DK157121B - Centrifuge for the extraction of bone proteins - Google Patents
Centrifuge for the extraction of bone proteins Download PDFInfo
- Publication number
- DK157121B DK157121B DK201481A DK201481A DK157121B DK 157121 B DK157121 B DK 157121B DK 201481 A DK201481 A DK 201481A DK 201481 A DK201481 A DK 201481A DK 157121 B DK157121 B DK 157121B
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- centrifuge
- housing
- basket
- lid
- centrifuge housing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B11/00—Feeding, charging, or discharging bowls
- B04B11/02—Continuous feeding or discharging; Control arrangements therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B3/00—Centrifuges with rotary bowls in which solid particles or bodies become separated by centrifugal force and simultaneous sifting or filtering
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
- B04B7/02—Casings; Lids
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Description
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I dansk patentskrift nr. 143 584 omhandles en fremgangsmâde til udvinding af protein fra knogler, ved hvilken knoglernes kollagen opl0seligg0res ved behand-ling med damp ved tryk st0rre end 1 atmosfære, og denne 5 fremgangsmâde er ejendommelig ved, at dampbehandlingen finder sted, eventuelt under tilsætning af vand, medens de fugtige, knuste knogler beflnder sIg i en i en tryk-beholder roterende centrifugekurv, hvori de udsættes for en centrifugalacceleration pâ mindst 5 g, idet be-10 handlingen afpasses sâledes, at der opnâs en protein-oplosning med en koncentration pâ mere end 10 vægt%, hvorpâ proteinoplosningen, om onsket, terres pâ i sig selv kendt mâde.Danish Patent No. 143,584 discloses a method for extracting bone protein in which the collagen of bones is solubilized by treatment with steam at pressures greater than 1 atmosphere, and this method is peculiar in that the steam treatment takes place, possibly under addition of water while the moist, crushed bones are soaked in a centrifuge basket rotating in a pressure vessel, subjecting them to a centrifugal acceleration of at least 5 g, adapting the treatment to obtain a protein solution with a concentration of more than 10% by weight, in which case the protein solution, if desired, is terraced in a manner known per se.
Denne fremgangsmâde har man hidtil udevet under 15 anvendelse af en centrifuge med et som trykbeholder ud-formet centrifugehus med en pâ en lodret hul aksel fastgjort centrifugekurv med perforeret periferivæg.This method has heretofore been practiced using a centrifuge with a centrifuge housing designed as a pressure vessel with a centrifuge basket with perforated peripheral wall fixed on a vertical hollow shaft.
Ved driften tilledes damp til centrifugehuset og gennem den lodrette hule aksel indpumpes til centrifugekurvens 20 indre en væske, som ved processens start bestâr af vand. Centrifugekurven var ved denne kendte konstruk-tion forsynet med et tætsluttende lâg, sâledes at der ved indpumpningen af væske gennem den hule aksel kunne skabes et tryk i centrifugekurvens indre, sterre end 25 det tryk, som fandtes i centrifugehuset uden for centrifugekurven, med andre ord en trykgradient over det lag af knuste knogler, der under driften befinder sig indvendigt pâ centrifugekurvens periferivæg. Denne trykgradient blev betragtet som nodvendig for at opnâ 30 en tilstrækkelig gennemskylning af knoglelaget og heraf betinget udvaksning af den ved behandlingen opleselig-gjorte protein.In operation, steam is supplied to the centrifuge housing and through the vertical hollow shaft a liquid is pumped to the interior of the centrifuge curve 20, which at the start of the process consists of water. In this known construction, the centrifuge curve was provided with a tightly sealing layer, so that by pumping liquid through the hollow shaft, a pressure could be created in the interior of the centrifuge curve, greater than the pressure found in the centrifuge housing outside the centrifuge curve, in other words. a pressure gradient over the layer of broken bones located inside the centrifuge curve peripheral during operation. This pressure gradient was considered necessary to obtain sufficient flushing of the bone layer and the outgrowth of the protein readable by the treatment conditional.
