DK2027272T3 - Plasmid DNA PREPARATIONS AND METHODS FOR THE MANUFACTURE OF SAME - Google Patents
Plasmid DNA PREPARATIONS AND METHODS FOR THE MANUFACTURE OF SAME Download PDFInfo
- Publication number
- DK2027272T3 DK2027272T3 DK07784120.3T DK07784120T DK2027272T3 DK 2027272 T3 DK2027272 T3 DK 2027272T3 DK 07784120 T DK07784120 T DK 07784120T DK 2027272 T3 DK2027272 T3 DK 2027272T3
- Authority
- DK
- Denmark
- Prior art keywords
- plasmid dna
- dna
- endotoxin
- plasmid
- genes
- Prior art date
Links
- 239000013612 plasmid Substances 0.000 title claims description 69
- 238000000034 method Methods 0.000 title claims description 22
- 238000004519 manufacturing process Methods 0.000 title claims description 14
- 238000002360 preparation method Methods 0.000 title claims description 9
- 239000002158 endotoxin Substances 0.000 claims description 53
- 108090000623 proteins and genes Proteins 0.000 claims description 41
- 241000894006 Bacteria Species 0.000 claims description 30
- 238000012217 deletion Methods 0.000 claims description 24
- 230000037430 deletion Effects 0.000 claims description 24
- 241000588724 Escherichia coli Species 0.000 claims description 10
- 101100021843 Shigella flexneri lpxM1 gene Proteins 0.000 claims description 9
- 101100021844 Shigella flexneri lpxM2 gene Proteins 0.000 claims description 9
- 101150060640 lpxM gene Proteins 0.000 claims description 9
- 239000006166 lysate Substances 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 9
- 238000000746 purification Methods 0.000 claims description 8
- 239000006151 minimal media Substances 0.000 claims description 5
- 101000980463 Treponema pallidum (strain Nichols) Chaperonin GroEL Proteins 0.000 claims description 4
- 230000002934 lysing effect Effects 0.000 claims description 3
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- 108020004414 DNA Proteins 0.000 description 77
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- 101100348617 Candida albicans (strain SC5314 / ATCC MYA-2876) NIK1 gene Proteins 0.000 description 4
- 101100007329 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) COS1 gene Proteins 0.000 description 4
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- 108700039887 Essential Genes Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229930186217 Glycolipid Natural products 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
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- 241000239220 Limulus polyphemus Species 0.000 description 1
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- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 108091061939 Selfish DNA Proteins 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 108700012920 TNF Proteins 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 229940126575 aminoglycoside Drugs 0.000 description 1
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- 229940041514 candida albicans extract Drugs 0.000 description 1
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- 239000013611 chromosomal DNA Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
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- 125000004122 cyclic group Chemical group 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
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- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 229940125532 enzyme inhibitor Drugs 0.000 description 1
- 239000002532 enzyme inhibitor Substances 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 108091008053 gene clusters Proteins 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000002198 insoluble material Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
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- 210000003292 kidney cell Anatomy 0.000 description 1
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- 231100000518 lethal Toxicity 0.000 description 1
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- GZQKNULLWNGMCW-PWQABINMSA-N lipid A (E. coli) Chemical compound O1[C@H](CO)[C@@H](OP(O)(O)=O)[C@H](OC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCCCC)[C@@H](NC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCC)[C@@H]1OC[C@@H]1[C@@H](O)[C@H](OC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](NC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](OP(O)(O)=O)O1 GZQKNULLWNGMCW-PWQABINMSA-N 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 238000000464 low-speed centrifugation Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 125000001419 myristoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 229940124606 potential therapeutic agent Drugs 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 101150052269 rfaJ gene Proteins 0.000 description 1
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- 239000012137 tryptone Substances 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US80850006P | 2006-05-24 | 2006-05-24 | |
| PCT/US2007/069685 WO2007140274A1 (en) | 2006-05-24 | 2007-05-24 | Plasmid dna preparations and methods for producing same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2027272T3 true DK2027272T3 (en) | 2016-03-14 |
Family
ID=38542027
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK07784120.3T DK2027272T3 (en) | 2006-05-24 | 2007-05-24 | Plasmid DNA PREPARATIONS AND METHODS FOR THE MANUFACTURE OF SAME |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US7935505B2 (enExample) |
| EP (1) | EP2027272B1 (enExample) |
| JP (1) | JP5820564B2 (enExample) |
| DK (1) | DK2027272T3 (enExample) |
| WO (1) | WO2007140274A1 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102171341A (zh) * | 2008-04-30 | 2011-08-31 | 格兰达利斯有限公司 | 高纯度质粒dna制备物及其制备方法 |
| US11274292B2 (en) | 2011-03-29 | 2022-03-15 | Phynexus, Inc. | Devices and methods for plasmid purification |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5997881A (en) * | 1995-11-22 | 1999-12-07 | University Of Maryland, Baltimore | Method of making non-pyrogenic lipopolysaccharide or A |
| ES2195112T5 (es) * | 1996-02-06 | 2015-10-15 | Roche Diagnostics Gmbh | Procedimiento para la preparación de ácido nucleico purificado y el uso del mismo |
| US6080849A (en) | 1997-09-10 | 2000-06-27 | Vion Pharmaceuticals, Inc. | Genetically modified tumor-targeted bacteria with reduced virulence |
-
2007
- 2007-05-24 DK DK07784120.3T patent/DK2027272T3/en active
- 2007-05-24 US US12/294,159 patent/US7935505B2/en active Active
- 2007-05-24 JP JP2009512311A patent/JP5820564B2/ja active Active
- 2007-05-24 EP EP07784120.3A patent/EP2027272B1/en active Active
- 2007-05-24 WO PCT/US2007/069685 patent/WO2007140274A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| JP2009538147A (ja) | 2009-11-05 |
| WO2007140274A1 (en) | 2007-12-06 |
| EP2027272A1 (en) | 2009-02-25 |
| US20090068707A1 (en) | 2009-03-12 |
| JP5820564B2 (ja) | 2015-11-24 |
| EP2027272B1 (en) | 2016-01-06 |
| US7935505B2 (en) | 2011-05-03 |
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