SU499868A1 - Method of cultivating bacterial entomopathogenic microorganisms - Google Patents
Method of cultivating bacterial entomopathogenic microorganismsInfo
- Publication number
- SU499868A1 SU499868A1 SU2043102A SU2043102A SU499868A1 SU 499868 A1 SU499868 A1 SU 499868A1 SU 2043102 A SU2043102 A SU 2043102A SU 2043102 A SU2043102 A SU 2043102A SU 499868 A1 SU499868 A1 SU 499868A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- microorganisms
- cultivating bacterial
- entomopathogenic microorganisms
- entomopathogenic
- bacterial
- Prior art date
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
Изобретение относитс к микробиологической промышленности и может быть испо зовано при производстве энеомопатогенных бактериальных препаратов, в основе которых лежат микррорганизмы группы Sacitias thuringiensis. Известен способ получени бактериального препарата, при котором культивирование микроорганизмов ведут в глубинных услови х на жидкой питательной среде, где источником азота служит кукурузный экст ракт или кормовые дрожжи, а источником углеводов - глюкоза или другие продукты, содержащие углеводы. После окончани про цесса микробна биомасса отдел етс на сепараторах, а недосадочна жидкость (фугат ) сливаетс в канализацию, в результате чего ингредиенты питательной среды используютс неполностью и выливаютс с фугатов, вл сь отходами производства, при этом образуетс большое количество промышленных стоков (на 1 т препарата 49 т стоков), 11ель изобретени - более полное исполь зование ингредиентов питательной среды и ликвидаци промышленных стоков. Поставленна цель достигаетс тем, что культивирование бактериальных энтомопатогенных микроорганизмов группы fidc, fturwjiertSis ведут по замкнутому циклу, т. е. на фуГате, полученном после выращивани микроорганизма по-известному способу , выращивают дрожжи рода Caudlda или Clostridlu-m pasterianttm , d на получен ной суспензии микроорганизмов вновь выращивают исходные культуры. Пример. После выращивани энто- мопатогенных микроорганизмов описанным выше епособом и отделени биомассы на сепараторах полученный фугат собирают в емкость необходимого объема и стерилизуют паром обычным способом (1 атм в течение 1-1,5 ч). Если фугат содержит не менее 40 мг/% аминного азота, то к нему добавл ют О,5-« -1% углеводов (глюкозы, крахмала, кукурузной муки и т. п.) и вновь стерилизуют. Если фугат содержит менее 4О мг/% аминного азота, то содержание аминногоThe invention relates to the microbiological industry and can be used in the production of pathogenic bacterial preparations based on microorganisms of the Sacitias thuringiensis group. A known method for producing a bacterial preparation in which the cultivation of microorganisms is carried out in deep conditions on a liquid nutrient medium, where corn source or corn yeast or fodder yeast is used as a source of nitrogen, and glucose or other products containing carbohydrates is a source of carbohydrates. At the end of the process, the microbial biomass is separated at the separators, and the undersized liquid (the fugate) is drained to the sewage system, as a result of which the nutrient medium ingredients are used incompletely and discharged from the fugates to be a waste, producing a large amount of industrial effluent (per ton 49 tons of wastewater), 11el of the invention - a more complete use of nutrient medium ingredients and the elimination of industrial effluents. This aim is achieved in that the culturing bacterial entomopathogenic microorganisms fidc group, fturwjiertSis are in a closed cycle, that is. E. On the centrate obtained after growth of a microorganism in the known method, cultured yeast of genus Caudlda or Clostridlu-m pasterianttm, d on the obtained hydrochloric suspension microorganisms re-grow the original culture. Example. After the growth of entomopathogenic microorganisms by the method described above and the separation of biomass on separators, the resulting centrate is collected in a container of the required volume and sterilized by steam in the usual way (1 atm for 1-1.5 hours). If the centrate contains at least 40 mg /% amino nitrogen, O, 5- > -1% carbohydrates (glucose, starch, cornmeal, etc.) are added to it and sterilized again. If the centrate contains less than 4O mg /% amino nitrogen, the amine content
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU2043102A SU499868A1 (en) | 1974-07-09 | 1974-07-09 | Method of cultivating bacterial entomopathogenic microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU2043102A SU499868A1 (en) | 1974-07-09 | 1974-07-09 | Method of cultivating bacterial entomopathogenic microorganisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU499868A1 true SU499868A1 (en) | 1976-01-25 |
Family
ID=20590706
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU2043102A SU499868A1 (en) | 1974-07-09 | 1974-07-09 | Method of cultivating bacterial entomopathogenic microorganisms |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU499868A1 (en) |
-
1974
- 1974-07-09 SU SU2043102A patent/SU499868A1/en active
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