DE69943238D1 - Vorrichtung und verfahren zur electrophorese - Google Patents

Vorrichtung und verfahren zur electrophorese

Info

Publication number
DE69943238D1
DE69943238D1 DE69943238T DE69943238T DE69943238D1 DE 69943238 D1 DE69943238 D1 DE 69943238D1 DE 69943238 T DE69943238 T DE 69943238T DE 69943238 T DE69943238 T DE 69943238T DE 69943238 D1 DE69943238 D1 DE 69943238D1
Authority
DE
Germany
Prior art keywords
electrophoresis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69943238T
Other languages
German (de)
English (en)
Inventor
John E Wiktorowicz
Yefim M Raysberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Texas System
University of Texas at Austin
Original Assignee
University of Texas System
University of Texas at Austin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Texas System, University of Texas at Austin filed Critical University of Texas System
Application granted granted Critical
Publication of DE69943238D1 publication Critical patent/DE69943238D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Electrostatic Separation (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
DE69943238T 1998-05-22 1999-05-21 Vorrichtung und verfahren zur electrophorese Expired - Lifetime DE69943238D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/084,041 US6013165A (en) 1998-05-22 1998-05-22 Electrophoresis apparatus and method
PCT/US1999/011275 WO1999061901A1 (en) 1998-05-22 1999-05-21 Electrophoresis apparatus and method

Publications (1)

Publication Number Publication Date
DE69943238D1 true DE69943238D1 (de) 2011-04-14

Family

ID=22182516

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69943238T Expired - Lifetime DE69943238D1 (de) 1998-05-22 1999-05-21 Vorrichtung und verfahren zur electrophorese

Country Status (7)

Country Link
US (2) US6013165A (https=)
EP (1) EP1084396B9 (https=)
JP (1) JP4775872B2 (https=)
AU (1) AU758515B2 (https=)
CA (1) CA2332775C (https=)
DE (1) DE69943238D1 (https=)
WO (1) WO1999061901A1 (https=)

Families Citing this family (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6033850A (en) 1994-03-15 2000-03-07 Affymetrix, Inc. Electrochemical denaturation of double-stranded nucleic acid
EP0963435B1 (en) 1997-01-08 2008-05-28 Invitrogen Corporation Methods for production of proteins
US6143152A (en) * 1997-11-07 2000-11-07 The Regents Of The University Of California Microfabricated capillary array electrophoresis device and method
US6013165A (en) * 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
DE19826020C2 (de) * 1998-06-10 2000-11-02 Max Planck Gesellschaft Vorrichtung und Verfahren zur miniaturisierten, hochparallelen elektrophoretischen Trennung
SE9803224D0 (sv) * 1998-09-23 1998-09-23 Amersham Pharm Biotech Ab Method for separation of macromolecules
US6818112B2 (en) 1999-04-20 2004-11-16 Target Discovery, Inc. Protein separation via multidimensional electrophoresis
US6764817B1 (en) 1999-04-20 2004-07-20 Target Discovery, Inc. Methods for conducting metabolic analyses
JP2001033427A (ja) * 1999-07-16 2001-02-09 Hitachi Software Eng Co Ltd 電気泳動方法及び電気泳動装置
US7838222B2 (en) * 1999-07-26 2010-11-23 United States of America/ NIH Methods, devices and kits for multiplex blotting of biological samples from multi-well plates
US7214477B1 (en) 1999-07-26 2007-05-08 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Layered device with capture regions for cellular analysis
US6969615B2 (en) * 1999-07-26 2005-11-29 20/20 Genesystems, Inc. Methods, devices, arrays and kits for detecting and analyzing biomolecules
EP1218743B1 (en) 1999-07-26 2006-05-31 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Layered device with capture regions for cellular analysis
DE19935028C2 (de) * 1999-07-26 2003-03-20 Ibidi Gmbh Verfahren und Vorrichtungen zur elektrophoretischen Trennung von Partikeln, insbesondere von Makromolekülen
US7517442B1 (en) * 1999-08-09 2009-04-14 Life Technologies Corporation Facile method and apparatus for the analysis of biological macromolecules in two dimensions using common and familiar electrophoresis formats
US6613211B1 (en) * 1999-08-27 2003-09-02 Aclara Biosciences, Inc. Capillary electrokinesis based cellular assays
US6379519B1 (en) * 1999-09-01 2002-04-30 Mirador Dna Design Inc. Disposable thermoformed electrophoresis cassette
US6406604B1 (en) * 1999-11-08 2002-06-18 Norberto A. Guzman Multi-dimensional electrophoresis apparatus
US20040222097A1 (en) * 2000-03-13 2004-11-11 Koh Francis H. Channelled cassette for gel electrophoresis
GB0010957D0 (en) * 2000-05-05 2000-06-28 Novartis Ag Compound & method
US20060275851A1 (en) * 2000-07-26 2006-12-07 Emmert-Buck Michael R Layered peptide/antigen arrays - for high-throughput antibody screening of clinical samples
AU2001277247A1 (en) * 2000-08-10 2002-02-25 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
JP2004506221A (ja) * 2000-08-11 2004-02-26 インヴィトロジェン　コーポレーション 高度に均一な電気泳動用分子マーカー
US6743344B2 (en) * 2000-10-17 2004-06-01 Bio-Rad Laboratories, Inc. Coating of pre-cast electrophoresis slab gels
AU2002241480A1 (en) * 2000-11-17 2002-06-24 University Of Virginia Patent Foundation Method for orthogonal analyte stacking/injection systems in electrophoresis
AU4323602A (en) * 2000-11-20 2002-06-24 20 20 Gene Systems Inc Methods, devices, arrays and kits for detecting and analyzing biomolecules
US7070682B2 (en) * 2001-01-16 2006-07-04 Cheng Lee Microfluidic apparatus for performing gel protein extractions and methods for using the apparatus
US7309409B2 (en) * 2001-01-26 2007-12-18 Biocal Technology, Inc. Multi-channel bio-separation cartridge
DE10113257C1 (de) * 2001-03-19 2002-11-14 Inst Mikrotechnik Mainz Gmbh Elektrophoresevorrichtung und ihre Verwendung
WO2002084273A1 (en) * 2001-04-17 2002-10-24 Nextgen Sciences Ltd Electrophoretic separation system
US7641780B2 (en) 2001-05-01 2010-01-05 Calibrant Biosystems, Inc. Two-dimensional microfluidics for protein separations and gene analysis
US6974526B2 (en) * 2001-05-01 2005-12-13 Calibrant Biosystems, Inc. Plastic microfluidics enabling two-dimensional protein separations in proteome analysis
US6929730B2 (en) * 2001-05-01 2005-08-16 Cheng Sheng Lee Two dimensional microfluidic gene scanner
JP4236938B2 (ja) * 2001-05-10 2009-03-11 インビトロジェン コーポレイション 予め注型された水和可能分離媒体を電気泳動するための方法および装置
US7601251B2 (en) * 2001-05-10 2009-10-13 Life Technologies Corporation Methods and apparatus for low resistance electrophoresis of prior-cast, hydratable separation media
CN1549924A (zh) * 2001-07-16 2004-11-24 �����ʸ���˹�ع�˾ 通过等电点分析生物分子的缓冲液的阵列
GB0121189D0 (en) * 2001-08-31 2001-10-24 Diagnoswiss Sa Apparatus and method for separating an analyte
AU2002326845A1 (en) * 2001-09-07 2003-03-24 Applera Corporation Concentration and cleanup of nucleic acid samples
IL145649A0 (en) * 2001-09-25 2002-06-30 Nira Sciences Ltd Method and apparatus for real-time dynamic chemical analysis
WO2003038424A1 (en) * 2001-11-02 2003-05-08 Imperial College Innovations Limited Capillary electrophoresis microchip, system and method
WO2003062815A1 (en) * 2002-01-18 2003-07-31 Biocal Technology, Inc. Multi-segment cartridge for bio-separation with multiplexed fluorescence detection
GB2386954B (en) * 2002-02-19 2004-05-12 Nextgen Sciences Ltd Analyte separation system
WO2003092846A2 (en) * 2002-05-01 2003-11-13 Cheng Sheng Lee Plastic microfluidics enabling two-dimensional separations of biological molecules
WO2003092848A2 (en) * 2002-05-06 2003-11-13 Perkinelmer, Las, Inc. Separation process and dyes for use therewith
US6942775B1 (en) * 2002-05-24 2005-09-13 Owl Separation Systems, Inc. Vertical electrophoresis system
AU2003234885A1 (en) * 2002-06-07 2003-12-22 Picosep A/S Method and system for multi-stage isoelectric focussing
WO2004011144A2 (en) * 2002-07-29 2004-02-05 Dumas David P Transparent polymer support for electrophoresis and electrochromatography and related methods
CN1688886A (zh) * 2002-08-13 2005-10-26 独立行政法人农业.生物系特定产业技术研究机构 检测变应原蛋白质的方法
US20040112751A1 (en) * 2002-08-26 2004-06-17 Jongyoon Han Multidimensional electrophoresis and methods of making and using thereof
US20060096906A1 (en) * 2002-10-13 2006-05-11 Adam Rubin Microfluid biomolecule separation system
DE10252177A1 (de) * 2002-11-09 2004-06-09 Roche Diagnostics Gmbh Trennvorrichtung und Trennverfahren für biomolekulares Probenmaterial
US20060147926A1 (en) * 2002-11-25 2006-07-06 Emmert-Buck Michael R Method and apparatus for performing multiple simultaneous manipulations of biomolecules in a two-dimensional array
US20040149568A1 (en) * 2003-01-24 2004-08-05 Huang Lotien Richard Method for loading and unloading macro-molecules from microfluidic devices
WO2004072236A2 (en) * 2003-02-06 2004-08-26 The Board Of Trustees Of The University Of Illinois Methods and compositions for 3-d sodium dodecyl sulfate-polyacrylamide cube gel electrophoresis
US6969452B2 (en) * 2003-02-28 2005-11-29 Combisep, Inc. Two-dimensional protein separations using chromatofocusing and multiplexed capillary gel electrophoresis
US7147764B2 (en) * 2003-03-28 2006-12-12 Applera Corporation Dual electrode injection of analyte into a capillary electrophoretic device
CA2523189A1 (en) * 2003-04-22 2004-11-04 Molecular Technologies, Inc. System and method for synthesis of molecular imaging probes including fdg
US20050025741A1 (en) 2003-05-15 2005-02-03 Lau Aldrich N.K. Poly and copoly(N-vinylamide)s and their use in capillary electrophoresis
US7150813B2 (en) * 2003-06-12 2006-12-19 Palo Alto Research Center Incorporated Isoelectric focusing (IEF) of proteins with sequential and oppositely directed traveling waves in gel electrophoresis
US7250100B2 (en) * 2003-06-17 2007-07-31 Duke University Two dimensional electrophoresis cassette
US7258777B2 (en) * 2003-07-21 2007-08-21 Eksigent Technologies Llc Bridges for electroosmotic flow systems
US20050090013A1 (en) * 2003-10-28 2005-04-28 Joel Myerson Separation channel with transverse pump extraction
JP2007510935A (ja) * 2003-11-07 2007-04-26 プリンストン・バイオケミカルズ・インコーポレーテッド 多次元電気泳動装置
US8007724B2 (en) * 2003-11-07 2011-08-30 Princeton Biochemicals, Inc. Electrophoresis apparatus having at least one auxiliary buffer passage
WO2005089910A1 (en) * 2004-03-17 2005-09-29 Ciphergen Biosystems, Inc. Multi-compartment filter and method of filtering using same
US7402229B2 (en) * 2004-03-31 2008-07-22 Intel Corporation Fabrication and use of semipermeable membranes and gels for the control of electrolysis in a microfluidic device
US7491307B2 (en) * 2004-05-04 2009-02-17 Palo Alto Research Center Incorporated Portable bioagent concentrator
US7534336B2 (en) * 2004-05-04 2009-05-19 Palo Alto Research Center Incorporated Continuous flow particle concentrator
JP4441653B2 (ja) * 2005-08-31 2010-03-31 シャープ株式会社 自動化２次元電気泳動装置および装置構成器具
JP4728072B2 (ja) * 2005-09-05 2011-07-20 シャープ株式会社 電気泳動装置および装置構成器具
JP4734065B2 (ja) * 2005-09-05 2011-07-27 シャープ株式会社 電気泳動装置および装置構成器具
GB2440749B (en) * 2006-05-26 2011-04-06 Marc Baumann Multi-dimensional analysis
JP4316596B2 (ja) * 2006-09-13 2009-08-19 シャープ株式会社 サンプルの分析方法並びに分析装置
US8931644B2 (en) * 2006-11-30 2015-01-13 Palo Alto Research Center Incorporated Method and apparatus for splitting fluid flow in a membraneless particle separation system
US9862624B2 (en) 2007-11-07 2018-01-09 Palo Alto Research Center Incorporated Device and method for dynamic processing in water purification
US8276760B2 (en) * 2006-11-30 2012-10-02 Palo Alto Research Center Incorporated Serpentine structures for continuous flow particle separations
US9486812B2 (en) * 2006-11-30 2016-11-08 Palo Alto Research Center Incorporated Fluidic structures for membraneless particle separation
US10052571B2 (en) * 2007-11-07 2018-08-21 Palo Alto Research Center Incorporated Fluidic device and method for separation of neutrally buoyant particles
US9433880B2 (en) * 2006-11-30 2016-09-06 Palo Alto Research Center Incorporated Particle separation and concentration system
US20080220442A1 (en) * 2006-12-06 2008-09-11 Proteinics Difference detection methods using isoelectric focusing chips
US20080160629A1 (en) * 2007-01-02 2008-07-03 Calibrant Biosystems, Inc. Methods and systems for off-line multidimensional concentration and separation of biomolecules
US20080156080A1 (en) * 2007-01-02 2008-07-03 Calibrant Biosystems, Inc. Methods and systems for multidimensional concentration and separation of biomolecules using capillary isotachophoresis
AT504849B1 (de) * 2007-01-31 2011-03-15 Seibersdorf Labor Gmbh Visualisierung von elektrophoresegelen
WO2009046526A1 (en) * 2007-10-09 2009-04-16 Dalhousie University Apparatus for purifying molecules
US8282800B2 (en) * 2008-09-02 2012-10-09 Bio-Rad Laboratories, Inc. Hydrolysis-resistant polyacrylamide gels
TWI358539B (en) * 2008-12-09 2012-02-21 Univ Nat Taiwan Integrated electrophoresis device and operation th
US8282803B2 (en) 2009-03-25 2012-10-09 Bio-Rad Laboratories, Inc. Isoelectric focusing tray and electrode assembly for alternate gel strip orientations
CN102156160B (zh) * 2010-02-12 2013-05-29 陈翰民 二维蛋白质电泳提升测量精度的方法
US8586913B2 (en) 2011-01-10 2013-11-19 Schlumberger Technology Corporation Fluidic density measurements based on beta particles detection
US20140374260A1 (en) * 2012-02-07 2014-12-25 Sharp Kabushiki Kaisha Two-dimensional electrophoresis kit, method for manufacturing two-dimensional electrophoresis kit, two-dimensional electrophoresis method, and two-dimensional electrophoresis chip
WO2024086495A2 (en) * 2022-10-21 2024-04-25 Intabio, Llc Fixtures and devices for use in isoelectric focusing-mass spectrometry

