DK2398817T3 - Fremgangsmåde til rensning af lipopeptider - Google Patents
Fremgangsmåde til rensning af lipopeptider Download PDFInfo
- Publication number
- DK2398817T3 DK2398817T3 DK10707694.5T DK10707694T DK2398817T3 DK 2398817 T3 DK2398817 T3 DK 2398817T3 DK 10707694 T DK10707694 T DK 10707694T DK 2398817 T3 DK2398817 T3 DK 2398817T3
- Authority
- DK
- Denmark
- Prior art keywords
- daptomycin
- solution
- reverse phase
- elution buffer
- phase chromatography
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
- C12P17/188—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/64—Cyclic peptides containing only normal peptide links
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Claims (20)
1. Fremgangsmåde til rensning af daptomycin omfattende trinene a) påfyldning af en opløsning indeholdende delvis renset daptomycin på en an-i o n bytte rkro matog raf i sø j I e; b) påfyldning af opløsningen fra trin a) på en omvendt fase-kromatografisøjle; c) eventuelt påfyldning af opløsningen fra trin b) på endnu en omvendt fase-kro-matografisøjle mindst én gang; hvor elueringsbufferen i a) er en monovalent saltopløsning, og elueringsbufferen i b) er vandig alkohol.
2. Fremgangsmåde ifølge krav 1, ved hvilken der udføres ét eller flere klaringstrin forud for anionbytterkromatografitrinet a).
3. Fremgangsmåde ifølge krav 1 - 2, ved hvilken produktet fra omvendt fase-kromatografitrinet b) renses yderligere ved ét eller flere filtreringstrin.
4. Fremgangsmåde ifølge krav 3, ved hvilken produktet fra den omvendte fase yderligere følges af et frysetørringstrin.
5. Fremgangsmåde ifølge krav 1, ved hvilken elueringsopløsningen i a) er NaCI.
6. Fremgangsmåde ifølge krav 2, ved hvilken elueringsbufferen er 0,1-1,5 M NaCI.
7. Fremgangsmåde ifølge krav 1, ved hvilken elueringsbufferen i b) er vandig ethanol.
8. Fremgangsmåde ifølge krav 7, ved hvilken elueringsbufferen er 5-80% ethanol.
9. Fremgangsmåde ifølge krav 1, ved hvilken anionbytterkromatografitrinet udføres under anvendelse af en stærkt tværbundet agaroseharpiks med dextran-overfladeextender.
10. Fremgangsmåde ifølge krav 1, ved hvilken omvendt fase-kromatografitrinet udføres under anvendelse af en styren-baseret harpikssøjle.
11. Fremgangsmåde ifølge krav 1, ved hvilken trin b) gentages én gang.
12. Fremgangsmåde ifølge krav 11, ved hvilken den første omvendt fase-kro-matografisøjle elueres ved en pFI-værdi på 6,5-8,5.
13. Fremgangsmåde ifølge krav 11, ved hvilken den anden omvendt fase-kro-matografisøjle elueres ved en pFI-værdi på 2,5-3,5.
14. Fremgangsmåde til rensning af daptomycin omfattende trinene a) udsættelse af en fermenteringsvæske indeholdende daptomycin for ét eller flere klaringstrin; b) påfyldning af opløsningen fra trin a) på en anionbytterkromatografisøjle; c) påfyldning af opløsningen fra trin b) på en omvendt fase-kromatografisøjle; d) eventuelt påfyldning af opløsningen fra trin c) på endnu en omvendt fase-kromatografisøjle mindst én gang; e) udsættelse af den resulterende opløsning fra trin d) for ét eller flere filtreringstrin; f) udsættelse af filtratet fra d) for frysetørring; resulterende i et renset pulver af daptomycin; hvor elueringsbufferen i trin c) og eventuelt i trin d) er vandig alkohol.
15. Fremgangsmåde ifølge krav 14, ved hvilken klaringstrinene i a) er én af eller en kombination af de følgende metoder: omvendt osmose, centrifugering, filtrering, ultrafiltrering, nanofiltrering, anionbytterkromatografi.
16. Fremgangsmåde ifølge krav 14, ved hvilken elueringsopløsningen i b) er NaCI.
17. Fremgangsmåde ifølge krav 16, ved hvilken elueringsbufferen er 0,1-1,5 M NaCI.
18. Fremgangsmåde ifølge krav 14, ved hvilken elueringsbufferen i c) er vandig ethanol.
19. Fremgangsmåde ifølge krav 18, ved hvilken elueringsbufferen er 5-80% ethanol.
20. Fremgangsmåde ifølge krav 14, ved hvilken trin c) gentages én gang.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15366009P | 2009-02-19 | 2009-02-19 | |
| PCT/NO2010/000066 WO2010095953A1 (en) | 2009-02-19 | 2010-02-19 | Process for purifying lipopeptides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK2398817T3 true DK2398817T3 (da) | 2015-07-27 |
| DK2398817T4 DK2398817T4 (da) | 2021-08-23 |
Family
ID=42116034
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK10707694.5T DK2398817T4 (da) | 2009-02-19 | 2010-02-19 | Fremgangsmåde til rensning af lipopeptider |
Country Status (21)
| Country | Link |
|---|---|
| US (1) | US9150894B2 (da) |
| EP (1) | EP2398817B2 (da) |
| JP (1) | JP5752609B2 (da) |
| KR (1) | KR101666144B1 (da) |
| CN (1) | CN102325785B (da) |
| AU (1) | AU2010216475B2 (da) |
| BR (1) | BRPI1008300B1 (da) |
| CA (1) | CA2748932C (da) |
| DK (1) | DK2398817T4 (da) |
| ES (1) | ES2543457T5 (da) |
| HR (1) | HRP20150761T4 (da) |
| HU (1) | HUE026818T2 (da) |
| IL (1) | IL214374A (da) |
| MX (1) | MX2011008621A (da) |
| PL (1) | PL2398817T5 (da) |
| PT (1) | PT2398817E (da) |
| RU (1) | RU2526391C2 (da) |
| SI (1) | SI2398817T2 (da) |
| UA (1) | UA105785C2 (da) |
| WO (1) | WO2010095953A1 (da) |
| ZA (1) | ZA201104848B (da) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9150894B2 (en) | 2009-02-19 | 2015-10-06 | Xellia Pharmaceuticals Aps | Process for purifying lipopeptides |
| CN102690329B (zh) * | 2011-03-25 | 2014-06-04 | 杭州九源基因工程有限公司 | 一种戈舍瑞林多肽的纯化生产方法 |
| CN102875652A (zh) * | 2011-07-13 | 2013-01-16 | 北大方正集团有限公司 | 一种分离纯化达托霉素的方法 |
| CN103159829B (zh) * | 2011-12-08 | 2014-11-05 | 北大方正集团有限公司 | 达托霉素的提取方法 |
| CN102675426B (zh) * | 2012-04-26 | 2013-12-11 | 杭州华东医药集团生物工程研究所有限公司 | 一种达托霉素的提取纯化方法 |
| CN102924572B (zh) * | 2012-11-12 | 2014-12-03 | 华北制药集团新药研究开发有限责任公司 | 一种高纯度达托霉素的制备方法 |
| CN104387444B (zh) * | 2014-11-13 | 2017-12-08 | 北大医药重庆大新药业股份有限公司 | 一种达托霉素杂质rs‑2高纯度样品的制备方法 |
| CA2970732C (en) * | 2014-12-15 | 2023-05-16 | Merck Patent Gmbh | Target molecule capture from crude solutions |
| CN105001305A (zh) * | 2015-04-29 | 2015-10-28 | 利穗科技(苏州)有限公司 | 利用层析技术提取高纯度达托霉素的方法 |
| CN105481950B (zh) * | 2016-01-28 | 2019-01-04 | 丽珠集团福州福兴医药有限公司 | 一种达托霉素提取方法 |
| IT201600127655A1 (it) | 2016-12-16 | 2018-06-16 | Gnosis Spa | Processo per la purificazione di antibiotici lipopolipeptidici |
| CN108250270A (zh) * | 2016-12-29 | 2018-07-06 | 天津领世生物科技开发有限公司 | 一种从发酵液中富集提取达托霉素的方法 |
| CN112979756B (zh) * | 2019-12-13 | 2023-01-03 | 浙江昌海制药有限公司 | 达托霉素的提纯方法 |
| CN113004373A (zh) * | 2019-12-19 | 2021-06-22 | 鲁南制药集团股份有限公司 | 一种达托霉素的纯化方法 |
| RU2762182C1 (ru) * | 2020-12-08 | 2021-12-16 | Федеральное государственное бюджетное научное учреждение "Научно-исследовательский институт по изысканию новых антибиотиков имени Г.Ф. Гаузе" | Гауземицины А и В - гликолипопептидные антибактериальные антибиотики и способ их получения |
| CN113340691B (zh) * | 2021-06-05 | 2023-12-12 | 清华大学 | 一种提取海洋沉积物中不同赋存形态磷的方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4537717A (en) | 1982-05-21 | 1985-08-27 | Eli Lilly And Company | Derivatives of A-21978C cyclic peptides |
| US4885243A (en) | 1984-10-09 | 1989-12-05 | Eli Lilly And Company | Process for producing A-21978C derivatives |
| US4667016A (en) | 1985-06-20 | 1987-05-19 | Kirin-Amgen, Inc. | Erythropoietin purification |
| CA1315229C (en) | 1987-06-10 | 1993-03-30 | Patrick J. Baker | Chromatographic purification process |
| US4874843A (en) | 1987-12-03 | 1989-10-17 | Eli Lilly And Company | Chromatographic purification process |
| JP2661790B2 (ja) * | 1990-11-27 | 1997-10-08 | 電気化学工業株式会社 | 入尿トリプシンインヒビターの精製方法 |
| CN1057534C (zh) * | 1993-08-17 | 2000-10-18 | 柯瑞英-艾格公司 | 促红细胞生成素类似物 |
| US5650496A (en) | 1995-04-14 | 1997-07-22 | Cephalon, Inc. | IGF-I purification process |
| US6696412B1 (en) * | 2000-01-20 | 2004-02-24 | Cubist Pharmaceuticals, Inc. | High purity lipopeptides, Lipopeptide micelles and processes for preparing same |
| US20030045678A1 (en) | 2000-12-18 | 2003-03-06 | Dennis Keith | Methods for preparing purified lipopeptides |
| US20060014674A1 (en) | 2000-12-18 | 2006-01-19 | Dennis Keith | Methods for preparing purified lipopeptides |
| WO2008057683A2 (en) * | 2006-10-03 | 2008-05-15 | Novo Nordisk A/S | Methods for the purification of polypeptide conjugates |
| WO2009144739A1 (en) | 2008-05-26 | 2009-12-03 | Biocon Limited | Amorphous daptomycin and a method of purification thereof |
| US9150894B2 (en) | 2009-02-19 | 2015-10-06 | Xellia Pharmaceuticals Aps | Process for purifying lipopeptides |
| CN101899094B (zh) * | 2009-06-01 | 2012-11-21 | 安徽丰原发酵技术工程研究有限公司 | 一种高纯达托霉素的制备方法 |
-
2010
- 2010-02-19 US US12/708,539 patent/US9150894B2/en active Active
- 2010-02-19 PT PT107076945T patent/PT2398817E/pt unknown
- 2010-02-19 CA CA2748932A patent/CA2748932C/en active Active
- 2010-02-19 CN CN201080008292.3A patent/CN102325785B/zh active Active
- 2010-02-19 MX MX2011008621A patent/MX2011008621A/es active IP Right Grant
- 2010-02-19 SI SI201030966T patent/SI2398817T2/sl unknown
- 2010-02-19 BR BRPI1008300-6A patent/BRPI1008300B1/pt active IP Right Grant
- 2010-02-19 UA UAA201111075A patent/UA105785C2/uk unknown
- 2010-02-19 PL PL10707694T patent/PL2398817T5/pl unknown
- 2010-02-19 RU RU2011137010/04A patent/RU2526391C2/ru active
- 2010-02-19 HU HUE10707694A patent/HUE026818T2/en unknown
- 2010-02-19 JP JP2011551028A patent/JP5752609B2/ja active Active
- 2010-02-19 WO PCT/NO2010/000066 patent/WO2010095953A1/en active Application Filing
- 2010-02-19 DK DK10707694.5T patent/DK2398817T4/da active
- 2010-02-19 KR KR1020117021714A patent/KR101666144B1/ko active IP Right Grant
- 2010-02-19 ES ES10707694T patent/ES2543457T5/es active Active
- 2010-02-19 EP EP10707694.5A patent/EP2398817B2/en active Active
- 2010-02-19 AU AU2010216475A patent/AU2010216475B2/en active Active
-
2011
- 2011-06-30 ZA ZA2011/04848A patent/ZA201104848B/en unknown
- 2011-08-01 IL IL214374A patent/IL214374A/en active IP Right Grant
-
2015
- 2015-07-10 HR HRP20150761TT patent/HRP20150761T4/hr unknown
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