DK142880B - Process for preparing alcohol from cellulose material. - Google Patents
Process for preparing alcohol from cellulose material. Download PDFInfo
- Publication number
- DK142880B DK142880B DK111576AA DK111576A DK142880B DK 142880 B DK142880 B DK 142880B DK 111576A A DK111576A A DK 111576AA DK 111576 A DK111576 A DK 111576A DK 142880 B DK142880 B DK 142880B
- Authority
- DK
- Denmark
- Prior art keywords
- flask
- cellulose material
- cellulase
- alcohol
- flasks
- Prior art date
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims description 24
- 239000001913 cellulose Substances 0.000 title claims description 6
- 229920002678 cellulose Polymers 0.000 title claims description 6
- 239000000463 material Substances 0.000 title claims description 6
- 238000004519 manufacturing process Methods 0.000 title claims description 3
- 108010059892 Cellulase Proteins 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 229940106157 cellulase Drugs 0.000 claims description 7
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 230000001461 cytolytic effect Effects 0.000 claims description 2
- 244000005700 microbiome Species 0.000 claims description 2
- 235000019441 ethanol Nutrition 0.000 description 10
- 239000000203 mixture Substances 0.000 description 6
- 235000016709 nutrition Nutrition 0.000 description 4
- 239000000706 filtrate Substances 0.000 description 3
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 241000223261 Trichoderma viride Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Description
142880 2 cellulosematerialer benytter flere trin ved først at forsukre cellulose enzymatisk med en cellulase til frembringelse af glucose, som dernæst adskilles fra det til forsukringen benyttede udgangsmateriale og særskilt underkastes et andet trin med forgæring i nærværelse af en alkoholfrembringende organisme til dannelse af alkohol.Cellulose materials utilize several steps by first enzymatically sacrificing cellulose with a cellulase to produce glucose, which is then separated from the starting material used for suction, and separately subjected to a second step of fermentation in the presence of an alcohol-producing organism to form alcohol.
Den foreliggende fremgangsmåde udgør en forbedring i forhold til fremgangsmåden ifølge hovedpatentet ved at tilvejebringe en yderligere forøgelse i udbyttet af det ønskede slutprodukt, ethylalkohol.The present process represents an improvement over the process of the main patent by providing a further increase in the yield of the desired final product, ethyl alcohol.
Dette opnås ved et særligt valg af den ene enzymkilde, nemlig cellula-sen, som benyttes ved fremgangsmåden ifølge hovedpatentet.This is achieved by a particular choice of the one enzyme source, namely the cellulase, which is used in the process of the main patent.
I overensstemmelse hermed angår den foreliggende opfindelse en fremgangsmåde ifølge patent nr. 140764 til fremstilling af alkohol, hvilken fremgangsmåde er ejendommelig ved, at man som cellulase-kilden benytter hele den vandige næringskultur hidrørende fra dyrkningen af en cellulolytisk mikroorganisme i et vandigt næringsmedium i nærværelse af et cellulosemateriale, uden adskillelse af nogen komponent fra denne næringskultur.Accordingly, the present invention relates to a process according to Patent No. 140764 for the production of alcohol which is characterized in that as the source of cellulase the whole aqueous nutritional culture derived from the cultivation of a cellulolytic microorganism in an aqueous nutrient medium is used in the presence of a cellulose material, without separating any component from this nutritional culture.
Fremgangsmåden ifølge den foreliggende opfindelse og den herved i forhold til fremgangsmåden ifølge hovedpatentet opnåede yderligere forbedring belyses tydeligere ved følgende eksempel.The process of the present invention and the further improvement thus obtained in relation to the method of the main patent are illustrated more clearly by the following example.
Eksempel I to særskilte rystekolber dyrkes Trichoderma viride QM 9414 (ATCC 26.921) aerobt ved 30°C i et tidsrum på 6 dage. Hver kolbe indeholder 100 ml af et identisk konventionelt næringsmedium, der indeholder cellulosepulver som carbonkilden. Under dyrkningen indstilles pH to gange om dagen på 5,4. Ved afslutningen af 6 dages perioden filtreres indholdet af den ene af kolberne (A) til frembringelse af et kulturfiltrat indeholdende cellulaseenzymkomplekset. Indholdet af den anden kolbe (B) filtreres ikke. Begge de således frembragte cellulaseholdige materialer benyttes ved fremgangsmåden ifølge hovedpatentet, således som nedenfor beskrevet.Example In two separate shake flasks, Trichoderma viride QM 9414 (ATCC 26.921) is grown aerobically at 30 ° C for a period of 6 days. Each flask contains 100 ml of an identical conventional nutrient medium containing cellulose powder as the carbon source. During cultivation, the pH is adjusted to 5.4 twice a day. At the end of the 6 day period, the contents of one of the flasks (A) are filtered to produce a culture filtrate containing the cellulase enzyme complex. The contents of the second flask (B) are not filtered. Both of the cellulase-containing materials thus produced are used in the process of the main patent, as described below.