Den samme forestilling om nedvendigheden af ved indpumpning af væske i centrifugekurven at kunne skabe 35 et sterre tryk i centrifugekurvens indre end i den del af centr.ifugehusets indre, som ligger uden for centri-The same notion of the necessity of being able, by pumping liquid into the centrifuge curve, to create a higher pressure in the interior of the centrifuge curve than in the part of the interior of the centrifuge housing which is outside the centrifuge housing.
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2 fugekurven, genfindes i US patentskrift nr. 728 205.2 joint curve, found in U.S. Patent No. 728,205.
I dette gengives en centrifuge til anvendelse ved ud-vaskning af gélatine fra skind og huder med varmt vand.In this, a centrifuge is used for use in washing gelatin from skins and hides with warm water.
Dette vanne vand indf0res i centrifugekurven, ikke gen-5 nem en hul aksel, som tilfældet er i den i dansk patentskrift nr. 143 584 beskrevne centrifuge, men gennem et indl0b, der er f0rt centrait gennem centrifugekur-vens lâg, hvorhos der forefindes pakningsorganer mellem indlobet og lâget, sâledes at det tryk, hvormed det 10 varme vand indpumpes, kan medvirke til at tvinge vandet gennem det lag af huder eller skind, hvorfra der skal ekstraheres gélatine. I modsætning til hvad der gælder for den i dansk patentskrift nr. 143 584 omhandlede centrifuge, er centrifugehuset ikke udformet som en 15 trykbeholder, og der kan ved den pâgældende gelâtineud- vaskning derfor ikke anvendes temperaturer, som væsent- 0 ligt overstiger 100 C.This watery water is introduced into the centrifuge basket, not through a hollow shaft, as is the case in the centrifuge described in Danish Patent No. 143,584, but through an inlet which has been centrifuged through the lid of the centrifuge curve, where there are packing means. between the inlet and the lid, such that the pressure at which the hot water is pumped can help force the water through the layer of hides or skins from which gelatin is to be extracted. Contrary to the centrifuge referred to in Danish Patent No. 143,584, the centrifuge housing is not designed as a pressure vessel and, therefore, temperatures not exceeding 100 ° C can be used during the gelatin wash.
Det nævnte hidtil anvendte System til indforing af væsken under tryk i centrifugekurven under anvendel-20 se af en hul aksel eller et ovenfra kommende indlob med tilherende tætningsorganer udger imidlertid en kompli-ceret og kostbar konstruktion, der væsentligt pâvirker totalomkostningerne ved fremstillingen af en centrifuge af den omhandlede art.However, the aforementioned system used to introduce the liquid under pressure into the centrifuge curve using a hollow shaft or an overhead inlet with associated sealing means constitutes a complicated and costly construction which substantially affects the total cost of producing a centrifuge of the species in question.
25 Det har imidlertid overraskende vist sig, at det er muligt at gennemfore den ovenfor beskrevne, fra dansk patentskrift nr. 143 584 kendte, procès under anvendelse af en centrifuge med en centrifugekurv, der gennem en âbning i lâget star i forbindelse med den 0v-30 rige del af centrifugehusets indre, selv om der sâledes ikke kan tilvejebringes et storre tryk i centrifugekurven end i den 0vrige del af centrifugehuset. Herved muliggeres det, at væsken, der skal indferes i centrifugekurven, ikke behover at indpumpes gennem en hul ak-35 sel, men kan indledes pâ væsentligt simplere mâde pak-ningsfrit gennem nævnte âbning i centrifugekurvens 3However, it has surprisingly been found that it is possible to carry out the above-described process, known from Danish patent specification 143,584, using a centrifuge with a centrifuge basket which, through an opening in the lid, starts in connection with the 30 rich part of the interior of the centrifuge housing, although no greater pressure can be provided in the centrifuge curve than in the other part of the centrifuge housing. Hereby it is possible that the liquid to be inserted into the centrifuge curve does not need to be pumped through a hollow shaft, but can be introduced in a substantially simpler manner packing-free through said opening in the centrifuge curve 3
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lâg.layer.
