DE69821540D1 - Mit einem Adapter versehene kompetitive PCR - Google Patents

Mit einem Adapter versehene kompetitive PCR

Info

Publication number
DE69821540D1
DE69821540D1 DE69821540T DE69821540T DE69821540D1 DE 69821540 D1 DE69821540 D1 DE 69821540D1 DE 69821540 T DE69821540 T DE 69821540T DE 69821540 T DE69821540 T DE 69821540T DE 69821540 D1 DE69821540 D1 DE 69821540D1
Authority
DE
Germany
Prior art keywords
adapter
competitive pcr
competitive
pcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69821540T
Other languages
English (en)
Other versions
DE69821540T2 (de
Inventor
Kikuya Kato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Science and Technology Agency
Original Assignee
Japan Science and Technology Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Science and Technology Corp filed Critical Japan Science and Technology Corp
Publication of DE69821540D1 publication Critical patent/DE69821540D1/de
Application granted granted Critical
Publication of DE69821540T2 publication Critical patent/DE69821540T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69821540T 1997-04-07 1998-04-07 Mit einem Adapter versehene kompetitive PCR Expired - Fee Related DE69821540T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP8849597 1997-04-07
JP8849597 1997-04-07

Publications (2)

Publication Number Publication Date
DE69821540D1 true DE69821540D1 (de) 2004-03-18
DE69821540T2 DE69821540T2 (de) 2004-12-30

Family

ID=13944405

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69821540T Expired - Fee Related DE69821540T2 (de) 1997-04-07 1998-04-07 Mit einem Adapter versehene kompetitive PCR

Country Status (3)

Country Link
US (1) US6090556A (de)
EP (1) EP0870842B1 (de)
DE (1) DE69821540T2 (de)

Families Citing this family (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6248534B1 (en) * 1999-05-25 2001-06-19 Kikuya Kato Method for determining the detection value of a target RNA
GB2365124B (en) * 2000-07-21 2002-05-01 Karolinska Innovations Ab Methods for analysis and identification of transcribed genes and fingerprinting
IL155450A0 (en) * 2000-10-20 2003-11-23 Expression Diagnostics Inc Leukocyte expression profiling
IL155447A0 (en) 2000-10-20 2003-11-23 Eisai Co Ltd Nitrogenous aromatic ring compounds
WO2002061145A2 (en) * 2001-01-31 2002-08-08 Ambion, Inc. Competitive amplification of fractionated targets from multiple nucleic acid samples
WO2002064835A2 (en) * 2001-01-31 2002-08-22 Ambion, Inc. Methods for nucleic acid fingerprint analysis
WO2002061140A2 (en) * 2001-01-31 2002-08-08 Ambion, Inc. Competitive population normalization for comparative analysis of nucleic acid samples
WO2002061143A2 (en) * 2001-01-31 2002-08-08 Ambion, Inc. Comparative analysis of nucleic acids using population tagging
WO2002090564A1 (en) * 2001-05-07 2002-11-14 Fox Chase Cancer Center Methods for gene expression profiling
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
WO2002103007A1 (fr) * 2001-06-19 2002-12-27 Eisai C0. Ltd. Methode d'uniformisation des contenus de fragments d'adn et methode de soustraction
US20030170700A1 (en) * 2002-01-09 2003-09-11 Lynx Therapeutics, Inc. Secreted and cell surface polypeptides affected by cholesterol and uses thereof
US20030166026A1 (en) * 2002-01-09 2003-09-04 Lynx Therapeutics, Inc. Identification of specific biomarkers for breast cancer cells
CN1182256C (zh) * 2002-08-09 2004-12-29 周国华 基因表达量比较分析法
JP2004113108A (ja) * 2002-09-26 2004-04-15 Aisin Seiki Co Ltd Ｄｎａ回収法
WO2004065628A1 (en) * 2003-01-21 2004-08-05 Guoliang Fu Quantitative multiplex detection of nucleic acids
AU2003202671A1 (en) * 2003-01-21 2004-08-13 Guoliang Fu Quantitative multiplex detection of nucleic acids
JPWO2004080462A1 (ja) 2003-03-10 2006-06-08 エーザイ株式会社 ｃ−Ｋｉｔキナーゼ阻害剤
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
EP1683785B1 (de) 2003-11-11 2013-10-16 Eisai R&D Management Co., Ltd. Harnstoffderivate und verfahren zu deren herstellung
EP2290069A3 (de) 2004-05-28 2011-08-10 Asuragen, Inc. Verfahren und Zusammensetzungen mit MicroRNA
US7645575B2 (en) 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
AU2005283422C1 (en) 2004-09-17 2017-02-02 Eisai R & D Management Co., Ltd. Medicinal composition
EP2808389A1 (de) 2004-11-12 2014-12-03 Asuragen, Inc. Verfahren und Zusammensetzungen mit miRNA und miRNA-Inhibitormolekülen
WO2006102497A2 (en) * 2005-03-22 2006-09-28 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for diagnosis, monitoring and development of therapeutics for treatment of atherosclerotic disease
CN102925561B (zh) 2005-06-23 2015-09-09 科因股份有限公司 用于多态性的高通量鉴定和检测的策略
EP2281901B1 (de) 2005-08-02 2013-11-27 Eisai R&D Management Co., Ltd. Pharmazeutische anti-tumor Zusammensetzung mit Angiogeneseinhibitoren
CN1908189A (zh) * 2005-08-02 2007-02-07 博奥生物有限公司 体外辅助鉴定肠型胃癌及其分化程度的方法与专用试剂盒
US10316364B2 (en) 2005-09-29 2019-06-11 Keygene N.V. Method for identifying the source of an amplicon
ATE453728T1 (de) 2005-09-29 2010-01-15 Keygene Nv Screening mutagenisierter populationen mit hohem durchsatz
WO2007063807A1 (ja) * 2005-11-29 2007-06-07 Olympus Corporation 核酸の一次構造変化の解析方法
EP3404114B1 (de) 2005-12-22 2021-05-05 Keygene N.V. Verfahren zur auf aflp basierenden hochdurchsatz-polymorphismusdetektion
PT2963127T (pt) 2006-04-04 2017-10-06 Keygene Nv Detecção de alto rendimento de marcadores moleculares com base em fragmentos de restrição
US20090209580A1 (en) 2006-05-18 2009-08-20 Eisai R & D Management Co., Ltd. Antitumor agent for thyroid cancer
WO2008021431A2 (en) 2006-08-14 2008-02-21 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
JP5368096B2 (ja) 2006-08-28 2013-12-18 エーザイ・アール・アンド・ディー・マネジメント株式会社 未分化型胃癌に対する抗腫瘍剤
US8148067B2 (en) 2006-11-09 2012-04-03 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US20080187928A1 (en) * 2006-12-29 2008-08-07 The Salk Institute For Biological Studies Methods for enhancing exercise performance
WO2008093855A1 (ja) 2007-01-29 2008-08-07 Eisai R & D Management Co., Ltd. 未分化型胃癌治療用組成物
US20080274458A1 (en) * 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
US8361714B2 (en) 2007-09-14 2013-01-29 Asuragen, Inc. Micrornas differentially expressed in cervical cancer and uses thereof
KR101513326B1 (ko) 2007-11-09 2015-04-17 에자이 알앤드디 매니지먼트 가부시키가이샤 혈관 신생 저해 물질과 항종양성 백금 착물의 병용
US8071562B2 (en) * 2007-12-01 2011-12-06 Mirna Therapeutics, Inc. MiR-124 regulated genes and pathways as targets for therapeutic intervention
US8258111B2 (en) 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
ES2573515T3 (es) 2010-06-25 2016-06-08 Eisai R&D Management Co., Ltd. Agente antitumoral que emplea compuestos con efecto inhibitorio de cinasas combinados
CN105969773A (zh) 2011-02-03 2016-09-28 米尔纳医疗股份有限公司 Mir-124的合成模拟物
CN103402519B (zh) 2011-04-18 2015-11-25 卫材R&D管理有限公司 肿瘤治疗剂
WO2012166899A2 (en) 2011-06-03 2012-12-06 Eisai R&D Management Co., Ltd. Biomarkers for predicting and assessing responsiveness of thyroid and kidney cancer subjects to lenvatinib compounds
WO2013040251A2 (en) 2011-09-13 2013-03-21 Asurgen, Inc. Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
US10100092B2 (en) 2011-11-03 2018-10-16 Vca, Inc. Compositions and methods to detect various infectious organisms
EP2800820B1 (de) 2011-12-19 2020-04-08 Valley Health System Verfahren und kits zur erkennung von patienten mit bauchspeicheldrüsenkrebs
EP3182126A3 (de) 2012-06-15 2017-08-02 Wayne State University Biomarkertest zur vorhersage oder früherkennung von präeklampsie und/oder hellp-syndrom
EP3339861B8 (de) 2012-06-15 2023-11-01 Genesis Theranostix Korlatolt Felelossegu Tarsasag Biomarkertest zur vorhersage oder früherkennung von präeklampsie
CN110037999B (zh) 2012-08-13 2023-01-13 洛克菲勒大学 治疗和诊断黑素瘤
EP3736022A3 (de) 2012-10-31 2021-01-20 The Rockefeller University Behandlung und diagnose von darmkrebs
KR20150098605A (ko) 2012-12-21 2015-08-28 에자이 알앤드디 매니지먼트 가부시키가이샤 퀴놀린 유도체의 비정질 형태 및 그의 제조방법
CA2912219C (en) 2013-05-14 2021-11-16 Eisai R&D Management Co., Ltd. Biomarkers for predicting and assessing responsiveness of endometrial cancer subjects to lenvatinib compounds
WO2015187612A1 (en) 2014-06-02 2015-12-10 Valley Health System Method and systems for lung cancer diagnosis
KR102512940B1 (ko) 2014-08-28 2023-03-23 에자이 알앤드디 매니지먼트 가부시키가이샤 고순도의 퀴놀린 유도체 및 이를 제조하는 방법
JP6792546B2 (ja) 2015-02-25 2020-11-25 エーザイ・アール・アンド・ディー・マネジメント株式会社 キノリン誘導体の苦味抑制方法
WO2016140717A1 (en) 2015-03-04 2016-09-09 Merck Sharp & Dohme Corp. Combination of a pd-1 antagonist and a vegfr/fgfr/ret tyrosine kinase inhibitor for treating cancer
US11369623B2 (en) 2015-06-16 2022-06-28 Prism Pharma Co., Ltd. Anticancer combination of a CBP/catenin inhibitor and an immune checkpoint inhibitor
WO2019104062A1 (en) 2017-11-21 2019-05-31 Rgenix, Inc. Polymorphs and uses thereof
EP3660172A1 (de) 2018-11-28 2020-06-03 Bioscreening and Diagnostics LLC Verfahren zur diagnose von traumatischen hirnverletzungen
AU2020401286A1 (en) 2019-12-13 2022-06-23 Inspirna, Inc. Metal salts and uses thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ291877B6 (cs) * 1991-09-24 2003-06-18 Keygene N.V. Způsob amplifikace přinejmenším jednoho restrikčního fragmentu z výchozí DNA a způsob přípravy sestavy amplifikovaných restrikčních fragmentů
US5643765A (en) * 1993-04-06 1997-07-01 University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
RU2111254C1 (ru) * 1994-07-11 1998-05-20 Институт молекулярной биологии им.В.А.Энгельгардта РАН СПОСОБ ДЕТЕКЦИИ ДИФФЕРЕНЦИАЛЬНО ЭКСПРЕССИРУЮЩИХСЯ МАТРИЧНЫХ РНК И КЛОНИРОВАНИЯ СООТВЕТСТВУЮЩИХ ИМ ФРАГМЕНТОВ кДНК
DE69621507T2 (de) * 1995-03-28 2003-01-09 Japan Science & Tech Corp Verfahren zur molekularen Indexierung von Genen unter Verwendung von Restriktionsenzymen
US5712126A (en) * 1995-08-01 1998-01-27 Yale University Analysis of gene expression by display of 3-end restriction fragments of CDNA

Also Published As

Publication number Publication date
EP0870842A3 (de) 1999-01-20
EP0870842A2 (de) 1998-10-14
US6090556A (en) 2000-07-18
EP0870842B1 (de) 2004-02-11
DE69821540T2 (de) 2004-12-30

Similar Documents

Publication Publication Date Title
DE69821540D1 (de) Mit einem Adapter versehene kompetitive PCR
DE69812097D1 (de) Zahnbürste mit rippen
DE59708378D1 (de) Instrument mit einem abwinkelbaren handgriff
DE69721343D1 (de) FPGA mit Setz-/Rücksetzleitungen
PT996701E (pt) Polialquilenoiminas alcoxiladas compativeis com lixivia
DE69906019T2 (de) Zentrifuge mit konischen Trennwänden
DE69931563D1 (de) Zentrifuge mit konischen Trennwänden
NO990643L (no) Tienopyrimidiner
FI973883A0 (fi) Foerfarande foer bestaemning av laeget foer en huvudanordning samt cellulaert radiosystem
NO984536L (no) Skruforbindelse
FI960090A0 (fi) Naetadapter samt dataoeverfoeringsfoerfarande i mobiltelenaet
ID30114A (id) Zat-zat herbisida dengan fenoksisulfonilurea tersubstitusi
NO20002702D0 (no) Tienopyrimidiner
DE69824112D1 (de) Gewindeeinsatz
FI972867A0 (fi) Foerfarande foer maetning av stroemning samt stroemningsmaetare
DE69802265D1 (de) Reifen mit mehreren nichtleitenden mischungen
FI972172A0 (fi) Foerfarande foer maetning av gashalt samt gashaltsmaetare
DE29718238U1 (de) Pipette
DE69829836D1 (de) Polyolefinharzzusammensetzungen
DE69714970D1 (de) Hydroismerisierung mit verminderten hydrokracken
DE59702616D1 (de) Verzögerungsstufe mit steilen flanken
DE29701321U1 (de) Verschraubung
DE29711173U1 (de) Adapter
DE59811880D1 (de) Anschlussadapter
DE29715455U1 (de) Vielzweck-Schraubennuß

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee