NO169351C - Masseundersoekelsesmetode for deteksjon av enkeltbasemutasjoner i multiple loci i genomisk dna ved bruk av denatureringsgradient-gelelektroforese fulgt av effektiv overfoeringog hybridisering til forskjellige prober - Google Patents

Masseundersoekelsesmetode for deteksjon av enkeltbasemutasjoner i multiple loci i genomisk dna ved bruk av denatureringsgradient-gelelektroforese fulgt av effektiv overfoeringog hybridisering til forskjellige prober

Info

Publication number
NO169351C
NO169351C NO874164A NO874164A NO169351C NO 169351 C NO169351 C NO 169351C NO 874164 A NO874164 A NO 874164A NO 874164 A NO874164 A NO 874164A NO 169351 C NO169351 C NO 169351C
Authority
NO
Norway
Prior art keywords
hybridization
effective
mutions
gelelectrophoresis
detection
Prior art date
Application number
NO874164A
Other languages
English (en)
Other versions
NO169351B (no
NO874164D0 (no
NO874164L (no
Inventor
Anne-Lise Boerresen
Original Assignee
Boerresen Anne Lise
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boerresen Anne Lise filed Critical Boerresen Anne Lise
Priority to NO874164A priority Critical patent/NO169351C/no
Publication of NO874164D0 publication Critical patent/NO874164D0/no
Priority to US07/381,405 priority patent/US5190856A/en
Priority to PCT/NO1988/000074 priority patent/WO1989002930A1/en
Publication of NO874164L publication Critical patent/NO874164L/no
Publication of NO169351B publication Critical patent/NO169351B/no
Publication of NO169351C publication Critical patent/NO169351C/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/973—Simultaneous determination of more than one analyte
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/809—Multifield plates or multicontainer arrays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
NO874164A 1987-10-02 1987-10-02 Masseundersoekelsesmetode for deteksjon av enkeltbasemutasjoner i multiple loci i genomisk dna ved bruk av denatureringsgradient-gelelektroforese fulgt av effektiv overfoeringog hybridisering til forskjellige prober NO169351C (no)

Priority Applications (3)

Application Number Priority Date Filing Date Title
NO874164A NO169351C (no) 1987-10-02 1987-10-02 Masseundersoekelsesmetode for deteksjon av enkeltbasemutasjoner i multiple loci i genomisk dna ved bruk av denatureringsgradient-gelelektroforese fulgt av effektiv overfoeringog hybridisering til forskjellige prober
US07/381,405 US5190856A (en) 1987-10-02 1988-10-03 Method and apparatus for detecting single base mutations in dna with denaturing gradient electrophoresis
PCT/NO1988/000074 WO1989002930A1 (en) 1987-10-02 1988-10-03 Method and apparatus for detecting single base mutations in dna with denaturing gradient electrophoresis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NO874164A NO169351C (no) 1987-10-02 1987-10-02 Masseundersoekelsesmetode for deteksjon av enkeltbasemutasjoner i multiple loci i genomisk dna ved bruk av denatureringsgradient-gelelektroforese fulgt av effektiv overfoeringog hybridisering til forskjellige prober

Publications (4)

Publication Number Publication Date
NO874164D0 NO874164D0 (no) 1987-10-02
NO874164L NO874164L (no) 1989-04-03
NO169351B NO169351B (no) 1992-03-02
NO169351C true NO169351C (no) 1992-06-10

Family

ID=19890285

Family Applications (1)

Application Number Title Priority Date Filing Date
NO874164A NO169351C (no) 1987-10-02 1987-10-02 Masseundersoekelsesmetode for deteksjon av enkeltbasemutasjoner i multiple loci i genomisk dna ved bruk av denatureringsgradient-gelelektroforese fulgt av effektiv overfoeringog hybridisering til forskjellige prober

Country Status (3)

Country Link
US (1) US5190856A (no)
NO (1) NO169351C (no)
WO (1) WO1989002930A1 (no)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5633129A (en) * 1989-07-13 1997-05-27 Massachusetts Institute Of Technology Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices
EP0497448A1 (en) * 1991-02-01 1992-08-05 Beckman Instruments, Inc. Capillary gel electrophoresis in the presence of at least one neutral non-urea denaturing agent
FR2686621B1 (fr) * 1992-01-24 1996-03-15 Appligene Sa Procede de detection de mutations par electrophorese en gradient de denaturation d'adn double brin stabilise par photopontage.
US5523208A (en) * 1994-11-30 1996-06-04 The Board Of Trustees Of The University Of Kentucky Method to discover genetic coding regions for complementary interacting proteins by scanning DNA sequence data banks
IT1282607B1 (it) * 1996-02-13 1998-03-31 Pier Giorgio Righetti Uso di gradienti multipli per l'analisi di mutazioni in dna genomico umano
US5994075A (en) * 1996-05-17 1999-11-30 Hexagen Technology Limited Methods for identifying a mutation in a gene of interest without a phenotypic guide
US6015670A (en) * 1996-05-17 2000-01-18 Hexagen Technology Limited Methods for identifying a mutation in a gene of interest without a phenotypic guide using ES cells
US6057106A (en) * 1998-01-23 2000-05-02 Novex Sample buffer and methods for high resolution gel electrophoresis of denatured nucleic acids
US6322976B1 (en) 1998-05-28 2001-11-27 Medical Research Council Compositions and methods of disease diagnosis and therapy
AU760236B2 (en) * 1998-06-18 2003-05-08 Exact Sciences Corporation Denaturing gradient affinity electrophoresis and methods of use thereof
EP1384993A1 (de) * 2002-07-23 2004-01-28 F. Hoffmann-La Roche Ag Verfahren und Vorrichtung zum Lokalisieren und Ausschneiden von Anreicherungen in einem Gel
WO2006091579A2 (en) * 2005-02-22 2006-08-31 Regents Of The University Of Minnesota Identification of a gene associated with spinocerebellar ataxia type 5 and methods of use
CN110272983A (zh) * 2019-06-20 2019-09-24 武汉百翼生物科技有限公司 一种检测单碱基突变的方法及应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4292161A (en) * 1979-01-03 1981-09-29 Hoefer Scientific Instruments Vertical gel slab electrophoresis apparatus
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
US4495279A (en) * 1982-07-27 1985-01-22 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Isoelectric focussing-polynucleotide/polyacrylamide gel electrophoresis
US4622124A (en) * 1985-01-11 1986-11-11 International Biotechnologies, Inc. Device for horizontal electroblotting

Also Published As

Publication number Publication date
WO1989002930A1 (en) 1989-04-06
NO169351B (no) 1992-03-02
NO874164D0 (no) 1987-10-02
US5190856A (en) 1993-03-02
NO874164L (no) 1989-04-03

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