DK2046369T3 - Rekombinant vært til fremstilling af l-asparaginase ii - Google Patents
Rekombinant vært til fremstilling af l-asparaginase ii Download PDFInfo
- Publication number
- DK2046369T3 DK2046369T3 DK07840229.4T DK07840229T DK2046369T3 DK 2046369 T3 DK2046369 T3 DK 2046369T3 DK 07840229 T DK07840229 T DK 07840229T DK 2046369 T3 DK2046369 T3 DK 2046369T3
- Authority
- DK
- Denmark
- Prior art keywords
- asparaginase
- recombinant
- enzyme
- seq
- host cell
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
- C12N9/82—Asparaginase (3.5.1.1)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Claims (15)
1. Rekombinant Escherichia co/z-værtscelle til fremstilling af et Escherichia coli L-asparaginase Il-enzym, der omfatter et Escherichia co/z'-kromosom og mindst én kopi af en rekombinant ekstrakromosomal vektor, hvor den rekombinante ekstrakromosomale vektor koder for en underenhed af L-asparaginase Il-enzymet, hvor værtscellekromosomet ligeledes koder for den samme underenhed af L-asparaginase Il-enzymet, hvor værtkromosomet ikke koder for nogen anden isoform af L-asparaginase II, hvor den kodede L-asparaginase II-underenhed omfatter SEQ ID NO: 1; og den rekombinante ekstrakromosomale vektor omfatter et DNA-molekyle, der omfatter SEQ ID NO: 2.
2. Rekombinant Escherichia co/z-værtscelle ifølge krav 1, hvor den ekstrakromosomale vektor er et plasmid.
3. Rekombinant Escherichia co/z-værtscelle ifølge krav 1, hvor den rekombinante ekstrakromosomale vektor omfatter et DNA-molekyle, der koder for underenheden af L-Asparaginase Il-enzymet, der er operativt bundet til en egnet promotor.
4. Rekombinant Escherichia cø/z-værtscelle ifølge krav 3, hvor promotoren er udvalgt fra gruppen bestående af T7, araB, λΡκ/λΡΓ , phoA, tre og trp-promotorer.
5. Rekombinant Escherichia co/z-værtscelle ifølge krav 3, hvor den rekombinante ekstrakromosomale vektor endvidere omfatter en operatør, et ribosombindingssted, en signalsekvens, en transskriptionsterminator, en antibiotisk selektionsmarkør, et replikationsorigin og en reguleret kopi af repressoren.
6. Rekombinant Escherichia co/z-værtscelle ifølge krav 3, hvor kromosomet omfatter et DNA-molekyle ifølge SEQ ID NO: 3.
7. Isoleret nukleinsyremolekyle, der koder for en L-asparaginase II-enzymunderenhed af SEQ ID NO: 1, hvor det isolerede nukleinsyremolekyle består af en nukleinsyre udvalgt fra gruppen bestående af SEQ ID NO: 2 og SEQ ID NO: 3.
8. Rekombinant ekstrakromosomal vektor, hvor den rekombinante ekstrakromosomale vektor omfatter SEQ ID NO: 2 og koder for en underenhed af L-asparaginase Il-enzymet.
9. Ekstrakromosomal vektor ifølge krav 8, hvor vektoren er et plasmid.
10. Ekstrakromosomal vektor ifølge krav 9, hvor vektoren er plasmid pEN537.
11. Escherichia co/z'-værtscelle, der omfatter plasmidet ifølge krav 2, hvor plasmidet er pEN537, betegnet som EN538 og deponeret under ATCC-nr. PTA 7490.
12. Fremgangsmåde til fremstilling af en rekombinant L-asparaginase Il-enzym i alt væsentligt fri for andre L-asparaginase II-isomerer, hvilken fremgangsmåde omfatter dyrkning af værtscellen ifølge krav 11 og isolering af det fremstillede L-asparaginase Il-enzym.
