ZA99362B - EF-Tu mRNA as a marker for viability of bacteria. - Google Patents
EF-Tu mRNA as a marker for viability of bacteria.Info
- Publication number
- ZA99362B ZA99362B ZA9900362A ZA99362A ZA99362B ZA 99362 B ZA99362 B ZA 99362B ZA 9900362 A ZA9900362 A ZA 9900362A ZA 99362 A ZA99362 A ZA 99362A ZA 99362 B ZA99362 B ZA 99362B
- Authority
- ZA
- South Africa
- Prior art keywords
- bacteria
- present
- viability
- mrna
- amplification
- Prior art date
Links
- 241000894006 Bacteria Species 0.000 title abstract 3
- 102100030801 Elongation factor 1-alpha 1 Human genes 0.000 title abstract 3
- 108010049977 Peptide Elongation Factor Tu Proteins 0.000 title abstract 3
- 108020004999 messenger RNA Proteins 0.000 title abstract 3
- 230000035899 viability Effects 0.000 title abstract 3
- 239000003550 marker Substances 0.000 title 1
- 238000000034 method Methods 0.000 abstract 5
- 108091034117 Oligonucleotide Proteins 0.000 abstract 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 3
- 230000003321 amplification Effects 0.000 abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 3
- 230000001580 bacterial effect Effects 0.000 abstract 2
- 241000186362 Mycobacterium leprae Species 0.000 abstract 1
- 241000187479 Mycobacterium tuberculosis Species 0.000 abstract 1
- 206010036790 Productive cough Diseases 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 210000001124 body fluid Anatomy 0.000 abstract 1
- 239000010839 body fluid Substances 0.000 abstract 1
- 230000014670 detection of bacterium Effects 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
- 210000003802 sputum Anatomy 0.000 abstract 1
- 208000024794 sputum Diseases 0.000 abstract 1
- 201000008827 tuberculosis Diseases 0.000 abstract 1
- 210000002700 urine Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP98200184 | 1998-01-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA99362B true ZA99362B (en) | 1999-07-19 |
Family
ID=8233311
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA9900362A ZA99362B (en) | 1998-01-23 | 1999-01-19 | EF-Tu mRNA as a marker for viability of bacteria. |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6489110B1 (xx) |
| EP (1) | EP1049809B1 (xx) |
| JP (2) | JP4634604B2 (xx) |
| AT (1) | ATE343642T1 (xx) |
| AU (1) | AU2717699A (xx) |
| CA (1) | CA2318726C (xx) |
| DE (1) | DE69933755T2 (xx) |
| ES (1) | ES2275336T3 (xx) |
| WO (1) | WO1999037804A2 (xx) |
| ZA (1) | ZA99362B (xx) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR0014370A (pt) * | 1999-09-28 | 2002-11-05 | Infectio Diagnostic Inc | Genes altamente conservados e seu uso para gerar sondas de ácido nucléico e preparadores de amplificação especìficos em espécie, especìfico em gênero, especìficos em famìlia, especìficos em grupo e universais para rapidamente detectar e identificar microorganismos algais, amebianos, bacterianos, fúngicos e parasitas de espécimes clìnicos para diagnose |
| EP1950313A3 (en) * | 2000-03-27 | 2010-12-08 | GlaxoSmithKline LLC | Detection of viable agents |
| WO2002052034A1 (fr) * | 2000-12-26 | 2002-07-04 | Joji Oshima | Methodes de bioscopie et d'amplification d'acides nucleiques |
| CA2445995A1 (en) * | 2001-04-30 | 2002-11-07 | Novologix Gmbh | Antibacterial agent |
