ATE399869T1 - Verbesserte bacillus-wirtszelle - Google Patents

Verbesserte bacillus-wirtszelle

Info

Publication number
ATE399869T1
ATE399869T1 AT03709679T AT03709679T ATE399869T1 AT E399869 T1 ATE399869 T1 AT E399869T1 AT 03709679 T AT03709679 T AT 03709679T AT 03709679 T AT03709679 T AT 03709679T AT E399869 T1 ATE399869 T1 AT E399869T1
Authority
AT
Austria
Prior art keywords
host cell
bacillus host
parent
improved bacillus
mutant
Prior art date
Application number
AT03709679T
Other languages
English (en)
Inventor
Jens Andersen
Steen Joergensen
Michael Rasmussen
Peter Olsen
Ib Clausen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE399869T1 publication Critical patent/ATE399869T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/10—Bacillus licheniformis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • External Artificial Organs (AREA)
AT03709679T 2002-04-10 2003-03-25 Verbesserte bacillus-wirtszelle ATE399869T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DKPA200200534 2002-04-10

Publications (1)

Publication Number Publication Date
ATE399869T1 true ATE399869T1 (de) 2008-07-15

Family

ID=29286036

Family Applications (2)

Application Number Title Priority Date Filing Date
AT08159398T ATE522614T1 (de) 2002-04-10 2003-03-25 Mutierte bacillus licheniformis wirtszelle
AT03709679T ATE399869T1 (de) 2002-04-10 2003-03-25 Verbesserte bacillus-wirtszelle

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT08159398T ATE522614T1 (de) 2002-04-10 2003-03-25 Mutierte bacillus licheniformis wirtszelle

Country Status (8)

Country Link
US (3) US7521204B2 (de)
EP (2) EP1995319B1 (de)
CN (1) CN1659283A (de)
AT (2) ATE522614T1 (de)
AU (1) AU2003214034A1 (de)
DE (1) DE60321901D1 (de)
DK (2) DK1497429T3 (de)
WO (1) WO2003093453A2 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100482791C (zh) 2002-05-14 2009-04-29 诺和酶股份有限公司 果胶酸裂解酶变体
BRPI0412279A (pt) * 2003-07-02 2006-09-19 Diversa Corp glucanases, ácidos nucléicos codificando as mesmas e métodos para preparar e aplicar os mesmos
AU2013202671B2 (en) * 2003-07-02 2016-03-03 Bp Corporation North America Inc. Glucanases, nucleic acids encoding them and methods for making and using them
EP2298797A3 (de) * 2004-01-09 2011-05-18 Novozymes Inc. Gesteigerte Bacillus YweA-Expression
WO2005078074A2 (en) 2004-02-13 2005-08-25 Novozymes A/S Protease variants
EP1766002B1 (de) 2004-06-21 2015-11-18 Novozymes A/S Mehrere kopien wenigstens zweier offener leserrahmen stabil erhalten in gleicher orientierung
EP1612267A1 (de) * 2004-07-02 2006-01-04 GBF Gesellschaft für Biotechnologische Forschung mbH Zellulasen aus Rumen
DK1863834T3 (da) * 2005-03-22 2010-11-01 Novozymes As Polypeptider og nukleinsyrer, der koder disse
WO2008065200A1 (en) * 2006-11-30 2008-06-05 Novozymes A/S Dnase expression in recombinant host cells
EP2091554A2 (de) * 2006-12-22 2009-08-26 DSMIP Assets B.V. Verwendung von polypeptiden als coccidiostatikum und/oder histomonostatikum
US20120329090A1 (en) * 2009-12-21 2012-12-27 Novozymes A/S Methods for Producing Heterologous Polypeptides in Thiol-Disulfide Oxidoreductase-Deficient Bacterial Mutant Cells
CA3028535A1 (en) 2016-07-05 2018-01-11 Novozymes A/S Pectate lyase variants and polynucleotides encoding same
CN106467903B (zh) * 2016-09-18 2019-10-08 齐鲁工业大学 一种适用于制浆过程的地衣芽孢杆菌工程菌及其构建方法
CN106893736B (zh) * 2017-01-24 2020-07-07 湖北大学 一种提高地衣芽胞杆菌外源蛋白分泌水平的方法
BR112020015339A2 (pt) * 2018-02-06 2020-12-08 Novozymes Bioag A/S Métodos para aplicação foliar de uma composição, para controlar ou prevenir uma ou mais doenças de plantas e/ou pragas de plantas em uma planta ou parte da planta e/ou induzir resistência a doenças por um patógeno em uma planta ou parte da planta e para controlar ou prevenir danos por patógenos e/ou danos por pragas em um material de propagação de plantas, uma planta, parte de uma planta e/ou órgão da planta
WO2022129166A1 (en) 2020-12-15 2022-06-23 Novozymes A/S Mutated host cells with reduced cell motility
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
MX2023015460A (es) 2021-06-24 2024-01-18 Basf Se Celula huesped de bacillus mejorada con proteina rema/remb alterada.
BR112023027009A2 (pt) 2021-06-24 2024-03-12 Basf Se Célula hospedeira modificada de bacillus licheniformis, e, métodos para produzir um composto de interesse e para aumentar a pureza de um composto de interesse
WO2023023642A2 (en) * 2021-08-20 2023-02-23 Danisco Us Inc. Methods and compositions for enhanced protein production in bacillus cells

