DE726888C - Process for the preparation of solutions or suspensions of the blood sugar lowering hormone of the pancreatic gland - Google Patents

Process for the preparation of solutions or suspensions of the blood sugar lowering hormone of the pancreatic gland

Info

Publication number
DE726888C
DE726888C DEI63490D DEI0063490D DE726888C DE 726888 C DE726888 C DE 726888C DE I63490 D DEI63490 D DE I63490D DE I0063490 D DEI0063490 D DE I0063490D DE 726888 C DE726888 C DE 726888C
Authority
DE
Germany
Prior art keywords
solutions
suspensions
hormone
blood sugar
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DEI63490D
Other languages
German (de)
Inventor
Eugen Doerzbach
Dr Carl Ludwig Lautenschlaeger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens AG
Original Assignee
Siemens AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Siemens AG filed Critical Siemens AG
Priority to DEI63490D priority Critical patent/DE726888C/en
Application granted granted Critical
Publication of DE726888C publication Critical patent/DE726888C/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C07K14/625—Extraction from natural sources

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Medicinal Preparation (AREA)
  • Infusion, Injection, And Reservoir Apparatuses (AREA)

Description

Verfahren. zur Herstellung von.Lösungen oder Suspensionen des blutzuckersenkenden Hormons der Pankreasdrüse In dem Patent 725 439 ist ein Verfahren zur Herstellung von haltbaren neutralen Lösungen des blutzuckersernkenden Hormons der Pankreasdrüse beschrieben, welches dadurch gekennzeichnet ist, daß man wäßrige Lösungen des Hormons in Gegenwart von Polyvinylalkohol auf den Neutralpunkt einstellt. Es ist dabei darauf hingewiesen worden, daß neutrale wäßrige Lösungen des Inselhormons nicht beständig sind, sondern innerhalb verhältnismäßig kurzer Zeit ausflocken. Dieser Nachteil läßt sich, wie dargelegt, durch die Mitverwendung von Polyvinylalkohol in -der Lösung vermeiden, Außerdem zeigen die mit Hilfe von Polyvinylalkohol hergestellten Lösungen nach der Injektion eine länger dauernde Wirkung als die bisher bekannten sauren Lösungen.Procedure. for the preparation of.solutions or suspensions of the blood sugar lowering Pancreatic hormone In patent 725,439 is a method of manufacture of durable neutral solutions of the blood sugar-reducing hormone of the pancreatic gland described, which is characterized in that one aqueous solutions of the hormone adjusts to neutral point in the presence of polyvinyl alcohol. It is on it it has been pointed out that neutral aqueous solutions of the islet hormone are not stable but flocculate within a relatively short time. This disadvantage can, as explained, by using polyvinyl alcohol in the solution avoid, also show the solutions made with the help of polyvinyl alcohol after the injection a longer lasting effect than the previously known acidic ones Solutions.

In weiterer Ausgestaltung dieser Erfindung wurde nun gefunden, daß man zu Hormonpräparaten von verlängerter Wirkung auch dann gelangen kann, wenn man wäßrige Lösungen des Inselhormons, die einen Zusatz von Polyvinylalkohol enthalten, auf einen zwischen 4,5 und dem Neutralpunkt liegenden pg-Wert einstellt. Wenn es in diesem Falle im Gegensatz zu dem in dem Patent 725 439 beschriebenen Verfahren beim Unterschreiten einer bestimmten pH-Grenze auch nicht mehr gelingt, das Hormon in Lösung zu halten, so führt doch der Zusatz von Polyvinylalkohol, abgesehen von der erwähnten protrahierten Wirkung, zu einer Ausfällung, die wesentlich feiner verteilt ist als eine ohne den Zusatz der genannten Substanz erhaltene Ausfällung. Dies wirkt sich insbesondere bei der Injektion vorteilhaft aus, da es naturgemäß wesentlich leichter und angenehmer ist, eine feindisperse Suspension zu injizieren als eine grobdisperse.In a further embodiment of this invention it has now been found that you can get to hormone preparations with a prolonged effect even if you aqueous solutions of the island hormone, which contain an addition of polyvinyl alcohol, set to a pg value between 4.5 and the neutral point. If it in this case in contrast to the method described in patent 725,439 when falling below a certain pH limit no longer succeeds in keeping the hormone in solution, the addition of polyvinyl alcohol leads to apart from the aforementioned protracted effect, a precipitation which is essential is more finely divided than a precipitate obtained without the addition of the substance mentioned. This has a particularly advantageous effect during the injection, since it is natural it is much easier and more pleasant to inject a finely dispersed suspension as a coarsely dispersed.

