SE436756B - SEE IN THE MANUFACTURE OF ETHANOL FROM CELLULOSAMATERIAL THROUGH THE IMPACT OF CELLULAS AND AN ALCOHOL PRODUCING MICROORGANISM - Google Patents
SEE IN THE MANUFACTURE OF ETHANOL FROM CELLULOSAMATERIAL THROUGH THE IMPACT OF CELLULAS AND AN ALCOHOL PRODUCING MICROORGANISMInfo
- Publication number
- SE436756B SE436756B SE7603394A SE7603394A SE436756B SE 436756 B SE436756 B SE 436756B SE 7603394 A SE7603394 A SE 7603394A SE 7603394 A SE7603394 A SE 7603394A SE 436756 B SE436756 B SE 436756B
- Authority
- SE
- Sweden
- Prior art keywords
- ethanol
- manufacture
- flask
- producing microorganism
- cellulosamaterial
- Prior art date
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims description 22
- 238000004519 manufacturing process Methods 0.000 title claims 2
- 244000005700 microbiome Species 0.000 title claims 2
- 108010059892 Cellulase Proteins 0.000 claims description 6
- 229940106157 cellulase Drugs 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 4
- 241000223261 Trichoderma viride Species 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
- 239000000203 mixture Substances 0.000 description 6
- 239000000706 filtrate Substances 0.000 description 3
- 239000002002 slurry Substances 0.000 description 3
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Description
7603394-3 därifrån, varigenom etanolutbytet ökar. 7603394-3 therefrom, thereby increasing the ethanol yield.
De följande exemplen illustrerar ytterligare processen enligt upp- finningen.The following examples further illustrate the process according to the invention.
Exempel I tvâ skilda skakkolvar odlades Trichoderma viride QM 9414 (ATCC 26 921) aerobt vid 30°C under en period på 6 dygn. Varje kolv inne- höll 100 ml identiskt samma, konventionella näringsmedel med cellu- losapulver som kolkälla. Under odlingen inställdes pH-värdet på 5,4 två gånger dagligen. Vid slutet av sexdagarsperioden filtrerades in- nehållet i ena kolven, A, till ett kulturfiltrat innehållande cel- lulas-enzymkomplexet. Innehållet i den andra kolven, B, filtrerades icke. Båda de sålunda erhållna cellulas-haltiga materialen användes vid processen enligt uppfinningen såsom beskrives nedan. 5 g steriliserat cellulosapulver, 300 mesh, 95 vikt-% cellulosa, sat- sades aseptiskt i var och en av två steriliserade 100 ml kolvar.Example In two separate shake flasks, Trichoderma viride QM 9414 (ATCC 26 921) was grown aerobically at 30 ° C for a period of 6 days. Each flask contained 100 ml of identical identical conventional foodstuffs with cellulose powder as the carbon source. During the cultivation, the pH was adjusted to 5.4 twice daily. At the end of the six day period, the contents of one flask, A, were filtered into a culture filtrate containing the cellulase enzyme complex. The contents of the second flask, B, were not filtered. Both of the cellulase-containing materials thus obtained were used in the process of the invention as described below. 5 g of sterilized cellulose powder, 300 mesh, 95% by weight of cellulose, was aseptically charged into each of two sterilized 100 ml flasks.
Till den ena av dessa kolvar, C, tillsattes 45 ml av det ovannämnda kulturfiltratet från kolv A. Till den andra kolven D tillsattes 45 ml av den ovannämnda, väl omrörda, ofiltrerade kulturuppslamningen från kolv B, dvs hela odlings-vattenuppslamningen utan avskiljning av någon komponent därifrån. Därefter tillsattes till var och en av kolvarna C och D 5 ml av en steriliserad lösning innehållande: asparagin 125 mg KH2PO4 50 mg' MgSO4 . 7H20 150 mg jästextrakt 10 mg destillerat vatten 5 ml Den resulterande blandningen i varje kolv inställdes på pH 4,0 ef- ter behov. Därefter tillsattes två platinaöglor Saccharomyces cerevisiae direkt från en agarplatta till var och en av kolvarna och blandningarna fick reagera anaerobt vid ca 30°C i 96 timmar. När reaktionsblandningarna analyserades hade 1,2 g etanol bildats i blandningen från kolv C, medan 1,4 g etanol fanns i blandningen från kolv D. Sedan reaktionen fått fortsätta ytterligare 96 timmar, innehöll blandningen från kolv C 2,0 g etanol och blandningen från kolv D 2,3 g etanol. 7603394-3 En jämförelse mellan resultaten vid användning av hela kultur- uppslamningen, kolv D, som cellulaskälla och resultaten med använd- ning av odlingsfiltratet, kolv C, som cellulaskälla visar en mar- kant förbättring av det erhållna etanolutbytet, 2,3 g, jämfört med 2,0 g, dvs en ökning med 15%.To one of these flasks, C, was added 45 ml of the above culture filtrate from flask A. To the other flask D was added 45 ml of the above-mentioned, well-stirred, unfiltered culture slurry from flask B, i.e. the whole culture-water slurry without separating any component therefrom. Then 5 ml of a sterilized solution containing: asparagine 125 mg KH 2 PO 4 50 mg 'MgSO 4 was added to each of flasks C and D. 7H 2 O 150 mg yeast extract 10 mg distilled water 5 ml The resulting mixture in each flask was adjusted to pH 4.0 as needed. Then two platinum loops Saccharomyces cerevisiae were added directly from an agar plate to each of the flasks and the mixtures were allowed to react anaerobically at about 30 ° C for 96 hours. When the reaction mixtures were analyzed, 1.2 g of ethanol had formed in the mixture from flask C, while 1.4 g of ethanol was present in the mixture from flask D. After allowing the reaction to proceed for another 96 hours, the mixture from flask C contained 2.0 g of ethanol and the mixture from flask D 2.3 g ethanol. A comparison between the results using the whole culture slurry, flask D, as the cellulase source and the results using the culture filtrate, flask C, as the cellulase source shows a marked improvement in the ethanol yield obtained, 2.3 g. compared to 2.0 g, ie an increase of 15%.
Claims (1)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/610,731 US3990944A (en) | 1974-09-20 | 1975-09-08 | Manufacture of alcohol from cellulosic materials using plural ferments |
| DK422375AA DK140764B (en) | 1974-09-20 | 1975-09-19 | Process for producing alcohol from cellulosic material. |
| DK111576AA DK142880B (en) | 1975-09-08 | 1976-03-15 | Process for preparing alcohol from cellulose material. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SE7603394L SE7603394L (en) | 1977-03-09 |
| SE436756B true SE436756B (en) | 1985-01-21 |
Family
ID=27221119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE7603394A SE436756B (en) | 1975-09-08 | 1976-03-18 | SEE IN THE MANUFACTURE OF ETHANOL FROM CELLULOSAMATERIAL THROUGH THE IMPACT OF CELLULAS AND AN ALCOHOL PRODUCING MICROORGANISM |
Country Status (10)
| Country | Link |
|---|---|
| JP (1) | JPS6043118B2 (en) |
| BE (1) | BE839573R (en) |
| DE (1) | DE2609551C2 (en) |
| DK (1) | DK142880B (en) |
| FI (1) | FI760738A (en) |
| GB (1) | GB1497958A (en) |
| IT (1) | IT1064799B (en) |
| NL (1) | NL7602796A (en) |
| NO (1) | NO145101C (en) |
| SE (1) | SE436756B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009049067A1 (en) * | 2007-10-12 | 2009-04-16 | Danisco Us Inc., Genencor Division | Methods and compositions for enhanced production of organic sustances from fermenting microorganisms |
| CN102325889A (en) * | 2008-12-19 | 2012-01-18 | 诺维信股份有限公司 | Increase the method for cellulose materials hydrolysis |
-
1976
- 1976-03-08 DE DE2609551A patent/DE2609551C2/en not_active Expired
- 1976-03-12 GB GB9894/76A patent/GB1497958A/en not_active Expired
- 1976-03-15 BE BE165169A patent/BE839573R/en active
- 1976-03-15 DK DK111576AA patent/DK142880B/en unknown
- 1976-03-17 NL NL7602796A patent/NL7602796A/en not_active Application Discontinuation
- 1976-03-17 IT IT21315/76A patent/IT1064799B/en active
- 1976-03-18 SE SE7603394A patent/SE436756B/en not_active IP Right Cessation
- 1976-03-18 JP JP51029748A patent/JPS6043118B2/en not_active Expired
- 1976-03-19 NO NO760977A patent/NO145101C/en unknown
- 1976-03-19 FI FI760738A patent/FI760738A/fi not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| DK142880B (en) | 1981-02-16 |
| DK142880C (en) | 1981-08-31 |
| JPS6043118B2 (en) | 1985-09-26 |
| GB1497958A (en) | 1978-01-12 |
| NO145101B (en) | 1981-10-05 |
| JPS5234981A (en) | 1977-03-17 |
| DE2609551C2 (en) | 1982-06-03 |
| NO760977L (en) | 1977-03-09 |
| DE2609551A1 (en) | 1977-03-10 |
| SE7603394L (en) | 1977-03-09 |
| DK111576A (en) | 1977-03-09 |
| FI760738A (en) | 1977-03-06 |
| IT1064799B (en) | 1985-02-25 |
| NO145101C (en) | 1982-01-13 |
| NL7602796A (en) | 1977-03-10 |
| BE839573R (en) | 1976-09-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| NUG | Patent has lapsed |
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