Family Cites Families (18)

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Publication number Priority date Publication date Assignee Title
CA2003078A1 (en) 1988-11-18 1990-05-18 Alan Sloma Protease deletion
WO1991002803A1 (en) * 1989-08-25 1991-03-07 Henkel Research Corporation A method of suppressing chromosomal gene expression
DK639689D0 (da) 1989-12-18 1989-12-18 Novo Nordisk As Indfoering af dna i celler
US5294542A (en) * 1991-03-19 1994-03-15 Omnigene, Inc. Residual protease-III
EP0667910B1 (de) 1991-11-14 2003-08-27 Novozymes A/S Ein bacillus-promotor, der von dem alpha-amylase-promotor einer variante des bacillus licheniformis abstammt
DK153992D0 (da) 1992-12-22 1992-12-22 Novo Nordisk As Metode
GB2279955B (en) 1993-07-15 1998-02-18 Solvay Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof
CN1192108C (zh) 1994-06-03 2005-03-09 诺沃奇梅兹生物技术有限公司 纯化的毁丝霉属漆酶及编码该酶的核酸
DE69631118T2 (de) 1995-01-23 2004-07-01 Novozymes A/S Dna-integration durch transposition
CN1243827C (zh) 1996-08-23 2006-03-01 彼得·鲁戴尔·简森 选定生物体的人工启动子文库和得自这种文库的启动子
GB9708452D0 (en) * 1997-04-26 1997-06-18 Univ Newcastle Improved prokaryotic expression of protein
US6100063A (en) 1998-02-12 2000-08-08 Novo Nordisk A/S Procaryotic cell comprising at least two copies of a gene
JP4350894B2 (ja) 1998-02-12 2009-10-21 ノボザイムス アクティーゼルスカブ 異なる方向に転写される２コピーの遺伝子を含有する原核細胞
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
CN1249226C (zh) 1998-03-04 2006-04-05 金克克国际有限公司 支链淀粉酶的修饰形式
WO2001090393A1 (en) 2000-05-24 2001-11-29 Novozymes A/S Method for increasing gene copy number in a host cell and resulting host cell
WO2002000907A1 (en) 2000-06-23 2002-01-03 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
WO2002029113A2 (en) * 2000-10-06 2002-04-11 Novozymes Biotech, Inc. Methods for monitoring multiple gene expression

Also Published As

Publication number Publication date
EP1995319B1 (de) 2011-08-31
US7521204B2 (en) 2009-04-21
AU2003214034A8 (en) 2003-11-17
ATE522614T1 (de) 2011-09-15
DE60321901D1 (de) 2008-08-14
DK1995319T3 (da) 2011-12-05
DK1497429T3 (da) 2008-11-03
US8911969B2 (en) 2014-12-16
US20050244922A1 (en) 2005-11-03
US20090176290A1 (en) 2009-07-09
WO2003093453A2 (en) 2003-11-13
EP1497429A2 (de) 2005-01-19
CN1659283A (zh) 2005-08-24
US20090203074A1 (en) 2009-08-13
EP1995319A1 (de) 2008-11-26
WO2003093453A3 (en) 2004-05-13
AU2003214034A1 (en) 2003-11-17
EP1497429B1 (de) 2008-07-02

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