I to steriliserede 100 ml kolber anbringes aseptisk og hver for sig 5 g steriliseret cellulosepulver (300 mesh, indeholdende 95 vægtprocent cellulose). I den ene af disse kolber (C) tilsættes 45 ml af ovennævnte kulturfiltrat fra kolben (A). I den anden af disse kolber (D) tilsættes 45 ml af den ovenfor omtalte grundigt omrørte, men ufiltrerede næringskultur fra kolben (B), det vil altså sige hele den vandige kul- 142880 3 tunnasse uden adskillelse af nogen komponenter derfra (det vil altså sige hele næringskultursuppen). Dernæst tilsættes til hver af kolberne (C) og (D) 5 ml af en steriliseret opløsning indeholdende følgende bestanddele :In two sterilized 100 ml flasks, aseptically and separately 5 g of sterilized cellulose powder (300 mesh containing 95% by weight of cellulose) is placed separately. To one of these flasks (C), add 45 ml of the above culture filtrate from flask (A). In the second of these flasks (D), 45 ml of the above-mentioned well-stirred but unfiltered nutritional culture from the flask (B) is added, that is, the entire aqueous coal tunnel without separating any components therefrom (i.e. say the whole nutritional culture soup). Next, add to each of flasks (C) and (D) 5 ml of a sterilized solution containing the following ingredients:
Asparagin 125 mg.Asparagine 125 mg.
KH2P04 50 mg.KH2PO4 50 mg.
MgS04 . 7H20 150 mg.MgS04. 7H 2 O 150 mg.
Gærekstrakt 10 mg.Yeast extract 10 mg.
Destilleret vand 5 mg.Distilled water 5 mg.
De herved fremkomne blandinger i hver kolbe indstilles på pH 4,0 efter behov. Derefter tilsættes 2 platinøjefulde af saccharo-myces cerevisiae direkte fra en skråagar deraf til hver af kolberne, og blandingen tillades at omsættes anaerobt ved ca. 30°C i 96 timer.The resulting mixtures in each flask are adjusted to pH 4.0 as needed. Then, 2 platinum eyes of Saccharomyces cerevisiae are added directly from one oblique agar thereof to each of the flasks, and the mixture is allowed to react anaerobically at ca. 30 ° C for 96 hours.
Når reaktionsblandingerne analyseres, konstateres dannelse af 1,2 g ethanol i blandingen fra kolben (C), hvorimod der iagttages dannelsen af 1,4 g ethanol i blandingen fra kolben (D). Når omsætningen fortsættes i yderligere 96 timer, viser det sig, at blandingen fra kolben (C) indeholder 2,0 g ethanol, medens blandingen fra kolben (D) indeholder 2,3 g ethanol.When analyzing the reaction mixtures, formation of 1.2 g of ethanol in the mixture from the flask (C) is observed, whereas the formation of 1.4 g of ethanol in the mixture from the flask (D) is observed. When the reaction is continued for an additional 96 hours, it appears that the mixture from the flask (C) contains 2.0 g of ethanol, while the mixture from the flask (D) contains 2.3 g of ethanol.
En sammenligning af de således opnåede resultater viser, , at man ved brugen af hele kulturmediet (kolben (B)) som cellulase- kilden sammenlignet med resultater opnået ved brug af kulturfiltratet (kolben (C)) som cellulasekilden opnår en bemærkelsesværdig forbedring i udbyttet af ethanol, nemlig 2,3 g sammenlignet med 2,0 g, hvilket indicerer en forøgelse på 15% i udbyttet af ønsket produkt, ethylal-kohol.A comparison of the results thus obtained shows that by using the entire culture medium (flask (B)) as the source of cellulase compared with results obtained using the culture filtrate (flask (C)) as the source of cellulase, a remarkable improvement in the yield of ethanol, namely 2.3 g compared to 2.0 g, indicating a 15% increase in the yield of desired product, ethyl alcohol.