| EP1529117A2 (en) * | 2002-08-08 | 2005-05-11 | University of Lausanne | Method for the rapid assessment of the presence and viability of bacterial cells and use thereof |
| GB0324650D0 (en) * | 2003-10-22 | 2003-11-26 | Acolyte Biomedica Ltd | Using nucleic acids for clinical microbiology testing |
| US7309589B2 (en) * | 2004-08-20 | 2007-12-18 | Vironix Llc | Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing |
| JP5138590B2 (ja) * | 2005-07-27 | 2013-02-06 | ディアグノスティック アレイ システムズ ピーティーワイ エルティーディー | 微生物の検出及び／又は同定方法 |
| AU2006274507B2 (en) * | 2005-07-27 | 2013-08-29 | Diagnostic Array Systems Pty Ltd | Method for the detection and/or identification of a microorganism |
| JP5593037B2 (ja) * | 2009-03-12 | 2014-09-17 | 東洋水産株式会社 | 細菌測定用プライマーおよび細菌測定方法 |
| US11441167B2 (en) | 2019-11-20 | 2022-09-13 | Roche Molecular Systems, Inc. | Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL71715A0 (en) | 1983-08-05 | 1984-09-30 | Miles Lab | Method,gene probes and test kit for detecting bacteria by nucleic acid hybridization |
| US5130238A (en) * | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| KR100399715B1 (ko) * | 1994-08-18 | 2003-12-18 | 악조 노벨 엔.브이. | Cmv핵산증폭 및 검출용 프라이머와 프로브 |
| US5994066A (en) | 1995-09-11 | 1999-11-30 | Infectio Diagnostic, Inc. | Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| JPH09248186A (ja) * | 1996-03-15 | 1997-09-22 | Oozeki Kk | ラクトバチルス属微生物のポリペプチド鎖延長因子遺伝子Ｔｕおよびその利用 |
| US6444444B1 (en) * | 1996-07-10 | 2002-09-03 | Aventis Pasteur Limited | Genes encoding mycobacterial proteins associated with cell binding and cell entry and uses thereof |
| FR2755145B1 (fr) * | 1996-10-28 | 1999-01-15 | Gervais Danone Co | Procede de mise en evidence de contaminants microbiologiques vivants dans un echantillon de produit a usage alimentaire |
-
1999
- 1999-01-19 EP EP99907386A patent/EP1049809B1/en not_active Expired - Lifetime
- 1999-01-19 JP JP2000528711A patent/JP4634604B2/ja not_active Expired - Lifetime
- 1999-01-19 WO PCT/EP1999/000323 patent/WO1999037804A2/en active IP Right Grant
- 1999-01-19 CA CA002318726A patent/CA2318726C/en not_active Expired - Lifetime
- 1999-01-19 DE DE69933755T patent/DE69933755T2/de not_active Expired - Lifetime
- 1999-01-19 ES ES99907386T patent/ES2275336T3/es not_active Expired - Lifetime
- 1999-01-19 AT AT99907386T patent/ATE343642T1/de not_active IP Right Cessation
- 1999-01-19 US US09/600,770 patent/US6489110B1/en not_active Expired - Lifetime
- 1999-01-19 AU AU27176/99A patent/AU2717699A/en not_active Abandoned
- 1999-01-19 ZA ZA9900362A patent/ZA99362B/xx unknown
-
2010
- 2010-10-06 JP JP2010226408A patent/JP2011050387A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP1049809B1 (en) | 2006-10-25 |
| ES2275336T3 (es) | 2007-06-01 |
| US6489110B1 (en) | 2002-12-03 |
| CA2318726C (en) | 2009-07-28 |
| JP2011050387A (ja) | 2011-03-17 |
| JP2002500893A (ja) | 2002-01-15 |
| WO1999037804A3 (en) | 1999-11-11 |
| WO1999037804A2 (en) | 1999-07-29 |
| DE69933755T2 (de) | 2007-09-13 |
| CA2318726A1 (en) | 1999-07-29 |
| EP1049809A2 (en) | 2000-11-08 |
| JP4634604B2 (ja) | 2011-02-16 |
| ATE343642T1 (de) | 2006-11-15 |
| DE69933755D1 (de) | 2006-12-07 |
| AU2717699A (en) | 1999-08-09 |
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