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4417967A (en) * 1981-11-24 1983-11-29 Georgetown University Grooved gel
US4385974A (en) * 1982-06-24 1983-05-31 Jerry Shevitz Electrophoretic system and method for multidimensional analysis
US5360523A (en) * 1984-03-29 1994-11-01 Li-Cor, Inc. DNA sequencing
US4904632A (en) * 1987-06-19 1990-02-27 Pesek Joseph J Surface-modified chromatographic separation material
US5173159A (en) * 1988-09-06 1992-12-22 Bertin & Cie Multiple electrophoresis method for the controlled migration of macromolecules through rectangular gel plates
US4874490A (en) * 1988-11-04 1989-10-17 Bio-Rad Laboratories, Inc. Pre-cast gel systems for two-dimensional electrophoresis
US5017540A (en) * 1989-09-15 1991-05-21 Sandoval Junior E Silicon hydride surface intermediates for chemical separations apparatus
US5264101A (en) * 1989-11-06 1993-11-23 Applied Biosystems, Inc. Capillary electrophoresis molecular weight separation of biomolecules using a polymer-containing solution
US5885432A (en) * 1992-11-05 1999-03-23 Soane Biosciences Un-crosslinked polymeric media for electrophoresis
EP0653631B1 (de) * 1993-11-11 2003-05-14 Aclara BioSciences, Inc. Vorrichtung und Verfahren zur elektrophoretischen Trennung von fluiden Substanzgemischen
US5540826A (en) * 1995-03-15 1996-07-30 Protein Technologies, Inc. Multi-channel separation device
US5837116A (en) * 1996-07-12 1998-11-17 California Institute Of Technology Two dimensional electrophoresis apparatus
US5773645A (en) * 1997-05-05 1998-06-30 Bio-Rad Laboratories, Inc. Two-dimensional electrophoresis device
US6013165A (en) * 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method

