DE69738648T2 - Formulierungen vernetzter proteinkristalle und ihre verwendung als katalysatoren in organischen lösungsmitteln - Google Patents

Formulierungen vernetzter proteinkristalle und ihre verwendung als katalysatoren in organischen lösungsmitteln Download PDF

Info

Publication number
DE69738648T2
DE69738648T2 DE69738648T DE69738648T DE69738648T2 DE 69738648 T2 DE69738648 T2 DE 69738648T2 DE 69738648 T DE69738648 T DE 69738648T DE 69738648 T DE69738648 T DE 69738648T DE 69738648 T2 DE69738648 T2 DE 69738648T2
Authority
DE
Germany
Prior art keywords
formulation
organic solvent
crosslinked
protein crystals
crystals
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69738648T
Other languages
German (de)
English (en)
Other versions
DE69738648D1 (de
Inventor
Nazer K. Worcester KHALAF
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Altus Biologics Inc
Original Assignee
Altus Biologics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Altus Biologics Inc filed Critical Altus Biologics Inc
Application granted granted Critical
Publication of DE69738648D1 publication Critical patent/DE69738648D1/de
Publication of DE69738648T2 publication Critical patent/DE69738648T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69738648T 1996-05-24 1997-05-20 Formulierungen vernetzter proteinkristalle und ihre verwendung als katalysatoren in organischen lösungsmitteln Expired - Lifetime DE69738648T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/652,964 US5932212A (en) 1996-05-24 1996-05-24 Crosslinked protein crystal formulations and their use as catalysts in organic solvents
US652964 1996-05-24
PCT/US1997/008526 WO1997044445A1 (en) 1996-05-24 1997-05-20 Cross-linked protein crystal formulations and their use as catalysts in organic solvents

Publications (2)

Publication Number Publication Date
DE69738648D1 DE69738648D1 (de) 2008-06-05
DE69738648T2 true DE69738648T2 (de) 2009-05-28

Family

ID=24618946

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69738648T Expired - Lifetime DE69738648T2 (de) 1996-05-24 1997-05-20 Formulierungen vernetzter proteinkristalle und ihre verwendung als katalysatoren in organischen lösungsmitteln

Country Status (13)

Country Link
US (2) US5932212A (US06373033-20020416-M00035.png)
EP (1) EP0906417B1 (US06373033-20020416-M00035.png)
JP (2) JP4279351B2 (US06373033-20020416-M00035.png)
KR (1) KR20000015993A (US06373033-20020416-M00035.png)
AR (1) AR007211A1 (US06373033-20020416-M00035.png)
AT (1) ATE393216T1 (US06373033-20020416-M00035.png)
AU (1) AU3072897A (US06373033-20020416-M00035.png)
DE (1) DE69738648T2 (US06373033-20020416-M00035.png)
DK (1) DK0906417T3 (US06373033-20020416-M00035.png)
ID (1) ID17817A (US06373033-20020416-M00035.png)
IN (1) IN191060B (US06373033-20020416-M00035.png)
WO (1) WO1997044445A1 (US06373033-20020416-M00035.png)
ZA (1) ZA974325B (US06373033-20020416-M00035.png)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992009750A1 (en) * 1990-11-29 1992-06-11 Willis Richard R Floating contaminant containment systems
US6140475A (en) * 1997-04-11 2000-10-31 Altus Biologics Inc. Controlled dissolution crosslinked protein crystals
JP4433100B2 (ja) * 1997-06-20 2010-03-17 ニューヨーク ユニヴァーシティ チップ及びライブラリの大量製造における物質溶液の静電噴霧
US6833275B1 (en) * 1997-07-22 2004-12-21 Roche Diagnostics Gmbh Gold conjugates containing detergent
EP1009759B1 (en) * 1997-09-05 2008-05-28 Altus Pharmaceuticals Inc. Carbohydrate crosslinked glycoprotein crystals
US6500933B1 (en) 1997-09-05 2002-12-31 Altus Biologics Inc. Methods of preparing carbohydrate crosslinked glycoprotein crystals
MY131805A (en) * 1997-09-18 2007-09-28 Biogen Idec Inc Synergistic composition and methods for treating neoplastic or cancerous growths and for restoring or boosting hematopoiesis.
US6541606B2 (en) * 1997-12-31 2003-04-01 Altus Biologics Inc. Stabilized protein crystals formulations containing them and methods of making them
EP1073421A1 (en) * 1998-04-27 2001-02-07 Altus Biologics Inc. Stabilized protein crystals, formulations containing them and methods of making them
ES2386535T3 (es) * 1998-10-09 2012-08-22 Althea Technologies, Inc. Sistemas no homogéneos para la resolución de mezclas enantioméricas
US6979561B1 (en) 1998-10-09 2005-12-27 Gilead Sciences, Inc. Non-homogeneous systems for the resolution of enantiomeric mixtures
DE19903125B4 (de) * 1999-01-27 2006-01-05 Sanofi-Aventis Deutschland Gmbh Verfahren zur Trocknung von Kristallen von Insulin oder Insulinanaloga
WO2001012834A1 (en) * 1999-08-18 2001-02-22 Hydrios Biotechnology Oy Process for the preparation and simultaneous separation of enzyme-catalysed products
US7521216B2 (en) 1999-12-29 2009-04-21 Verenium Corporation Nitrilases and methods for making and using them
US20040014195A1 (en) * 1999-12-29 2004-01-22 Diversa Corporation Nitrilases, nucleic acids encoding them and methods for making and using them
US7608445B1 (en) 1999-12-29 2009-10-27 Verenium Corporation Nitrilases, nucleic acids encoding them and methods for making and using them
US7300775B2 (en) * 1999-12-29 2007-11-27 Verenium Corporation Methods for producing α-substituted carboxylic acids using nitrilases and strecker reagents
US20010046493A1 (en) * 2000-02-24 2001-11-29 Alex Margolin Lipase-containing composition and methods of use thereof
EP1292823A1 (en) * 2000-05-23 2003-03-19 Radiometer Medical A/S A sensor membrane, a method for the preparation thereof, a sensor and a layered membrane structure for such sensor
NZ526720A (en) * 2000-12-28 2007-11-30 Altus Pharmaceuticals Inc Crystallisation of whole antibodies or fragments thereof, on a large scale and a process allowing an alternative route for delivery of therapeutic antibodies
AU2002322295C1 (en) 2001-06-21 2008-12-18 Altus Pharmaceuticals Inc. Spherical protein particles and methods of making and using them
AU2003251523A1 (en) 2002-06-13 2003-12-31 Diversa Corporation Processes for making (r)-ethyl 4-cyano-3-hydroxybutyric acid
US9035123B2 (en) * 2002-10-01 2015-05-19 The Procter & Gamble Company Absorbent article having a lotioned topsheet
NZ571243A (en) * 2002-12-31 2010-04-30 Altus Pharmaceuticals Inc Complexes of protein crystals and ionic polymers comprising human growth hormone and protamine
BR0317888A (pt) * 2002-12-31 2005-12-06 Altus Pharmaceuticals Inc Cristais do hormÈnio do crescimento humano e processos para preparação dos mesmos
JP4799422B2 (ja) * 2004-01-01 2011-10-26 カウンシル・オブ・サイエンティフィック・アンド・インダストリアル・リサーチ 架橋タンパク質結晶の調製方法
PL2198880T3 (pl) * 2004-10-14 2017-04-28 Eli Lilly And Co. Kompozycje zawierające lipazę, proteazę i amylazę do leczenia niewydolności trzustki
WO2007050100A2 (en) * 2004-11-24 2007-05-03 Industrial Science & Technology Network, Inc. Immobilized enzymes and processes for preparing and using same
US20060223765A1 (en) * 2005-03-30 2006-10-05 Kimberly-Clark Worldwide, Inc. Method for inhibiting and/or treating vaginal infection
GB0513888D0 (en) * 2005-07-06 2005-08-10 Btg Int Ltd Core 2 GLCNAC-T Inhibitors II
US20100196344A1 (en) * 2005-10-14 2010-08-05 Cystic Fibrosis Foundation Therapeutics, Inc. Compositions and methods for treating pancreatic insufficiency
US20090023629A1 (en) * 2005-12-23 2009-01-22 Altus Pharmaceuticals Inc. Compositions comprising polycation-complexed protein crystals and methods of treatment using them
US20100047413A1 (en) * 2006-09-15 2010-02-25 Cabalka Tourtellotte Leanne M Method of using amphiphilic multiblock copolymers in food
EP2115153B1 (en) 2007-03-01 2013-06-05 BP Corporation North America Inc. Nitrilases, nucleic acids encoding them and methods for making and using them
IL183084A0 (en) * 2007-05-09 2007-09-20 Trans Biodisel Ltd Modified-immobilized enzymes of high tolerance to hydrophilic substrates in organic media
CN101717766B (zh) * 2009-12-01 2012-07-18 暨南大学 蛋白质酶的微水介质固定化方法
US8354262B2 (en) 2010-06-30 2013-01-15 Codexis, Inc. Chemically modified carbonic anhydrases useful in carbon capture systems
WO2012026493A1 (ja) * 2010-08-26 2012-03-01 アイシン精機株式会社 酵素結晶固定化電極及び酵素結晶固定化電極の製造方法、並びに酵素結晶固定化電極を備えるバイオ電池及びバイオセンサー

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4267234A (en) * 1978-03-17 1981-05-12 California Institute Of Technology Polyglutaraldehyde synthesis and protein bonding substrates
US4369226A (en) * 1979-03-19 1983-01-18 California Institute Of Technology Polyglutaraldehyde synthesis and protein bonding substrates
US5152903A (en) * 1988-12-19 1992-10-06 American Cyanamid Company Cross-linked cationic polymeric microparticles
ATE240391T1 (de) * 1990-08-03 2003-05-15 Vertex Pharma Verwendung von vernetzten kristallen als neuem verfahren zur immobilisierung von enzymen
GB9309243D0 (en) * 1993-05-05 1993-06-16 Allied Colloids Ltd Enzyme dispersions,their production and compositions containing them
ATE160582T1 (de) * 1993-05-20 1997-12-15 Loders Croklaan Bv Verfahren zur herstellung immobilisierter lipasen
BR9408185A (pt) * 1993-12-03 1997-05-27 Buckman Labor Inc Método para estabilizar uma composiçao de enzima e composiçao de enzima
DE4344211A1 (de) * 1993-12-23 1995-06-29 Basf Ag Verfahren zur Erhöhung der Aktivität von hydrolytischen Enzymen

Also Published As

Publication number Publication date
DK0906417T3 (da) 2008-07-21
EP0906417A1 (en) 1999-04-07
JP2008061652A (ja) 2008-03-21
IN191060B (US06373033-20020416-M00035.png) 2003-09-13
ID17817A (id) 1998-01-29
US5932212A (en) 1999-08-03
AR007211A1 (es) 1999-10-13
AU3072897A (en) 1997-12-09
JP4279351B2 (ja) 2009-06-17
ATE393216T1 (de) 2008-05-15
US6042824A (en) 2000-03-28
EP0906417B1 (en) 2008-04-23
WO1997044445A1 (en) 1997-11-27
KR20000015993A (ko) 2000-03-25
JP2000514282A (ja) 2000-10-31
DE69738648D1 (de) 2008-06-05
ZA974325B (en) 1997-12-18

Similar Documents

Publication Publication Date Title
DE69738648T2 (de) Formulierungen vernetzter proteinkristalle und ihre verwendung als katalysatoren in organischen lösungsmitteln
EP0140542B2 (en) An immoblized lipase preparation and use thereof
US5108916A (en) Process for stereoselectively hydrolyzing, transesterifying or esterifying with immobilized isozyme of lipase from candida rugosa
DE19931847A1 (de) Immobilisierte Lipase
DE69914458T2 (de) Verfahren zur enzymatischen auflösung von laktamen
US5508182A (en) Esterification of hydrophilic polyols by adsorption onto a solid support and employing a substrate-immiscible solvent
JPH0279995A (ja) 酵素的精製方法
DE68924347T2 (de) Enzymatische auflösungssysteme und in diesen systemen angewandte verbindungen und ihre herstellungen.
DK152763B (da) Fremgangsmaade til fremstilling af et immobiliseret lipasepraeparat
EP0349606B1 (en) Process for producing highly pure oleic acid by hydrolysis of sunflower oil
US4013512A (en) Method for adsorption and elution of lipid hydrolyzing enzymes
US20090035828A1 (en) Method For the Preparation of Cross Linked Protein Crystals
DE69203718T2 (de) Enzymatische reverse-hydrolyse von hydrophilen substraten; herstellung von amphiphilen verbindungen.
AT398081B (de) Verfahren zur enzymatischen hydrolyse eines carbonsäurederivates
DE10220740A1 (de) Verfahren zur enzymatischen Herstellung enantiomerenangereicherter beta-Aminosäuren
DE69016570T2 (de) Enzym auf einem Träger.
US6979561B1 (en) Non-homogeneous systems for the resolution of enantiomeric mixtures
Duan et al. Effects of polar additives on the enzyme enantioselectivity of an esterification reaction in organic solvents
Fukui et al. [27] Optical resolution of dl-menthol by entrapped biocatalysts
EP0648277B1 (de) Mikrobielle esterase zur enantioselektiven spaltung von 1-arylalkylestern
CA2101733C (en) Enzymatic reverse hydrolysis of hydrophilic substrates-preparation of amphiphilic compounds
DE2805366C2 (US06373033-20020416-M00035.png)
JP2792091B2 (ja) １―アシル―リゾホスファチジルコリンの製造方法
JPH03259089A (ja) 有機酸エステルの製造方法
JPH03201996A (ja) 生化学的手法による光学活性第２級アルキンアルコールの製造法

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
R082 Change of representative

Ref document number: 906417

Country of ref document: EP