DK21184A - Fremgangsmaade til fremstilling af human vaevsplasminogenaktivator (tpa) i vaertsceller, udtrykkelsesvektor for tpa og et dhfr-protein, celler transformeret med vektoren, og fremgangsmaade til foroegelse af vaertscellernes produktion af tpa - Google Patents

Fremgangsmaade til fremstilling af human vaevsplasminogenaktivator (tpa) i vaertsceller, udtrykkelsesvektor for tpa og et dhfr-protein, celler transformeret med vektoren, og fremgangsmaade til foroegelse af vaertscellernes produktion af tpa Download PDF

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Publication number
DK21184A
DK21184A DK21184A DK21184A DK21184A DK 21184 A DK21184 A DK 21184A DK 21184 A DK21184 A DK 21184A DK 21184 A DK21184 A DK 21184A DK 21184 A DK21184 A DK 21184A
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DK
Denmark
Prior art keywords
procedure
tpa
vector
host cells
plasminogen activator
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DK21184A
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English (en)
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DK21184D0 (da
DK174081B1 (da
Inventor
Arthur David Levinson
Christian Clinton Simonsen
Elizabeth Mcleod Yelverton
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Genentech Inc
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Publication of DK21184A publication Critical patent/DK21184A/da
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Publication of DK174081B1 publication Critical patent/DK174081B1/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Magnetic Resonance Imaging Apparatus (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Electrotherapy Devices (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
DK198400211A 1983-01-19 1984-01-18 Fremgangsmåde til fremstilling af human vævsplasminogenaktivator (tPA) i værtsceller, ekspressionsvektorer for tPA og et DHFR-protein, rekombinante celler transformeret med vektoren, fremgangsmåde til amplifikation af DNA sekvensen,...... DK174081B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45915383A 1983-01-19 1983-01-19
US45915383 1983-01-19

Publications (3)

Publication Number Publication Date
DK21184D0 DK21184D0 (da) 1984-01-18
DK21184A true DK21184A (da) 1984-08-24
DK174081B1 DK174081B1 (da) 2002-05-27

Family

ID=23823628

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198400211A DK174081B1 (da) 1983-01-19 1984-01-18 Fremgangsmåde til fremstilling af human vævsplasminogenaktivator (tPA) i værtsceller, ekspressionsvektorer for tPA og et DHFR-protein, rekombinante celler transformeret med vektoren, fremgangsmåde til amplifikation af DNA sekvensen,......

Country Status (12)

Country Link
EP (1) EP0117059B1 (da)
JP (1) JPS59183693A (da)
KR (1) KR840007255A (da)
AT (1) ATE59410T1 (da)
AU (1) AU572108B2 (da)
CA (1) CA1271149A (da)
DE (1) DE3483839D1 (da)
DK (1) DK174081B1 (da)
ES (1) ES529023A0 (da)
IE (1) IE56716B1 (da)
MY (1) MY101958A (da)
ZA (1) ZA84375B (da)

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US4703008A (en) * 1983-12-13 1987-10-27 Kiren-Amgen, Inc. DNA sequences encoding erythropoietin
NZ210501A (en) * 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
GB8415186D0 (en) * 1984-06-14 1984-07-18 Ciba Geigy Ag Polypeptides
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FI89723C (fi) * 1984-10-01 1993-11-10 Genzyme Corp Foerfarande foer framstaellning av vaevnadsplasminogenaktivator, kodande rekombinant-dna daerav och en transformanscell
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US5672502A (en) * 1985-06-28 1997-09-30 Celltech Therapeutics Limited Animal cell culture
AU593264B2 (en) * 1985-07-10 1990-02-08 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Chromosomal DNA sequence, expression vector for human tissue plasminogen activating factor, cultured cells transfected with same and method of producing said activating factor
NZ217331A (en) * 1985-08-26 1989-05-29 Lilly Co Eli Tissue plasminogen activator derivatives and genetically engineered product
GB8528321D0 (en) * 1985-11-18 1985-12-24 Ciba Geigy Ag Modified fibrinolytic agents
JPH0714341B2 (ja) * 1985-11-20 1995-02-22 鐘淵化学工業株式会社 蛋白質の高生産株の取得方法
US4851517A (en) * 1986-02-26 1989-07-25 Monsanto Company Tissue plasminogen activator oligosaccharide from normal human colon cells
US4751084A (en) * 1986-02-26 1988-06-14 Monsanto Company Tissue plasminogen activator from normal human colon cells
US4927630A (en) * 1986-02-26 1990-05-22 Monsanto Company Tissue plasminogen activator from normal human colon cells
US5132214A (en) * 1986-04-09 1992-07-21 Monsanto Company Large scale production of plasminogen activator from normal human colon cells
CA1297434C (en) * 1986-04-14 1992-03-17 Kenji Murakami Method of producing peptides, recombinant plasmid for use in the same and animal cells transformed with the same
JP2585532B2 (ja) * 1986-05-10 1997-02-26 三井東圧化学株式会社 動物細胞の形質転換体及びそれを得る方法並びにその形質転換体を用いてｔ−ＰＡを生産する方法
JPS62265992A (ja) * 1986-05-12 1987-11-18 Ajinomoto Co Inc ヒト細胞を用いる有用物質の製造方法
IL82746A (en) * 1986-06-06 1994-10-07 Genentech Inc A process for producing a biologically active tissue plasminogen activator
JPS63105675A (ja) * 1986-10-23 1988-05-10 Green Cross Corp:The ヒトウロキナ−ゼの製造方法
PT86162B (pt) * 1986-11-21 1990-11-20 Smithkline Beecham Corp Expressao de proteina recombinante
ZA879286B (en) 1986-12-16 1988-10-26 Smith Kline Rit New plasminogen activators
EP0281246A3 (en) * 1987-02-04 1989-07-12 Invitron Corporation Method to enhance amplification and expression of foreign genes
NZ219516A (en) * 1987-02-10 1991-02-26 Wellcome Found Fusion proteins of hbcag (hepatitis b virus core antigen)
US5013653A (en) * 1987-03-20 1991-05-07 Creative Biomolecules, Inc. Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
US5215896A (en) * 1987-03-20 1993-06-01 Creative Biomolecules, Inc. Leader sequences for the production of recombinant proteins
CA1341345C (en) * 1987-05-08 2002-03-05 Hanne Ranch Johansen Expression of foreign genes in drosophila cells
US5681713A (en) * 1987-05-08 1997-10-28 Smithkline Beecham Corporation Expression of heterologous proteins in Drosophila cells
US4873316A (en) * 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
US5017478A (en) * 1987-07-16 1991-05-21 Berlex Laboratories, Inc. Transfected cells containing plasmids having genes oriented in opposing directions and methods of using
EP0322049B1 (en) * 1987-12-21 1993-04-07 Unilever N.V. Nickel/silica catalysts and their preparation
JP3456992B2 (ja) * 1988-12-08 2003-10-14 デイモン・バイオテック・インコーポレーテッド 変異型ｄｈｆｒ遺伝子を使用した発現誘導方法
US5733761A (en) * 1991-11-05 1998-03-31 Transkaryotic Therapies, Inc. Protein production and protein delivery
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
TW402639B (en) * 1992-12-03 2000-08-21 Transkaryotic Therapies Inc Protein production and protein delivery
JP5648912B2 (ja) 2008-02-27 2015-01-07 国立大学法人北海道大学 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用
JP5704753B2 (ja) 2008-12-22 2015-04-22 国立大学法人北海道大学 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用
RU2639288C2 (ru) 2009-08-11 2017-12-20 Дженентек, Инк. Получение белков в культуральных средах без глутамина
RU2500817C1 (ru) * 2012-07-19 2013-12-10 Общество с ограниченной ответственностью "Международный биотехнологический центр "Генериум" ("МБЦ "Генериум") РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК рВК415, КОДИРУЮЩАЯ ПОЛИПЕПТИД РЕКОМБИНАНТНОГО ТКАНЕВОГО АКТИВАТОРА ПЛАЗМИНОГЕНА ЧЕЛОВЕКА, ЛИНИЯ КЛЕТОК Cricetulus griseus CHO 1F8 - ПРОДУЦЕНТ РЕКОМБИНАНТНОГО ТКАНЕВОГО АКТИВАТОРА ПЛАЗМИНОГЕНА ЧЕЛОВЕКА И СПОСОБ ПОЛУЧЕНИЯ И ВЫДЕЛЕНИЯ ПОЛИПЕПТИДА, ОБЛАДАЮЩЕГО АКТИВНОСТЬЮ ТКАНЕВОГО АКТИВАТОРА ПЛАЗМИНОГЕНА
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Also Published As

Publication number Publication date
ES8507610A1 (es) 1985-10-01
CA1271149A (en) 1990-07-03
EP0117059A3 (en) 1986-03-05
JPS59183693A (ja) 1984-10-18
DE3483839D1 (de) 1991-02-07
AU572108B2 (en) 1988-05-05
IE840097L (en) 1984-07-19
EP0117059A2 (en) 1984-08-29
DK21184D0 (da) 1984-01-18
ATE59410T1 (de) 1991-01-15
MY101958A (en) 1992-02-15
ZA84375B (en) 1985-02-27
IE56716B1 (en) 1991-11-20
DK174081B1 (da) 2002-05-27
KR840007255A (ko) 1984-12-06
ES529023A0 (es) 1985-10-01
AU2353484A (en) 1984-07-26
EP0117059B1 (en) 1990-12-27

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