DE69723422D1 - Verwendung von multigradienten in gelplatten und kapillaren in der elektrophorese - Google Patents

Verwendung von multigradienten in gelplatten und kapillaren in der elektrophorese

Info

Publication number
DE69723422D1
DE69723422D1 DE69723422T DE69723422T DE69723422D1 DE 69723422 D1 DE69723422 D1 DE 69723422D1 DE 69723422 T DE69723422 T DE 69723422T DE 69723422 T DE69723422 T DE 69723422T DE 69723422 D1 DE69723422 D1 DE 69723422D1
Authority
DE
Germany
Prior art keywords
gradients
capillaries
porosity
electrophoresis
capillary electrophoresis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69723422T
Other languages
English (en)
Other versions
DE69723422T2 (de
Inventor
Pier Giorgio Righetti
Cecilia Gelfi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of DE69723422D1 publication Critical patent/DE69723422D1/de
Application granted granted Critical
Publication of DE69723422T2 publication Critical patent/DE69723422T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44747Composition of gel or of carrier mixture

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
DE69723422T 1996-02-13 1997-02-10 Verwendung von multigradienten in gelplatten und kapillaren in der elektrophorese Expired - Fee Related DE69723422T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ITMI960263 1996-02-13
IT96MI000263A IT1282607B1 (it) 1996-02-13 1996-02-13 Uso di gradienti multipli per l'analisi di mutazioni in dna genomico umano
PCT/EP1997/000622 WO1997030346A1 (en) 1996-02-13 1997-02-10 Creation and use of multiple gradients in electrophoresis in gel slabs and in capillaries

Publications (2)

Publication Number Publication Date
DE69723422D1 true DE69723422D1 (de) 2003-08-14
DE69723422T2 DE69723422T2 (de) 2004-04-15

Family

ID=11373240

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69723422T Expired - Fee Related DE69723422T2 (de) 1996-02-13 1997-02-10 Verwendung von multigradienten in gelplatten und kapillaren in der elektrophorese

Country Status (8)

Country Link
EP (1) EP0880693B1 (de)
JP (1) JP3080995B2 (de)
AT (1) ATE244884T1 (de)
AU (1) AU709906B2 (de)
CA (1) CA2240681C (de)
DE (1) DE69723422T2 (de)
IT (1) IT1282607B1 (de)
WO (1) WO1997030346A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261781B1 (en) * 1997-08-05 2001-07-17 Wisconsin Alumni Research Foundation Direct detection and mutation analysis of low copy number nucleic acids
EP1086248A1 (de) 1998-06-18 2001-03-28 Mosaic Technologies, Inc. Affinitätselektrophorese mit denaturierendem gradienten und verfahren zu deren verwendung
DE10042451A1 (de) * 2000-08-29 2002-03-14 Studiengesellschaft Kohle Mbh High-Throughput Verfahren zur Bestimmung der Enantiomereneinheit von chiralen Verbindungen
CN100357450C (zh) * 2000-10-23 2007-12-26 因哲尼控股公司 检测样品中核酸片段突变的方法和装置
JP4108037B2 (ja) 2001-07-16 2008-06-25 プロテイン, フォレスト, インコーポレイテッド 生体分子の等電点によって生体分子を分析するためのバッファのアレイ

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5190629A (en) * 1987-07-16 1993-03-02 Fuji Photo Film Co., Ltd. Electrophoresis medium membrane
NO169351C (no) * 1987-10-02 1992-06-10 Boerresen Anne Lise Masseundersoekelsesmetode for deteksjon av enkeltbasemutasjoner i multiple loci i genomisk dna ved bruk av denatureringsgradient-gelelektroforese fulgt av effektiv overfoeringog hybridisering til forskjellige prober
US5047134A (en) * 1990-01-25 1991-09-10 Linear Instruments Buffer gradient and temperature gradient capillary electrophoresis

Also Published As

Publication number Publication date
ITMI960263A1 (it) 1997-08-13
ATE244884T1 (de) 2003-07-15
CA2240681A1 (en) 1997-08-21
EP0880693B1 (de) 2003-07-09
WO1997030346A1 (en) 1997-08-21
DE69723422T2 (de) 2004-04-15
JP3080995B2 (ja) 2000-08-28
JPH11509001A (ja) 1999-08-03
AU709906B2 (en) 1999-09-09
EP0880693A1 (de) 1998-12-02
ITMI960263A0 (de) 1996-02-13
IT1282607B1 (it) 1998-03-31
CA2240681C (en) 2004-06-01
AU1768697A (en) 1997-09-02

Similar Documents

Publication Publication Date Title
Jacobson et al. High-speed separations on a microchip
Chen et al. Plastic microchip electrophoresis for analysis of PCR products of hepatitis C virus
Li et al. Clinical analysis by microchip capillary electrophoresis
Zhang et al. Automated and integrated system for high-throughput DNA genotyping directly from blood
Shortreed et al. High-throughput single-molecule DNA screening based on electrophoresis
Xu et al. High-sensitivity capillary gel electrophoretic analysis of DNA fragments on an electrophoresis microchip using electrokinetic injection with transient isotachophoretic preconcentration
Shackman et al. Gradient elution moving boundary electrophoresis for high-throughput multiplexed microfluidic devices
ATE257246T1 (de) Verfahren zur analytischen trennung von viren
Mitchelson The use of capillary electrophoresis for DNA polymorphism analysis
Shackman et al. Gradient elution isotachophoresis for enrichment and separation of biomolecules
Guttman et al. Ultrathin‐layer gel electrophoresis of biopolymers
DE69723422D1 (de) Verwendung von multigradienten in gelplatten und kapillaren in der elektrophorese
US7537680B2 (en) Mixing reactions by temperature gradient focusing
Ferrance et al. Exploiting sensitive laser‐induced fluorescence detection on electrophoretic microchips for executing rapid clinical diagnostics
Woolley Integrating Sample Processing and Detection with Microchip Capillary Electrophoresis of DNA
DE60039792D1 (de) Verfahren und gerät zum nachweis von mutationen in nukleinsäureproben
Ren et al. Inverse-flow derivatization for capillary electrophoresis of DNA fragments with laser-induced fluorescence detection
DE69524945D1 (de) Elektroforetische fluoreszenzvorrichtung für die analyse von polynukleotiden
Kubicki et al. Injection, separation and fluorimetric detection of DNA in glass lab-on-a-chip for capillary gel electrophoresis
Chow Protein separations
Ross et al. 2D separations on a 1D chip: gradient elution moving boundary electrophoresis—chiral capillary zone electrophoresis
Tang et al. Iso-thermal DNA reactions and assays in microfabricated capillary electrophoresis systems
Issaq Parameters affecting capillary electrophoretic separation of DNA
Takagi et al. Application of schlieren optics to, real‐time monitoring of protein electrophoresis in crosslinker‐free linear polyacrylamide solution
Phung et al. Capillary Electrophoresis of Nucleic Acids

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee