DE69829860T2 - Spezies-übergreifendes anfärben von chromosomen - Google Patents
Spezies-übergreifendes anfärben von chromosomen Download PDFInfo
- Publication number
- DE69829860T2 DE69829860T2 DE69829860T DE69829860T DE69829860T2 DE 69829860 T2 DE69829860 T2 DE 69829860T2 DE 69829860 T DE69829860 T DE 69829860T DE 69829860 T DE69829860 T DE 69829860T DE 69829860 T2 DE69829860 T2 DE 69829860T2
- Authority
- DE
- Germany
- Prior art keywords
- chromosome
- chromosomes
- probes
- human
- species
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000000349 chromosome Anatomy 0.000 title claims abstract description 253
- 239000000523 sample Substances 0.000 claims abstract description 181
- 238000000034 method Methods 0.000 claims abstract description 45
- 208000031404 Chromosome Aberrations Diseases 0.000 claims abstract description 23
- 231100000005 chromosome aberration Toxicity 0.000 claims abstract description 15
- 241000282414 Homo sapiens Species 0.000 claims description 60
- 238000009396 hybridization Methods 0.000 claims description 42
- 241000894007 species Species 0.000 claims description 34
- 241001147404 Nomascus concolor Species 0.000 claims description 21
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 16
- 238000007901 in situ hybridization Methods 0.000 claims description 16
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 claims description 12
- 241000251539 Vertebrata <Metazoa> Species 0.000 claims description 8
- 229960002685 biotin Drugs 0.000 claims description 8
- 235000020958 biotin Nutrition 0.000 claims description 8
- 239000011616 biotin Substances 0.000 claims description 8
- 230000001594 aberrant effect Effects 0.000 claims description 6
- 241001465754 Metazoa Species 0.000 abstract description 18
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 11
- 210000003917 human chromosome Anatomy 0.000 description 43
- 241000282620 Hylobates sp. Species 0.000 description 36
- 239000003973 paint Substances 0.000 description 28
- 108020004414 DNA Proteins 0.000 description 24
- 238000010422 painting Methods 0.000 description 21
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 20
- 241000282623 Symphalangus syndactylus Species 0.000 description 17
- 230000031864 metaphase Effects 0.000 description 17
- 210000004027 cell Anatomy 0.000 description 16
- 238000003752 polymerase chain reaction Methods 0.000 description 15
- 239000000203 mixture Substances 0.000 description 14
- 238000002360 preparation method Methods 0.000 description 14
- 238000010186 staining Methods 0.000 description 14
- 230000008707 rearrangement Effects 0.000 description 13
- 238000004458 analytical method Methods 0.000 description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 11
- 230000003321 amplification Effects 0.000 description 10
- 238000003199 nucleic acid amplification method Methods 0.000 description 10
- 230000002759 chromosomal effect Effects 0.000 description 9
- 238000002372 labelling Methods 0.000 description 9
- 150000007523 nucleic acids Chemical class 0.000 description 9
- 239000013615 primer Substances 0.000 description 9
- 108091034117 Oligonucleotide Proteins 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 239000003298 DNA probe Substances 0.000 description 7
- 208000037516 chromosome inversion disease Diseases 0.000 description 7
- 238000001514 detection method Methods 0.000 description 7
- 238000000684 flow cytometry Methods 0.000 description 7
- 238000003384 imaging method Methods 0.000 description 7
- 108020004707 nucleic acids Proteins 0.000 description 7
- 102000039446 nucleic acids Human genes 0.000 description 7
- 238000001228 spectrum Methods 0.000 description 7
- 206010028980 Neoplasm Diseases 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 108091033319 polynucleotide Proteins 0.000 description 6
- 102000040430 polynucleotide Human genes 0.000 description 6
- 239000002157 polynucleotide Substances 0.000 description 6
- 230000005945 translocation Effects 0.000 description 6
- 241000282693 Cercopithecidae Species 0.000 description 5
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 5
- 241000288906 Primates Species 0.000 description 5
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 5
- 201000011510 cancer Diseases 0.000 description 5
- 230000002559 cytogenic effect Effects 0.000 description 5
