DE69933755D1 - Ef-tu mrna als marker für lebensfähige bakterien - Google Patents
Ef-tu mrna als marker für lebensfähige bakterienInfo
- Publication number
- DE69933755D1 DE69933755D1 DE69933755T DE69933755T DE69933755D1 DE 69933755 D1 DE69933755 D1 DE 69933755D1 DE 69933755 T DE69933755 T DE 69933755T DE 69933755 T DE69933755 T DE 69933755T DE 69933755 D1 DE69933755 D1 DE 69933755D1
- Authority
- DE
- Germany
- Prior art keywords
- bacteria
- present
- mrna
- amplification
- viability
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000894006 Bacteria Species 0.000 title abstract 3
- 239000003550 marker Substances 0.000 title 1
- 238000000034 method Methods 0.000 abstract 5
- 108091034117 Oligonucleotide Proteins 0.000 abstract 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 3
- 230000003321 amplification Effects 0.000 abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 3
- 102100030801 Elongation factor 1-alpha 1 Human genes 0.000 abstract 2
- 108010049977 Peptide Elongation Factor Tu Proteins 0.000 abstract 2
- 230000001580 bacterial effect Effects 0.000 abstract 2
- 108020004999 messenger RNA Proteins 0.000 abstract 2
- 230000035899 viability Effects 0.000 abstract 2
- 241000186362 Mycobacterium leprae Species 0.000 abstract 1
- 241000187479 Mycobacterium tuberculosis Species 0.000 abstract 1
- 206010036790 Productive cough Diseases 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 210000001124 body fluid Anatomy 0.000 abstract 1
- 239000010839 body fluid Substances 0.000 abstract 1
- 230000014670 detection of bacterium Effects 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
- 210000003802 sputum Anatomy 0.000 abstract 1
- 208000024794 sputum Diseases 0.000 abstract 1
- 201000008827 tuberculosis Diseases 0.000 abstract 1
- 210000002700 urine Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP98200184 | 1998-01-23 | ||
| EP98200184 | 1998-01-23 | ||
| PCT/EP1999/000323 WO1999037804A2 (en) | 1998-01-23 | 1999-01-19 | EF-Tu mRNA AS A MARKER FOR VIABILITY OF BACTERIA |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69933755D1 true DE69933755D1 (de) | 2006-12-07 |
| DE69933755T2 DE69933755T2 (de) | 2007-09-13 |
Family
ID=8233311
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69933755T Expired - Lifetime DE69933755T2 (de) | 1998-01-23 | 1999-01-19 | Ef-tu mrna als marker für lebensfähige bakterien |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6489110B1 (de) |
| EP (1) | EP1049809B1 (de) |
| JP (2) | JP4634604B2 (de) |
| AT (1) | ATE343642T1 (de) |
| AU (1) | AU2717699A (de) |
| CA (1) | CA2318726C (de) |
| DE (1) | DE69933755T2 (de) |
| ES (1) | ES2275336T3 (de) |
| WO (1) | WO1999037804A2 (de) |
| ZA (1) | ZA99362B (de) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2388461C (en) * | 1999-09-28 | 2013-06-11 | Infectio Diagnostic (I.D.I.) Inc. | Highly conserved genes and their use to generate probes and primers for the detection of microorganisms |
| EP1950313A3 (de) * | 2000-03-27 | 2010-12-08 | GlaxoSmithKline LLC | Detektion von lebensfähigen Agenzien |
| WO2002052034A1 (fr) * | 2000-12-26 | 2002-07-04 | Joji Oshima | Methodes de bioscopie et d'amplification d'acides nucleiques |
| BR0209282A (pt) * | 2001-04-30 | 2004-07-27 | Novologix Gmbh | Composição antibacteriana |
| AU2003260815A1 (en) * | 2002-08-08 | 2004-02-25 | University Of Lausanne | Method for the rapid assessment of the presence and viability of bacterial cells and use thereof________________________ |
| GB0324650D0 (en) * | 2003-10-22 | 2003-11-26 | Acolyte Biomedica Ltd | Using nucleic acids for clinical microbiology testing |
| US7309589B2 (en) * | 2004-08-20 | 2007-12-18 | Vironix Llc | Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing |
| AU2006274507B2 (en) * | 2005-07-27 | 2013-08-29 | Diagnostic Array Systems Pty Ltd | Method for the detection and/or identification of a microorganism |
| EP2363497A3 (de) * | 2005-07-27 | 2011-12-14 | Diagnostic Array Systems Pty Ltd | Verfahren zum Nachweis und/oder zur Identifizierung eines Mikroorganismus |
| JP5593037B2 (ja) * | 2009-03-12 | 2014-09-17 | 東洋水産株式会社 | 細菌測定用プライマーおよび細菌測定方法 |
| US11441167B2 (en) * | 2019-11-20 | 2022-09-13 | Roche Molecular Systems, Inc. | Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL71715A0 (en) * | 1983-08-05 | 1984-09-30 | Miles Lab | Method,gene probes and test kit for detecting bacteria by nucleic acid hybridization |
| US5130238A (en) * | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| WO1996006191A2 (en) | 1994-08-18 | 1996-02-29 | Akzo Nobel N.V. | Primers and probes for the amplification and detection of cmv nucleic acid |
| US5994066A (en) | 1995-09-11 | 1999-11-30 | Infectio Diagnostic, Inc. | Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| JPH09248186A (ja) * | 1996-03-15 | 1997-09-22 | Oozeki Kk | ラクトバチルス属微生物のポリペプチド鎖延長因子遺伝子Ｔｕおよびその利用 |
