DK0497828T4 - Fremgangsmåde til endoproteolytisk behandling af (forstadie)proteiner og til (mikro)biologisk produktion af proteiner. - Google Patents

Fremgangsmåde til endoproteolytisk behandling af (forstadie)proteiner og til (mikro)biologisk produktion af proteiner.

Info

Publication number
DK0497828T4
DK0497828T4 DK90915837T DK90915837T DK0497828T4 DK 0497828 T4 DK0497828 T4 DK 0497828T4 DK 90915837 T DK90915837 T DK 90915837T DK 90915837 T DK90915837 T DK 90915837T DK 0497828 T4 DK0497828 T4 DK 0497828T4
Authority
DK
Denmark
Prior art keywords
furin
proteins
protein
micro
biological production
Prior art date
Application number
DK90915837T
Other languages
Danish (da)
English (en)
Other versions
DK0497828T3 (da
Inventor
De Ven Willem Jan Marie Van
Den Ouweland Anna Maria Wi Van
Duijnhoven Johannes Lamber Van
Antonius Johannes Mar Roebroek
Piet Nico Maria Koning
Original Assignee
Univ Leuven Kath
De Ven Willem Jan Marie Van
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26646599&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK0497828(T4) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from NL8902651A external-priority patent/NL8902651A/nl
Application filed by Univ Leuven Kath, De Ven Willem Jan Marie Van filed Critical Univ Leuven Kath
Application granted granted Critical
Publication of DK0497828T3 publication Critical patent/DK0497828T3/da
Publication of DK0497828T4 publication Critical patent/DK0497828T4/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6454—Dibasic site splicing serine proteases, e.g. kexin (3.4.21.61); furin (3.4.21.75) and other proprotein convertases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/482—Serine endopeptidases (3.4.21)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/82—Translation products from oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/32—Fusion polypeptide fusions with soluble part of a cell surface receptor, "decoy receptors"
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Oncology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Plant Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK90915837T 1989-10-25 1990-10-12 Fremgangsmåde til endoproteolytisk behandling af (forstadie)proteiner og til (mikro)biologisk produktion af proteiner. DK0497828T4 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
NL8902651A NL8902651A (nl) 1989-10-25 1989-10-25 Farmaceutisch preparaat met endoproteolytische activiteit; werkwijze voor endoproteolytische processing van eiwitten.
NL9000917A NL9000917A (nl) 1989-10-25 1990-04-18 Farmaceutisch preparaat met endoproteolytische activiteit; werkwijze voor endoproteolytische processing van (precursor) eiwitten en voor de (micro) biologische bereiding van eiwitten.
PCT/NL1990/000151 WO1991006314A2 (en) 1989-10-25 1990-10-12 Pharmaceutical composition having an endoproteolytic activity; a process for endoproteolytically processing (precursor) proteins and for the (micro)biological production of proteins

Publications (2)

Publication Number Publication Date
DK0497828T3 DK0497828T3 (da) 1997-07-07
DK0497828T4 true DK0497828T4 (da) 2008-02-04

Family

ID=26646599

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90915837T DK0497828T4 (da) 1989-10-25 1990-10-12 Fremgangsmåde til endoproteolytisk behandling af (forstadie)proteiner og til (mikro)biologisk produktion af proteiner.

Country Status (13)

Country Link
US (1) US5989856A (de)
EP (3) EP0885956A1 (de)
JP (2) JP3191111B2 (de)
AT (1) ATE147437T1 (de)
AU (1) AU6613290A (de)
CA (1) CA2069929C (de)
DE (1) DE69029663T3 (de)
DK (1) DK0497828T4 (de)
ES (1) ES2099714T5 (de)
FI (1) FI105348B (de)
GR (1) GR3023013T3 (de)
NL (1) NL9000917A (de)
WO (1) WO1991006314A2 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5935815A (en) * 1989-10-25 1999-08-10 Katholieke Universiteit Leuven Process for micro biological production of proteins
JP4236698B2 (ja) * 1990-11-26 2009-03-11 ジェネティックス インスティチュート，リミテッド ライアビリティ カンパニー 宿主細胞でのｐａｃｅの発現およびその使用法
US6348327B1 (en) 1991-12-06 2002-02-19 Genentech, Inc. Non-endocrine animal host cells capable of expressing variant proinsulin and processing the same to form active, mature insulin and methods of culturing such cells
DK52293D0 (de) * 1993-05-05 1993-05-05 Novo Nordisk As
US6005077A (en) * 1995-11-10 1999-12-21 Immuno Aktiengesellschaft Use of von willebrand factor and pharmaceutical formulation
AT404838B (de) * 1995-11-24 1999-03-25 Immuno Ag Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
US6596526B1 (en) 2000-06-09 2003-07-22 Baxter Aktiengesellschaft Furin polypeptides with improved characteristics
WO2005103245A1 (en) * 2004-04-23 2005-11-03 Novozymes A/S Method for obtaining mature protease by enzymatic digestion
US8343748B2 (en) * 2007-05-22 2013-01-01 Baxter International Inc. Preparative purification process for human furin
KR101818411B1 (ko) 2007-12-27 2018-01-12 박스알타 인코퍼레이티드 세포 배양 방법
DK2240578T3 (da) 2007-12-31 2019-06-17 Baxalta GmbH I alt væsentligt dyreproteinfrit rekombinant furin og fremgangsmåder til fremstilling af samme
US20130149295A1 (en) 2010-05-12 2013-06-13 Martine Cohen-Solal Furin and biologically active derivatives thereof for use in the prevention or treatment of an inflammatory disease
BR112013030880B1 (pt) 2011-06-02 2022-04-12 Takeda Pharmaceutical Company Limited Composição aquosa estabilizada de furina recombinante

