DK1824961T3 - System til ekspression af melibioseoperon - Google Patents
System til ekspression af melibioseoperon Download PDFInfo
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- DK1824961T3 DK1824961T3 DK05817790.8T DK05817790T DK1824961T3 DK 1824961 T3 DK1824961 T3 DK 1824961T3 DK 05817790 T DK05817790 T DK 05817790T DK 1824961 T3 DK1824961 T3 DK 1824961T3
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Claims (23)
- SYSTEM TIL EKSPRESSION AF MELIBIOSEOPERON1. Vektor, der kan udtrykkes i en vært, og som omfatter melAB-promotoren af melibioseoperonen, der er operabelt forbundet med en transkriptionsenhed, som omfatter en nukleinsyresekvens, der er heterolog med værten, og en signalsekvens, der er operabelt forbundet med nukleinsyresekvensen, og som koder for et signalpeptid, der er valgt fra gruppen bestående af E. coli-signalpeptiderne LamB, MalE, OmpA og PhoA, hvor udtrykkeisen af nukleinsyresekvensen reguleres af melAB-promotoren, og hvor melAB-promotoren mangler! CRP1-bindingsstedet.
- 2. Vektor ifølge krav 1, hvor me/AB-promotoren, der mangler i CRP1 -bindingsstedet, består af sekvensen SEQ ID NO. 1 eller en dermed komplementær sekvens.
- 3. Vektor ifølge krav 1 eller 2, hvor transkriptionsenheden yderligere omfatter en translationsstartregion opstrøms for startpunktet for translationen af transkriptionsenheden, idet translationsstartregionen består af sekvensen AGGAGATATACAT (SEQ ID NO. 2), hvor translationsstartregionen er operabelt forbundet med nukleinsyresekvensen.
- 4. Vektor ifølge et hvilket som helst af kravene 1-3, hvor transkriptionsenheden yderligere omfatter en transkriptionsstopregion, der er rrnB-transkriptionsstopsekvensen.
- 5. Vektor ifølge et hvilket som helst af kravene 1-4, hvor nukleinsyresekvensen koder for et polypeptid.
- 6. Vektor ifølge krav 1-4, hvor nukleinsyresekvensen koder for et antistof.
- 7. Vektor ifølge krav 1-4, hvor nukleinsyresekvensen koder for et Fab-fragment.
- 8. Vektor iføige krav 7, hvor en dicistronisk transkriptionsenhed koder for den tunge og den lette kæde af Fab-fragmentet, mens hver kæde er operabelt forbundet med en signaisekvens og en identisk translationsstartregion opstrøms for startpunktet for translationen af transkriptionsenheden.
- 9. Vektor ifølge et hvilket som helst af kravene 1-8, hvor promotoren og den operabelt forbundne transkriptionsenhed omfatter sekvensen SEQ ID NO. 3 eller en dermed komplementær sekvens.
- 10. Vektor ifølge et hvilket som helst af kravene 1-8, hvor promotoren og den operabelt forbundne transkriptionsenhed omfatter sekvensen SEQ ID NO. 4 eller en dermed komplementær sekvens.
- 11. Vektor ifølge et hvilket som helst af kravene 1-10, hvor vektoren er et autonomt eller selvreplikerende plasmid, et cosmid, en fag, et virus eller et retrovirus.
- 12. Anvendelse af vektoren ifølge et hvilket som helst af kravene 1-11 til reguleret heterolog udtrykkelse af en nukieinsyresekvens i en prokaryot vært.
- 13. Anvendelse af vektoren ifølge krav 12, hvor nukleinsyresekvensen koder for et polypeptid.
- 14. Anvendelse af vektoren ifølge krav 13, hvor polypeptidet er et Fab-fragment, mens de tunge og lette kæder af Fab-fragmentet udtrykkes i ens mængder.
- 15. Isoleret og oprenset nukieinsyresekvens, der kan udtrykkes i en vært, og som omfatter me/AS-promotoren af melibioseoperonen, der er operabelt forbundet med en transkriptionsenhed, som omfatter en nukieinsyresekvens, der er heterolog med værten, og en signalsekvens, der er operabelt forbundet med nukleinsyresekvensen, og som koder for et signalpeptid, der er valgt fra gruppen bestående af E. coli-signalpeptiderne LamB, MalE, OmpA og PhoA, hvor udtrykkeisen af nukleinsyresekvensen reguleres af melAB-promotoren, og hvor me/AS-promotoren mangler i CRP1 -bindingsstedet.
- 16. Isoleret og oprenset nukieinsyresekvens ifølge krav 15, hvor me/AS-promotoren, der mangler i CRP1 -bindingsstedet, består af sekvensen SEQ ID NO. 1 eller en dermed komplementær sekvens.
- 17. Isoleret og oprenset nukieinsyresekvens ifølge krav 15, hvor promotoren og den operabelt forbundne transkriptionsenhed omfatter sekvensen SEQ ID NO. 3 eller en dermed komplementær sekvens.
- 18. Isoleret og oprenset nukieinsyresekvens ifølge krav 15, hvor promotoren og den operabelt forbundne transkriptionsenhed omfatter sekvensen SEQ ID NO. 4 eller en dermed komplementær sekvens.
- 19. Prokaryot vært, der er transformeret med vektoren ifølge et hvilket som helst af kravene 1-11.
- 20. Prokaryot vært, der er transformeret med den isolerede og oprensede nukieinsyresekvens ifølge et hvilket som helst af kravene 15-18.
- 21. Fremgangsmåde til fremstilling af et polypeptid i en vært, hvilken fremgangsmåde omfatter følgende trin: a) konstruktion af en vektor ifølge et hvilket som helst af kravene 5-11, b) transformation af en prokaryot vært med vektoren, c) muliggørelse af udtrykkelse af polypeptidet i et celledyrkningssystem under passende betingelser, d) isolering af polypeptidet ud fra celledyrkningssystemet.
- 22. Fremgangsmåde ifølge krav 21, hvor det fremstillede polypeptid er et Fab-fragment, mens de tunge og lette kæder af Fab-fragmentet udtrykkes i celledyrkningssystemet i ens mængder.
- 23. Fremgangsmåde ifølge krav 21 eller 22, hvor udtrykkeisen af polypeptidet udføres i glycerolholdigt medium.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP04028920 | 2004-12-07 | ||
| PCT/EP2005/013012 WO2006061173A2 (en) | 2004-12-07 | 2005-12-05 | Melibiose operon expression system |
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| Publication Number | Publication Date |
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| DK1824961T3 true DK1824961T3 (da) | 2017-01-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| DK05817790.8T DK1824961T3 (da) | 2004-12-07 | 2005-12-05 | System til ekspression af melibioseoperon |
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| Country | Link |
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| US (2) | US20080305526A1 (da) |
| EP (1) | EP1824961B1 (da) |
| JP (1) | JP5080269B2 (da) |
| CN (1) | CN101090963B (da) |
| AU (1) | AU2005313530B2 (da) |
| CA (1) | CA2589823C (da) |
| DK (1) | DK1824961T3 (da) |
| ES (1) | ES2612545T3 (da) |
| HK (1) | HK1115155A1 (da) |
| IL (1) | IL183609A (da) |
| PL (1) | PL1824961T3 (da) |
| WO (1) | WO2006061173A2 (da) |
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| CN104560859B (zh) * | 2015-01-26 | 2018-03-16 | 鹭滨环保科技（上海）股份有限公司 | 一种巴氏葡萄球菌及其制备方法和在处理污水中的应用 |
| CN104560857B (zh) * | 2015-01-26 | 2018-03-16 | 鹭滨环保科技（上海）股份有限公司 | 一种茹氏短芽孢杆菌及其构建方法和在处理高浓度氨氮污水中的应用 |
| CN104560858B (zh) * | 2015-01-26 | 2018-04-13 | 鹭滨环保科技（上海）股份有限公司 | 一种红城红球菌及其构建方法和在污水处理中的应用 |
| IL261411B2 (en) | 2016-03-02 | 2024-03-01 | Lonza Ag | Improved fermentation process |
| CN113811598A (zh) | 2019-04-02 | 2021-12-17 | 丝芭博株式会社 | 重组蛋白的制备方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US5843703A (en) * | 1993-01-22 | 1998-12-01 | California Institute Of Technology | Enhanced production of toxic polypeptides in prokaryotes |
| DE4417598A1 (de) * | 1994-05-19 | 1995-12-14 | Max Planck Gesellschaft | Verwendung des Tetracyclinpromotors zur stringent regulierten Produktion von rekombinanten Proteinen in prokaryontischen Zellen |
| ATE253593T1 (de) | 1996-07-16 | 2003-11-15 | Plueckthun Andreas Prof Dr | Immunglobulin-superfamilie domainen und fragmente mit erhöhter löslichkeit |
| US6083715A (en) * | 1997-06-09 | 2000-07-04 | Board Of Regents, The University Of Texas System | Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells |
| US7094579B2 (en) | 2002-02-13 | 2006-08-22 | Xoma Technology Ltd. | Eukaryotic signal sequences for prokaryotic expression |
| EP1476551A2 (de) * | 2002-02-19 | 2004-11-17 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren zur identifikation von gewebespezifischen regulatorischen sequenzen |
-
2005
- 2005-12-05 JP JP2007544798A patent/JP5080269B2/ja active Active
- 2005-12-05 WO PCT/EP2005/013012 patent/WO2006061173A2/en active Application Filing
- 2005-12-05 AU AU2005313530A patent/AU2005313530B2/en not_active Ceased
- 2005-12-05 CA CA2589823A patent/CA2589823C/en active Active
- 2005-12-05 DK DK05817790.8T patent/DK1824961T3/da active
- 2005-12-05 CN CN2005800416001A patent/CN101090963B/zh active Active
- 2005-12-05 US US11/791,988 patent/US20080305526A1/en not_active Abandoned
- 2005-12-05 PL PL05817790T patent/PL1824961T3/pl unknown
- 2005-12-05 EP EP05817790.8A patent/EP1824961B1/en active Active
- 2005-12-05 ES ES05817790.8T patent/ES2612545T3/es active Active
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Also Published As
| Publication number | Publication date |
|---|---|
| IL183609A (en) | 2012-09-24 |
| CA2589823C (en) | 2015-03-17 |
| EP1824961A2 (en) | 2007-08-29 |
| US20080305526A1 (en) | 2008-12-11 |
| AU2005313530A1 (en) | 2006-06-15 |
| JP5080269B2 (ja) | 2012-11-21 |
| CA2589823A1 (en) | 2006-06-15 |
| ES2612545T3 (es) | 2017-05-17 |
| US8735099B2 (en) | 2014-05-27 |
| JP2008522598A (ja) | 2008-07-03 |
| CN101090963A (zh) | 2007-12-19 |
| HK1115155A1 (en) | 2008-12-24 |
| US20120077225A1 (en) | 2012-03-29 |
| WO2006061173A2 (en) | 2006-06-15 |
| CN101090963B (zh) | 2010-12-29 |
| AU2005313530B2 (en) | 2011-03-10 |
| PL1824961T3 (pl) | 2017-05-31 |
| WO2006061173A3 (en) | 2006-10-05 |
| EP1824961B1 (en) | 2016-11-02 |
| IL183609A0 (en) | 2007-09-20 |
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