Ârsagen til at det for fagmanden har været over-raskende, at tilfredsstillende resultater kunne opnâs ved udelukkende at anvende de ved centrifugekurvens ro-5 tation fremkomne kræfter til at give tilstrækkelig væ-skegennemstromning gennem det lag af knuste knogler, der ved centrifugeringen findes pâ indersiden af cen-trifugevæggen, er, at dette lag af knuste knogler har en sâdan karakter, at man ikke hidtil har troet det mu-10 ligt at opnâ en tilstrækkelig væskegennemskylning af laget ved de omdrejningshastigheder, der kommer i be-tragtning for de store centrifuger, som anvendes ved processer af den pâgældende art. Ogsâ ved centrifuger til ekstraktion af fedt fra knogler ved hjælp af orga-15 niske oplosningsmidler er det hidtil blevet betragtet som nodvendigt at indpumpe disse oplosningsmidler gennem en hul aksel til en med tætsluttende lâg forsynet centrifugekurv.The reason why it has been surprising to those skilled in the art that satisfactory results could be obtained by using only the forces obtained by the rotation of the spin curve to provide sufficient fluid flow through the layer of broken bones found on the inside of the spin. of the centrifuge wall, is that this layer of broken bones is such that it has not hitherto been thought possible to obtain sufficient liquid rinsing of the layer at the rotational speeds considered for the large centrifuges used in processes of the kind in question. Also, by centrifuges for extracting fat from bones by organic solvents, it has hitherto been considered necessary to pump these solvents through a hollow shaft to a tightly-sealed centrifuge basket.
Erkendelsen af, at indforing af væsken ikke be-20 hover at ske gennem en hul aksel til det indre af en lukket centrifugekurv eller af, at der iovrigt ikke be-hover at være nogen tæt afspærring mellem centrifugekurvens indre og centrifugehuset, muliggor en væsentlig forenklet konstruktion, og opfindelsen angâr i overens-25 stemmelse hermed en centrifuge til udvinding af protei-ner fra knogler og med et som trykbeholder udformet centrifugehus, der er forsynet med et aftageligt lâg og er tilsluttet en kilde for damp af tryk storre end 1 atmosfære, med en ligeledes med et aftageligt lâg for-30 synet perforeret centrifugekurv, som er fastgjort pâ en gennem centrifugehusets bund fort roterbar lodret aksel, hvorhos der fra centrifugehusets bund findes en afgangsâbning for væske, hvilken centrifuge ifolge opfindelsen er ejendommelig ved, at et gennem centrifuge-35 huset eller dets lâg indfort ror til indforsel af væske til centrifugekurvens indre udmunder over eller stræk-The recognition that the introduction of the liquid does not have to happen through a hollow shaft to the interior of a closed centrifuge basket or that otherwise there does not have to be a close barrier between the interior of the centrifuge basket and the centrifuge housing, makes for a considerably simplified Accordingly, the invention relates to a centrifuge for extracting proteins from bones and to a centrifuge housing designed as a pressure vessel having a removable lid and connected to a source of steam of pressure greater than 1 atmosphere, a perforated centrifuge basket, which is also provided with a removable cover, which is fixed to a vertical shaft rotatable through the bottom of the centrifuge housing, whereby there is a discharge opening for liquid, which centrifuge according to the invention is characterized by a centrifuge -35 the housing or its cover is inserted for the supply of liquid to the inner ends of the centrifuge basket over or stretched-
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4 ker sig pakningsfrit gennem en central âbning i centri-fugekurvens lâg.4 passes gasket-free through a central opening in the lid of the centrifuge basket.
Det er mest hensigtsmæssigt, at roret til ind-forsel af væske er fort gennem centrifugehusets lâg og 5 udmunder over centrifugekurvens centrale âbning. Nâr roret er fastgjort i lâget, vil det fjernes samtidig med dette og derfor ikke komplicere indforelse og ud-tagning af centrifugekurven i forbindelse med dennes tomning for udvasket knoglemasse og ifyldning af en 10 frisk portion knuste knogler.It is most convenient for the fluid entry tube to pass through the lid of the centrifuge housing and 5 open over the central opening of the centrifuge basket. When the rudder is secured in the lid, it will be removed at the same time and therefore will not complicate insertion and removal of the centrifuge curve in connection with its emptying of washed bone mass and loading of a fresh portion of broken bones.