13. Isoleret DNA-molekyle, der koder for en underenhed af L-asparaginase Il-enzym, der omfatter SEQ ID NO: 2.
14. Rekombinant ekstrakromosomal vektor ifølge krav 8, hvor SEQ ID NO: 2 er operativt bundet til en egnet promotor.
15. Rekombinant ekstrakromosomal vektor ifølge krav 14, hvor promotoren er udvalgt fra gruppen bestående af T7, araB, λΡ^/λΡ^ phoA, tre og trp-promotorer.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US81781706P | 2006-06-30 | 2006-06-30 | |
| PCT/US2007/070706 WO2008011234A2 (en) | 2006-06-30 | 2007-06-08 | Recombinant host for producing l-asparaginase ii |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2046369T3 true DK2046369T3 (da) | 2015-11-30 |
Family
ID=38957454
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK07840229.4T DK2046369T3 (da) | 2006-06-30 | 2007-06-08 | Rekombinant vært til fremstilling af l-asparaginase ii |
Country Status (17)
| Country | Link |
|---|---|
| US (2) | US7807436B2 (da) |
| EP (1) | EP2046369B1 (da) |
| JP (1) | JP5308333B2 (da) |
| KR (1) | KR101408062B1 (da) |
| CN (1) | CN101484181B (da) |
| AU (1) | AU2007275535B2 (da) |
| CA (1) | CA2652718A1 (da) |
| DK (1) | DK2046369T3 (da) |
| ES (1) | ES2553329T3 (da) |
| HU (1) | HUE025738T2 (da) |
| IL (1) | IL195168A (da) |
| MX (1) | MX2009000284A (da) |
| NZ (1) | NZ572519A (da) |
| PL (1) | PL2046369T3 (da) |
| PT (1) | PT2046369E (da) |
| TW (1) | TWI427148B (da) |
| WO (1) | WO2008011234A2 (da) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR102014000585B1 (pt) | 2014-01-10 | 2021-01-12 | Coppe/Ufrj-Instituto Alberto Luiz Coimbra De Pós-Graduação | l-asparaginase recombinante de zymomonas |
| CN105802948B (zh) | 2014-12-29 | 2020-06-09 | 江苏众红生物工程创药研究院有限公司 | 聚乙二醇定点修饰的门冬酰胺酶及其制备方法与应用 |
| US10480014B2 (en) * | 2015-06-15 | 2019-11-19 | Ajou University Industry-Academic Cooperation Foundation | Method for producing natural rubber by using recombinant microorganism |
| KR101837636B1 (ko) * | 2016-06-14 | 2018-03-12 | 전남대학교산학협력단 | 코리네박테리움 재조합 균주를 이용한 아스파라기나제의 분비생산 방법 |
| CN108795832B (zh) * | 2017-04-26 | 2021-05-14 | 江苏恒瑞医药股份有限公司 | 一种内源l-门冬酰胺酶ii基因敲除的宿主菌、其制备方法及其应用 |
| JP7358347B2 (ja) * | 2017-10-27 | 2023-10-10 | フェネックス インク． | 組み換えＥ．ｃｏｌｉアスパラギナーゼの製造のための方法 |
| AU2018354067B2 (en) | 2017-10-27 | 2023-03-30 | Pfenex Inc. | Method for production of recombinant Erwinia asparaginase |
| WO2020123824A1 (en) | 2018-12-13 | 2020-06-18 | Huya Bioscience International, Llc | Sulcardine administration for treatment of acute atrial fibrillation |
| KR20230125179A (ko) | 2020-12-23 | 2023-08-29 | 재즈 파마슈티칼즈 아일랜드 리미티드 | 전하-차폐 융합 단백질의 정제 방법 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5324844A (en) | 1989-04-19 | 1994-06-28 | Enzon, Inc. | Active carbonates of polyalkylene oxides for modification of polypeptides |
| US5122614A (en) | 1989-04-19 | 1992-06-16 | Enzon, Inc. | Active carbonates of polyalkylene oxides for modification of polypeptides |
| US5643575A (en) | 1993-10-27 | 1997-07-01 | Enzon, Inc. | Non-antigenic branched polymer conjugates |
| US5919455A (en) | 1993-10-27 | 1999-07-06 | Enzon, Inc. | Non-antigenic branched polymer conjugates |
