DK0894091T3 - Fremgangsmåde til oprensning af et protein fra en proteinholdig opløsning - Google Patents

Fremgangsmåde til oprensning af et protein fra en proteinholdig opløsning

Info

Publication number
DK0894091T3
DK0894091T3 DK97914167T DK97914167T DK0894091T3 DK 0894091 T3 DK0894091 T3 DK 0894091T3 DK 97914167 T DK97914167 T DK 97914167T DK 97914167 T DK97914167 T DK 97914167T DK 0894091 T3 DK0894091 T3 DK 0894091T3
Authority
DK
Denmark
Prior art keywords
purifying
protein
proteinaceous solution
cellulase
crystalline form
Prior art date
Application number
DK97914167T
Other languages
Danish (da)
English (en)
Inventor
Anders Rancke-Madsen
Mads Aage Laustsen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK0894091T3 publication Critical patent/DK0894091T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/30Extraction; Separation; Purification by precipitation
    • C07K1/306Extraction; Separation; Purification by precipitation by crystallization
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/814Enzyme separation or purification
    • Y10S435/816Enzyme separation or purification by solubility

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
DK97914167T 1996-03-15 1997-03-14 Fremgangsmåde til oprensning af et protein fra en proteinholdig opløsning DK0894091T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK30596 1996-03-15
PCT/DK1997/000113 WO1997034919A1 (en) 1996-03-15 1997-03-14 Method for purification of a protein from a protein containing solution

Publications (1)

Publication Number Publication Date
DK0894091T3 true DK0894091T3 (da) 2003-06-10

Family

ID=8092063

Family Applications (1)

Application Number Title Priority Date Filing Date
DK97914167T DK0894091T3 (da) 1996-03-15 1997-03-14 Fremgangsmåde til oprensning af et protein fra en proteinholdig opløsning

Country Status (10)

Country Link
US (1) US6087148A (es)
EP (1) EP0894091B1 (es)
JP (1) JP2000506534A (es)
CN (1) CN1216899C (es)
AT (1) ATE233784T1 (es)
AU (1) AU2151997A (es)
DE (1) DE69719497T2 (es)
DK (1) DK0894091T3 (es)
IN (1) IN190120B (es)
WO (1) WO1997034919A1 (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69719497T2 (de) * 1996-03-15 2003-10-30 Novozymes A/S, Bagsvaerd Verfahren zur proteinaufreinigung aus einer proteinhaltigen lösung
SG121739A1 (en) * 1998-04-27 2006-05-26 Altus Pharmaceuticals Inc Stabilized protein crystals, formulations containing them and methods of making them
DE19859876A1 (de) * 1998-12-23 2000-06-29 Fraunhofer Ges Forschung Verfahren zur Kristallisation aus Lösungen
GB9910975D0 (en) 1999-05-13 1999-07-14 Univ Strathclyde Rapid dehydration of proteins
US6663602B2 (en) 2000-06-16 2003-12-16 Novo Nordisk A/S Injection device
EP1447409B1 (en) * 2001-10-25 2009-03-11 Kyowa Hakko Kogyo Co., Ltd. Crystal of oxidized glutathione and process for producing the same
DE60326012D1 (de) * 2002-11-01 2009-03-12 Bayer Healthcare Llc Verfahren zur Konzentration von Proteinen
AU2005298944B2 (en) 2004-10-21 2010-12-23 Novo Nordisk A/S Dial-down mechanism for wind-up pen
WO2007113188A1 (en) * 2006-03-30 2007-10-11 Novo Nordisk A/S Two-phase precipitation of proteins
DE602007004972D1 (de) 2006-05-16 2010-04-08 Novo Nordisk As Getriebemechanismus für ein injektionsgerät
US9192727B2 (en) 2006-05-18 2015-11-24 Novo Nordisk A/S Injection device with mode locking means
DE102008029401A1 (de) * 2008-06-23 2009-12-24 Bayer Technology Services Gmbh Verfahren zur Kristallisation von Peptiden und Proteinen
FR2943686B1 (fr) * 2009-03-30 2013-11-01 Roquette Freres Procede d'obtention d'une preparation de beta-amylases a partir des fractions solubles de plantes amidonnieres
RU2014128518A (ru) 2011-12-29 2016-02-20 Ново Нордиск А/С Механизм увеличения/уменьшения дозы для взводимого шприца-ручки
WO2023200284A1 (ko) * 2022-04-15 2023-10-19 재단법인대구경북과학기술원 생체 시료 내 지질 연관 단백질의 질량 분석을 위한 지질 연관 단백질의 정제 및 농축 방법
WO2024032881A1 (en) * 2022-08-10 2024-02-15 Arla Foods Amba Crystallisation of a protein capable of crystallizing under salting-in conditions using multiple protein feeds

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3664926A (en) * 1970-02-26 1972-05-23 Merck & Co Inc Crystallization of l-asparaginase
DE4001238A1 (de) * 1990-01-18 1991-07-25 Basf Ag Verfahren zur anreicherung bzw. reinigung von biomolekuelen
DK115890D0 (da) * 1990-05-09 1990-05-09 Novo Nordisk As Enzym
DK83093D0 (da) * 1993-07-09 1993-07-09 Novo Nordisk As Fremgangsmaade
DE69719497T2 (de) * 1996-03-15 2003-10-30 Novozymes A/S, Bagsvaerd Verfahren zur proteinaufreinigung aus einer proteinhaltigen lösung

Also Published As

Publication number Publication date
IN190120B (es) 2003-06-14
US6087148A (en) 2000-07-11
WO1997034919A1 (en) 1997-09-25
AU2151997A (en) 1997-10-10
EP0894091A1 (en) 1999-02-03
DE69719497D1 (de) 2003-04-10
ATE233784T1 (de) 2003-03-15
JP2000506534A (ja) 2000-05-30
CN1216899C (zh) 2005-08-31
CN1213377A (zh) 1999-04-07
EP0894091B1 (en) 2003-03-05
DE69719497T2 (de) 2003-10-30

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