Also Published As

Publication number Publication date
EP1084396A4 (en) 2006-09-06
JP2002516996A (ja) 2002-06-11
JP4775872B2 (ja) 2011-09-21
AU4092999A (en) 1999-12-13
EP1084396B9 (en) 2011-07-13
CA2332775A1 (en) 1999-12-02
WO1999061901A1 (en) 1999-12-02
EP1084396B1 (en) 2011-03-02
AU758515B2 (en) 2003-03-20
CA2332775C (en) 2009-01-06
US6013165A (en) 2000-01-11
US6214191B1 (en) 2001-04-10
EP1084396A1 (en) 2001-03-21

Similar Documents

Publication Publication Date Title
DE69943238D1 (de) Vorrichtung und verfahren zur electrophorese
DE69940804D1 (de) Verfahren und vorrichtung zur unterdrückung von abklingartefakten
DE69907878D1 (de) Verfahren und vorrichtung zur elektrokoagulation von flüssigkeiten
DE69903497D1 (de) Verfahren und Vorrichtung zur Unterdrückung von Resonanz
DE69804808D1 (de) Verfahren und vorrichtung zur reduzierung von verunreinigungen
DE69532091D1 (de) Verfahren und Vorrichtung zur Durchführung von Messungen
DE69530754D1 (de) Verfahren und vorrichtung zur kapillarentwässerung
DE69842100D1 (de) Verfahren und Vorrichtung zur Bildkodierung
EP0927880A4 (en) METHOD AND DEVICE FOR PREPARING SAMPLES
DE69621608D1 (de) Vorrichtung und verfahren für kapillarelektrophorese
DE69822687D1 (de) Vorrichtung und Verfahren zur Zusammenfassung
DE69805756D1 (de) Verfahren und vorrichtung zur analyse
DE69926784D1 (de) Vorrichtung und verfahren zur motorkontrolle
DE69840531D1 (de) Vorrichtung und verfahren zur ermittlung des gesichtsfeldes
DE69938403D1 (de) Verfahren und Vorrichtung zur Routenberechnung
DE69925155D1 (de) Verfahren und gerät zur klassifikation
DE19983717T1 (de) Vorrichtung und Verfahren zur Ausrichtung
DE69834320D1 (de) Verfahren und Vorrichtung zur Folgeschätzung
DE69818981D1 (de) Vorrichtung und verfahren zur kristallisation
DE69719477D1 (de) Vorrichtung und Verfahren zur Eingabe von Buchstaben
DE69724681D1 (de) Vorrichtung und Verfahren zur Analyse von Kristallstruktur
DE69942295D1 (de) Vorrichtung und verfahren zur informationsverarbeitung
DE69819227D1 (de) Vorrichtung und Verfahren zur Trübungsmessung
DE69714484D1 (de) Vorrichtung und Verfahren zur Koordinateneingabe
DE69926451D1 (de) Verfahren und Vorrichtung zur Unterdrückung von Mehrkanalechos