| US5615460A (en) * | 1994-06-06 | 1997-04-01 | The Procter & Gamble Company | Female component for refastenable fastening device having regions of differential extensibility |
| US6180095B1 (en) | 1997-12-17 | 2001-01-30 | Enzon, Inc. | Polymeric prodrugs of amino- and hydroxyl-containing bioactive agents |
| US5965119A (en) | 1997-12-30 | 1999-10-12 | Enzon, Inc. | Trialkyl-lock-facilitated polymeric prodrugs of amino-containing bioactive agents |
| US6958212B1 (en) | 1999-02-01 | 2005-10-25 | Eidgenossische Technische Hochschule Zurich | Conjugate addition reactions for the controlled delivery of pharmaceutically active compounds |
| US7122189B2 (en) | 2002-08-13 | 2006-10-17 | Enzon, Inc. | Releasable polymeric conjugates based on aliphatic biodegradable linkers |
| US7087229B2 (en) | 2003-05-30 | 2006-08-08 | Enzon Pharmaceuticals, Inc. | Releasable polymeric conjugates based on aliphatic biodegradable linkers |
| CN100502944C (zh) * | 2005-09-16 | 2009-06-24 | 北京双鹭药业股份有限公司 | 一种天门冬酰胺酶注射液、其制备方法及应用 |
-
2007
- 2007-06-08 EP EP07840229.4A patent/EP2046369B1/en active Active
- 2007-06-08 CN CN2007800248607A patent/CN101484181B/zh active Active
- 2007-06-08 CA CA002652718A patent/CA2652718A1/en not_active Abandoned
- 2007-06-08 PT PT78402294T patent/PT2046369E/pt unknown
- 2007-06-08 JP JP2009518433A patent/JP5308333B2/ja active Active
- 2007-06-08 PL PL07840229T patent/PL2046369T3/pl unknown
- 2007-06-08 DK DK07840229.4T patent/DK2046369T3/da active
- 2007-06-08 US US11/759,988 patent/US7807436B2/en active Active
- 2007-06-08 AU AU2007275535A patent/AU2007275535B2/en active Active
- 2007-06-08 WO PCT/US2007/070706 patent/WO2008011234A2/en active Application Filing
- 2007-06-08 ES ES07840229.4T patent/ES2553329T3/es active Active
- 2007-06-08 KR KR1020087028792A patent/KR101408062B1/ko active IP Right Grant
- 2007-06-08 HU HUE07840229A patent/HUE025738T2/en unknown
- 2007-06-08 MX MX2009000284A patent/MX2009000284A/es active IP Right Grant
- 2007-06-08 NZ NZ572519A patent/NZ572519A/en unknown
- 2007-06-26 TW TW096123026A patent/TWI427148B/zh not_active IP Right Cessation
-
2008
- 2008-11-09 IL IL195168A patent/IL195168A/en active IP Right Grant
-
2010
- 2010-05-04 US US12/773,320 patent/US20100273236A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| KR20090033175A (ko) | 2009-04-01 |
| EP2046369A2 (en) | 2009-04-15 |
| ES2553329T3 (es) | 2015-12-07 |
| AU2007275535A1 (en) | 2008-01-24 |
| IL195168A (en) | 2014-12-31 |
| TWI427148B (zh) | 2014-02-21 |
| WO2008011234A3 (en) | 2008-10-16 |
| IL195168A0 (en) | 2011-08-01 |
| NZ572519A (en) | 2011-06-30 |
| WO2008011234A2 (en) | 2008-01-24 |
| EP2046369B1 (en) | 2015-08-12 |
| PT2046369E (pt) | 2015-11-26 |
| TW200817518A (en) | 2008-04-16 |
| CN101484181B (zh) | 2012-12-26 |
| US20100273236A1 (en) | 2010-10-28 |
| PL2046369T3 (pl) | 2016-01-29 |
| AU2007275535B2 (en) | 2012-08-16 |
| CA2652718A1 (en) | 2008-01-24 |
| JP2009542219A (ja) | 2009-12-03 |
| JP5308333B2 (ja) | 2013-10-09 |
| MX2009000284A (es) | 2009-01-26 |
| HUE025738T2 (en) | 2016-04-28 |
| KR101408062B1 (ko) | 2014-06-20 |
| CN101484181A (zh) | 2009-07-15 |
| US7807436B2 (en) | 2010-10-05 |
| US20080063632A1 (en) | 2008-03-13 |
| EP2046369A4 (en) | 2010-04-21 |
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