DK1056884T3 - Fremgangsmåde til ikke-specifik amplifikation af nukleinsyre - Google Patents

Fremgangsmåde til ikke-specifik amplifikation af nukleinsyre

Info

Publication number
DK1056884T3
DK1056884T3 DK99904801T DK99904801T DK1056884T3 DK 1056884 T3 DK1056884 T3 DK 1056884T3 DK 99904801 T DK99904801 T DK 99904801T DK 99904801 T DK99904801 T DK 99904801T DK 1056884 T3 DK1056884 T3 DK 1056884T3
Authority
DK
Denmark
Prior art keywords
rna
sequence
enzyme
mrna
rna polymerase
Prior art date
Application number
DK99904801T
Other languages
Danish (da)
English (en)
Inventor
Gemen B Van
Original Assignee
Pamgene Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pamgene Bv filed Critical Pamgene Bv
Application granted granted Critical
Publication of DK1056884T3 publication Critical patent/DK1056884T3/da

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6865Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
DK99904801T 1998-02-27 1999-01-21 Fremgangsmåde til ikke-specifik amplifikation af nukleinsyre DK1056884T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98200619 1998-02-27
PCT/EP1999/000332 WO1999043850A1 (en) 1998-02-27 1999-01-21 Method for the non-specific amplification of nucleic acid

Publications (1)

Publication Number Publication Date
DK1056884T3 true DK1056884T3 (da) 2002-02-25

Family

ID=8233425

Family Applications (1)

Application Number Title Priority Date Filing Date
DK99904801T DK1056884T3 (da) 1998-02-27 1999-01-21 Fremgangsmåde til ikke-specifik amplifikation af nukleinsyre

Country Status (14)

Country Link
US (3) US6338954B1 (de)
EP (2) EP1056884B9 (de)
JP (1) JP3641206B2 (de)
KR (1) KR100440044B1 (de)
AT (1) ATE210736T1 (de)
AU (1) AU748847B2 (de)
CA (1) CA2322073C (de)
DE (1) DE69900592T2 (de)
DK (1) DK1056884T3 (de)
ES (1) ES2169943T3 (de)
ID (1) ID26107A (de)
PT (1) PT1056884E (de)
WO (1) WO1999043850A1 (de)
ZA (1) ZA99617B (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6582938B1 (en) * 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
US6794138B1 (en) * 1999-12-16 2004-09-21 Affymetrix, Inc. Methods of small sample amplification
AU2001254670A1 (en) * 2000-03-02 2001-09-12 Akzo Nobel N.V. Detection of hepatitis b virus rna
US9261460B2 (en) * 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US7727722B2 (en) * 2001-08-29 2010-06-01 General Electric Company Ligation amplification
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
JP2006504440A (ja) * 2002-10-30 2006-02-09 パムジーン ベー.ベー. 複数のrnaコピーを作成するための改良方法
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US6852494B2 (en) 2003-01-10 2005-02-08 Linden Technologies, Inc. Nucleic acid amplification
DE602004022282D1 (de) 2003-05-09 2009-09-10 Genisphere Inc Verfahren zur amplifikation von nukleinsäuresequenzen mittels gestaffelter ligation
US20050153333A1 (en) * 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
WO2005098044A1 (en) * 2004-04-01 2005-10-20 Genisphere Inc. Methods for amplification of nucleic acid sequences using promoter templates
FR2876116B1 (fr) * 2004-10-01 2007-04-06 Biomerieux Sa Methode pour generer des transcrits
EP1828422A4 (de) * 2004-11-15 2008-07-30 Bacterial Barcodes Inc Detektion mithilfe von primern zu wiederholter dna und transkriptionsbasierte amplifikation dadurch
EP1836302B1 (de) * 2004-12-23 2010-04-14 Amersham Biosciences Corp. Rna-amplifikation auf ligationsbasis
AU2012200749B2 (en) * 2004-12-23 2012-10-04 Global Life Sciences Solutions Usa Llc Ligation-based RNA amplification
EP1863908B1 (de) 2005-04-01 2010-11-17 Qiagen GmbH Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
DE102006038113A1 (de) * 2006-08-14 2008-02-21 Qiagen Gmbh Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität
JP5409631B2 (ja) * 2007-08-13 2014-02-05 キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング 酵素反応においてサンプル中でcDNAを合成するための方法
WO2009052214A2 (en) * 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
WO2009158119A2 (en) 2008-05-30 2009-12-30 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of salmonella
EP2280081A1 (de) * 2009-07-31 2011-02-02 Qiagen GmbH Verfahren zur standardisierten Quantifizierung von RNA
EP2280080A1 (de) 2009-07-31 2011-02-02 Qiagen GmbH Verfahren zur standardisierten Quantifizierung von Nukleinsäuren unter Verwendung von Anker-Oligonukleotiden und Adapter-Oligonukleotiden

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4661450A (en) * 1983-05-03 1987-04-28 Molecular Genetics Research And Development Limited Partnership Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides
US5472850A (en) * 1991-04-10 1995-12-05 Oklahoma Medical Research Foundation Quantitative clotting assay for activated factor VII
DE69034177T2 (de) * 1989-07-11 2005-10-27 Gen-Probe Inc., San Diego Verfahren zur Amplifikation von Nukleinsäuresequenzen
US5166057A (en) 1989-08-28 1992-11-24 The Mount Sinai School Of Medicine Of The City University Of New York Recombinant negative strand rna virus expression-systems
US5545522A (en) * 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US5169766A (en) * 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
AU681082B2 (en) * 1992-05-06 1997-08-21 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
US5514545A (en) 1992-06-11 1996-05-07 Trustees Of The University Of Pennsylvania Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
ATE209258T1 (de) * 1994-07-15 2001-12-15 Akzo Nobel Nv Verwendung von rna-polymerase zur verbesserung von nukeinsaeure-amplifikationsverfahren
FR2724934B1 (fr) * 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
JP2002515738A (ja) * 1996-01-23 2002-05-28 アフィメトリックス,インコーポレイティド 核酸分析法
US6114152A (en) * 1997-12-12 2000-09-05 The Regents Of The University Of California Methods for making nucleic acids

Also Published As

Publication number Publication date
US20020061533A1 (en) 2002-05-23
AU748847B2 (en) 2002-06-13
ATE210736T1 (de) 2001-12-15
WO1999043850A1 (en) 1999-09-02
EP1170381A2 (de) 2002-01-09
DE69900592D1 (de) 2002-01-24
AU2518999A (en) 1999-09-15
EP1056884B9 (de) 2002-07-24
US20030113875A1 (en) 2003-06-19
EP1056884B1 (de) 2001-12-12
KR100440044B1 (ko) 2004-07-14
US6338954B1 (en) 2002-01-15
EP1056884A1 (de) 2000-12-06
CA2322073C (en) 2003-12-23
ES2169943T3 (es) 2002-07-16
JP3641206B2 (ja) 2005-04-20
DE69900592T2 (de) 2002-08-22
KR20010041371A (ko) 2001-05-15
ZA99617B (en) 1999-07-27
EP1170381A3 (de) 2004-01-28
ID26107A (id) 2000-11-23
JP2002505087A (ja) 2002-02-19
PT1056884E (pt) 2002-06-28
CA2322073A1 (en) 1999-09-02

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