Centrifugen ifolge opfindelsen illustreres nær-mere under henvisning til tegningen, hvor fig. 1 skematisk viser et anlæg til proteinud-vinding, hvilket anlæg omfatter en cen-15 trifuge ifolge opfindelsen, fig. 2 viser i snit en detalje af en ændret ud- forelsesform for centrifugen ifolge op findelsen, og fig. 3 viser et snit langs linien III-III i 20 fig. 2.The centrifuge according to the invention is further illustrated with reference to the drawing, in which: FIG. 1 schematically shows a protein extraction plant comprising a centrifuge according to the invention; FIG. 2 shows in section a detail of a modified embodiment of the centrifuge according to the invention; and FIG. 3 shows a section along the line III-III in FIG. 2nd
Det pâ fig. l viste anlæg omfatter en cenrifuge 1 med et centrifugehus 2 forsynet med et aftageligt lâg 3.The FIG. 1, a centrifuge 1 comprises a centrifuge housing 2 provided with a removable cover 3.
Centrifugehuset og lâget er konstrueret med sâ-25 danne dimensioner og forsynet med sâdanne (ikke viste) lukkeorganer, at centrifugen kan modstâ et indre tryk pâ mindst 6 ato, og det samme gælder de pâ fig. 1 viste, nedenfor beskrevne ror- og slangeforbindelser.The centrifuge housing and the lid are constructed with such dimensions and provided with such (not shown) closure means that the centrifuge can withstand an internal pressure of at least 6 ato, and the same applies to those in FIG. 1, as described below, rudder and hose connections.
Centrifugehuset er forsynet med et tilferselsror 30 4, som er sluttet til en (ikke vist) dampkilde, som er i stand til at opretholde et tryk pâ f.eks. 5 ato i centrifugehusets indre.The centrifuge housing is provided with a feed tube 30 4 which is connected to a steam source (not shown) capable of maintaining a pressure of e.g. 5 ato in the interior of the centrifuge housing.
I centrifugehuset findes en centrifugekurv 5, hvis periferivægge er forsynet med (ikke viste) perfo-35 reringer. Disse perforeringer kan i typiske tilfælde være borede huiler med en diameter pâ 2-4 mm og en ind- 5In the centrifuge housing is a centrifuge basket 5, whose peripheral walls are provided with (not shown) perforations. Typically, these perforations may be drilled hollows having a diameter of 2-4 mm and a diameter
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byrdes afstand pâ ca. 15 mm.the distance of the load is approx. 15 mm.
Centrifugekurven er i den viste udf0relsesform forsynet med en plan bund 6, som er udloseligt fast-gjort til et underst0ttelsesorgan 7, som er anbragt pâ 5 en roterbar aksel 8 koblet til et passende drivorgan, fortrinsvis en hydraulisk motor 9.In the illustrated embodiment, the centrifuge curve is provided with a flat bottom 6, which is releasably attached to a support member 7, which is mounted on a rotatable shaft 8 coupled to a suitable drive means, preferably a hydraulic motor 9.
Hvor akslen 8 er fort gennem centrifugehusets bund findes et væske- og trykbestandigt leje 10. Dette leje med tilhorende aksel 8 og understottelsesor-10 ganet 7 udgor en væsentligt enklere og billigere konstruktion end hvad der kræves, nâr indpumpning af væske skal ske under tryk gennem en hul aksel eller gennem en pakning som omhandlet i ovennævnte patent-skrifter.Where the shaft 8 is fast through the bottom of the centrifuge housing, there is a fluid and pressure resistant bearing 10. This bearing with associated shaft 8 and the support member 7 constitutes a substantially simpler and cheaper construction than is required when pumping fluid under pressure through a hollow shaft or through a gasket as provided in the aforementioned patent specifications.
15 Centrifugekurven 5 er forsynet med et aftage- ligt lâg 11, som er ringformet, sâledes at der i den centrale del findes en âbning.The centrifuge basket 5 is provided with a removable layer 11, which is annular, so that an opening is provided in the central part.
Over denne âbning udmunder et ror 12, som er fastgjort til centrifugehusets lâg 3 og fort gennem 20 dette.Above this opening, a rudder 12, which is attached to the cover 3 of the centrifuge housing, and through it 20 opens.
Roret 12 er uden for centrifugen forbundet med en slange 13, som via en rorforbindelse 14, en pumpe 15 og et yderligere ror 16 stâr i forbindelse med en tryktank 17.The tube 12 is connected outside the centrifuge to a hose 13 which is connected to a pressure tank 17 via a pipe connection 14, a pump 15 and a further pipe 16.
25 Tanken 17 er via et med afspærringsventil forsynet ror 18 forbundet med den laveste del af centri- fugehuset 2.The tank 17 is connected to the lowest part of the centrifuge housing 2 by means of a rudder 18 provided with a shut-off valve.
Til udligning af trykforskelle mellem det indre af centrifugen og tryktanken 17 findes endvidere et 30 tyndt ror 19, som forbinder de ovre dele af henholds- vis tank og centrifuge.To compensate for pressure differences between the interior of the centrifuge and the pressure tank 17, there is further provided a thin tube 19 which connects the upper parts of the tank and the centrifuge, respectively.
Ved driften af det pâgældende anlæg foretages folgende:When operating the plant in question, the following is done:
Centrifugehusets lâg âbnes og centrifugekurven 35 5 frigores fra understottelsesorganet 7 og loftes op af centrifugehuset 2.The lid of the centrifuge housing is opened and the centrifuge basket 35 5 is released from the support member 7 and lifted by the centrifuge housing 2.
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66
Centrifugekurvens lâg il aftages og en passen-de mængde knuste knogler indfores i centrifugen. Lâget 11 pâsættes atter, og centrifugekurven sænkes tilbage i centrifugehuset, hvor den fastgores til understottel- 5 sesorganet, sâledes at den vil folge dettes rotation.The centrifuge curve is removed and a suitable amount of broken bones are inserted into the centrifuge. The lid 11 is reattached and the centrifuge curve is lowered back into the centrifuge housing where it is attached to the support member so that it will follow its rotation.
Efter at centrifugehusets lâg 3 er fastspændt, tilfores der damp gennem 4 til opnaelse af et tryk i centrifugen pâ ca. 5 ato, og centrifugen bringes til at rotere f.eks. med en omdrejningshastighed pâ ca. 150 10 omdrejninger pr. minut. Herved fordeler de knuste knogler sig i et lag 20 pâ indersiden af centrifugekurvens perforerede vægge.After the cover 3 of the centrifuge housing is clamped, steam is supplied through 4 to provide a pressure in the centrifuge of approx. 5 ato and the centrifuge is rotated e.g. with a speed of approx. 150 10 rpm minute. Thereby, the broken bones are distributed in a layer 20 on the inside of the perforated walls of the centrifuge curve.
Selv om der under disse omstændigheder kan fri-gores en vis mængde proteinoplosning fra knoglerne, og-15 sâ uden at der tilfores vand, foretrækkes det at indle-de behandlingen ved gennem en ventil 21 at indfore vand som via rorforbindelsen 14, slangen 13 og roret 12 nâr det indre af centrifugekurven 5. Her fordeler vandet sig, uden at der træffes særlige foranstaltnin- 20 ger herfor, over laget 20, og der fâs en tilfredsstil-lende proteinudvaskning fra knoglerne, selv om der ik-ke, sâledes som tilfældet er ved de kendte centrifuger til dette formâl, ved pumpning tilvejebringes et foro-get tryk i centrifugekurven i forhold til trykket uden 25 for denne i centrifugehuset. Nâr den onskede vandmæng-de er indtaget, lukkes ventilen 21, og fra bunden af centrifugehuset ledes proteinoplosning til tryktanken 17, hvorfra denne oplosning recirkuleres via roret 16, pumpen 15, rorforbindelsen 14, slangen 13 og roret 30 12 til centrifugekurvens indre, hvor den atter passe- rer gennem knoglelaget og herved beriges med protein og atter cirkulerer tilbage til tanken.Although, under these circumstances, a certain amount of protein solution can be released from the bones, and so without water supply, it is preferable to initiate the treatment by introducing water through a valve 21 as through the rudder compound 14, the hose 13 and the tube 12 reaches the interior of the centrifuge curve 5. Here the water distributes, without taking special measures 20, over the layer 20, and satisfactory protein washout from the bones, although not, as is the case is by the known centrifuges for this purpose, by pumping an increased pressure in the centrifuge curve relative to the pressure outside it is provided in the centrifuge housing. When the desired volume of water is ingested, the valve 21 is closed and from the bottom of the centrifuge housing, protein solution is fed to the pressure tank 17, from which this solution is recycled via the tube 16, the pump 15, the rudder connection 14, the hose 13 and the tube 30 12 to the interior of the centrifuge basket. again passes through the bone layer and thereby enriched with protein and again circulates back to the tank.
