DK1504117T3 - Fremgangsmåde til at generere sekvensspecifikke proteaser ved målrettet evolution - Google Patents

Fremgangsmåde til at generere sekvensspecifikke proteaser ved målrettet evolution

Info

Publication number
DK1504117T3
DK1504117T3 DK03727460T DK03727460T DK1504117T3 DK 1504117 T3 DK1504117 T3 DK 1504117T3 DK 03727460 T DK03727460 T DK 03727460T DK 03727460 T DK03727460 T DK 03727460T DK 1504117 T3 DK1504117 T3 DK 1504117T3
Authority
DK
Denmark
Prior art keywords
generating sequence
specific proteases
proteases
targeted evolution
sequence
Prior art date
Application number
DK03727460T
Other languages
Danish (da)
English (en)
Inventor
Ulrich Haupts
Jan Tebbe
Peter Scholz
Jens Pilling
Susanne Werner
Markus Rarbach
Andre Koltermann
Ulrich Kettling
Original Assignee
Direvo Biotech Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Direvo Biotech Ag filed Critical Direvo Biotech Ag
Application granted granted Critical
Publication of DK1504117T3 publication Critical patent/DK1504117T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
DK03727460T 2002-05-10 2003-05-09 Fremgangsmåde til at generere sekvensspecifikke proteaser ved målrettet evolution DK1504117T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02010576A EP1361284A1 (de) 2002-05-10 2002-05-10 Verfahren zur Herstellung von sequenzspezifischen Proteasen durch zielgerichtete Evolution und deren Verwendung
PCT/EP2003/004864 WO2003095670A2 (en) 2002-05-10 2003-05-09 Process for generating sequence-specific proteases by directed evolution and use thereof

Publications (1)

Publication Number Publication Date
DK1504117T3 true DK1504117T3 (da) 2007-11-12

Family

ID=29225644

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03727460T DK1504117T3 (da) 2002-05-10 2003-05-09 Fremgangsmåde til at generere sekvensspecifikke proteaser ved målrettet evolution

Country Status (9)

Country Link
EP (2) EP1361284A1 (de)
JP (1) JP4448772B2 (de)
AT (1) ATE367450T1 (de)
AU (2) AU2003233314A1 (de)
CA (1) CA2485452A1 (de)
DE (1) DE60315014T2 (de)
DK (1) DK1504117T3 (de)
ES (1) ES2289288T3 (de)
WO (1) WO2003095670A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7939304B2 (en) 2002-10-02 2011-05-10 Catalyst Biosciences, Inc. Mutant MT-SP1 proteases with altered substrate specificity or activity
ATE526399T1 (de) 2003-06-18 2011-10-15 Bayer Pharma AG Neue biologische einheiten und deren verwendung
WO2005087947A2 (en) * 2004-03-09 2005-09-22 Meyer Pharmaceuticals Llc Bioengineered proteolytic enzymes with enhanced specificity for cytokine receptors
CA2562729C (en) 2004-04-12 2013-11-12 Sandra Waugh Ruggles Cleavage of vegf and vegf receptor by wildtype and mutant mt-sp1
EP1827486A2 (de) * 2004-12-22 2007-09-05 Direvo Biotech AG Gezielte verwendung von technisch manipulierten enzymen
KR101393946B1 (ko) 2005-10-21 2014-05-12 카탈리스트 바이오사이언시즈, 인코포레이티드 보체 활성화를 억제하는 변형된 프로테아제
KR101778174B1 (ko) 2006-07-05 2017-09-13 카탈리스트 바이오사이언시즈, 인코포레이티드 프로테아제 스크리닝 방법 및 이에 의해 확인된 프로테아제
TWI465247B (zh) 2008-04-11 2014-12-21 Catalyst Biosciences Inc 經修飾的因子ｖｉｉ多肽和其用途
CN103429738A (zh) 2011-03-24 2013-12-04 旭硝子株式会社 裂殖酵母属酵母的转化体、该转化体的制造方法、β-葡糖苷酶的制造方法及纤维素的降解方法
CN111019925B (zh) * 2018-10-10 2022-08-23 上饶市康可得生物科技有限公司 筛选蛋白酶变体的方法以及获得的蛋白酶变体
JP7386552B2 (ja) * 2018-10-10 2023-11-27 上饒市康可得生物科技有限公司 プロテアーゼバリアントのスクリーニング方法および得られたプロテアーゼバリアント
US20210069306A1 (en) 2019-08-15 2021-03-11 Catalyst Biosciences, Inc. Modified factor vii polypeptides for subcutaneous administration and on-demand treatment

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9718591D0 (en) * 1997-09-03 1997-11-05 Zeneca Ltd Methods
US6680178B2 (en) * 2000-06-02 2004-01-20 The Regents Of The University Of California Profiling of protease specificity using combinatorial fluorogenic substrate libraries

Also Published As

Publication number Publication date
ES2289288T3 (es) 2008-02-01
CA2485452A1 (en) 2003-11-20
EP1361284A1 (de) 2003-11-12
ATE367450T1 (de) 2007-08-15
WO2003095670A3 (en) 2004-04-01
AU2009200749B2 (en) 2011-08-25
AU2009200749A1 (en) 2009-03-19
DE60315014T2 (de) 2008-04-10
EP1504117B1 (de) 2007-07-18
AU2003233314A1 (en) 2003-11-11
JP2005525123A (ja) 2005-08-25
WO2003095670A2 (en) 2003-11-20
DE60315014D1 (de) 2007-08-30
EP1504117A2 (de) 2005-02-09
JP4448772B2 (ja) 2010-04-14

Similar Documents

Publication Publication Date Title
DK1504117T3 (da) Fremgangsmåde til at generere sekvensspecifikke proteaser ved målrettet evolution
SE0100901D0 (sv) New composition
HK1076541A1 (en) Electro-osmotic pumps and micro-channels
SG158138A1 (en) Bonding wire cleaning unit with a chamber and an energy source and method of wire bonding using such cleaning unit
ATE396551T1 (de) Verfahren zur verringerung des stromverbrauchs in bluetooth- und cdma-betriebsarten
ATE338571T1 (de) Verfahren zur oberflächenmodifizierung
EP2292743A3 (de) Perhydrolase
WO2005041752A3 (en) Bone plate and method for using bone plate
WO2005076777A3 (en) Chemical and biological warfare decontaminating solution using peracids and germinants in microemulsions, process and product thereof
EA200400252A1 (ru) Стабильная полиморфная модификация флибансерина, способ её промышленного получения и её применение для получения лекарственных средств
NO20012165L (no) Strålingskildemodul og renseapparatur for denne
ITMI20021737A1 (it) Processo di purificazione di nanotubi di carbonio.
WO2006067163A3 (en) Blood pressure lowering oligopeptides in a single enzymatic step
PL1651814T3 (pl) Sposób wytwarzania papieru
ATE455590T1 (de) Hochdruckverfahren
UA93067C2 (ru) Способ раструбного формования конца трубки из термопластичного материала, в частности, из полиолефинового материала, которая используется для текучей среды повышенного давления
DE50013600D1 (de) Operationswerkzeug
HUP0300065A2 (en) Material processing by repeated solvent expansion-contraction
BR0117189A (pt) Processo para tratar um meio aquoso que contém oxima de ciclohexanona e ciclohexanona
DK1274426T3 (da) Anvendelse af thioamidoxazolidinoner til behandling af knogleresorption og osteoporose
EA200401536A1 (ru) Способ и промежуточные соединения для получения ненуклеозидных ингибиторов ревертазы вируса вич-1
SE0201762D0 (sv) Ozone Solutions
SE0201430L (sv) Tryckcellspress med gripanordning samt förfarande och användning vid tryckcellspress
ITMI20041271A1 (it) Processo di preparazione di gabapentina
BR0002664A (pt) Processo e aparelho para comprimir passivamenteum pulso óptico de transformação limitadadentro de um sistema de transmissão óptica