DE700113C - Process for the production of stable, sterilizable emulsions of clot-activating phosphatides - Google Patents

Process for the production of stable, sterilizable emulsions of clot-activating phosphatides

Info

Publication number
DE700113C
DE700113C DE1939G0100246 DEG0100246D DE700113C DE 700113 C DE700113 C DE 700113C DE 1939G0100246 DE1939G0100246 DE 1939G0100246 DE G0100246 D DEG0100246 D DE G0100246D DE 700113 C DE700113 C DE 700113C
Authority
DE
Germany
Prior art keywords
emulsions
stable
sterilizable
phosphatides
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE1939G0100246
Other languages
German (de)
Inventor
Dr Jean Druey
Dr Max Hartmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chemische Ind Ges
GESELLSCHAFT fur CHEMISCHE INDUSTRIE
BASF Schweiz AG
Original Assignee
Chemische Ind Ges
GESELLSCHAFT fur CHEMISCHE INDUSTRIE
Gesellschaft fuer Chemische Industrie in Basel CIBA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chemische Ind Ges, GESELLSCHAFT fur CHEMISCHE INDUSTRIE, Gesellschaft fuer Chemische Industrie in Basel CIBA filed Critical Chemische Ind Ges
Application granted granted Critical
Publication of DE700113C publication Critical patent/DE700113C/en
Expired legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/16—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing nitrogen, e.g. nitro-, nitroso-, azo-compounds, nitriles, cyanates
    • A61K47/18—Amines; Amides; Ureas; Quaternary ammonium compounds; Amino acids; Oligopeptides having up to five amino acids
    • A61K47/183—Amino acids, e.g. glycine, EDTA or aspartame

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Epidemiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Description

Verfahren zur Herstellung stabiler sterilisierbarer Emulsionen von gerinnungsaktiven tierischen Phosphatiden Bei der an sich bekannten Aufarbleitung von tierischem Blut auf gerinnungsaktive Phosphatide erhält man bei weitgehender Reinigung Produkte, die sich in den üblichen isotonischen Lösungen nicht sterilisieren lassen; so entsteht beim Erwärmen z. B. mit physiologischer Kochsalz-,Tyrode- oderRingerlösung eine Ausflockung.Process for the preparation of stable, sterilizable emulsions of coagulation-active animal phosphatides with the known Aufarbleitung from animal blood to coagulant phosphatides are obtained by far Cleaning Products that do not sterilize in common isotonic solutions permit; so when heating z. B. with physiological saline, Tyrode or Ringer's solution a flocculation.

Es wurde nun gefunden, daß man zu stabilen sterilisierbaren Emulsionen gelangen kann, wenn man Emulsionen von gerinnungsaktiven Phosphatiden Aminosäuren zusetzt. Als Aminosäuren verwendet man ganz allgemein leicht lösliche Aminosäuren oder auch Gemische von solchen, insbesondere Glykokoll. Die Konzentration dieser zugefügten Aminosäuren wird zwecksmäßig so gewählt, daß eine blutisotonische Lösung erhalten wird. Die gewonnenen Emulsionen lassen sich ohne Veränderung sterilisieren. Es tritt keine Ausflockung ein. Die Wasserstoffionenkonzentration bleibt vor und nach der Sterilisation dieselbe. Die am Hühnerplasma nach A. F is c her (Pflügers Arch., 225, 737, I930) bestimmte Blutgerinnungsaktivität bleibt vollkommen erhalten. It has now been found that stable, sterilizable emulsions can be obtained can get when one emulsions of coagulant phosphatide amino acids clogs. Easily soluble amino acids are generally used as amino acids or mixtures of these, in particular glycocolla. The concentration of this added amino acids is chosen so that a blood isotonic solution is obtained. The emulsions obtained can be sterilized without modification. There is no flocculation. The hydrogen ion concentration remains before and same after sterilization. That on chicken plasma according to A. F is c her (Pflügers Arch., 225, 737, 1930) certain blood coagulation activity is completely retained.

