DE69727500D1 - Verfahren und apparatur zur behandlung von material mit elektrischen feldern unterschiedlicher orientierung - Google Patents

Verfahren und apparatur zur behandlung von material mit elektrischen feldern unterschiedlicher orientierung

Info

Publication number
DE69727500D1
DE69727500D1 DE69727500T DE69727500T DE69727500D1 DE 69727500 D1 DE69727500 D1 DE 69727500D1 DE 69727500 T DE69727500 T DE 69727500T DE 69727500 T DE69727500 T DE 69727500T DE 69727500 D1 DE69727500 D1 DE 69727500D1
Authority
DE
Germany
Prior art keywords
electrode selection
selection apparatus
electrical fields
electrodes
containing material
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69727500T
Other languages
English (en)
Other versions
DE69727500T2 (de
Inventor
Richard E Walters
Alan D King
Derin C Walters
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cyto Pulse Sciences Inc
Original Assignee
Cyto Pulse Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cyto Pulse Sciences Inc filed Critical Cyto Pulse Sciences Inc
Publication of DE69727500D1 publication Critical patent/DE69727500D1/de
Application granted granted Critical
Publication of DE69727500T2 publication Critical patent/DE69727500T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Treatments Of Macromolecular Shaped Articles (AREA)
DE69727500T 1997-06-10 1997-06-10 Verfahren und apparatur zur behandlung von material mit elektrischen feldern unterschiedlicher orientierung Expired - Fee Related DE69727500T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1997/009300 WO1998056893A1 (en) 1997-06-10 1997-06-10 Method and apparatus for treating materials with electrical fields having varying orientations

Publications (2)

Publication Number Publication Date
DE69727500D1 true DE69727500D1 (de) 2004-03-11
DE69727500T2 DE69727500T2 (de) 2004-10-07

Family

ID=22260994

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69727500T Expired - Fee Related DE69727500T2 (de) 1997-06-10 1997-06-10 Verfahren und apparatur zur behandlung von material mit elektrischen feldern unterschiedlicher orientierung

Country Status (6)

Country Link
EP (1) EP0968275B1 (de)
JP (1) JP2001500268A (de)
AT (1) ATE258980T1 (de)
CA (1) CA2263720A1 (de)
DE (1) DE69727500T2 (de)
WO (1) WO1998056893A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9804246D0 (en) * 1998-02-27 1998-04-22 Babraham The Inst Electropermeabilisation method and apparatus
DE19823047C1 (de) 1998-05-22 1999-08-26 Fuhr Verfahren und Vorrichtung zur Permeation biologischer Objekte
US6403348B1 (en) * 1999-07-21 2002-06-11 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
US6300108B1 (en) * 1999-07-21 2001-10-09 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
SE9902817D0 (sv) * 1999-07-30 1999-07-30 A & Science Invest Ab A method for selective electrofusion of at least two fusion partners having cell-like membranes
WO2001023537A1 (en) * 1999-09-24 2001-04-05 Cyto Pulse Sciences, Inc. Process for enhancing electric field-mediated delivery of biological materials into cells
GB0002856D0 (en) * 2000-02-08 2000-03-29 Gendel Limited Ultrasound sensitisation
US6714816B1 (en) * 2000-02-22 2004-03-30 University Of South Florida Electroporation and electrophoresis system and method for achieving molecular penetration into cells in vivo
JP2005511079A (ja) * 2001-12-06 2005-04-28 バイオ−ラッド ラボラトリーズ，インコーポレイティド 自動電気穿孔最適化システム
WO2003102126A1 (en) * 2002-05-31 2003-12-11 Apollo Life Sciences Pty Ltd A method of cell therapy using fused cell hybrids
AU2003254879A1 (en) * 2002-08-08 2004-02-25 Fujisawa Pharmaceutical Co., Ltd. Method of transferring selected molecule into target cells, method of cell-fusing target cells and plasma exposure device to be used in these methods
WO2004074451A2 (en) * 2003-02-18 2004-09-02 Maxcyte, Inc. Loading of cells with antigens by electroporation
CN103275874B (zh) * 2013-06-08 2014-09-10 苏州文曲生物微系统有限公司 一种高密度分布式立体电极装置

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2955076A (en) * 1955-10-05 1960-10-04 Gen Electric Co Ltd Artificial mutation of micro-organisms by electrical shock
DE2502621C3 (de) * 1975-01-23 1978-09-14 Kernforschungsanlage Juelich Gmbh, 5170 Juelich Messung elastischer und dielektrischer Eigenschaften der Membran lebender Zellen
DE3317415A1 (de) * 1983-05-13 1984-11-15 Kernforschungsanlage Jülich GmbH, 5170 Jülich Kammer zur behandlung von zellen im elektrischen feld
JPS60251877A (ja) * 1984-05-30 1985-12-12 Hitachi Ltd 細胞融合装置
JPS60251874A (ja) * 1984-05-30 1985-12-12 Hitachi Ltd 微粒子取扱い装置
JPH0658B2 (ja) * 1984-11-07 1994-01-05 株式会社日立製作所 細胞取扱い装置
JPS6349071A (ja) * 1986-08-19 1988-03-01 Shimadzu Corp 多極電極型細胞融合チヤンバ
US4970154A (en) * 1987-10-09 1990-11-13 Baylor College Of Medicine Method for inserting foreign genes into cells using pulsed radiofrequency

Also Published As

Publication number Publication date
ATE258980T1 (de) 2004-02-15
EP0968275A4 (de) 2002-01-23
DE69727500T2 (de) 2004-10-07
CA2263720A1 (en) 1998-12-17
EP0968275B1 (de) 2004-02-04
EP0968275A1 (de) 2000-01-05
WO1998056893A1 (en) 1998-12-17
JP2001500268A (ja) 2001-01-09

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Legal Events

Date Code Title Description
8339 Ceased/non-payment of the annual fee