Beispiel 420 mg kristallisiertes Inselhormon werden in io ccm n/io-Salzsäure gelöst und mit 390 ccm einer 5 °/oigen Lösung von Polyvinylalkohol verdünnt. In diese Mischung, die auf 40° C erhitzt ist, und in die eine Glaselektrode in der üblichen Anordnung zur Messung der Wasserstoffionenkonzentration taucht, läßt man unter gutem Umrühren in dünnem Strahle so lange eine o,2°/oige Lösung von Natriumcarbonat zufließen, bis der pH-Wert = 5,5 ist. Darauf kühlt man unter dauerndem Umrühren auf Zimmertemperatur ab. Das Produkt wird nunmehr mit Wasser auf 500 ccm aufgefüllt.Example 420 mg of crystallized islet hormone are dissolved in 10 cc of normal hydrochloric acid and diluted with 390 cc of a 5% solution of polyvinyl alcohol. In this mixture, which is heated to 40 ° C., and into which a glass electrode is immersed in the usual arrangement for measuring the hydrogen ion concentration, a 0.2% solution of sodium carbonate is allowed to flow in a thin stream with thorough stirring until the pH = 5.5. It is then cooled to room temperature with constant stirring. The product is now made up to 500 cc with water.

An Stelle der Natriumcarbonatlösung kann man Natronlauge oder Natriumphosphatlösung verwenden.Instead of the sodium carbonate solution, caustic soda or sodium phosphate solution can be used use.

Claims (1)

PATENTANSPRUCH: Verfahren zur Herstellung von Lösungen bzw. Suspensionen des blutzuckersenkenden Hormons der Pankreasdrüse nach Patent 725 439, dadurch gekennzeichnet, daß man wäßrige Hormonlösungen in Gegenwart von Polyvinylalkohol auf einen zwischen 4,5 und dem LTeutralpunkt liegenden PH-Wert einstellt.PATENT CLAIM: Process for the production of solutions or suspensions of the blood sugar lowering hormone of the pancreatic gland according to Patent 725 439, characterized in that that one aqueous hormone solutions in the presence of polyvinyl alcohol to an between 4.5 and the L neutral point pH adjusts.
DEI63490D 1939-01-14 1939-01-14 Process for the preparation of solutions or suspensions of the blood sugar lowering hormone of the pancreatic gland Expired DE726888C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DEI63490D DE726888C (en) 1939-01-14 1939-01-14 Process for the preparation of solutions or suspensions of the blood sugar lowering hormone of the pancreatic gland

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEI63490D DE726888C (en) 1939-01-14 1939-01-14 Process for the preparation of solutions or suspensions of the blood sugar lowering hormone of the pancreatic gland

Publications (1)

Publication Number Publication Date
DE726888C true DE726888C (en) 1942-10-22

Family

ID=7195913

Family Applications (1)

Application Number Title Priority Date Filing Date
DEI63490D Expired DE726888C (en) 1939-01-14 1939-01-14 Process for the preparation of solutions or suspensions of the blood sugar lowering hormone of the pancreatic gland

Country Status (1)

Country Link
DE (1) DE726888C (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3443877A1 (en) * 1984-06-09 1985-12-12 Hoechst Ag Insulin preparations, process for their preparation, and their use

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3443877A1 (en) * 1984-06-09 1985-12-12 Hoechst Ag Insulin preparations, process for their preparation, and their use

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