * 2014-12-12 2016-06-16 University Of Copenhagen N-glycosylation
EP3042952A1 (en) * 2015-01-07 2016-07-13 CEVEC Pharmaceuticals GmbH O-glycan sialylated recombinant glycoproteins and cell lines for producing the same
KR20180088381A (ko) * 2015-11-12 2018-08-03 시아맙 쎄라퓨틱스, 인코포레이티드 글리칸-상호작용 화합물 및 사용방법
EP3512876B1 (en) * 2016-09-13 2021-10-27 Coagulant Therapeutics Corporation Factor viia glycoforms
EP3541847A4 (en) 2016-11-17 2020-07-08 Seattle Genetics, Inc. COMPOUNDS INTERACTING WITH GLYCANE AND METHODS OF USE
AU2018226824A1 (en) 2017-03-03 2019-09-19 Seagen Inc. Glycan-interacting compounds and methods of use
JP2020511499A (ja) * 2017-03-20 2020-04-16 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 赤血球生成刺激タンパク質のインビトロでの糖鎖改変のための方法
CN108659095A (zh) * 2018-05-18 2018-10-16 上海药明生物技术有限公司 一种使唾液酸含量稳定的方法
WO2020163804A1 (en) * 2019-02-08 2020-08-13 Wisconsin Alumni Research Foundation (Warf) Humanized cell line
JP2022551805A (ja) 2019-08-27 2022-12-14 ウィスコンシン アルムニ リサーチ ファンデイション 卵内複製のための安定化されたｈａを持つ組換えインフルエンザウイルス
CN117587020B (zh) * 2024-01-18 2024-04-02 中国人民解放军东部战区总医院 抑制ST3GAL1基因表达的shRNA及其应用

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US6204431B1 (en) * 1994-03-09 2001-03-20 Abbott Laboratories Transgenic non-human mammals expressing heterologous glycosyltransferase DNA sequences produce oligosaccharides and glycoproteins in their milk
US20020045207A1 (en) * 1997-10-31 2002-04-18 Lynne A. Krummen Glycoprotein production process
DK1578771T3 (da) * 2001-10-10 2013-06-10 Novo Nordisk As Remodellering og glycokonjugering af peptider
AU2002365933A1 (en) * 2001-11-14 2003-09-02 Joseph D. Mosca Production of recombinant therapeutic bioscavengers against chemical and biological agents
PT1465987E (pt) * 2001-12-07 2008-04-15 Crucell Holland Bv Produção de vírus, isolados virais e vacinas
US20070111279A1 (en) * 2002-11-13 2007-05-17 Procell, Inc. Production of recombinant therapeutic bioscavengers against chemical and biological agents
BRPI0720861A2 (pt) * 2006-12-28 2014-02-25 Centocor Ortho Biotech Inc Métodos e vetores para gerar imunoglobulinas asialiladas
AU2008310583A1 (en) * 2007-10-12 2009-04-16 Sigma-Aldrich Co. Llc Compositions and methods for improved glycoprotein sialylation
BRPI0924078A2 (pt) * 2009-01-22 2018-10-16 Momenta Pharmaceuticals Inc n-glicanos contendo galactose-alfa-1,3-galactose em produtos de glicoproteína derivados de células de cho.

Also Published As

Publication number Publication date
EP2661492A4 (en) 2015-04-15
CN107267459A (zh) 2017-10-20
BR112013016447A2 (pt) 2019-09-24
MX2013006234A (es) 2013-08-01
EP2661492B1 (en) 2017-10-04
CA2817765A1 (en) 2012-07-12
US20130243744A1 (en) 2013-09-19
US20170159095A1 (en) 2017-06-08
CN103221537A (zh) 2013-07-24
AU2012204241A1 (en) 2013-06-06
WO2012094627A2 (en) 2012-07-12
WO2012094627A3 (en) 2012-10-04
SG190260A1 (en) 2013-06-28
EP2661492A2 (en) 2013-11-13

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