- 238000012217 deletion Methods 0.000 description 5
- 230000037430 deletion Effects 0.000 description 5
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 5
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 5
- 108090001008 Avidin Proteins 0.000 description 4
- 206010008805 Chromosomal abnormalities Diseases 0.000 description 4
- 241000282597 Hylobates Species 0.000 description 4
- 241000282596 Hylobatidae Species 0.000 description 4
- 101100384865 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cot-1 gene Proteins 0.000 description 4
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 4
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 4
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 239000002853 nucleic acid probe Substances 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 230000017105 transposition Effects 0.000 description 4
- BUOYTFVLNZIELF-UHFFFAOYSA-N 2-phenyl-1h-indole-4,6-dicarboximidamide Chemical compound N1C2=CC(C(=N)N)=CC(C(N)=N)=C2C=C1C1=CC=CC=C1 BUOYTFVLNZIELF-UHFFFAOYSA-N 0.000 description 3
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 3
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 208000036878 aneuploidy Diseases 0.000 description 3
- 231100001075 aneuploidy Toxicity 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 230000008711 chromosomal rearrangement Effects 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 230000000875 corresponding effect Effects 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 230000016507 interphase Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- NALREUIWICQLPS-UHFFFAOYSA-N 7-imino-n,n-dimethylphenothiazin-3-amine;hydrochloride Chemical compound [Cl-].C1=C(N)C=C2SC3=CC(=[N+](C)C)C=CC3=NC2=C1 NALREUIWICQLPS-UHFFFAOYSA-N 0.000 description 2
- 241000282672 Ateles sp. Species 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- 241000282552 Chlorocebus aethiops Species 0.000 description 2
- 108020001019 DNA Primers Proteins 0.000 description 2
- 108020003215 DNA Probes Proteins 0.000 description 2
- 239000003155 DNA primer Substances 0.000 description 2
- 201000010374 Down Syndrome Diseases 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 241000282575 Gorilla Species 0.000 description 2
- 108091093105 Nuclear DNA Proteins 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 241000282577 Pan troglodytes Species 0.000 description 2
- 108010004729 Phycoerythrin Proteins 0.000 description 2
- 210000002593 Y chromosome Anatomy 0.000 description 2
- 239000004568 cement Substances 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000013611 chromosomal DNA Substances 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 238000004163 cytometry Methods 0.000 description 2
- 229960000633 dextran sulfate Drugs 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
- 238000000799 fluorescence microscopy Methods 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 230000003426 interchromosomal effect Effects 0.000 description 2
- 239000000891 luminescent agent Substances 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 210000005105 peripheral blood lymphocyte Anatomy 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 230000031877 prophase Effects 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- UKOBAUFLOGFCMV-UHFFFAOYSA-N quinacrine mustard Chemical compound C1=C(Cl)C=CC2=C(NC(C)CCCN(CCCl)CCCl)C3=CC(OC)=CC=C3N=C21 UKOBAUFLOGFCMV-UHFFFAOYSA-N 0.000 description 2
- 230000003252 repetitive effect Effects 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- UBOKASXZHPZFRZ-UHFFFAOYSA-N 2-phenyl-1h-indole-4,6-diamine Chemical compound N1C2=CC(N)=CC(N)=C2C=C1C1=CC=CC=C1 UBOKASXZHPZFRZ-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000282514 Alouatta Species 0.000 description 1
- 241000282709 Aotus trivirgatus Species 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 241001515796 Cebinae Species 0.000 description 1
- 241000282551 Cercopithecus Species 0.000 description 1
- 241000282994 Cervidae Species 0.000 description 1
- 206010011385 Cri-du-chat syndrome Diseases 0.000 description 1
- OHOQEZWSNFNUSY-UHFFFAOYSA-N Cy3-bifunctional dye zwitterion Chemical compound O=C1CCC(=O)N1OC(=O)CCCCCN1C2=CC=C(S(O)(=O)=O)C=C2C(C)(C)C1=CC=CC(C(C1=CC(=CC=C11)S([O-])(=O)=O)(C)C)=[N+]1CCCCCC(=O)ON1C(=O)CCC1=O OHOQEZWSNFNUSY-UHFFFAOYSA-N 0.000 description 1
- 206010067477 Cytogenetic abnormality Diseases 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 1
- 102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 description 1
- 241001295925 Gegenes Species 0.000 description 1
- 241000882860 Hoolock hoolock Species 0.000 description 1
- 241000282619 Hylobates lar Species 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 241000270322 Lepidosauria Species 0.000 description 1
- 241000282553 Macaca Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241000282537 Mandrillus sphinx Species 0.000 description 1
- 208000001804 Monosomy 5p Diseases 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000282520 Papio Species 0.000 description 1
- 241000288935 Platyrrhini Species 0.000 description 1
- 208000020584 Polyploidy Diseases 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 241000087406 Ponginae Species 0.000 description 1
- 241000282569 Pongo Species 0.000 description 1
- 241000282405 Pongo abelii Species 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- CGNLCCVKSWNSDG-UHFFFAOYSA-N SYBR Green I Chemical compound CN(C)CCCN(CCC)C1=CC(C=C2N(C3=CC=CC=C3S2)C)=C2C=CC=CC2=[N+]1C1=CC=CC=C1 CGNLCCVKSWNSDG-UHFFFAOYSA-N 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 241001501942 Suricata suricatta Species 0.000 description 1
- 206010044688 Trisomy 21 Diseases 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 210000001106 artificial yeast chromosome Anatomy 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000002798 bone marrow cell Anatomy 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 210000002230 centromere Anatomy 0.000 description 1
- 238000000701 chemical imaging Methods 0.000 description 1
- 239000003280 clastogen Substances 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229910001651 emery Inorganic materials 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000019688 fish Nutrition 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 210000004754 hybrid cell Anatomy 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000000155 isotopic effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 241001515942 marmosets Species 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000021121 meiosis Effects 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 239000012120 mounting media Substances 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 238000011176 pooling Methods 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000009717 reactive processing Methods 0.000 description 1
- 230000008521 reorganization Effects 0.000 description 1
- 108091035233 repetitive DNA sequence Proteins 0.000 description 1
- 102000053632 repetitive DNA sequence Human genes 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 210000003765 sex chromosome Anatomy 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Steroid Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Treating Waste Gases (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Tires In General (AREA)
- Bridges Or Land Bridges (AREA)
- Earth Drilling (AREA)
- Road Signs Or Road Markings (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring And Recording Apparatus For Diagnosis (AREA)
- Devices For Conveying Motion By Means Of Endless Flexible Members (AREA)
- Mechanical Treatment Of Semiconductor (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9704054.7A GB9704054D0 (en) | 1997-02-27 | 1997-02-27 | Assay |
| GB9704054 | 1997-02-27 | ||
| PCT/IB1998/000439 WO1998038333A1 (en) | 1997-02-27 | 1998-02-27 | Cross-species chromosome painting |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69829860D1 DE69829860D1 (de) | 2005-05-25 |
| DE69829860T2 true DE69829860T2 (de) | 2005-09-22 |
Family
ID=10808389
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69829860T Expired - Fee Related DE69829860T2 (de) | 1997-02-27 | 1998-02-27 | Spezies-übergreifendes anfärben von chromosomen |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US6255465B1 (enExample) |
| EP (1) | EP1012330B1 (enExample) |
| JP (1) | JP2001512984A (enExample) |
| AT (1) | ATE293706T1 (enExample) |
| AU (1) | AU6307598A (enExample) |
| CA (1) | CA2282788A1 (enExample) |
| DE (1) | DE69829860T2 (enExample) |
| GB (2) | GB9704054D0 (enExample) |
| WO (1) | WO1998038333A1 (enExample) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0878552A1 (en) * | 1997-05-13 | 1998-11-18 | Erasmus Universiteit Rotterdam | Molecular detection of chromosome aberrations |
| GB2330907A (en) * | 1997-10-28 | 1999-05-05 | Applied Imaging Int Ltd | A karyotyper and methods for producing karyotypes |
| US6043039A (en) * | 1998-02-17 | 2000-03-28 | Applied Spectral Imaging | Method of and composite for in situ fluorescent hybridization |
| JP2002513587A (ja) | 1998-05-04 | 2002-05-14 | ダコ エー エス | 染色体異常を検出するための方法およびプローブ |
| FR2784683B1 (fr) | 1998-10-15 | 2002-12-13 | Genset Sa | Sondes fluorescentes de peinture chromosomique |
| US6174681B1 (en) | 1999-03-05 | 2001-01-16 | Mayo Foundation For Medical Education And Research | Method and probe set for detecting cancer |
| US6941317B1 (en) * | 1999-09-14 | 2005-09-06 | Eragen Biosciences, Inc. | Graphical user interface for display and analysis of biological sequence data |
| DE69940524D1 (de) * | 1999-11-09 | 2009-04-16 | Raytheon Co | Abbildungssystem zum nachweis von fluoreszierenden organischen substanzen in einer probe |
| US6620586B2 (en) * | 2001-02-20 | 2003-09-16 | Applied Gene Technologies, Inc. | Methods and compositions for analyzing nucleic acids |
| US20040166518A1 (en) * | 2003-01-02 | 2004-08-26 | The Regents Of The University Of California | Multilabeling mouse FISH assay for detecting structural and numerical chromosomal abnormalities |
| US7653260B2 (en) | 2004-06-17 | 2010-01-26 | Carl Zeis MicroImaging GmbH | System and method of registering field of view |
| US8582924B2 (en) * | 2004-06-30 | 2013-11-12 | Carl Zeiss Microimaging Gmbh | Data structure of an image storage and retrieval system |
| US8574836B2 (en) | 2005-01-12 | 2013-11-05 | Ramesh Vallabhaneni | Method and apparatus for chromosome profiling |
| US7943304B2 (en) | 2005-01-12 | 2011-05-17 | Ramesh Vallabhaneni | Method and apparatus for chromosome profiling |
| US8278050B2 (en) * | 2006-09-20 | 2012-10-02 | Colorado State University Research Foundation | Detection of chromosomal inversions using non-repetitive nucleic acid probes |
| DK2156370T3 (da) | 2007-05-14 | 2012-01-23 | Historx Inc | Kompartmentadskillelse ved pixelkarakterisering under anvendelse af billeddata-clustering |
| CN101784673B (zh) * | 2007-06-07 | 2013-03-27 | 西蒙斯单倍体有限公司 | 外遗传方法 |
| ES2599902T3 (es) | 2007-06-15 | 2017-02-06 | Novartis Ag | Sistema y método de microscopio para obtener datos de muestra normalizados |
| CA2604317C (en) | 2007-08-06 | 2017-02-28 | Historx, Inc. | Methods and system for validating sample images for quantitative immunoassays |
| CA2596204C (en) | 2007-08-07 | 2019-02-26 | Historx, Inc. | Method and system for determining an optimal dilution of a reagent |
| WO2009029810A1 (en) * | 2007-08-31 | 2009-03-05 | Historx, Inc. | Automatic exposure time selection for imaging tissue |
| US20090111184A1 (en) * | 2007-10-24 | 2009-04-30 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Chromosome selection |
| US20090111764A1 (en) * | 2007-10-25 | 2009-04-30 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Mitochondrial selection |
| US20090111185A1 (en) * | 2007-10-26 | 2009-04-30 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Female genome selection |
| CN102165489B (zh) | 2008-09-16 | 2015-11-25 | 赫斯托克斯公司 | 生物标志物表达的可再现量化 |
| US8774488B2 (en) | 2010-03-11 | 2014-07-08 | Cellscape Corporation | Method and device for identification of nucleated red blood cells from a maternal blood sample |
| US9090935B2 (en) | 2010-10-11 | 2015-07-28 | Kromatid, Inc. | Characterization of chromosomal inversions using anti-parallel probes |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5447841A (en) | 1986-01-16 | 1995-09-05 | The Regents Of The Univ. Of California | Methods for chromosome-specific staining |
| US5784162A (en) | 1993-08-18 | 1998-07-21 | Applied Spectral Imaging Ltd. | Spectral bio-imaging methods for biological research, medical diagnostics and therapy |
| US5936731A (en) * | 1991-02-22 | 1999-08-10 | Applied Spectral Imaging Ltd. | Method for simultaneous detection of multiple fluorophores for in situ hybridization and chromosome painting |
| US5817462A (en) | 1995-02-21 | 1998-10-06 | Applied Spectral Imaging | Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding |
| US5719024A (en) | 1993-08-18 | 1998-02-17 | Applied Spectral Imaging Ltd. | Method for chromosome classification by decorrelation statistical analysis and hardware therefore |
| US5798262A (en) | 1991-02-22 | 1998-08-25 | Applied Spectral Imaging Ltd. | Method for chromosomes classification |
| ES2161715T3 (es) | 1992-03-04 | 2001-12-16 | Univ California | Hibridacion genomica comparativa (hgc). |
| US5843649A (en) * | 1995-03-01 | 1998-12-01 | University Of Colorado | Method of identifying clonal cell samples using heteroduplex generators |
-
1997
- 1997-02-27 GB GBGB9704054.7A patent/GB9704054D0/en active Pending
-
1998
- 1998-02-27 US US09/032,346 patent/US6255465B1/en not_active Expired - Fee Related
- 1998-02-27 AU AU63075/98A patent/AU6307598A/en not_active Abandoned
- 1998-02-27 WO PCT/IB1998/000439 patent/WO1998038333A1/en not_active Ceased
- 1998-02-27 JP JP53745998A patent/JP2001512984A/ja not_active Ceased
- 1998-02-27 CA CA002282788A patent/CA2282788A1/en not_active Abandoned
- 1998-02-27 DE DE69829860T patent/DE69829860T2/de not_active Expired - Fee Related
- 1998-02-27 GB GB9811526A patent/GB2325302B/en not_active Expired - Fee Related
- 1998-02-27 AT AT98907137T patent/ATE293706T1/de not_active IP Right Cessation
- 1998-02-27 EP EP98907137A patent/EP1012330B1/en not_active Expired - Lifetime
-
1999
- 1999-06-30 US US09/337,013 patent/US6270971B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| GB2325302A (en) | 1998-11-18 |
| ATE293706T1 (de) | 2005-05-15 |
| DE69829860D1 (de) | 2005-05-25 |
| US6270971B1 (en) | 2001-08-07 |
| GB9704054D0 (en) | 1997-04-16 |
| JP2001512984A (ja) | 2001-08-28 |
| EP1012330B1 (en) | 2005-04-20 |
| GB9811526D0 (en) | 1998-07-29 |
| GB2325302B (en) | 2001-08-08 |
| WO1998038333A1 (en) | 1998-09-03 |
| AU6307598A (en) | 1998-09-18 |
| CA2282788A1 (en) | 1998-09-03 |
| EP1012330A1 (en) | 2000-06-28 |
| US6255465B1 (en) | 2001-07-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69829860T2 (de) | Spezies-übergreifendes anfärben von chromosomen | |
| DE69032920T2 (de) | Verfahren und Zusammensetzungen für chromosomenspezifische Färbung | |
| DE68927059T2 (de) | In-situ-unterdrückungs-hybridisierung und verwendungen | |
| DE69215533T2 (de) | In-situ-hybridisierungsmethode | |
| DE69330750T2 (de) | Vergleichende genomhybridisierung | |
| US6132961A (en) | Methods of biological dosimetry employing chromosome-specific staining | |
| DE69534046T2 (de) | Vergleichende hybridisierung mit nukleinsäureanordnungen | |
| DE69620791T2 (de) | Verfahren zur auswertung polymorpher genetischer sequenzen, und dessen gebrauch zur bestimmung von hla-typen | |
| DE69737398T2 (de) | Genetische Veränderungen im Zusammenhang mit Prostatakrebs | |
| DE69531133T2 (de) | Verfahren zur Dämpfung der Fluoreszene in Lösung von Fluoreszenzmarkierten Oligonucleotidsonden | |
| DE69733958T2 (de) | Verfahren zur positionierung von klonen mittels molekularen kaemmens | |
| DE69900949T2 (de) | Dns amplifizierung aus einzelnen zellen | |
| US6280929B1 (en) | Method of detecting genetic translocations identified with chromosomal abnormalities | |
| US6500612B1 (en) | Methods and compositions for chromosome 21-specific staining | |
| Lichter et al. | [22] Detection of chromosomal aberrations by means of molecular cytogenetics: Painting of chromosomes and chromosomal subregions and comparative genomic hybridization | |
| EP0731849A1 (de) | Anordnung von nukleinsäuresequenzen und deren verwendung | |
| WO1994028178A1 (en) | In-situ hybridization probes for identification and banding of specific human chromosomes and regions | |
| US6203977B1 (en) | Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization | |
| DE69620413T2 (de) | Verfahren zur bestimmung des hautfärbung-genotyps eines schweins | |
| DE69937837T2 (de) | Fluoreszenzsonden zum anfärben von chromosomen | |
| Carter | Fluorescence in situ hybridization—state of the art | |
| Van Dekken et al. | Enzymatic production of single-stranded DNA as a target for fluorescence in situ hybridization | |
| DE19610255B4 (de) | Verfahren zur Herstellung von Nukleinsäuresequenzen und Verfahren zum Nachweis von Translokationen zwischen Chromosomen | |
| DE69232842T2 (de) | Herstellungsmethode für chromosomenregion-spezifische proben | |
| US20040235039A1 (en) | Chromosome-specific staining to detect genetic rearrangements |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |