DK1995319T3 - Forbedret bacillusværtscelle - Google Patents

Forbedret bacillusværtscelle

Info

Publication number
DK1995319T3
DK1995319T3 DK08159398.0T DK08159398T DK1995319T3 DK 1995319 T3 DK1995319 T3 DK 1995319T3 DK 08159398 T DK08159398 T DK 08159398T DK 1995319 T3 DK1995319 T3 DK 1995319T3
Authority
DK
Denmark
Prior art keywords
host cell
bacillus host
parent
enhanced
mutant
Prior art date
Application number
DK08159398.0T
Other languages
English (en)
Inventor
Jens Toenne Andersen
Steen Troels Joergensen
Michael Dolbjerg Rasmussen
Peter Bjarke Olsen
Ib Groth Clausen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK1995319T3 publication Critical patent/DK1995319T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/10—Bacillus licheniformis
DK08159398.0T 2002-04-10 2003-03-25 Forbedret bacillusværtscelle DK1995319T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200200534 2002-04-10
EP03709679A EP1497429B1 (en) 2002-04-10 2003-03-25 Improved bacillus host cell

Publications (1)

Publication Number Publication Date
DK1995319T3 true DK1995319T3 (da) 2011-12-05

Family

ID=29286036

Family Applications (2)

Application Number Title Priority Date Filing Date
DK03709679T DK1497429T3 (da) 2002-04-10 2003-03-25 Forbedret bacillusværtcelle
DK08159398.0T DK1995319T3 (da) 2002-04-10 2003-03-25 Forbedret bacillusværtscelle

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK03709679T DK1497429T3 (da) 2002-04-10 2003-03-25 Forbedret bacillusværtcelle

Country Status (8)

Country Link
US (3) US7521204B2 (da)
EP (2) EP1497429B1 (da)
CN (1) CN1659283A (da)
AT (2) ATE522614T1 (da)
AU (1) AU2003214034A1 (da)
DE (1) DE60321901D1 (da)
DK (2) DK1497429T3 (da)
WO (1) WO2003093453A2 (da)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA04011011A (es) 2002-05-14 2005-02-14 Novozymes As Variantes de pectato liasa.
JP2007529993A (ja) * 2003-07-02 2007-11-01 シンジェンタ パーティシペーションズ アーゲー グルカナーゼ、それらをコードする核酸並びにそれらを製造及び使用する方法
AU2013202679B2 (en) * 2003-07-02 2016-03-03 Bp Corporation North America Inc. Glucanases, nucleic acids encoding them and methods for making and using them
EP2216640A1 (en) * 2004-01-09 2010-08-11 Novozymes, Inc. Bacillus licheniformis chromosome
EP2325318A1 (en) * 2004-02-13 2011-05-25 Novozymes A/S Protease variants
CN101006174B (zh) * 2004-06-21 2010-05-26 诺维信公司 以相同取向稳定保持的至少两个orf的多拷贝
EP1612267A1 (en) * 2004-07-02 2006-01-04 GBF Gesellschaft für Biotechnologische Forschung mbH Cellulases from rumen
CA2603140A1 (en) * 2005-03-22 2006-09-28 Novozymes A/S Use of bacillus licheniformis polypeptides in animal feed
CN101541968B (zh) * 2006-11-30 2013-11-13 诺维信公司 重组宿主细胞中的脱氧核糖核酸酶表达
BRPI0720678A2 (pt) * 2006-12-22 2014-02-04 Dsm Ip Assets Bv Uso de peptídeos como coccidiostáticos e/ou hitomonastáticos
US20120329090A1 (en) * 2009-12-21 2012-12-27 Novozymes A/S Methods for Producing Heterologous Polypeptides in Thiol-Disulfide Oxidoreductase-Deficient Bacterial Mutant Cells
EP3481949B1 (en) 2016-07-05 2021-06-09 Novozymes A/S Pectate lyase variants and polynucleotides encoding same
CN106467903B (zh) * 2016-09-18 2019-10-08 齐鲁工业大学 一种适用于制浆过程的地衣芽孢杆菌工程菌及其构建方法
CN106893736B (zh) * 2017-01-24 2020-07-07 湖北大学 一种提高地衣芽胞杆菌外源蛋白分泌水平的方法
AU2019217608A1 (en) 2018-02-06 2020-08-06 Novozymes Bioag A/S Methods of protecting a plant from fungal pests
WO2022129166A1 (en) 2020-12-15 2022-06-23 Novozymes A/S Mutated host cells with reduced cell motility
BR112023027009A2 (pt) 2021-06-24 2024-03-12 Basf Se Célula hospedeira modificada de bacillus licheniformis, e, métodos para produzir um composto de interesse e para aumentar a pureza de um composto de interesse
BR112023027016A2 (pt) 2021-06-24 2024-03-12 Basf Se Célula hospedeira modificada de bacillus compreendendo uma proteína reguladora da matriz extracelular a alterada e/ou uma proteína reguladora da matriz extracelular b alterada, método para produzir um composto de interesse, e, proteínas reguladoras das matrizes extracelulares a e b alteradas
WO2023023642A2 (en) * 2021-08-20 2023-02-23 Danisco Us Inc. Methods and compositions for enhanced protein production in bacillus cells
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2003078A1 (en) 1988-11-18 1990-05-18 Alan Sloma Protease deletion
WO1991002803A1 (en) * 1989-08-25 1991-03-07 Henkel Research Corporation A method of suppressing chromosomal gene expression
DK639689D0 (da) 1989-12-18 1989-12-18 Novo Nordisk As Indfoering af dna i celler
US5294542A (en) * 1991-03-19 1994-03-15 Omnigene, Inc. Residual protease-III
IL103750A0 (en) 1991-11-14 1993-04-04 Novo Nordisk As Bacillus promoter
DK153992D0 (da) 1992-12-22 1992-12-22 Novo Nordisk As Metode
GB2279955B (en) 1993-07-15 1998-02-18 Solvay Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof
CN1192108C (zh) 1994-06-03 2005-03-09 诺沃奇梅兹生物技术有限公司 纯化的毁丝霉属漆酶及编码该酶的核酸
JPH10512450A (ja) 1995-01-23 1998-12-02 ノボ ノルディスク アクティーゼルスカブ 転移によるｄｎａの組込み
DK0934406T3 (da) 1996-08-23 2009-01-26 Peter Ruhdal Jensen Syntetiske promotor biblioteker til udvalgte organismer og promotorer fra sådanne biblioteker
GB9708452D0 (en) * 1997-04-26 1997-06-18 Univ Newcastle Improved prokaryotic expression of protein
US6100063A (en) 1998-02-12 2000-08-08 Novo Nordisk A/S Procaryotic cell comprising at least two copies of a gene
AU2610399A (en) 1998-02-12 1999-08-30 Novo Nordisk A/S A prokaryotic cell comprising two copies of a gene transcribed in different directions
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
US7449320B2 (en) 1998-03-04 2008-11-11 Genencor International, Inc. Truncated forms of pullulanase
EP1290207B1 (en) 2000-05-24 2010-09-08 Novozymes A/S Method for increasing gene copy number in a host cell and resulting host cell
AU2001265835A1 (en) 2000-06-23 2002-01-08 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
EP2072526B1 (en) * 2000-10-06 2012-05-30 Novozymes, Inc. Methods for disrupting a gene involved in PGA synthesis in Bacillus licheniformis

Also Published As

Publication number Publication date
EP1497429A2 (en) 2005-01-19
ATE522614T1 (de) 2011-09-15
US20050244922A1 (en) 2005-11-03
EP1995319A1 (en) 2008-11-26
EP1995319B1 (en) 2011-08-31
AU2003214034A8 (en) 2003-11-17
DK1497429T3 (da) 2008-11-03
US20090203074A1 (en) 2009-08-13
AU2003214034A1 (en) 2003-11-17
US7521204B2 (en) 2009-04-21
WO2003093453A2 (en) 2003-11-13
WO2003093453A3 (en) 2004-05-13
EP1497429B1 (en) 2008-07-02
CN1659283A (zh) 2005-08-24
US20090176290A1 (en) 2009-07-09
US8911969B2 (en) 2014-12-16
ATE399869T1 (de) 2008-07-15
DE60321901D1 (de) 2008-08-14

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