Beispiel I 1 g gerinnungsaktives Phosphatidgemisch, gewonnen aus tierischem Blut z. B. durch Extraktion roter Blutkörperchen mit organischen Lösungsmitteln oder Lösungsmittelgemischen und Verdampfen der Lösungsmittel in an sich bekannter Weise, wird in I 1 Wasser dein verteilt. Man erhält eine stark opaleszente Emulsion, die nach Zusatz von 23 g Glykokoll ein PH von etwa 6 aufweist. Diese Emulsion wird in Ampullen abgefüllt und nach einer der üblichen Sterilisationsmethoden sterilisiert (z. B. Erhitzen in strömendem Wasserdampf von 1030 während 45 Minuten). Nach dem Erkalten erhält man eine bedeutend weniger opaleszente, stabile Emulsion vom pH etwa 6. Example I 1 g of coagulant phosphatide mixture obtained from animal blood e.g. B. by extracting red blood cells with organic solvents or solvent mixtures and evaporation of the solvents in a manner known per se Way, is distributed in I 1 water yours. A strongly opalescent emulsion is obtained, which has a pH of about 6 after adding 23 g of glycocolla. This emulsion will filled into ampoules and sterilized using one of the usual sterilization methods (e.g. heating in flowing steam of 1030 for 45 minutes). After this Cool down the result is a significantly less opalescent, stable emulsion with a pH of about 6.

Nach mehrmonatigem Stehen bei o' oder bei Zimmertemperatur treten keine Veränderungen auf und die Gerinnungsal;tivität ist dieselbe geblieben. Diese Lösung ist isotonisch zum Blutserum.After standing for several months, step at o 'or at room temperature no changes and the coagulation activity has remained the same. These Solution is isotonic to blood serum.

Beispiel z Io g Phosphatidgemisch (wie in Beispiel I gewonnen) wird in einer Lösung -von 26,5 g Alanin in 1 1 NVasser emulgiert. Nach Sterilisieren und Erkalten erhält man eine haltbare, gepufferte Emulsion von ausgezeichneter Gerinnungsaktivität. Example z Io g phosphatide mixture (as obtained in Example I) is Emulsified in a solution of 26.5 g of alanine in 1 liter of water. After sterilization and cooling, a long-lasting, buffered emulsion of excellent coagulation activity is obtained.

Claims (1)

PATENTANSPRUCH: Verfahren zur Herstellung stabiler sterilisierbarer Emulsionen von gerinnungsaktiven tierischen Phosphatiden, dadurch gekennzeichnet, daß man den Emulsionen Aminosäuren zusetzt. PATENT CLAIM: Process for the production of stable sterilizable Emulsions of coagulant animal phosphatides, characterized in that that one adds amino acids to the emulsions.
DE1939G0100246 1939-06-13 1939-06-17 Process for the production of stable, sterilizable emulsions of clot-activating phosphatides Expired DE700113C (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CH700113X 1939-06-13

Publications (1)

Publication Number Publication Date
DE700113C true DE700113C (en) 1940-12-13

Family

ID=4529955

Family Applications (1)

Application Number Title Priority Date Filing Date
DE1939G0100246 Expired DE700113C (en) 1939-06-13 1939-06-17 Process for the production of stable, sterilizable emulsions of clot-activating phosphatides

Country Status (1)

Country Link
DE (1) DE700113C (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE943792C (en) * 1949-02-18 1956-06-01 Organon Nv Process for the manufacture of injectable hormone preparations
EP0132821A2 (en) * 1983-07-25 1985-02-13 Eisai Co., Ltd. Aqueous solution containing lipid-soluble pharmaceutical substance and a process for preparing the same

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE943792C (en) * 1949-02-18 1956-06-01 Organon Nv Process for the manufacture of injectable hormone preparations
EP0132821A2 (en) * 1983-07-25 1985-02-13 Eisai Co., Ltd. Aqueous solution containing lipid-soluble pharmaceutical substance and a process for preparing the same
EP0132821A3 (en) * 1983-07-25 1985-07-10 Eisai Co., Ltd. Aqueous solution containing lipid-soluble pharmaceutical substance and a process for preparing the same

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