DE69831958D1 - Primordiale keimzellinie aus vögeln und verfahren zu deren langzeitkultivierung - Google Patents

Primordiale keimzellinie aus vögeln und verfahren zu deren langzeitkultivierung

Info

Publication number
DE69831958D1
DE69831958D1 DE69831958T DE69831958T DE69831958D1 DE 69831958 D1 DE69831958 D1 DE 69831958D1 DE 69831958 T DE69831958 T DE 69831958T DE 69831958 T DE69831958 T DE 69831958T DE 69831958 D1 DE69831958 D1 DE 69831958D1
Authority
DE
Germany
Prior art keywords
primordial
birds
germany
long
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69831958T
Other languages
English (en)
Other versions
DE69831958T2 (de
Inventor
De Leon Abel Ponce
Catherine Blackwell
Ying Gao
M Robl
L Stice
Joseph Jerry
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Massachusetts UMass
Original Assignee
University of Massachusetts UMass
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/905,773 external-priority patent/US6156569A/en
Application filed by University of Massachusetts UMass filed Critical University of Massachusetts UMass
Application granted granted Critical
Publication of DE69831958D1 publication Critical patent/DE69831958D1/de
Publication of DE69831958T2 publication Critical patent/DE69831958T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027New or modified breeds of vertebrates
    • A01K67/0271Chimeric vertebrates, e.g. comprising exogenous cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0608Germ cells
    • C12N5/0611Primordial germ cells, e.g. embryonic germ cells [EG]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00Animals characterised by species
    • A01K2227/30Bird
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/105Insulin-like growth factors [IGF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/125Stem cell factor [SCF], c-kit ligand [KL]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20Cytokines; Chemokines
    • C12N2501/23Interleukins [IL]
    • C12N2501/235Leukemia inhibitory factor [LIF]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Catching Or Destruction (AREA)
  • Fodder In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69831958T 1997-08-04 1998-08-04 Primordiale keimzellinie aus vögeln und verfahren zu deren langzeitkultivierung Expired - Fee Related DE69831958T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US905773 1992-06-29
US08/905,773 US6156569A (en) 1997-08-04 1997-08-04 Prolonged culturing of avian primordial germ cells (PGCs) using specific growth factors, use thereof to produce chimeric avians
US12762498A 1998-08-03 1998-08-03
US127624 1998-08-03
PCT/US1998/015635 WO1999006533A1 (en) 1997-08-04 1998-08-04 Avian primordial germ cell (pgc) cell line and a method for long term culturing thereof

Publications (2)

Publication Number Publication Date
DE69831958D1 true DE69831958D1 (de) 2006-03-02
DE69831958T2 DE69831958T2 (de) 2006-07-27

Family

ID=26825804

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69831958T Expired - Fee Related DE69831958T2 (de) 1997-08-04 1998-08-04 Primordiale keimzellinie aus vögeln und verfahren zu deren langzeitkultivierung

Country Status (12)

Country Link
US (1) US20040226058A1 (de)
EP (2) EP1616943A1 (de)
JP (1) JP2003532364A (de)
CN (1) CN1195844C (de)
AT (1) ATE307196T1 (de)
AU (1) AU736087B2 (de)
BR (1) BR9811830A (de)
CA (1) CA2300380A1 (de)
DE (1) DE69831958T2 (de)
ES (1) ES2252851T3 (de)
NZ (1) NZ502712A (de)
WO (1) WO1999006533A1 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100378747B1 (ko) * 1999-02-11 2003-04-07 한미약품공업 주식회사 조류의 다능성 배자생식세포주 및 이의 제조방법
US6333192B1 (en) 1999-08-09 2001-12-25 North Carolina State University Method of producing an undifferentiated avian cell culture using avian primordial germ cells
US6354242B1 (en) 2000-03-23 2002-03-12 North Carolina State University Methods for gamete production in birds
BR0211895A (pt) 2001-08-13 2006-04-04 Embrex Inc métodos de injetar um ovo avìcola, injetar uma pluralidade de ovos avìculas, de produzir uma ave quimérica e uma ave transgênica
FR2836924B1 (fr) 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
KR100569168B1 (ko) * 2003-08-08 2006-04-07 (주)아비코아생명공학연구소 조류 정원줄기세포의 배양방법 및 이에 의해 수득한 조류정원줄기세포
US20060174362A1 (en) 2005-02-01 2006-08-03 Origen Therapeutics, Inc. Long-term culture of avian primordial germ cells (PGCs)
CN100366734C (zh) * 2006-03-02 2008-02-06 扬州大学 一种鸡精原细胞分离纯化方法
EP2054507A1 (de) 2006-08-09 2009-05-06 Vivalis Verfahren zur herstellung transgener vögel unter verwendung embryonaler stammzellen
CN100443588C (zh) * 2006-12-11 2008-12-17 中国农业科学院北京畜牧兽医研究所 静宁鸡胚成纤维细胞系及其培养方法
EP1985305A1 (de) 2007-04-24 2008-10-29 Vivalis Aus Entenembryonen gewonnene Stammzellkulturen zur Herstellung von Impfstoffen gegen Viren
AU2010282508B2 (en) 2009-08-13 2015-05-28 Crystal Bioscience Inc. Transgenic animal for production of antibodies having minimal CDRs
US8865462B2 (en) * 2010-01-20 2014-10-21 Crystal Bioscience Inc. Sustained culture of avian gonocytes
AU2013204327B2 (en) 2012-04-20 2016-09-01 Aviagen Cell transfection method
CN103674653B (zh) * 2013-12-19 2016-08-24 浙江省农业科学院 一种原始生殖细胞的爬片方法
WO2016091272A1 (en) * 2014-12-12 2016-06-16 Central Veterinary Research Laboratory System and method for establishing a long-term culture of avian primordial germ cells and uses thereof
CA3128360A1 (en) 2019-03-05 2020-09-10 Yuval CINNAMON Genome-edited birds
CN111500531A (zh) * 2020-05-15 2020-08-07 扬州大学 一种鸡PGCs在体外长期培养的方法
CN116210647A (zh) * 2023-02-03 2023-06-06 威奥福生物科技(宁波)有限公司 一种使用原始生殖细胞干细胞系扩繁白羽肉鸡的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2726003B1 (fr) * 1994-10-21 2002-10-18 Agronomique Inst Nat Rech Milieu de culture de cellules embryonnaires totipotentes aviaires, procede de culture de ces cellules, et cellules embryonnaires totipotentes aviaires
US6156569A (en) * 1997-08-04 2000-12-05 University Of Massachusetts Office Of Vice Chancellor For Research At Amherst Prolonged culturing of avian primordial germ cells (PGCs) using specific growth factors, use thereof to produce chimeric avians
US6140118A (en) * 1999-08-11 2000-10-31 Wisconsin Alumni Research Foundation Avian blastodermal cell lines