Denne procès fortsættes til væsken har antaget den optimale proteinkoncentration, hvilket f.eks. kon-35 stateres refraktometrisk. Pâ dette tidspunkt âbnes en ventil 22, hvorigennem proteinoplosningen udtages.This process is continued until the liquid has assumed the optimum protein concentration, which e.g. refractometric. At this point, a valve 22 opens through which the protein solution is withdrawn.
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7 trykket i centrifugen udloses, og centrifugen âbnes og t0mmes.7 the pressure in the centrifuge is released and the centrifuge is opened and emptied.
Alternativt kan da efter udtagningen af f0rste portion proteinopl0sning gennem 22 atter indtages 5 frisk vand gennem 21, og processen gentages med samme portion knogler.Alternatively, after taking the first portion of protein solution through 22 again, 5 fresh water can be taken through 21 and the process repeated with the same portion of bones.
Ved den udforelsesform for centrifugen ifolge opfindelsen, af hvilken en detalje er vist i fig. 2 og 3, er det pâ fig, l viste ror 12 og den dér viste 10 slange 13 erstattet af andre konstruktionsdele.In the embodiment of the centrifuge according to the invention, a detail of which is shown in FIG. 2 and 3, the tube 12 shown in FIG. 1 and the tube 13 shown there are replaced by other structural members.
Ved denne udforelsesform er centrifugelâget 3 forsynet med en loftearm 23 omfattende to vanger 24 af hvilke kun den ene ses pâ fig. 2.In this embodiment, the centrifuge cover 3 is provided with a ceiling arm 23 comprising two vanes 24, only one of which is shown in FIG. 2nd
Loftearmen 2 3 er fastgjort til lâget 3 og 15 aktiveres pneumatisk eller hydraulisk, som det fremgâr af fig, 2.The loft arm 2 3 is attached to the cover 3 and 15 is actuated pneumatically or hydraulically, as shown in Fig. 2.
Loftearmen er underst0ttet drejeligt i et leje 25, hvis konstruktion fremgâr af det i fig. 3 viste snit.The loft arm is pivotally supported in a bearing 25, the construction of which is apparent from that shown in FIG. 3.
20 Loftearmen 25 bæres af en konsol 26 fast gjort til centrifugehuset 2 og omfattende to vanger 27 af hvilke begge ses pâ fig. 3.The loft arm 25 is supported by a bracket 26 fixed to the centrifuge housing 2 and comprising two vanes 27, both of which are shown in FIG. Third
Nâr loftearmen hæves, drejer vangerne 24 sig ved de pâ fig. 3 viste bosninger 28.When the loft arm is raised, the vanes 24 rotate at those shown in FIG. 3 bushings 28.
25 Mellem vangerne 24 findes et ror 29, hvis ene ende udmunder centrait i centrifugehuset, dvs. over den i centrifugekurvens lâg værende âbning. Rorets anden ende er forbundet med en central passage i lejet 25, hvilken passage 30 atter stâr i forbindelse med en 30 rortilslutning 31. Nâr loftearmen aktiveres til hæv-ning af lâget, folger roret 29 lâgets bevægelser, hvilken bevægelighed muliggores ved anvendelse af væ-sketætte pakninger 32.25 Between rods 24 there is a rudder 29, one end of which opens into the centrrait of the centrifuge housing, ie. above the opening in the lid of the centrifuge basket. The other end of the rudder is connected to a central passage in the bearing 25, which passage 30 again communicates with a rudder connection 31. When the ceiling arm is actuated to raise the lid, the rudder 29 follows the movement of the bed, which is made possible by the use of the wall. skeleton gaskets 32.
Ved anvendelse af centrifugen i denne udforel-35 sesform tilsluttes rorforbindelsen 14 rortilslutnin-gen 31.Using the centrifuge in this embodiment, the rudder connection 14 is connected to the rudder connection 31.
Claims (3)
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK201481A DK157121C (en) | 1981-05-06 | 1981-05-06 | Centrifuge for the extraction of bone proteins |
| IE106082A IE52843B1 (en) | 1981-05-06 | 1982-05-05 | Centrifuge for recovering proteins from bones |
| EP19820302323 EP0069449B1 (en) | 1981-05-06 | 1982-05-06 | Centrifuge for recovering proteins from bones |
| DE8282302323T DE3273113D1 (en) | 1981-05-06 | 1982-05-06 | Centrifuge for recovering proteins from bones |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK201481A DK157121C (en) | 1981-05-06 | 1981-05-06 | Centrifuge for the extraction of bone proteins |
| DK201481 | 1981-05-06 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DK201481A DK201481A (en) | 1982-11-07 |
| DK157121B true DK157121B (en) | 1989-11-13 |
| DK157121C DK157121C (en) | 1990-04-16 |
Family
ID=8109477
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK201481A DK157121C (en) | 1981-05-06 | 1981-05-06 | Centrifuge for the extraction of bone proteins |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0069449B1 (en) |
| DE (1) | DE3273113D1 (en) |
| DK (1) | DK157121C (en) |
| IE (1) | IE52843B1 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3740411C2 (en) * | 1987-11-28 | 1996-11-14 | Heinkel Ind Zentrifugen | Inverting filter centrifuge |
| US6387070B1 (en) | 1999-09-17 | 2002-05-14 | Nuvasive, Inc. | Bone slurry recovery |
| JP5633805B2 (en) * | 2010-11-26 | 2014-12-03 | 日立工機株式会社 | centrifuge |
| CN107519664A (en) * | 2017-09-21 | 2017-12-29 | 郑州大学 | A kind of efficient traditional Chinese medicine extyaction device |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4588C (en) * | A. FeSCA, Maschinenfabrikant, in Berlin, Chausseestrafse 35 | Steam deck device on centrifuges with a catch device for the condensation water | ||
| FR347408A (en) * | 1904-10-25 | 1905-03-09 | Oil And Waste Saving Machine Company | Centrifugal machine for fat extraction |
| GB377560A (en) * | 1931-01-08 | 1932-07-28 | Separator Ab | Connecting device for the supply and the discharge pipes of centrifugal separators |
| DK143584C (en) * | 1978-03-22 | 1982-03-29 | Lildal J A S | PROCEDURE FOR THE EXTRACTION OF PROTEIN FROM BONE AND THE EXERCISE OF THE PROCEDURE |
-
1981
- 1981-05-06 DK DK201481A patent/DK157121C/en not_active IP Right Cessation
-
1982
- 1982-05-05 IE IE106082A patent/IE52843B1/en unknown
- 1982-05-06 EP EP19820302323 patent/EP0069449B1/en not_active Expired
- 1982-05-06 DE DE8282302323T patent/DE3273113D1/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| EP0069449B1 (en) | 1986-09-10 |
| EP0069449A3 (en) | 1984-06-06 |
| DK157121C (en) | 1990-04-16 |
| EP0069449A2 (en) | 1983-01-12 |
| DE3273113D1 (en) | 1986-10-16 |
| DK201481A (en) | 1982-11-07 |
| IE52843B1 (en) | 1988-03-16 |
| IE821060L (en) | 1982-11-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PBP | Patent lapsed |