ES2553329T3 - Huésped recombinante para la producción de L-asparaginasa II - Google Patents

Huésped recombinante para la producción de L-asparaginasa II Download PDF

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Publication number
ES2553329T3
ES2553329T3 ES07840229.4T ES07840229T ES2553329T3 ES 2553329 T3 ES2553329 T3 ES 2553329T3 ES 07840229 T ES07840229 T ES 07840229T ES 2553329 T3 ES2553329 T3 ES 2553329T3
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Spain
Prior art keywords
asparaginase
recombinant
enzyme
escherichia coli
subunit
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES07840229.4T
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English (en)
Inventor
David R. Filpula
Maoliang Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sigma-Tau Pharm Ltd
Servier IP UK Ltd
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Sigma-Tau Pharm Ltd
Sigma Tau Pharma Ltd
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Publication of ES2553329T3 publication Critical patent/ES2553329T3/es
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/82—Asparaginase (3.5.1.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

Una célula huésped de Escherichia coli recombinante para producir una enzima L-asparaginasa II de Escherichia coli, que comprende un cromosoma de Escherichia coli y al menos una copia de un vector extracromosómico recombinante, en donde el vector extracromosómico recombinante codifica una subunidad de la enzima Lasparaginasa II, en donde el cromosoma de la célula huésped también codifica la misma subunidad de la enzima Lasparaginasa II, en donde el cromosoma huésped no codifica ninguna otra isoforma de L-asparaginasa II, en donde la subunidad de L-asparaginasa II codificada comprende SEQ ID NO: 1; y el vector extracromosómico recombinante comprende una molécula de ADN que comprende SEQ ID NO: 2.

Description

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Claims (1)

  1. imagen1
ES07840229.4T 2006-06-30 2007-06-08 Huésped recombinante para la producción de L-asparaginasa II Active ES2553329T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US81781706P 2006-06-30 2006-06-30
US817817P 2006-06-30
PCT/US2007/070706 WO2008011234A2 (en) 2006-06-30 2007-06-08 Recombinant host for producing l-asparaginase ii

Publications (1)

Publication Number Publication Date
ES2553329T3 true ES2553329T3 (es) 2015-12-07

Family

ID=38957454

Family Applications (1)

Application Number Title Priority Date Filing Date
ES07840229.4T Active ES2553329T3 (es) 2006-06-30 2007-06-08 Huésped recombinante para la producción de L-asparaginasa II

Country Status (17)

Country Link
US (2) US7807436B2 (es)
EP (1) EP2046369B1 (es)
JP (1) JP5308333B2 (es)
KR (1) KR101408062B1 (es)
CN (1) CN101484181B (es)
AU (1) AU2007275535B2 (es)
CA (1) CA2652718A1 (es)
DK (1) DK2046369T3 (es)
ES (1) ES2553329T3 (es)
HU (1) HUE025738T2 (es)
IL (1) IL195168A (es)
MX (1) MX2009000284A (es)
NZ (1) NZ572519A (es)
PL (1) PL2046369T3 (es)
PT (1) PT2046369E (es)
TW (1) TWI427148B (es)
WO (1) WO2008011234A2 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR102014000585B1 (pt) 2014-01-10 2021-01-12 Coppe/Ufrj-Instituto Alberto Luiz Coimbra De Pós-Graduação l-asparaginase recombinante de zymomonas
CN105802948B (zh) 2014-12-29 2020-06-09 江苏众红生物工程创药研究院有限公司 聚乙二醇定点修饰的门冬酰胺酶及其制备方法与应用
US10480014B2 (en) * 2015-06-15 2019-11-19 Ajou University Industry-Academic Cooperation Foundation Method for producing natural rubber by using recombinant microorganism
KR101837636B1 (ko) * 2016-06-14 2018-03-12 전남대학교산학협력단 코리네박테리움 재조합 균주를 이용한 아스파라기나제의 분비생산 방법
CN108795832B (zh) * 2017-04-26 2021-05-14 江苏恒瑞医药股份有限公司 一种内源l-门冬酰胺酶ii基因敲除的宿主菌、其制备方法及其应用
JP7358347B2 (ja) * 2017-10-27 2023-10-10 フェネックス インク． 組み換えＥ．ｃｏｌｉアスパラギナーゼの製造のための方法
AU2018354067B2 (en) 2017-10-27 2023-03-30 Pfenex Inc. Method for production of recombinant Erwinia asparaginase
WO2020123824A1 (en) 2018-12-13 2020-06-18 Huya Bioscience International, Llc Sulcardine administration for treatment of acute atrial fibrillation
KR20230125179A (ko) 2020-12-23 2023-08-29 재즈 파마슈티칼즈 아일랜드 리미티드 전하-차폐 융합 단백질의 정제 방법

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5324844A (en) 1989-04-19 1994-06-28 Enzon, Inc. Active carbonates of polyalkylene oxides for modification of polypeptides
US5122614A (en) 1989-04-19 1992-06-16 Enzon, Inc. Active carbonates of polyalkylene oxides for modification of polypeptides
US5643575A (en) 1993-10-27 1997-07-01 Enzon, Inc. Non-antigenic branched polymer conjugates
US5919455A (en) 1993-10-27 1999-07-06 Enzon, Inc. Non-antigenic branched polymer conjugates
US5615460A (en) * 1994-06-06 1997-04-01 The Procter & Gamble Company Female component for refastenable fastening device having regions of differential extensibility
US6180095B1 (en) 1997-12-17 2001-01-30 Enzon, Inc. Polymeric prodrugs of amino- and hydroxyl-containing bioactive agents
US5965119A (en) 1997-12-30 1999-10-12 Enzon, Inc. Trialkyl-lock-facilitated polymeric prodrugs of amino-containing bioactive agents
US6958212B1 (en) 1999-02-01 2005-10-25 Eidgenossische Technische Hochschule Zurich Conjugate addition reactions for the controlled delivery of pharmaceutically active compounds
US7122189B2 (en) 2002-08-13 2006-10-17 Enzon, Inc. Releasable polymeric conjugates based on aliphatic biodegradable linkers
US7087229B2 (en) 2003-05-30 2006-08-08 Enzon Pharmaceuticals, Inc. Releasable polymeric conjugates based on aliphatic biodegradable linkers
CN100502944C (zh) * 2005-09-16 2009-06-24 北京双鹭药业股份有限公司 一种天门冬酰胺酶注射液、其制备方法及应用

Also Published As

Publication number Publication date
KR20090033175A (ko) 2009-04-01
EP2046369A2 (en) 2009-04-15
AU2007275535A1 (en) 2008-01-24
IL195168A (en) 2014-12-31
TWI427148B (zh) 2014-02-21
WO2008011234A3 (en) 2008-10-16
IL195168A0 (en) 2011-08-01
NZ572519A (en) 2011-06-30
WO2008011234A2 (en) 2008-01-24
EP2046369B1 (en) 2015-08-12
PT2046369E (pt) 2015-11-26
TW200817518A (en) 2008-04-16
CN101484181B (zh) 2012-12-26
US20100273236A1 (en) 2010-10-28
PL2046369T3 (pl) 2016-01-29
AU2007275535B2 (en) 2012-08-16
DK2046369T3 (en) 2015-11-30
CA2652718A1 (en) 2008-01-24
JP2009542219A (ja) 2009-12-03
JP5308333B2 (ja) 2013-10-09
MX2009000284A (es) 2009-01-26
HUE025738T2 (en) 2016-04-28
KR101408062B1 (ko) 2014-06-20
CN101484181A (zh) 2009-07-15
US7807436B2 (en) 2010-10-05
US20080063632A1 (en) 2008-03-13
EP2046369A4 (en) 2010-04-21

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