Claims (1)
Priority Applications (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA235,874A CA1055861A (en) | 1974-09-20 | 1975-09-19 | Manufacture of alcohol from cellulosic materials |
| DE2609551A DE2609551C2 (en) | 1975-09-08 | 1976-03-08 | Production of alcohol from cellulose |
| GB9894/76A GB1497958A (en) | 1975-09-08 | 1976-03-12 | Manufacture of ethanol from cellulosic materials |
| DK111576AA DK142880B (en) | 1975-09-08 | 1976-03-15 | Process for preparing alcohol from cellulose material. |
| BE165169A BE839573R (en) | 1975-09-08 | 1976-03-15 | MANUFACTURE OF ALCOHOL FROM CELLULOSIC MATERIALS |
| NL7602796A NL7602796A (en) | 1975-09-08 | 1976-03-17 | PROCESS FOR PREPARING ALCOHOL FROM CELL LULOSE MATERIAL. |
| IT21315/76A IT1064799B (en) | 1975-09-08 | 1976-03-17 | PROCEDURE FOR THE PRODUCTION OF ALCOHOL WITH HIGH YIELDS FROM CELLULOSE MATERIALS |
| SE7603394A SE436756B (en) | 1975-09-08 | 1976-03-18 | SEE IN THE MANUFACTURE OF ETHANOL FROM CELLULOSAMATERIAL THROUGH THE IMPACT OF CELLULAS AND AN ALCOHOL PRODUCING MICROORGANISM |
| JP51029748A JPS6043118B2 (en) | 1975-09-08 | 1976-03-18 | How to make alcohol from cellulose |
| FI760738A FI760738A (en) | 1975-09-08 | 1976-03-19 | |
| NO760977A NO145101C (en) | 1975-09-08 | 1976-03-19 | PROCEDURE FOR THE PREPARATION OF ETHANOL FROM CELLULOSE SUBSTANCES. |
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US61073175 | 1975-09-08 | ||
| US05/610,731 US3990944A (en) | 1974-09-20 | 1975-09-08 | Manufacture of alcohol from cellulosic materials using plural ferments |
| DK422375AA DK140764B (en) | 1974-09-20 | 1975-09-19 | Process for producing alcohol from cellulosic material. |
| DK422375 | 1975-09-19 | ||
| DK111576 | 1976-03-15 | ||
| DK111576AA DK142880B (en) | 1975-09-08 | 1976-03-15 | Process for preparing alcohol from cellulose material. |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DK111576A DK111576A (en) | 1977-03-09 |
| DK142880B true DK142880B (en) | 1981-02-16 |
| DK142880C DK142880C (en) | 1981-08-31 |
Family
ID=27221119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK111576AA DK142880B (en) | 1974-09-20 | 1976-03-15 | Process for preparing alcohol from cellulose material. |
Country Status (10)
| Country | Link |
|---|---|
| JP (1) | JPS6043118B2 (en) |
| BE (1) | BE839573R (en) |
| DE (1) | DE2609551C2 (en) |
| DK (1) | DK142880B (en) |
| FI (1) | FI760738A (en) |
| GB (1) | GB1497958A (en) |
| IT (1) | IT1064799B (en) |
| NL (1) | NL7602796A (en) |
| NO (1) | NO145101C (en) |
| SE (1) | SE436756B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101821397A (en) * | 2007-10-12 | 2010-09-01 | 丹尼斯科美国公司 | From organism of fermentation, improve the method and composition that organism produces |
| CN102325889A (en) * | 2008-12-19 | 2012-01-18 | 诺维信股份有限公司 | Increase the method for cellulose materials hydrolysis |
-
1976
- 1976-03-08 DE DE2609551A patent/DE2609551C2/en not_active Expired
- 1976-03-12 GB GB9894/76A patent/GB1497958A/en not_active Expired
- 1976-03-15 DK DK111576AA patent/DK142880B/en unknown
- 1976-03-15 BE BE165169A patent/BE839573R/en active
- 1976-03-17 NL NL7602796A patent/NL7602796A/en not_active Application Discontinuation
- 1976-03-17 IT IT21315/76A patent/IT1064799B/en active
- 1976-03-18 SE SE7603394A patent/SE436756B/en not_active IP Right Cessation
- 1976-03-18 JP JP51029748A patent/JPS6043118B2/en not_active Expired
- 1976-03-19 FI FI760738A patent/FI760738A/fi not_active Application Discontinuation
- 1976-03-19 NO NO760977A patent/NO145101C/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE2609551A1 (en) | 1977-03-10 |
| SE7603394L (en) | 1977-03-09 |
| DE2609551C2 (en) | 1982-06-03 |
| IT1064799B (en) | 1985-02-25 |
| NO145101B (en) | 1981-10-05 |
| JPS5234981A (en) | 1977-03-17 |
| DK142880C (en) | 1981-08-31 |
| GB1497958A (en) | 1978-01-12 |
| DK111576A (en) | 1977-03-09 |
| SE436756B (en) | 1985-01-21 |
| JPS6043118B2 (en) | 1985-09-26 |
| NL7602796A (en) | 1977-03-10 |
| NO145101C (en) | 1982-01-13 |
| BE839573R (en) | 1976-09-15 |
| FI760738A (en) | 1977-03-06 |
| NO760977L (en) | 1977-03-09 |
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