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0246709A1 (de) * 1986-05-20 1987-11-25 Stichting Katholieke Universiteit Rekombinantes DNS, komplementäre DNS, Protein, Antikörper und Verfahren zum Nachweis von Tumorzellen
CA1340740C (en) * 1987-12-08 1999-09-14 Eileen R. Mulvihill Co-expression in eukaryotic cells

Also Published As

Publication number Publication date
EP0497828A1 (de) 1992-08-12
ATE147437T1 (de) 1997-01-15
GR3023013T3 (en) 1997-07-30
JP3270760B2 (ja) 2002-04-02
AU6613290A (en) 1991-05-31
FI921847A0 (fi) 1992-04-24
EP0497828B1 (de) 1997-01-08
DK0497828T3 (da) 1997-07-07
FI921847A (fi) 1992-04-24
NL9000917A (nl) 1991-05-16
WO1991006314A2 (en) 1991-05-16
FI105348B (fi) 2000-07-31
CA2069929A1 (en) 1991-04-26
ES2099714T3 (es) 1997-06-01
DE69029663T3 (de) 2008-05-21
CA2069929C (en) 2002-08-27
JP3191111B2 (ja) 2001-07-23
EP0693286A1 (de) 1996-01-24
ES2099714T5 (es) 2008-04-01
EP0497828B2 (de) 2007-10-03
JP2001238683A (ja) 2001-09-04
US5989856A (en) 1999-11-23
EP0885956A1 (de) 1998-12-23
JPH05504051A (ja) 1993-07-01
DE69029663T2 (de) 1997-09-04
WO1991006314A3 (en) 1991-09-05
DE69029663D1 (de) 1997-02-20

Similar Documents

Publication Publication Date Title
DK0497828T4 (da) Fremgangsmåde til endoproteolytisk behandling af (forstadie)proteiner og til (mikro)biologisk produktion af proteiner.
NZ510358A (en) Method for recovering proteins from the interstitial fluid of plant tissues
DK0611157T3 (da) Fremgangsmåde til fremstilling af nukleinsyrer fra en biologisk prøve ved anvendelse af lavt pH og sur protease
DK0574402T3 (da) Ekspression af PACE i værtsceller og fremgangsmåder til anvendelse deraf
DK1294910T3 (da) Protein glykosyleringsmodifikation i Pichia Pastoris
DK0389063T4 (da) Fremgangsmåde til isolering af nukleinsyre
ATE195148T1 (de) Prozess zur rekombinanten herstellung von hiv-2 - proteinen sowie zellen, die hiv-2 - proteine exprimiert
IL68366A (en) Process for expressing a gene encoding a protein comprising the enzymatic portion of human urokinase in a microorganism or cell culture
KR970009949B1 (en) Novel serine proteolytic enzyme and its process
AU670571B2 (en) A method of proteolytically cleaving proteins and the use of said method
DE69031042D1 (de) Fermentationsprozess zur herstellung von pyrimidin
DE58905691D1 (de) Verfahren zur Herstellung von L-alpha-Aminosäuren.
DE69939876D1 (de) Methoden zur massenkultivierung von zellen die rekombinantes menschliches erythropoietin produzieren
EP0188312A3 (de) Verfahren zur Herstellung eines Proteins
IL104003A0 (en) Recombinant vaccines and method of producing same
PT592574E (pt) Processo de producao de proteinas recombinantes e celulas hospedeiras utilizadas
GB1382473A (en) Enzyme preparation