| US6444444B1 (en) | 1996-07-10 | 2002-09-03 | Aventis Pasteur Limited | Genes encoding mycobacterial proteins associated with cell binding and cell entry and uses thereof |
| FR2755145B1 (fr) * | 1996-10-28 | 1999-01-15 | Gervais Danone Co | Procede de mise en evidence de contaminants microbiologiques vivants dans un echantillon de produit a usage alimentaire |
-
1999
- 1999-01-19 WO PCT/EP1999/000323 patent/WO1999037804A2/en active IP Right Grant
- 1999-01-19 DE DE69933755T patent/DE69933755T2/de not_active Expired - Lifetime
- 1999-01-19 AU AU27176/99A patent/AU2717699A/en not_active Abandoned
- 1999-01-19 ES ES99907386T patent/ES2275336T3/es not_active Expired - Lifetime
- 1999-01-19 EP EP99907386A patent/EP1049809B1/de not_active Expired - Lifetime
- 1999-01-19 CA CA002318726A patent/CA2318726C/en not_active Expired - Lifetime
- 1999-01-19 AT AT99907386T patent/ATE343642T1/de not_active IP Right Cessation
- 1999-01-19 US US09/600,770 patent/US6489110B1/en not_active Expired - Lifetime
- 1999-01-19 JP JP2000528711A patent/JP4634604B2/ja not_active Expired - Lifetime
- 1999-01-19 ZA ZA9900362A patent/ZA99362B/xx unknown
-
2010
- 2010-10-06 JP JP2010226408A patent/JP2011050387A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DE69933755T2 (de) | 2007-09-13 |
| JP2002500893A (ja) | 2002-01-15 |
| WO1999037804A2 (en) | 1999-07-29 |
| CA2318726C (en) | 2009-07-28 |
| EP1049809A2 (de) | 2000-11-08 |
| JP4634604B2 (ja) | 2011-02-16 |
| AU2717699A (en) | 1999-08-09 |
| US6489110B1 (en) | 2002-12-03 |
| ES2275336T3 (es) | 2007-06-01 |
| JP2011050387A (ja) | 2011-03-17 |
| EP1049809B1 (de) | 2006-10-25 |
| WO1999037804A3 (en) | 1999-11-11 |
| CA2318726A1 (en) | 1999-07-29 |
| ATE343642T1 (de) | 2006-11-15 |
| ZA99362B (en) | 1999-07-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Achberger et al. | Microbial community structure of subglacial lake Whillans, West Antarctica | |
| Águila-Arcos et al. | Biofilm-forming clinical Staphylococcus isolates harbor horizontal transfer and antibiotic resistance genes | |
| Schultz et al. | Modeling the sensitivity of field surveys for detection of environmental DNA (eDNA) | |
| Walters et al. | Detection of Bacteroidales fecal indicators and the zoonotic pathogens E. coli O157: H7, Salmonella, and Campylobacter in river water | |
| Fode‐Vaughan et al. | Direct PCR detection of Escherichia coli O157: H7 | |
| Ahmed et al. | Evaluation of Bacteroides markers for the detection of human faecal pollution | |
| Rondini et al. | Development and application of real-time PCR assay for quantification of Mycobacterium ulcerans DNA | |
| Amberg et al. | Improving efficiency and reliability of environmental DNA analysis for silver carp | |
| Guimaraes-Peres et al. | Comparison of two PCRs for detection of Mycobacterium ulcerans | |
| DE69933755D1 (de) | Ef-tu mrna als marker für lebensfähige bakterien | |
| BR0211155A (pt) | Amplificação de fragmentos de ácido nucléico com uso de agentes de corte | |
| DE60210621D1 (de) | Universelle methode und zusammensetzung zur schnellen lysierung von zellen zur freisetzung von nukleinsäuren und ihre detektion | |
| Williams et al. | Comparing CRISPR‐Cas and qPCR eDNA assays for the detection of Atlantic salmon (Salmo salar L.) | |
| ATE368125T1 (de) | Verfahren zur erkennung von einzel-nukleotid- polymorphismen | |
| Chern et al. | Evaluation of quantitative polymerase chain reaction assays targeting Mycobacterium avium, M. intracellulare, and M. avium subspecies paratuberculosis in drinking water biofilms | |
| ATE329061T1 (de) | Verfahren zur reduzierung unspezifischer amplifikation bei pcr | |
| Gumber et al. | Comparison of BACTEC 460 and MGIT 960 systems for the culture of Mycobacterium avium subsp. paratuberculosis S strain and observations on the effect of inclusion of ampicillin in culture media to reduce contamination | |
| DK0887425T3 (da) | Multiplekset PCR assay til detektering af dissemineret Mycobacterium avium kompleks infektion | |
| Kumanan et al. | A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples | |
| ATE519858T1 (de) | Biologischer bodendetektor und verfahren zur verwendung davon | |
| JP2013521804A (ja) | 室温での溶解のためのアクロモペプチダーゼの使用 | |
| Cangelosi et al. | Molecular detection of viable bacterial pathogens in water by ratiometric pre-rRNA analysis | |
| Huby-Chilton et al. | Single-strand conformation polymorphism (SSCP) analysis as a new diagnostic tool to distinguish dorsal-spined larvae of the Elaphostrongylinae (Nematoda: Protostrongylidae) from cervids | |
| Hrušková et al. | Multiplex polymerase chain reaction using ethidium monoazide and propidium monoazide for distinguishing viable and dead cells of arcobacters in biofilm | |
| WO2007034118A8 (fr) | Procede de detection, dans un echantillon biologique de mycobacteries, et en particulier de mycobacteries caracteristiques de la tuberculose |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |