DK1941269T3 - Fremgangsmåde til screening af et biologisk mål for svage interaktioner ved anvendelse af svag affinitetskromatografi - Google Patents

Fremgangsmåde til screening af et biologisk mål for svage interaktioner ved anvendelse af svag affinitetskromatografi Download PDF

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Publication number
DK1941269T3
DK1941269T3 DK06813046.7T DK06813046T DK1941269T3 DK 1941269 T3 DK1941269 T3 DK 1941269T3 DK 06813046 T DK06813046 T DK 06813046T DK 1941269 T3 DK1941269 T3 DK 1941269T3
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Denmark
Prior art keywords
affinity
ligands
ligand
target
interactions
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Application number
DK06813046.7T
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English (en)
Inventor
Sten Ohlson
Siamak Shoravi
Tomas Fex
Roland Isaksson
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Transientic Interactions Ab
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Publication of DK1941269T3 publication Critical patent/DK1941269T3/da

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Claims (3)

1. Fremgangsmåde til identifikation af kandidat-lægemiddelmolekyler, som udviser svag affinitet, ved screening af et biologisk mål for transiente svage interaktioner mellem målet og et bibliotek med ligander, hvilken fremgangsmåde omfatter følgende trin: (i) tilvejebringelse af en sammensætning med et biologisk mål; (ii) tilvejebringelse af en flerhed af stationære faser ud fra sammensætningen ved immobilisering af et proteinholdigt mål til en fast bærer i form af funktionaliserede silicapartikler pakket i kromatografibrønde; (iii) transport af en flerhed af ligandsammensætninger til de stationære faser og derved etablering af kontakter mellem liganderne og målene; (iv) indsamling, nedstrøms for de stationære faser, af zonal forsinkelsesinformation for hver ligand og kvantitativ påvisning af liganderne i sammensætninger, der ankommer fra de stationære faser, med henblik på at skelne mellem forskellige ligandaffiniteter for at estimere affiniteten og dynamikken for hver ligand/mål-interaktion; og (v) udvælgelse af ligander, som udviser svag affinitet for målet, hvor liganderne har dissociationskonstanter (Kd) i intervallet fra 0,01 til 10 mM.
2. Fremgangsmåde ifølge krav 1, hvor den zonale forsinkelsesinformation er valgt blandt retentionstid og båndbredde.
3. Fremgangsmåde ifølge krav 1, hvilken fremgangsmåde omfatter kontinuerlig eluering af den stationære fase med en mobil fase.
DK06813046.7T 2005-10-27 2006-10-25 Fremgangsmåde til screening af et biologisk mål for svage interaktioner ved anvendelse af svag affinitetskromatografi DK1941269T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US73080805P 2005-10-27 2005-10-27
PCT/SE2006/050419 WO2007050032A1 (en) 2005-10-27 2006-10-25 A method of screening a biological target for weak interactions using weak affinity chromatography

Publications (1)

Publication Number Publication Date
DK1941269T3 true DK1941269T3 (da) 2016-01-18

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ID=37968063

Family Applications (1)

Application Number Title Priority Date Filing Date
DK06813046.7T DK1941269T3 (da) 2005-10-27 2006-10-25 Fremgangsmåde til screening af et biologisk mål for svage interaktioner ved anvendelse af svag affinitetskromatografi

Country Status (7)

Country Link
US (1) US20080293587A1 (da)
EP (1) EP1941269B8 (da)
DK (1) DK1941269T3 (da)
ES (1) ES2557942T3 (da)
HU (1) HUE030060T2 (da)
PL (1) PL1941269T3 (da)
WO (1) WO2007050032A1 (da)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6123206B2 (ja) * 2011-09-29 2017-05-10 住友ベークライト株式会社 糖鎖精製装置および糖鎖精製方法
US9448243B2 (en) 2013-09-04 2016-09-20 Sten Ohlson Weak affinity chromatography
CN104634909A (zh) * 2013-11-14 2015-05-20 中国科学院上海药物研究所 基于亲和色谱测定超分子相互作用解离速率常数的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE468653B (sv) * 1987-03-06 1993-02-22 Perstorp Biolytica Adsorbent foer isokratisk affinitetskromatografi
SE468238B (sv) * 1990-02-28 1992-11-30 Sten Ohlson Laekemedel med svag affinitet
US6581441B1 (en) * 2002-02-01 2003-06-24 Perseptive Biosystems, Inc. Capillary column chromatography process and system

Also Published As

Publication number Publication date
PL1941269T3 (pl) 2016-04-29
ES2557942T3 (es) 2016-01-29
EP1941269A1 (en) 2008-07-09
US20080293587A1 (en) 2008-11-27
EP1941269B1 (en) 2015-10-21
EP1941269B8 (en) 2015-12-30
WO2007050032A1 (en) 2007-05-03
HUE030060T2 (en) 2017-04-28

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