Also Published As

Publication number Publication date
CN1195844C (zh) 2005-04-06
CN1273600A (zh) 2000-11-15
EP1007633A4 (de) 2002-06-05
CA2300380A1 (en) 1999-02-11
DE69831958T2 (de) 2006-07-27
JP2003532364A (ja) 2003-11-05
NZ502712A (en) 2003-01-31
AU8759398A (en) 1999-02-22
WO1999006533A1 (en) 1999-02-11
ES2252851T3 (es) 2006-05-16
EP1007633B1 (de) 2005-10-19
EP1007633A1 (de) 2000-06-14
EP1616943A1 (de) 2006-01-18
AU736087B2 (en) 2001-07-26
US20040226058A1 (en) 2004-11-11
BR9811830A (pt) 2000-08-15
ATE307196T1 (de) 2005-11-15

Similar Documents

Publication Publication Date Title
DE69831958D1 (de) Primordiale keimzellinie aus vögeln und verfahren zu deren langzeitkultivierung
EP1019491A4 (de) Herstellung embryonischer vogel keimzellinien durch verlängerte kultivierung von pgcs, verwendung desselben zur klonierung und chimerisierung
DE69535618D1 (de) Rekombinanter lebender Vogel-Impfstoff, der ein Hühne-Herpesvirus Vektor gebraucht
DE3782459D1 (de) Automatisierte hochgeschwindigkeitsinjektionsvorrichtung und verfahren fuer vogelembryos.
DK335388D0 (da) Fremgangsmaade til dyrkning af embryoner
DE3867743D1 (de) Benzamid-derivat, verfahren zu dessen herstellung und pflanzenwachstumsregulator.
ATE255419T1 (de) Für den vogelembryo geeignete multivalente impfstoffe
DE3881044T2 (de) Überzüge aus sulfonierten polymerisaten zur gesteuerten nährstoffabgabe.
DE60035863D1 (de) Mykotoxin-resistente transgene Weizenpflanze, Verfahren zur Herstellung und Verwendung derselben
DE58901183D1 (de) Flexible fuehrungsschiene und verfahren zur deren herstellung.
DE69209734T2 (de) Dithiocarbaminsäure salze, verfahren zur herstellung und verfahren zur herstellung von isothiocyanaten aus diesen salzen
DE69117846D1 (de) Einstufiges Kulturverfahren für Vogelembryos
DE69835812D1 (de) Anwendungsmoeglichkeiten der maennlichen sterilitaet von pflanzen
DE69821053T2 (de) Verfahren zur Herstellung von 2,2,6,6-Tetramethyl-4-piperidyl-Gruppen enthaltenden Polytriazinen
AT383253B (de) N-phenylpyrazolderivate enthaltende herbizidformulierungen, deren verwendung und verfahren zur bekaempfung des unkrautwachstums
DE69103907T2 (de) Verfahren zur herstellung von pigmentierten nylonfasern.
DE69210420T2 (de) Verfahren und vorrichtung zum ernten von pflanzen
DE60025096D1 (de) Verfahren zur herstellung von n,n'-dialkylalkandiaminen
DE3885592D1 (de) Verfahren zur herstellung von natürlichem methyl-anthranilat.
DE69811599D1 (de) Tintenstrahltinte, Verfahren zur Markierung von Eierschalen und markierte Eier
DE69528901T2 (de) Wasserdispergierbare pfropfcopolymere auf acrylbasis, ein verfahren zur herstellung und wässrige anstrichmittel
DE58900031D1 (de) Verfahren zur herstellung von natriumpolysulfiden aus den elementen natrium und schwefel.
ES1014567Y (es) Grupo integrado autonomo para riegos y fertilizacion controlados.
ATE127458T1 (de) Anlage und verfahren zur herstellung von cyanursäure.
FI963021A (fi) Kasvien viljelymenetelmä

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: UNIVERSITY OF MASSACHUSETTS INSTITUTION OF HIGHER

8339 Ceased/non-payment of the annual fee