DK1543438T3 - Fremgangsmåde og systemer til estimering af smelte-temperaturen (Tm) for polynukleotidmolekyler - Google Patents

Fremgangsmåde og systemer til estimering af smelte-temperaturen (Tm) for polynukleotidmolekyler

Info

Publication number
DK1543438T3
DK1543438T3 DK03754518T DK03754518T DK1543438T3 DK 1543438 T3 DK1543438 T3 DK 1543438T3 DK 03754518 T DK03754518 T DK 03754518T DK 03754518 T DK03754518 T DK 03754518T DK 1543438 T3 DK1543438 T3 DK 1543438T3
Authority
DK
Denmark
Prior art keywords
methods
melting temperature
systems
algorithms
estimating
Prior art date
Application number
DK03754518T
Other languages
Danish (da)
English (en)
Inventor
Richard Owczarzy
Joseph Alan Walder
Lingyan Huang
Mark Aaron Behlke
Original Assignee
Integrated Dna Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Integrated Dna Tech Inc filed Critical Integrated Dna Tech Inc
Application granted granted Critical
Publication of DK1543438T3 publication Critical patent/DK1543438T3/da

Links

Classifications

    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/20Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biotechnology (AREA)
  • Evolutionary Biology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK03754518T 2002-09-12 2003-09-12 Fremgangsmåde og systemer til estimering af smelte-temperaturen (Tm) for polynukleotidmolekyler DK1543438T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41066302P 2002-09-12 2002-09-12
PCT/US2003/028664 WO2004025257A2 (en) 2002-09-12 2003-09-12 Methods and systems for estimating the melting temperature (tm) for polynucleotide molecules

Publications (1)

Publication Number Publication Date
DK1543438T3 true DK1543438T3 (da) 2009-08-03

Family

ID=31994175

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03754518T DK1543438T3 (da) 2002-09-12 2003-09-12 Fremgangsmåde og systemer til estimering af smelte-temperaturen (Tm) for polynukleotidmolekyler

Country Status (9)

Country Link
US (1) US6889143B2 (ja)
EP (1) EP1543438B1 (ja)
JP (1) JP4455334B2 (ja)
AT (1) ATE430964T1 (ja)
AU (1) AU2003272340B2 (ja)
CA (1) CA2498414A1 (ja)
DE (1) DE60327541D1 (ja)
DK (1) DK1543438T3 (ja)
WO (1) WO2004025257A2 (ja)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2118651A4 (en) * 2007-01-05 2012-11-14 Integrated Dna Tech Inc METHOD FOR ESTIMATING THE FUSION TEMPERATURE OF A NUCLEIC ACID IN BUFFERS CONTAINING BIVALENT IONS
JP5294380B2 (ja) * 2007-12-05 2013-09-18 学校法人東京理科大学 核酸構造解析装置及びプログラム
US9542526B2 (en) * 2009-03-10 2017-01-10 Canon U.S. Life Sciences, Inc. Method and system for temperature correction in thermal melt analysis
WO2011060014A1 (en) 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
US9081737B2 (en) 2010-08-02 2015-07-14 Integrated Dna Technologies, Inc. Methods for predicting stability and melting temperatures of nucleic acid duplexes
US11330838B2 (en) 2019-07-19 2022-05-17 R. J. Reynolds Tobacco Company Holder for aerosol delivery device with detachable cartridge

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3019994B2 (ja) 1987-11-30 2000-03-15 ユニバーシティ オブ アイオワ リサーチ ファウンデーション 新規なオリゴデオキシヌクレオチド、それを使用した標的遺伝子の発現をブロックする方法、及び新規なオリゴデオキシヌクレオチド並びにそれを使用した標的遺伝子の発現を阻止する方法
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
JP3785517B2 (ja) * 1997-06-05 2006-06-14 東ソー株式会社 核酸融解温度測定法
EP1103910A1 (en) * 1999-11-24 2001-05-30 Schütz, Ekkehard Method of automatically selecting Oligonucleotide Hybridization probes

Also Published As

Publication number Publication date
EP1543438B1 (en) 2009-05-06
ATE430964T1 (de) 2009-05-15
JP4455334B2 (ja) 2010-04-21
EP1543438A4 (en) 2006-04-12
WO2004025257A3 (en) 2004-08-12
JP2006502711A (ja) 2006-01-26
AU2003272340B2 (en) 2009-08-13
US20040115705A1 (en) 2004-06-17
CA2498414A1 (en) 2004-03-25
EP1543438A2 (en) 2005-06-22
WO2004025257A2 (en) 2004-03-25
DE60327541D1 (de) 2009-06-18
AU2003272340A1 (en) 2004-04-30
US6889143B2 (en) 2005-05-03

Similar Documents

Publication Publication Date Title
JP7112456B2 (ja) バリアント検出のための方法
Tost et al. Analysis of gene-specific DNA methylation patterns by Pyrosequencing® technology
DK1171632T3 (da) Nucleinsyreligand-inhibitorer af DNA-polymeraser
US9081737B2 (en) Methods for predicting stability and melting temperatures of nucleic acid duplexes
DK0833944T3 (da) Nucleinsyreligander som binder til og inhiberer DNA-polymeraser
WO2007130519A3 (en) Viral nucleic acid microarray and method of use
CN102625850A (zh) 多重核酸检测方法和系统
EP1594975A2 (en) Multiplex amplification of polynucleotides
JP6971276B2 (ja) クランプオリゴヌクレオチドを利用する核酸増幅方法
US20160024546A1 (en) Methods for Amplification of Nucleic Acids Utilizing a Circularized Template Prepared from a Target Nucleic Acid
Kalendar et al. Palindromic sequence-targeted (PST) PCR: a rapid and efficient method for high-throughput gene characterization and genome walking
EP3555316B1 (en) Modified multiplex and multistep amplification reactions and reagents therefor
WO2008147930A3 (en) Methods and kits for linking polymorphic sequences to expanded repeat mutations
JP2016516409A (ja) 固体支持体上での核酸増幅方法
EP1483406A1 (en) Headloop dna amplification
Sheng et al. Single-cell RNA-seq by multiple annealing and tailing-based quantitative single-cell RNA-seq (MATQ-Seq)
Igartua et al. Targeted enrichment of specific regions in the human genome by array hybridization
DK1543438T3 (da) Fremgangsmåde og systemer til estimering af smelte-temperaturen (Tm) for polynukleotidmolekyler
JP2013515458A5 (ja)
Kolmodin et al. Polymerase chain reaction: basic principles and routine practice
WO2008086322A3 (en) Method for estimating melting temperature of a nucleic acid in buffers containing divalent ions
US20080124769A1 (en) Apparatus and method for reducing non-specific amplification in multiplex pcr
Tsigenopoulos et al. Eleven new microsatellites of the sea bass (Dicentrarchus labrax L.)
Wojdacz et al. Melting curve assays for DNA methylation analysis
WO2009051803A3 (en) Microarray-based gene copy number analyses